(Linear) (Six-base) MAPSORT of: KIEE2448.seq Check: 293 from: 1 to: 1797 KIEE2448.orf With 182 enzymes: SgfI * January 7, 2013 14:39 .. AatII G_ACGT'C Cuts at: 0 275 1797 Size: 275 1522 AccI GT'mk_AC Cuts at: 0 1469 1797 Size: 1469 328 Acc65I G'GTAC_C Cuts at: 0 1382 1797 Size: 1382 415 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 169 1797 Size: 169 1628 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 78 110 1797 Size: 78 32 1687 Fragments arranged by size: 1687 78 32 AlwNI CAG_nnn'CTG Cuts at: 0 297 863 1797 Size: 297 566 934 Fragments arranged by size: 934 566 297 ApaLI G'TGCA_C Cuts at: 0 580 882 1797 Size: 580 302 915 Fragments arranged by size: 915 580 302 ApoI r'AATT_y Cuts at: 0 550 1797 Size: 550 1247 BanI G'GyrC_C Cuts at: 0 673 702 829 868 1144 1267 1382 1389 Size: 673 29 127 39 276 123 115 7 Cuts at: 1389 1607 1797 Size: 218 190 Fragments arranged by size: 673 276 218 190 127 123 115 39 29 7 BanII G_rGCy'C Cuts at: 0 179 236 953 1258 1517 1682 1797 Size: 179 57 717 305 259 165 115 Fragments arranged by size: 717 305 259 179 165 115 57 BbeI G_GCGC'C Cuts at: 0 706 872 1148 1611 1797 Size: 706 166 276 463 186 Fragments arranged by size: 706 463 276 186 166 BbsI GAAGACnn'nnnn_ Cuts at: 0 1173 1797 Size: 1173 624 BbvCI CC'TCA_GC Cuts at: 0 143 277 1797 Size: 143 134 1520 Fragments arranged by size: 1520 143 134 BclI T'GATC_A Cuts at: 0 770 1220 1797 Size: 770 450 577 Fragments arranged by size: 770 577 450 BglI GCCn_nnn'nGGC Cuts at: 0 1288 1620 1797 Size: 1288 332 177 Fragments arranged by size: 1288 332 177 BglII A'GATC_T Cuts at: 0 1775 1797 Size: 1775 22 BlpI GC'TnA_GC Cuts at: 0 443 1321 1797 Size: 443 878 476 Fragments arranged by size: 878 476 443 Bme1580I G_kGCm'C Cuts at: 0 473 584 886 946 1202 1797 Size: 473 111 302 60 256 595 Fragments arranged by size: 595 473 302 256 111 60 BmgBI CAC'GTC Cuts at: 0 410 1797 Size: 410 1387 BmrI ACTGGGnnnn_n' Cuts at: 0 1261 1797 Size: 1261 536 BmtI G_CTAG'C Cuts at: 0 667 1797 Size: 667 1130 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 75 86 646 667 1696 1797 Size: 75 11 560 21 1029 101 Fragments arranged by size: 1029 560 101 75 21 11 Bpu10I CC'TnA_GC Cuts at: 0 143 277 1797 Size: 143 134 1520 Fragments arranged by size: 1520 143 134 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 205 700 1797 Size: 205 495 1097 Fragments arranged by size: 1097 495 205 BsaI GGTCTCn'nnnn_ Cuts at: 0 525 1797 Size: 525 1272 BsaHI Gr'CG_yC Cuts at: 0 272 703 869 1145 1608 1797 Size: 272 431 166 276 463 189 Fragments arranged by size: 463 431 276 272 189 166 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 226 405 1567 1797 Size: 226 179 1162 230 Fragments arranged by size: 1162 230 226 179 BseYI C'CCAG_C Cuts at: 0 297 1107 1454 1462 1787 1797 Size: 297 810 347 8 325 10 Fragments arranged by size: 810 347 325 297 10 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 415 772 1379 1797 Size: 415 357 607 418 Fragments arranged by size: 607 418 415 357 BsiEI CG_ry'CG Cuts at: 0 1056 1193 1615 1797 Size: 1056 137 422 182 Fragments arranged by size: 1056 422 182 137 BsiHKAI G_wGCw'C Cuts at: 0 179 584 886 953 1004 1577 1797 Size: 179 405 302 67 51 573 220 Fragments arranged by size: 573 405 302 220 179 67 51 Bsp1286I G_dGCh'C Cuts at: 0 179 236 473 584 886 946 953 1004 Size: 179 57 237 111 302 60 7 51 Cuts at: 1004 1202 1258 1517 1577 1682 1797 Size: 198 56 259 60 105 115 Fragments arranged by size: 302 259 237 198 179 115 111 105 60 60 57 56 51 7 BspHI T'CATG_A Cuts at: 0 773 1797 Size: 773 1024 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1323 1481 1797 Size: 1323 158 316 Fragments arranged by size: 1323 316 158 BsrBI CCG'CTC Cuts at: 0 735 1797 Size: 735 1062 BsrFI r'CCGG_y Cuts at: 0 187 208 985 1147 1366 1610 1614 1797 Size: 187 21 777 162 219 244 4 183 Fragments arranged by size: 777 244 219 187 183 162 21 4 BstAPI GCAn_nnn'nTGC Cuts at: 0 1721 1797 Size: 1721 76 BstEII G'GTnAC_C Cuts at: 0 1437 1695 1797 Size: 1437 258 102 Fragments arranged by size: 1437 258 102 BstXI CCAn_nnnn'nTGG Cuts at: 0 354 480 516 1416 1797 Size: 354 126 36 900 381 Fragments arranged by size: 900 381 354 126 36 BstYI r'GATC_y Cuts at: 0 587 1775 1797 Size: 587 1188 22 Fragments arranged by size: 1188 587 22 BtgI C'CryG_G Cuts at: 0 635 833 965 1797 Size: 635 198 132 832 Fragments arranged by size: 832 635 198 132 BtsI GCAGTG_nn' Cuts at: 0 786 1162 1396 1797 Size: 786 376 234 401 Fragments arranged by size: 786 401 376 234 EaeI y'GGCC_r Cuts at: 0 185 206 311 962 983 1053 1130 1190 Size: 185 21 105 651 21 70 77 60 Cuts at: 1190 1298 1364 1418 1541 1612 1797 Size: 108 66 54 123 71 185 Fragments arranged by size: 651 185 185 123 108 105 77 71 70 66 60 54 21 21 EagI C'GGCC_G Cuts at: 0 1053 1190 1612 1797 Size: 1053 137 422 185 Fragments arranged by size: 1053 422 185 137 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 273 925 1621 1797 Size: 273 652 696 176 Fragments arranged by size: 696 652 273 176 EcoICRI GAG'CTC Cuts at: 0 177 951 1797 Size: 177 774 846 Fragments arranged by size: 846 774 177 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 75 86 169 646 667 1696 1797 Size: 75 11 83 477 21 1029 101 Fragments arranged by size: 1029 477 101 83 75 21 11 EcoO109I rG'GnC_Cy Cuts at: 0 27 86 1555 1797 Size: 27 59 1469 242 Fragments arranged by size: 1469 242 59 27 FseI GG_CCGG'CC Cuts at: 0 1618 1797 Size: 1618 179 HaeII r_GCGC'y Cuts at: 0 635 706 872 1148 1328 1611 1797 Size: 635 71 166 276 180 283 186 Fragments arranged by size: 635 283 276 186 180 166 71 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 126 158 1797 Size: 126 32 1639 Fragments arranged by size: 1639 126 32 HincII GTy'rAC Cuts at: 0 54 489 1797 Size: 54 435 1308 Fragments arranged by size: 1308 435 54 KasI G'GCGC_C Cuts at: 0 702 868 1144 1607 1797 Size: 702 166 276 463 190 Fragments arranged by size: 702 463 276 190 166 KpnI G_GTAC'C Cuts at: 0 1386 1797 Size: 1386 411 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 371 737 788 1283 1797 Size: 371 366 51 495 514 Fragments arranged by size: 514 495 371 366 51 MscI TGG'CCA Cuts at: 0 1300 1420 1797 Size: 1300 120 377 Fragments arranged by size: 1300 377 120 MslI CAynn'nnrTG Cuts at: 0 373 478 772 1797 Size: 373 105 294 1025 Fragments arranged by size: 1025 373 294 105 MspA1I CmG'CkG Cuts at: 0 147 294 569 863 1107 1416 