(Circular) (Six-base) MAPSORT of: pFN21AE1911.seq Check: 5910 from: 1 to: 6040 pFN21AE1911 6040 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2726 2726 Size: 6040 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3850 278 Size: 53 83 186 936 2314 2468 Fragments arranged by size: 2468 2314 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3383 1937 Size: 1446 4594 Acc65I G'GTAC_C Cuts at: 2447 3361 2447 Size: 914 5126 AclI AA'CG_TT Cuts at: 1563 4167 4540 1563 Size: 2604 373 3063 Fragments arranged by size: 3063 2604 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2623 4103 5487 5755 1632 Size: 991 1480 1384 268 1917 Fragments arranged by size: 1917 1480 1384 991 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5843 AflIII A'CryG_T Cuts at: 1203 2148 2499 4939 5916 1203 Size: 945 351 2440 977 1327 Fragments arranged by size: 2440 1327 977 945 351 AgeI A'CCGG_T Cuts at: 4871 4871 Size: 6040 AhdI GACnn_n'nnGTC Cuts at: 3259 4769 3259 Size: 1510 4530 AleI CACnn'nnGTG Cuts at: 1166 2540 1166 Size: 1374 4666 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2198 1184 Size: 1014 5026 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3293 3325 3293 Size: 32 6008 AlwNI CAG_nnn'CTG Cuts at: 3133 3783 5355 3133 Size: 650 1572 3818 Fragments arranged by size: 3818 1572 650 ApaI G_GGCC'C Cuts at: 2244 2244 Size: 6040 ApaLI G'TGCA_C Cuts at: 2018 4097 5253 2018 Size: 2079 1156 2805 Fragments arranged by size: 2805 2079 1156 ApoI r'AATT_y Cuts at: 1493 3349 3575 3727 5836 1493 Size: 1856 226 152 2109 1697 Fragments arranged by size: 2109 1856 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4594 160 Size: 872 3562 1606 Fragments arranged by size: 3562 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6040 AvaI C'yCGr_G Cuts at: 1870 1943 2236 2864 3078 3365 1870 Size: 73 293 628 214 287 4545 Fragments arranged by size: 4545 628 293 287 214 73 BamHI G'GATC_C Cuts at: 3036 3370 3737 3036 Size: 334 367 5339 Fragments arranged by size: 5339 367 334 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2054 2105 2216 2447 2512 Size: 325 217 627 267 51 111 231 65 Cuts at: 2512 3361 4816 618 Size: 849 1455 1842 Fragments arranged by size: 1842 1455 849 627 325 267 231 217 111 65 51 BanII G_rGCy'C Cuts at: 1391 2214 2244 2550 2871 3359 1391 Size: 823 30 306 321 488 4072 Fragments arranged by size: 4072 823 488 321 306 30 BbeI G_GCGC'C Cuts at: 2058 2058 Size: 6040 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5069 BbvCI CC'TCA_GC Cuts at: 3324 3324 Size: 6040 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2032 2066 4231 4265 1903 Size: 34 95 34 2165 34 3678 Fragments arranged by size: 3678 2165 95 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3932 5142 3932 Size: 1210 4830 BclI T'GATC_A Cuts at: 1548 2073 1548 Size: 525 5515 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2248 4651 136 Size: 107 122 71 631 605 576 2403 1525 Fragments arranged by size: 2403 1525 631 605 576 122 107 71 BglII A'GATC_T Cuts at: 2253 6035 2253 Size: 3782 2258 BlpI GC'TnA_GC Cuts at: 3457 3457 Size: 6040 Bme1580I G_kGCm'C Cuts at: 1790 2022 2108 2187 2244 2319 2865 4101 5257 Size: 232 86 79 57 75 546 1236 1156 Cuts at: 5257 1790 Size: 2573 Fragments arranged by size: 2573 1236 1156 546 232 86 79 75 57 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6040 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2314 2442 3771 Size: 416 53 83 399 24 897 128 1329 Cuts at: 3771 4729 442 Size: 958 1753 