(Linear) (Six-base) MAPSORT of: KIEE5244.seq Check: 9927 from: 1 to: 708 KIEE5244.orf With 182 enzymes: SgfI * January 29, 2014 11:52 .. AccI GT'mk_AC Cuts at: 0 548 708 Size: 548 160 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 178 708 Size: 178 530 AflIII A'CryG_T Cuts at: 0 650 652 708 Size: 650 2 56 Fragments arranged by size: 650 56 2 AleI CACnn'nnGTG Cuts at: 0 127 708 Size: 127 581 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 544 708 Size: 544 164 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 625 657 708 Size: 625 32 51 Fragments arranged by size: 625 51 32 AlwNI CAG_nnn'CTG Cuts at: 0 390 393 708 Size: 390 3 315 Fragments arranged by size: 390 315 3 ApaLI G'TGCA_C Cuts at: 0 697 708 Size: 697 11 BanI G'GyrC_C Cuts at: 0 282 357 576 708 Size: 282 75 219 132 Fragments arranged by size: 282 219 132 75 BbeI G_GCGC'C Cuts at: 0 286 580 708 Size: 286 294 128 Fragments arranged by size: 294 286 128 Bme1580I G_kGCm'C Cuts at: 0 362 701 708 Size: 362 339 7 Fragments arranged by size: 362 339 7 BmgBI CAC'GTC Cuts at: 0 153 708 Size: 153 555 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1 265 457 708 Size: 1 264 192 251 Fragments arranged by size: 264 251 192 1 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 85 708 Size: 85 623 BsaI GGTCTCn'nnnn_ Cuts at: 0 1 708 Size: 1 707 BsaAI yAC'GTr Cuts at: 0 653 708 Size: 653 55 BsaHI Gr'CG_yC Cuts at: 0 209 283 577 708 Size: 209 74 294 131 Fragments arranged by size: 294 209 131 74 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 52 82 319 349 625 655 708 Size: 52 30 237 30 276 30 53 Fragments arranged by size: 276 237 53 52 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 88 262 708 Size: 88 174 446 Fragments arranged by size: 446 174 88 BseYI C'CCAG_C Cuts at: 0 255 274 708 Size: 255 19 434 Fragments arranged by size: 434 255 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 523 708 Size: 523 185 BsiHKAI G_wGCw'C Cuts at: 0 443 701 708 Size: 443 258 7 Fragments arranged by size: 443 258 7 BsiWI C'GTAC_G Cuts at: 0 603 708 Size: 603 105 BsmBI CGTCTCn'nnnn_ Cuts at: 0 244 708 Size: 244 464 Bsp1286I G_dGCh'C Cuts at: 0 362 443 701 708 Size: 362 81 258 7 Fragments arranged by size: 362 258 81 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 131 431 708 Size: 131 300 277 Fragments arranged by size: 300 277 131 BsrFI r'CCGG_y Cuts at: 0 310 686 708 Size: 310 376 22 Fragments arranged by size: 376 310 22 BssHII G'CGCG_C Cuts at: 0 396 426 428 560 708 Size: 396 30 2 132 148 Fragments arranged by size: 396 148 132 30 2 BssSI C'ACGA_G Cuts at: 0 442 708 Size: 442 266 BstAPI GCAn_nnn'nTGC Cuts at: 0 393 708 Size: 393 315 BstYI r'GATC_y Cuts at: 0 671 708 Size: 671 37 BtgI C'CryG_G Cuts at: 0 125 587 708 Size: 125 462 121 Fragments arranged by size: 462 125 121 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 640 708 Size: 640 68 BtsI GCAGTG_nn' Cuts at: 0 60 708 Size: 60 648 DraIII CAC_nnn'GTG Cuts at: 0 557 708 Size: 557 151 EaeI y'GGCC_r Cuts at: 0 512 708 Size: 512 196 EarI CTCTTCn'nnn_ Cuts at: 0 88 708 Size: 88 620 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 559 708 Size: 559 149 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1 178 265 457 708 Size: 1 177 87 192 251 Fragments arranged by size: 251 192 177 87 1 EcoO109I rG'GnC_Cy Cuts at: 0 143 708 Size: 143 565 HaeII r_GCGC'y Cuts at: 0 286 580 668 708 Size: 286 294 88 40 Fragments arranged by size: 294 286 88 40 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 42 74 708 Size: 42 32 634 Fragments arranged by size: 634 42 32 HincII GTy'rAC Cuts at: 0 549 708 Size: 549 159 KasI G'GCGC_C Cuts at: 0 282 576 708 Size: 282 294 132 Fragments arranged by size: 294 282 132 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 326 708 Size: 326 382 MslI CAynn'nnrTG Cuts at: 0 127 708 Size: 127 581 MspA1I CmG'CkG Cuts at: 0 21 78 183 390 465 589 708 Size: 21 57 105 207 75 124 119 Fragments arranged by size: 207 124 119 105 75 57 21 NaeI GCC'GGC Cuts at: 0 312 708 Size: 312 396 NarI GG'CG_CC Cuts at: 0 283 577 708 Size: 283 294 131 Fragments arranged by size: 294 283 131 NgoMIV G'CCGG_C Cuts at: 0 310 708 Size: 310 398 PfoI T'CCnGG_A Cuts at: 0 488 708 Size: 488 220 PmlI CAC'GTG Cuts at: 0 653 708 Size: 653 55 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 625 657 708 Size: 625 32 51 Fragments arranged by size: 625 51 32 PpuMI rG'GwC_Cy Cuts at: 0 143 708 Size: 143 565 PstI C_TGCA'G Cuts at: 0 230 523 708 Size: 230 293 185 Fragments arranged by size: 293 230 185 PvuII CAG'CTG Cuts at: 0 21 183 390 465 708 Size: 21 162 207 75 243 Fragments arranged by size: 243 207 162 75 21 SacII CC_GC'GG Cuts at: 0 590 708 Size: 590 118 SalI G'TCGA_C Cuts at: 0 547 708 Size: 547 161 SapI GCTCTTCn'nnn_ Cuts at: 0 88 708 Size: 88 620 SfcI C'TryA_G Cuts at: 0 226 519 708 Size: 226 293 189 Fragments arranged by size: 293 226 189 SfoI GGC'GCC Cuts at: 0 284 578 708 Size: 284 294 130 Fragments arranged by size: 294 284 130 SmlI C'TyrA_G Cuts at: 0 100 708 Size: 100 608 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 52 84 319 351 708 Size: 52 32 235 32 357 Fragments arranged by size: 357 235 52 32 32 XmnI GAAnn'nnTTC Cuts at: 0 163 640 708 Size: 163 477 68 Fragments arranged by size: 477 163 68 Enzymes that do cut: AccI AcuI AflIII AleI AlfI AloI AlwNI ApaLI BanI BbeI Bme1580I BmgBI BpmI BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI BsmBI Bsp1286I BspMI BsrFI BssHII BssSI BstAPI BstYI BtgI BtgZI BtsI DraIII EaeI EarI EciI Eco57MI EcoO109I HaeII Hin4I HincII KasI MmeI MslI MspA1I NaeI NarI NgoMIV PfoI PmlI PpiI PpuMI PstI PvuII SacII SalI SapI SfcI SfoI SmlI TstI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI ApaI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmrI BmtI BplI Bpu10I BsaBI BsaWI BsiEI BsmI BspEI BspHI BsrBI BsrDI BsrGI BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MscI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmaI ZraI