Restriction map for
pFN21AE2448
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AccIII AclI AflII AflIII AgeI
AhdI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I AspEI AspI
AvaI AviII AvrII BamHI BbrPI
BcgI BciVI BfrI BlnI BsaAI
BsaBI BsaWI BseAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI Eam1105I
EarI Eco47III Eco81I EcoNI EcoRI
EcoRV EcoT14I EcoT22I FspI HindIII
HpaI Ksp632I KspI MamI MfeI
MluI MroI MunI NcoI NdeI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI ScaI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SspI StyI
SwaI TaqI Tth111I TthHB8I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 26 enzymes) #
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AatII 271 GACGTC GACGT^C
Acc65I 1382 GGTACC G^GTACC
AccI 1468 GTCTAC GT^[AC][GT]AC
AcsI 550 GAATTT [GA]^AATT[CT]
AlwI 587 GGATC GGATC(4/5)
ApoI 550 GAATTT [GA]^AATT[CT]
Asp718I 1382 GGTACC G^GTACC
BbsI 1180 GTCTTC GAAGAC(2/6)
BglII 1775 AGATCT A^GATCT
BpuAI 1180 GTCTTC GAAGAC(2/6)
BsaI 519 GGTCTC GGTCTC(1/5)
BspHI 773 TCATGA T^CATGA
BsrBI 733 CCGCTC CCGCTC(-3/-3)
BstAPI 1715 GCAACGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
FseI 1613 GGCCGGCC GGCCGG^CC
KpnI 1382 GGTACC GGTAC^C
MseI 815 TTAA T^TAA
NheI 663 GCTAGC G^CTAGC
NotI 1052 GCGGCCGC GC^GGCCGC
RcaI 773 TCATGA T^CATGA
SbfI 1329 CCTGCAGG CCTGCA^GG
SexAI 1385 ACCTGGT A^CC[AT]GGT
Sse8387I 1329 CCTGCAGG CCTGCA^GG
TfiI 1484 GATTC G^A[AT]TC
Tru9I 815 TTAA T^TAA
Tsp509I 551 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
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Alw44I 580 GTGCAC G^TGCAC
882 GTGCAC
AlwNI 292 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
858 CAGCAGCTG
ApaLI 580 GTGCAC G^TGCAC
882 GTGCAC
BalI 1298 TGGCCA TGG^CCA
1418 TGGCCA
BbvCI 142 CCTCAGC CCTCAGC(-5/-2)
276 CCTCAGC
BclI 770 TGATCA T^GATCA
1220 TGATCA
BfaI 58 CTAG C^TAG
664 CTAG
BglI 1282 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1614 GCCGGCCTGGC
BlpI 442 GCTAAGC GC^T[ACGT]AGC
1320 GCTCAGC
Bpu1102I 442 GCTAAGC GC^T[ACGT]AGC
1320 GCTCAGC
BspMI 1472 ACCTGC ACCTGC(4/8)
1332 GCAGGT
BstEII 1437 GGTGACC G^GT[ACGT]ACC
1695 GGTGACC
BstPI 1437 GGTGACC G^GT[ACGT]ACC
1695 GGTGACC
BstYI 587 GGATCT [GA]^GATC[CT]
1775 AGATCT
CelII 442 GCTAAGC GC^T[ACGT]AGC
1320 GCTCAGC
EcoO65I 1437 GGTGACC G^GT[ACGT]ACC
1695 GGTGACC
FbaI 770 TGATCA T^GATCA
1220 TGATCA
HincII 52 GTTGAC GT[CT]^[GA]AC
487 GTCAAC
HindII 52 GTTGAC GT[CT]^[GA]AC
487 GTCAAC
MaeI 58 CTAG C^TAG
664 CTAG
MflI 587 GGATCT [GA]^GATC[CT]
1775 AGATCT
MluNI 1298 TGGCCA TGG^CCA
1418 TGGCCA
MscI 1298 TGGCCA TGG^CCA
1418 TGGCCA
PflMI 291 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1668 CCATTCTCTGG
PleI 69 GACTC GAGTC(4/5)
760 GACTC
SacI 175 GAGCTC GAGCT^C
949 GAGCTC
SmlI 220 CTCAAG C^T[CT][GA]AG
715 CTCAAG
StuI 224 AGGCCT AGG^CCT
1751 AGGCCT
Van91I 291 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1668 CCATTCTCTGG
XcmI 635 CCACGGCCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1277 CCACAGCCCTGCTGG
XhoII 587 GGATCT [GA]^GATC[CT]
1775 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaII BanI BanII BbeI
BbvI BcnI BmyI BpmI BsaHI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EclXI Eco52I EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI TseI
Tsp45I TspRI