Fragments arranged by size: 1753 1329 958 897 416 399 128 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6040 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2228 4700 1239 Size: 989 2472 2579 Fragments arranged by size: 2579 2472 989 Bpu10I CC'TnA_GC Cuts at: 3324 3324 Size: 6040 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4165 5030 5328 4165 Size: 865 298 4877 Fragments arranged by size: 4877 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2591 4703 882 Size: 617 1092 2112 2219 Fragments arranged by size: 2219 2112 1092 617 BsaAI yAC'GTr Cuts at: 493 1204 2182 3831 493 Size: 711 978 1649 2702 Fragments arranged by size: 2702 1649 978 711 BsaBI GATnn'nnATC Cuts at: 6034 6034 Size: 6040 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2055 3847 4229 4864 Size: 53 83 186 936 522 1792 382 635 Cuts at: 4864 275 Size: 1451 Fragments arranged by size: 1792 1451 936 635 522 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4473 4871 5145 5292 5703 4473 Size: 398 274 147 411 4810 Fragments arranged by size: 4810 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3293 3323 1178 Size: 30 165 30 1890 30 3895 Fragments arranged by size: 3895 1890 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2444 2489 3311 3314 3329 3332 3341 3344 Size: 1258 45 822 3 15 3 9 3 Cuts at: 3344 1186 Size: 3882 Fragments arranged by size: 3882 1258 822 45 15 9 3 3 3 BseYI C'CCAG_C Cuts at: 2294 2874 5243 5739 2294 Size: 580 2369 496 2595 Fragments arranged by size: 2595 2369 580 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2777 3173 1163 Size: 573 1041 396 4030 Fragments arranged by size: 4030 1041 573 396 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2264 3490 4251 5279 1540 Size: 275 186 263 1226 761 1028 2301 Fragments arranged by size: 2301 1226 1028 761 275 263 186 BsiHKAI G_wGCw'C Cuts at: 2022 2088 2199 2871 4101 4186 5257 2022 Size: 66 111 672 1230 85 1071 2805 Fragments arranged by size: 2805 1230 1071 672 111 85 66 BsmI GAATG_Cn' Cuts at: 3551 3644 3551 Size: 93 5947 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2088 2108 2187 2199 2214 2244 Size: 399 232 66 20 79 12 15 30 Cuts at: 2244 2319 2550 2865 2871 3359 4101 4186 5257 Size: 75 231 315 6 488 742 85 1071 Cuts at: 5257 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 488 399 315 232 231 85 79 75 66 30 20 15 12 6 BspEI T'CCGG_A Cuts at: 5703 5703 Size: 6040 BspHI T'CATG_A Cuts at: 2493 3929 5659 2493 Size: 1436 1730 2874 Fragments arranged by size: 2874 1730 1436 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2136 2726 2760 3395 844 Size: 1292 590 34 635 3489 Fragments arranged by size: 3489 1292 635 590 34 BsrBI CCG'CTC Cuts at: 1394 1823 3927 1394 Size: 429 2104 3507 Fragments arranged by size: 3507 2104 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4535 4709 65 Size: 1183 3287 174 1396 Fragments arranged by size: 3287 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2168 4684 4871 1700 Size: 110 358 2516 187 2869 Fragments arranged by size: 2869 2516 358 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6040 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6040 BssSI C'ACGA_G Cuts at: 2198 4100 5112 2198 Size: 1902 1012 3126 Fragments arranged by size: 3126 1902 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6040 BstBI TT'CG_AA Cuts at: 4844 4844 Size: 6040 BstEII G'GTnAC_C Cuts at: 1184 2672 2710 1184 Size: 1488 38 4514 Fragments arranged by size: 4514 1488 38 BstYI r'GATC_y Cuts at: 