(Circular) (Six-base) MAPSORT of: pFN21AE2448.seq Check: 1502 from: 1 to: 6502 pFN21AE2448 6502 bp With 182 enzymes: SgfI * January 7, 2013 17:02 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2280 4312 278 Size: 53 83 186 936 744 2032 2468 Fragments arranged by size: 2468 2032 936 744 186 83 53 AccI GT'mk_AC Cuts at: 1937 3474 3845 1937 Size: 1537 371 4594 Fragments arranged by size: 4594 1537 371 Acc65I G'GTAC_C Cuts at: 3387 3823 3387 Size: 436 6066 AclI AA'CG_TT Cuts at: 1563 4629 5002 1563 Size: 3066 373 3063 Fragments arranged by size: 3066 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2174 4565 5949 6217 1632 Size: 542 2391 1384 268 1917 Fragments arranged by size: 2391 1917 1384 542 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6305 AflIII A'CryG_T Cuts at: 1203 5401 6378 1203 Size: 4198 977 1327 Fragments arranged by size: 4198 1327 977 AgeI A'CCGG_T Cuts at: 5333 5333 Size: 6502 AhdI GACnn_n'nnGTC Cuts at: 5231 5231 Size: 6502 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6502 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6502 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2083 2115 2083 Size: 32 6470 AlwNI CAG_nnn'CTG Cuts at: 2302 2868 4245 5817 2302 Size: 566 1377 1572 2987 Fragments arranged by size: 2987 1572 1377 566 ApaLI G'TGCA_C Cuts at: 2585 2887 4559 5715 2585 Size: 302 1672 1156 3372 Fragments arranged by size: 3372 1672 1156 302 ApoI r'AATT_y Cuts at: 1493 2555 3811 4037 4189 6298 1493 Size: 1062 1256 226 152 2109 1697 Fragments arranged by size: 2109 1697 1256 1062 226 152 AseI AT'TA_AT Cuts at: 160 1032 5056 160 Size: 872 4024 1606 Fragments arranged by size: 4024 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6502 AvaI C'yCGr_G Cuts at: 1870 1943 3827 1870 Size: 73 1884 4545 Fragments arranged by size: 4545 1884 73 BamHI G'GATC_C Cuts at: 3832 4199 3832 Size: 367 6135 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2678 2707 2834 2873 3149 Size: 325 217 627 891 29 127 39 276 Cuts at: 3149 3272 3387 3394 3612 3823 5278 618 Size: 123 115 7 218 211 1455 1842 Fragments arranged by size: 1842 1455 891 627 325 276 218 217 211 127 123 115 39 29 7 BanII G_rGCy'C Cuts at: 1391 2184 2241 2958 3263 3522 3687 3821 1391 Size: 793 57 717 305 259 165 134 4072 Fragments arranged by size: 4072 793 717 305 259 165 134 57 BbeI G_GCGC'C Cuts at: 2711 2877 3153 3616 2711 Size: 166 276 463 5597 Fragments arranged by size: 5597 463 276 166 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3178 928 Size: 971 1279 4252 Fragments arranged by size: 4252 1279 971 BbvCI CC'TCA_GC Cuts at: 2148 2282 2148 Size: 134 6368 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4693 4727 1903 Size: 34 50 34 2672 34 3678 Fragments arranged by size: 3678 2672 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4394 5604 4394 Size: 1210 5292 BclI T'GATC_A Cuts at: 1548 2775 3225 1548 Size: 1227 450 4825 Fragments arranged by size: 4825 1227 450 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3293 3625 5113 Size: 107 122 71 631 605 1621 332 1488 Cuts at: 5113 136 Size: 1525 Fragments arranged by size: 1621 1525 1488 631 605 332 122 107 71 BglII A'GATC_T Cuts at: 3780 6497 3780 Size: 2717 3785 BlpI GC'TnA_GC Cuts at: 2448 3326 3919 2448 Size: 878 593 5031 Fragments arranged by size: 5031 878 593 Bme1580I G_kGCm'C Cuts at: 1790 2478 2589 2891 2951 3207 4563 5719 1790 Size: 688 111 302 60 256 1356 1156 2573 Fragments arranged by size: 2573 1356 1156 688 302 256 111 60 BmgBI CAC'GTC Cuts at: 1318 2415 1318 Size: 1097 5405 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3266 4233 5191 Size: 416 53 83 399 24 1849 967 958 Cuts at: 5191 442 Size: 1753 Fragments arranged by size: 1849 1753 967 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2672 1056 Size: 1616 4886 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2080 2091 2651 2672 3701 5162 1239 Size: 841 11 560 