1454 1546 Size: 147 147 275 294 244 309 38 92 Cuts at: 1546 1605 1703 1797 Size: 59 98 94 Fragments arranged by size: 309 294 275 244 147 147 98 94 92 59 38 NaeI GCC'GGC Cuts at: 0 189 210 987 1149 1368 1612 1616 1797 Size: 189 21 777 162 219 244 4 181 Fragments arranged by size: 777 244 219 189 181 162 21 4 NarI GG'CG_CC Cuts at: 0 703 869 1145 1608 1797 Size: 703 166 276 463 189 Fragments arranged by size: 703 463 276 189 166 NgoMIV G'CCGG_C Cuts at: 0 187 208 985 1147 1366 1610 1614 1797 Size: 187 21 777 162 219 244 4 183 Fragments arranged by size: 777 244 219 187 183 162 21 4 NheI G'CTAG_C Cuts at: 0 663 1797 Size: 663 1134 NotI GC'GGCC_GC Cuts at: 0 1053 1797 Size: 1053 744 PasI CC'CwG_GG Cuts at: 0 669 692 1682 1797 Size: 669 23 990 115 Fragments arranged by size: 990 669 115 23 PflMI CCAn_nnn'nTGG Cuts at: 0 297 1674 1797 Size: 297 1377 123 Fragments arranged by size: 1377 297 123 PpuMI rG'GwC_Cy Cuts at: 0 27 86 1555 1797 Size: 27 59 1469 242 Fragments arranged by size: 1469 242 59 27 PstI C_TGCA'G Cuts at: 0 1169 1334 1604 1726 1797 Size: 1169 165 270 122 71 Fragments arranged by size: 1169 270 165 122 71 PvuII CAG'CTG Cuts at: 0 147 294 863 1797 Size: 147 147 569 934 Fragments arranged by size: 934 569 147 147 SacI G_AGCT'C Cuts at: 0 179 953 1797 Size: 179 774 844 Fragments arranged by size: 844 774 179 SanDI GG'GwC_CC Cuts at: 0 1555 1797 Size: 1555 242 SbfI CC_TGCA'GG Cuts at: 0 1334 1797 Size: 1334 463 SexAI A'CCwGG_T Cuts at: 0 1385 1797 Size: 1385 412 SfcI C'TryA_G Cuts at: 0 1165 1330 1600 1722 1797 Size: 1165 165 270 122 75 Fragments arranged by size: 1165 270 165 122 75 SfoI GGC'GCC Cuts at: 0 704 870 1146 1609 1797 Size: 704 166 276 463 188 Fragments arranged by size: 704 463 276 188 166 SmlI C'TyrA_G Cuts at: 0 220 715 1797 Size: 220 495 1082 Fragments arranged by size: 1082 495 220 StuI AGG'CCT Cuts at: 0 226 1753 1797 Size: 226 1527 44 Fragments arranged by size: 1527 226 44 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 472 661 705 1446 1797 Size: 472 189 44 741 351 Fragments arranged by size: 741 472 351 189 44 TatI w'GTAC_w Cuts at: 0 248 1797 Size: 248 1549 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 642 1284 1797 Size: 642 642 513 Fragments arranged by size: 642 642 513 ZraI GAC'GTC Cuts at: 0 273 1797 Size: 273 1524 Enzymes that do cut: AatII AccI Acc65I AcuI AloI AlwNI ApaLI ApoI BanI BanII BbeI BbsI BbvCI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BstAPI BstEII BstXI BstYI BtgI BtsI EaeI EagI EciI EcoICRI Eco57MI EcoO109I FseI HaeII Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI NotI PasI PflMI PpuMI PstI PvuII SacI SanDI SbfI SexAI SfcI SfoI SmlI StuI TaqII TatI XcmI ZraI Enzymes that do not cut: AarI AclI AfeI AflII AflIII AgeI AhdI AleI AlfI ApaI AscI AseI AsiSI AvaI AvrII BaeI BamHI BcgI BciVI BfrBI BplI BsaAI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI BsrDI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EarI EcoNI EcoRI EcoRV FalI FspI FspAI HindIII HpaI MfeI MluI NcoI NdeI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SapI ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StyI SwaI TstI Tth111I XbaI XhoI XmaI XmnI