1972 2203 2253 2466 3036 3370 3737 4122 4139 Size: 231 50 213 570 334 367 385 17 Cuts at: 4139 5591 5677 5689 6035 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 570 385 367 346 334 231 213 86 50 17 12 BtgI C'CryG_G Cuts at: 513 1065 2100 2113 2220 2923 513 Size: 552 1035 13 107 703 3630 Fragments arranged by size: 3630 1035 703 552 107 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2094 487 Size: 683 56 868 4433 Fragments arranged by size: 4433 868 683 56 BtsI GCAGTG_nn' Cuts at: 2042 3556 4350 4370 2042 Size: 1514 794 20 3712 Fragments arranged by size: 3712 1514 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6040 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3088 3123 529 Size: 35 2524 35 3446 Fragments arranged by size: 3446 2524 35 35 DraI TTT'AAA Cuts at: 3344 3344 Size: 6040 DraIII CAC_nnn'GTG Cuts at: 2689 2689 Size: 6040 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5047 809 Size: 507 3731 1802 Fragments arranged by size: 3731 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2261 2926 3120 Size: 54 1425 50 275 18 431 665 194 Cuts at: 3120 3162 3336 3487 4376 6005 8 Size: 42 174 151 889 1629 43 Fragments arranged by size: 1629 1425 889 665 431 275 194 174 151 54 50 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 2261 3487 1537 Size: 275 449 1226 4090 Fragments arranged by size: 4090 1226 449 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3970 1353 Size: 2617 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3085 4627 5001 5147 387 Size: 1064 138 1496 1542 374 146 1280 Fragments arranged by size: 1542 1496 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2869 2869 Size: 6040 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2228 2623 4103 4700 5487 5755 1239 Size: 393 596 395 1480 597 787 268 1524 Fragments arranged by size: 1524 1480 787 597 596 395 393 268 EcoO109I rG'GnC_Cy Cuts at: 2240 2241 2240 Size: 1 6039 EcoRI G'AATT_C Cuts at: 3349 3727 3349 Size: 378 5662 FspI TGC'GCA Cuts at: 4546 4546 Size: 6040 HaeII r_GCGC'y Cuts at: 1678 2058 2855 5187 1678 Size: 380 797 2332 2531 Fragments arranged by size: 2531 2332 797 380 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3245 3277 4681 4713 4755 Size: 32 135 32 1673 32 1404 32 42 Cuts at: 4755 4787 1373 Size: 32 2626 Fragments arranged by size: 2626 1673 1404 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3384 3630 669 Size: 988 281 1446 246 3079 Fragments arranged by size: 3079 1446 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6040 HpaI GTT'AAC Cuts at: 3630 3630 Size: 6040 KasI G'GCGC_C Cuts at: 2054 2054 Size: 6040 KpnI G_GTAC'C Cuts at: 2451 3365 2451 Size: 914 5126 MfeI C'AATT_G Cuts at: 3639 3639 Size: 6040 MluI A'CGCG_T Cuts at: 5916 5916 Size: 6040 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5154 5338 1305 Size: 3849 184 2007 Fragments arranged by size: 3849 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4218 Fragments arranged by size: 4218 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2147 2540 2969 3164 3998 4357 4516 Size: 648 981 393 429 195 834 359 159 Cuts at: 4516 518 Size: 2042 Fragments arranged by size: 2042 981 834 648 429 393 359 195 159 MspA1I CmG'CkG Cuts at: 1606 2134 2407 3456 4133 5281 5526 1606 Size: 528 273 1049 677 1148 245 2120 Fragments arranged by size: 2120 1148 1049 677 528 273 245 NarI GG'CG_CC Cuts at: 2055 2055 Size: 6040 NcoI C'CATG_G Cuts at: 513 1065 2220 2923 513 Size: 552 1155 703 3630 Fragments arranged by size: 3630 