21 1029 1461 2579 Fragments arranged by size: 2579 1461 1029 841 560 21 11 Bpu10I CC'TnA_GC Cuts at: 2148 2282 2148 Size: 134 6368 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2210 2705 4627 5492 5790 2210 Size: 495 1922 865 298 2922 Fragments arranged by size: 2922 1922 865 495 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2530 5165 882 Size: 617 1031 2635 2219 Fragments arranged by size: 2635 2219 1031 617 BsaAI yAC'GTr Cuts at: 493 1204 4293 493 Size: 711 3089 2702 Fragments arranged by size: 3089 2702 711 BsaBI GATnn'nnATC Cuts at: 6496 6496 Size: 6502 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2277 2708 2874 3150 Size: 53 83 186 936 744 431 166 276 Cuts at: 3150 3613 4309 4691 5326 275 Size: 463 696 382 635 1451 Fragments arranged by size: 1451 936 744 696 635 463 431 382 276 186 166 83 53 BsaWI w'CCGG_w Cuts at: 4935 5333 5607 5754 6165 4935 Size: 398 274 147 411 5272 Fragments arranged by size: 5272 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6277 Fragments arranged by size: 6277 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2231 2410 3572 1186 Size: 1045 179 1162 4116 Fragments arranged by size: 4116 1162 1045 179 BseYI C'CCAG_C Cuts at: 2302 3112 3459 3467 3792 5705 6201 2302 Size: 810 347 8 325 1913 496 2603 Fragments arranged by size: 2603 1913 810 496 347 325 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2420 2777 3384 1163 Size: 573 684 357 607 4281 Fragments arranged by size: 4281 684 607 573 357 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3061 3198 3620 3952 4713 5741 Size: 275 186 1060 137 422 332 761 1028 Cuts at: 5741 1540 Size: 2301 Fragments arranged by size: 2301 1060 1028 761 422 332 275 186 137 BsiHKAI G_wGCw'C Cuts at: 2184 2589 2891 2958 3009 3582 4563 4648 5719 Size: 405 302 67 51 573 981 85 1071 Cuts at: 5719 2184 Size: 2967 Fragments arranged by size: 2967 1071 981 573 405 302 85 67 51 BsmI GAATG_Cn' Cuts at: 4013 4106 4013 Size: 93 6409 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2184 2241 2478 2589 2891 2951 2958 Size: 399 394 57 237 111 302 60 7 Cuts at: 2958 3009 3207 3263 3522 3582 3687 3821 4563 Size: 51 198 56 259 60 105 134 742 Cuts at: 4563 4648 5719 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 394 302 259 237 198 134 111 105 85 60 60 57 56 51 7 BspEI T'CCGG_A Cuts at: 6165 6165 Size: 6502 BspHI T'CATG_A Cuts at: 2778 4391 6121 2778 Size: 1613 1730 3159 Fragments arranged by size: 3159 1730 1613 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3328 3486 3857 844 Size: 2484 158 371 3489 Fragments arranged by size: 3489 2484 371 158 BsrBI CCG'CTC Cuts at: 1394 1823 2740 4389 1394 Size: 429 917 1649 3507 Fragments arranged by size: 3507 1649 917 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4997 5171 65 Size: 1183 3749 174 1396 Fragments arranged by size: 3749 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2192 2213 2990 3152 3371 3615 3619 Size: 110 382 21 777 162 219 244 4 Cuts at: 3619 5146 5333 1700 Size: 1527 187 2869 Fragments arranged by size: 2869 1527 777 382 244 219 187 162 110 21 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6502 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6502 BssSI C'ACGA_G Cuts at: 4562 5574 4562 Size: 1012 5490 BstAPI GCAn_nnn'nTGC Cuts at: 794 3726 794 Size: 2932 3570 BstBI TT'CG_AA Cuts at: 5306 5306 Size: 6502 BstEII G'GTnAC_C Cuts at: 1184 3442 3700 1184 Size: 2258 258 3986 Fragments arranged by size: 3986 2258 258 BstXI CCAn_nnnn'nTGG Cuts at: 2359 2485 2521 3421 2359 Size: 126 36 900 5440 Fragments arranged by size: 5440 900 126 36 BstYI r'GATC_y Cuts at: 1972 2592 3780 3832 4199 4584 4601 6053 6139 Size: 620 1188 52 367 385 17 1452 86 Cuts at: 6139 6151 6497 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1188 620 385 367 346 86 52 17 12 BtgI C'CryG_G Cuts at: 513 1065 2640 2838 2970 513 Size: 552 1575 198 132 4045 Fragments arranged by size: 4045 1575 552 198 132 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5763 Fragments arranged by size: 5763 683 56 BtsI GCAGTG_nn' Cuts at: 2791 3167 3401 4018 4812 4832 2791 Size: 376 234 617 794 20 4461 Fragments arranged by size: 4461 794 617 376 234 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6502 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6467 DraI TTT'AAA Cuts at: 3806 3806 Size: 6502 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5509 809 Size: 507 4193 1802 Fragments arranged by size: 4193 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2190 2211 2316 Size: 54 1425 50 275 18 360 21 105 Cuts at: 2316 2967 2988 3058 3135 3195 3303 3369 3423 Size: 651 21 70 77 60 108 66 54 Cuts at: 3423 3546 3617 3949 4838 6467 8 Size: 123 71 332 889 1629 43 Fragments arranged by size: 1629 1425 889 651 360 332 275 123 108 105 77 71 70 66 60 54 54 50 43 21 21 18 EagI C'GGCC_G Cuts at: 1537 1812 3058 3195 3617 3949 1537 Size: 275 1246 137 422 332 4090 Fragments arranged by size: 4090 1246 422 332 275 137 EarI CTCTTCn'nnn_ Cuts at: 1353 4432 1353 Size: 3079 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2278 2930 3626 5089 5463 5609 Size: 1064 138 689 652 696 1463 374 146 Cuts at: 5609 387 Size: 1280 Fragments arranged by size: 1463 1280 1064 696 689 652 374 146 138 EcoICRI GAG'CTC Cuts at: 2182 2956 2182 Size: 774 5728 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2080 2091 2174 2651 2672 3701 4565 Size: 393 448 11 83 477 21 1029 864 Cuts at: 4565 5162 5949 6217 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1029 864 787 597 477 448 393 268 83 21 11 EcoO109I rG'GnC_Cy Cuts at: 2032 2091 3560 2032 Size: 59 1469 4974 Fragments arranged by size: 4974 1469 59 EcoRI G'AATT_C Cuts at: 3811 4189 3811 Size: 378 6124 FseI GG_CCGG'CC Cuts at: 3623 3623 Size: 6502 FspI TGC'GCA Cuts at: 5008 5008 Size: 6502 HaeII r_GCGC'y Cuts at: 1678 2640 2711 2877 3153 3333 3616 5649 1678 Size: 962 71 166 276 180 283 2033 2531 Fragments arranged by size: 2531 2033 962 283 276 180 166 71 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2131 2163 5143 5175 5217 Size: 32 135 32 559 32 2980 32 42 Cuts at: 5217 5249 1373 Size: 32 2626 Fragments arranged by size: 2980 2626 559 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2059 2494 3846 4092 669 Size: 988 281 121 435 1352 246 3079 Fragments arranged by size: 3079 1352 988 435 281 246 121 HindIII A'AGCT_T Cuts at: 748 748 Size: 6502 HpaI GTT'AAC Cuts at: 4092 4092 Size: 6502 KasI G'GCGC_C Cuts at: 2707 2873 3149 3612 2707 Size: 166 276 463 5597 Fragments arranged by size: 5597 463 276 166 KpnI G_GTAC'C Cuts at: 3391 3827 3391 Size: 436 6066 MfeI C'AATT_G Cuts at: 4101 4101 Size: 6502 MluI A'CGCG_T Cuts at: 6378 6378 Size: 6502 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2376 2742 2793 3288 5616 5800 1305 Size: 1071 366 51 495 2328 184 2007 Fragments arranged by size: 2328 2007 1071 495 366 184 51 MscI TGG'CCA Cuts at: 10 64 1489 1832 3305 3425 10 Size: 54 1425 343 1473 120 3087 Fragments arranged by size: 3087 1473 1425 343 120 54 MslI CAynn'nnrTG Cuts at: 518 1166 2378 2483 2777 4460 4819 4978 518 Size: 648 1212 105 294 1683 359 159 2042 Fragments arranged by size: 2042 1683 1212 648 359 294 159 105 MspA1I CmG'CkG Cuts at: 1606 2152 2299 2574 2868 3112 3421 3459 3551 Size: 546 147 275 294 244 309 38 92 Cuts at: 3551 3610 3708 3918 4595 5743 5988 1606 Size: 59 98 210 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 546 309 294 275 245 244 210 147 98 92 59 38 NaeI GCC'GGC Cuts at: 2194 2215 2992 3154 3373 3617 3621 2194 Size: 21 777 162 219 244 4 5075 Fragments arranged by size: 5075 777 244 219 162 21 4 NarI GG'CG_CC Cuts at: 2708 2874 3150 3613 2708 Size: 166 276 463 5597 Fragments arranged by size: 5597 463 276 166 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5950 NdeI CA'TA_TG Cuts at: 387 387 Size: 6502 NgoMIV G'CCGG_C Cuts at: 2192 2213 2990 3152 3371 3615 3619 2192 Size: 21 777 162 219 244 4 5075 Fragments arranged by size: 5075 777 244 219 162 21 4 NheI G'CTAG_C Cuts at: 1052 2668 1052 Size: 1616 4886 NotI GC'GGCC_GC Cuts at: 3058 3949 3058 Size: 891 5611 NspI r_CATG'y Cuts at: 1132 1229 3860 5405 1132 Size: 97 2631 1545 2229 Fragments arranged by size: 2631 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2674 2697 3687 1348 Size: 1326 23 990 4163 Fragments arranged by size: 4163 1326 990 23 PciI A'CATG_T Cuts at: 5401 5401 Size: 6502 PflMI CCAn_nnn'nTGG Cuts at: 1108 2302 3679 3799 1108 Size: 1194 1377 120 3811 Fragments arranged by size: 3811 1377 1194 120 PmeI GTTT'AAAC Cuts at: 3806 3806 Size: 6502 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4571 4603 6084 6116 4571 Size: 32 1481 32 4957 Fragments arranged by size: 4957 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2032 2091 3560 2032 Size: 59 1469 4974 Fragments arranged by size: 4974 1469 59 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6502 PsiI TTA'TAA Cuts at: 4072 4072 Size: 6502 PstI C_TGCA'G Cuts at: 830 3174 3339 3609 3731 3854 830 Size: 2344 165 270 122 123 3478 Fragments arranged by size: 3478 2344 270 165 123 122 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6502 PvuII CAG'CTG Cuts at: 2152 2299 2868 2152 Size: 147 569 5786 Fragments arranged by size: 5786 569 147 SacI G_AGCT'C Cuts at: 2184 2958 2184 Size: 774 5728 SalI G'TCGA_C Cuts at: 1936 3844 1936 Size: 1908 4594 SanDI GG'GwC_CC Cuts at: 3560 3560 Size: 6502 SbfI CC_TGCA'GG Cuts at: 3339 3854 3339 Size: 515 5987 ScaI AGT'ACT Cuts at: 4750 6462 4750 Size: 1712 4790 SexAI A'CCwGG_T Cuts at: 3390 3390 Size: 6502 SfcI C'TryA_G Cuts at: 826 1045 3170 3335 3605 3727 3850 4985 5666 Size: 219 2125 165 270 122 123 1135 681 Cuts at: 5666 5857 826 Size: 191 1471 Fragments arranged by size: 2125 1471 1135 681 270 219 191 165 123 122 SfoI GGC'GCC Cuts at: 2709 2875 3151 3614 2709 Size: 166 276 463 5597 Fragments arranged by size: 5597 463 276 166 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6502 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6502 SmaI CCC'GGG Cuts at: 1872 3829 1872 Size: 1957 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2225 2720 4606 5507 5769 820 Size: 197 926 282 495 1886 901 262 1553 Fragments arranged by size: 1886 1553 926 901 495 282 262 197 SnaBI TAC'GTA Cuts at: 493 4293 493 Size: 3800 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6502 SphI G_CATG'C Cuts at: 1132 3860 1132 Size: 2728 3774 SspI AAT'ATT Cuts at: 5 52 4426 5 Size: 47 4374 2081 Fragments arranged by size: 4374 2081 47 StuI AGG'CCT Cuts at: 1517 2231 3758 1517 Size: 714 1527 4261 Fragments arranged by size: 4261 1527 714 StyI C'CwwG_G Cuts at: 513 1065 3941 513 Size: 552 2876 3074 Fragments arranged by size: 3074 2876 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2477 2666 2710 3451 4529 4546 4699 Size: 1255 897 189 44 741 1078 17 153 Cuts at: 4699 4884 5329 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1078 897 741 445 189 185 153 44 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2253 4748 6460 Size: 274 80 33 51 1443 276 2495 1712 Cuts at: 6460 96 Size: 138 Fragments arranged by size: 2495 1712 1443 276 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6462 6494 6462 Size: 32 6470 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6502 XbaI T'CTAG_A Cuts at: 3838 3838 Size: 6502 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2647 3289 2647 Size: 642 5860 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6502 XmaI C'CCGG_G Cuts at: 1870 3827 1870 Size: 1957 4545 XmnI GAAnn'nnTTC Cuts at: 4631 4631 Size: 6502 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2278 4310 276 Size: 53 83 186 936 744 2032 2468 Fragments arranged by size: 2468 2032 936 744 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SapI SfiI SrfI SwaI