################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI ApaLI ApoI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BanI BbeI BbsI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaWI BseAI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI Bst1107I BstBI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII Eam1105I Eco81I EcoRI EcoRV EcoT22I FbaI FspI HincII HindII HindIII HpaI KasI KpnI MamI MfeI MluI MroI MseI MunI NarI NcoI NdeI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SalI SbfI ScaI SexAI SfcI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccI 1189 GTAGAC GT^[AC][GT]AC AcyI 667 GACGCC G[GA]^CG[CT]C AlwI 96 GATCC GGATC(4/5) AlwNI 189 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG AscI 561 GGCGCGCC GG^CGCGCC BalI 389 TGGCCA TGG^CCA BbrPI 568 CACGTG CAC^GTG BbvCI 772 GCTGAGG CCTCAGC(-5/-2) BcgI 1152 CGACTTCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 58 CTAG C^TAG BsaAI 568 CACGTG [CT]AC^GT[GA] BsaHI 667 GACGCC G[GA]^CG[CT]C BsaI 777 GGTCTC GGTCTC(1/5) BseRI 1012 CTCCTC GAGGAG(10/8) BssSI 430 CTCGTG CACGAG(-5/-1) BstEII 1144 GGTTACC G^GT[ACGT]ACC BstPI 1144 GGTTACC G^GT[ACGT]ACC BstYI 95 AGATCC [GA]^GATC[CT] DpnII 96 GATC ^GATC DraII 1075 GGGCCCC [GA]G^G[ACGT]CC[CT] DrdI 769 GACGCTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 411 CCTCATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1075 GGGCCCC [GA]G^G[ACGT]CC[CT] EcoO65I 1144 GGTTACC G^GT[ACGT]ACC FseI 865 GGCCGGCC GGCCGG^CC Hin1I 667 GACGCC G[GA]^CG[CT]C HinfI 34 GAGTC G^A[ACGT]TC HphI 506 TCACC GGTGA(8/7) KspI 717 CCGCGG CCGC^GG MaeI 58 CTAG C^TAG MaeII 569 ACGT A^CGT MboI 96 GATC ^GATC MflI 95 AGATCC [GA]^GATC[CT] MluNI 389 TGGCCA TGG^CCA MscI 389 TGGCCA TGG^CCA NdeII 96 GATC ^GATC NheI 57 GCTAGC G^CTAGC NotI 489 GCGGCCGC GC^GGCCGC NspI 605 ACATGC [GA]CATG^[CT] PflMI 855 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 34 GAGTC GAGTC(4/5) PmaCI 568 CACGTG CAC^GTG PmlI 568 CACGTG CAC^GTG SacII 717 CCGCGG CCGC^GG SapI 531 GAAGAGC GCTCTTC(1/4) Sau3AI 96 GATC ^GATC SfaNI 302 GCATC GCATC(5/9) SfiI 910 GGCCTGGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 29 CCCGGG CCC^GGG Tsp45I 116 GTGAC ^GT[GC]AC Van91I 855 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 95 AGATCC [GA]^GATC[CT] XmaCI 29 CCCGGG C^CCGGG XmaI 29 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### BsiEI 490 CGGCCG CG[GA][CT]^CG 672 CGGCCG BsmBI 523 GAGACG CGTCTC(1/5) 592 GAGACG BssHII 7 GCGCGC G^CGCGC 562 GCGCGC BstAPI 188 GCAGCGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 315 GCAGGTGGTGC BstXI 311 CCACGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 512 CCAAGCAGCTGG DdeI 773 CTGAG C^T[ACGT]AG 1199 CTCAG EagI 490 CGGCCG C^GGCCG 672 CGGCCG EarI 531 GAAGAG CTCTTC(1/4) 651 GAAGAG EclXI 490 CGGCCG C^GGCCG 672 CGGCCG Eco52I 490 CGGCCG C^GGCCG 672 CGGCCG Ksp632I 531 GAAGAG CTCTTC(1/4) 651 GAAGAG MaeIII 116 GTGAC ^GT[ACGT]AC 1145 GTTAC SmlI 145 CTCAAG C^T[CT][GA]AG 181 CTCAAG StuI 857 AGGCCT AGG^CCT 909 AGGCCT XcmI 851 CCACCCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1085 CCATGAGGGTGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AccII AciI AfaI AluI Aor51HI ApaI AvaI AvaII BanII BbvI BcnI BglI BmyI BpmI BsaJI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DsaI EaeI Eco47III EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII ItaI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NgoMIV NlaIII NlaIV PvuII RsaI Sau96I ScrFI StyI TseI TspRI