1155 703 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6040 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6040 NotI GC'GGCC_GC Cuts at: 3487 3487 Size: 6040 NspI r_CATG'y Cuts at: 1132 1229 2968 3398 4943 1132 Size: 97 1739 430 1545 2229 Fragments arranged by size: 2229 1739 1545 430 97 PasI CC'CwG_GG Cuts at: 1348 2419 1348 Size: 1071 4969 PciI A'CATG_T Cuts at: 4939 4939 Size: 6040 PflMI CCAn_nnn'nTGG Cuts at: 1108 2064 2419 1108 Size: 956 355 4729 Fragments arranged by size: 4729 956 355 PfoI T'CCnGG_A Cuts at: 2469 3126 2469 Size: 657 5383 PmeI GTTT'AAAC Cuts at: 3344 3344 Size: 6040 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3209 3241 3293 3325 4109 4141 5622 5654 3209 Size: 32 52 32 784 32 1481 32 3595 Fragments arranged by size: 3595 1481 784 52 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6040 PsiI TTA'TAA Cuts at: 3610 3610 Size: 6040 PspOMI G'GGCC_C Cuts at: 2240 2240 Size: 6040 PstI C_TGCA'G Cuts at: 830 2133 2397 3392 830 Size: 1303 264 995 3478 Fragments arranged by size: 3478 1303 995 264 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6040 PvuII CAG'CTG Cuts at: 2134 2407 2134 Size: 273 5767 SacI G_AGCT'C Cuts at: 2871 2871 Size: 6040 SalI G'TCGA_C Cuts at: 1936 3382 1936 Size: 1446 4594 SbfI CC_TGCA'GG Cuts at: 2397 3392 2397 Size: 995 5045 ScaI AGT'ACT Cuts at: 4288 6000 4288 Size: 1712 4328 SexAI A'CCwGG_T Cuts at: 2685 3255 2685 Size: 570 5470 SfcI C'TryA_G Cuts at: 826 1045 2129 2393 3388 4523 5204 5395 826 Size: 219 1084 264 995 1135 681 191 1471 Fragments arranged by size: 1471 1135 1084 995 681 264 219 191 SfoI GGC'GCC Cuts at: 2056 2056 Size: 6040 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6040 SgrAI Cr'CCGG_yG Cuts at: 1700 2168 1700 Size: 468 5572 SmaI CCC'GGG Cuts at: 1872 3367 1872 Size: 1495 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4144 5045 5307 820 Size: 197 926 2201 901 262 1553 Fragments arranged by size: 2201 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3831 493 Size: 3338 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6040 SphI G_CATG'C Cuts at: 1132 3398 1132 Size: 2266 3774 SspI AAT'ATT Cuts at: 5 52 3964 5 Size: 47 3912 2081 Fragments arranged by size: 3912 2081 47 StuI AGG'CCT Cuts at: 1517 3182 1517 Size: 1665 4375 StyI C'CwwG_G Cuts at: 513 1065 2220 2310 2923 3479 513 Size: 552 1155 90 613 556 3074 Fragments arranged by size: 3074 1155 613 556 552 90 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2270 2411 2888 4067 4084 4237 4422 Size: 1255 690 141 477 1179 17 153 185 Cuts at: 4422 4867 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1179 690 477 445 185 153 141 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2151 4286 5998 Size: 274 80 33 51 1443 174 2135 1712 Cuts at: 5998 96 Size: 138 Fragments arranged by size: 2135 1712 1443 274 174 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6000 6032 6000 Size: 32 6008 Tth111I GACn'n_nGTC Cuts at: 1316 2434 1316 Size: 1118 4922 XbaI T'CTAG_A Cuts at: 3376 3376 Size: 6040 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2420 2899 2977 2420 Size: 479 78 5483 Fragments arranged by size: 5483 479 78 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6040 XmaI C'CCGG_G Cuts at: 1870 3365 1870 Size: 1495 4545 XmnI GAAnn'nnTTC Cuts at: 4169 4169 Size: 6040 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3848 276 Size: 53 83 186 936 2314 2468 Fragments arranged by size: 2468 2314 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI