################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AhdI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanII BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaWI BseAI BsiEI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BsrI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HindIII HpaI KpnI MaeI MamI MfeI MluI MluNI MroI MscI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccI 547 GTCGAC GT^[AC][GT]AC Alw44I 697 GTGCAC G^TGCAC AlwI 672 GATCC GGATC(4/5) ApaLI 697 GTGCAC G^TGCAC BbrPI 651 CACGTG CAC^GTG BsaAI 651 CACGTG [CT]AC^GT[GA] BsaI 7 GAGACC GGTCTC(1/5) BsgI 538 CTGCAC GTGCAG(16/14) BsiWI 603 CGTACG C^GTACG BsmBI 250 GAGACG CGTCTC(1/5) BssSI 442 CACGAG CACGAG(-5/-1) BstAPI 387 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 671 AGATCC [GA]^GATC[CT] DraII 142 AGGTCCC [GA]G^G[ACGT]CC[CT] DraIII 552 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 512 TGGCCG [CT]^GGCC[GA] EarI 82 CTCTTC CTCTTC(1/4) EcoO109I 142 AGGTCCC [GA]G^G[ACGT]CC[CT] HincII 547 GTCGAC GT[CT]^[GA]AC HindII 547 GTCGAC GT[CT]^[GA]AC Ksp632I 82 CTCTTC CTCTTC(1/4) KspI 587 CCGCGG CCGC^GG MboII 83 TCTTC GAAGA(8/7) MflI 671 AGATCC [GA]^GATC[CT] MslI 123 CACCACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 310 GCCGGC GCC^GGC NgoMIV 310 GCCGGC G^CCGGC NlaIII 87 CATG CATG^ PmaCI 651 CACGTG CAC^GTG PmlI 651 CACGTG CAC^GTG PpuMI 142 AGGTCCC [GA]G^G[AT]CC[CT] SacII 587 CCGCGG CCGC^GG SalI 547 GTCGAC G^TCGAC SapI 81 GCTCTTC GCTCTTC(1/4) SfaNI 627 GATGC GCATC(5/9) SmlI 100 CTCAAG C^T[CT][GA]AG Tsp45I 59 GTCAC ^GT[GC]AC TspRI 61 CACTG CA[GC]TG[ACGT][ACGT]^ XhoII 671 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AflIII 650 ACACGT A^C[GA][CT]GT 652 ACGTGT AlwNI 385 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 388 CAGCTGCTG Asp700I 159 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 636 GAAAGAGTTC AspHI 439 GAGCAC G[AT]GC[AT]^C 697 GTGCAC AvaII 90 GGTCC G^G[AT]CC 143 GGTCC BbeI 282 GGCGCC GGCGC^C 576 GGCGCC BseRI 97 CTCCTC GAGGAG(10/8) 247 GAGGAG BsiHKAI 439 GAGCAC G[AT]GC[AT]^C 697 GTGCAC BsmAI 7 GAGAC GTCTC(1/5) 250 GAGAC BsmFI 144 GTCCC GGGAC(10/14) 474 GTCCC BspMI 422 ACCTGC ACCTGC(4/8) 140 GCAGGT BsrFI 310 GCCGGC [GA]^CCGG[CT] 686 ACCGGC BstF5I 189 CATCC GGATG(2/0) 582 CATCC Cfr10I 310 GCCGGC [GA]^CCGG[CT] 686 ACCGGC DpnII 231 GATC ^GATC 672 GATC DsaI 125 CCACGG C^C[GA][CT]GG 587 CCGCGG FokI 189 CATCC GGATG(9/13) 582 CATCC HgaI 208 GACGC GACGC(5/10) 252 GACGC HinfI 298 GAGTC G^A[ACGT]TC 472 GAGTC HphI 116 TCACC GGTGA(8/7) 122 TCACC KasI 282 GGCGCC G^GCGCC 576 GGCGCC MaeIII 59 GTCAC ^GT[ACGT]AC 290 GTTAC MboI 231 GATC ^GATC 672 GATC NarI 282 GGCGCC GG^CGCC 576 GGCGCC NdeII 231 GATC ^GATC 672 GATC PleI 298 GAGTC GAGTC(4/5) 472 GAGTC PstI 226 CTGCAG CTGCA^G 519 CTGCAG Sau3AI 231 GATC ^GATC 672 GATC SfcI 226 CTGCAG C^T[GA][CT]AG 519 CTGCAG SfoI 282 GGCGCC GGC^GCC 576 GGCGCC TaqI 233 TCGA T^CGA 548 TCGA TthHB8I 233 TCGA T^CGA 548 TCGA XmnI 159 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 636 GAAAGAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AccII AciI AcyI AfaI AluI BanI BbvI BcnI BmyI BpmI BsaHI BsaJI BsiYI BslI Bsp1286I BssHII BssKI BstNI BstUI Cac8I CfoI Cfr13I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI MaeII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV PvuII RsaI Sau96I ScrFI TseI