(Circular) (Six-base) MAPSORT of: pFN21AB9719.seq Check: 2297 from: 1 to: 5911 pFN21AB9719 5911 bp With 182 enzymes: SgfI * August 16, 2010 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2311 2364 2311 Size: 53 5858 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3721 278 Size: 53 83 186 936 2185 2468 Fragments arranged by size: 2468 2185 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3195 3254 1937 Size: 1258 59 4594 Fragments arranged by size: 4594 1258 59 Acc65I G'GTAC_C Cuts at: 3232 3232 Size: 5911 AclI AA'CG_TT Cuts at: 1563 4038 4411 1563 Size: 2475 373 3063 Fragments arranged by size: 3063 2475 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2212 2555 2684 3974 5358 5626 1632 Size: 580 343 129 1290 1384 268 1917 Fragments arranged by size: 1917 1384 1290 580 343 268 129 AfeI AGC'GCT Cuts at: 2222 2273 2542 2687 2717 2222 Size: 51 269 145 30 5416 Fragments arranged by size: 5416 269 145 51 30 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5714 AflIII A'CryG_T Cuts at: 1203 4810 5787 1203 Size: 3607 977 1327 Fragments arranged by size: 3607 1327 977 AgeI A'CCGG_T Cuts at: 4742 4742 Size: 5911 AhdI GACnn_n'nnGTC Cuts at: 4640 4640 Size: 5911 AleI CACnn'nnGTG Cuts at: 1166 2321 1166 Size: 1155 4756 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2382 1184 Size: 1198 4713 AlwNI CAG_nnn'CTG Cuts at: 2199 3654 5226 2199 Size: 1455 1572 2884 Fragments arranged by size: 2884 1572 1455 ApaI G_GGCC'C Cuts at: 2030 2822 3084 2030 Size: 792 262 4857 Fragments arranged by size: 4857 792 262 ApaLI G'TGCA_C Cuts at: 3968 5124 3968 Size: 1156 4755 ApoI r'AATT_y Cuts at: 1493 3220 3446 3598 5707 1493 Size: 1727 226 152 2109 1697 Fragments arranged by size: 2109 1727 1697 226 152 AscI GG'CGCG_CC Cuts at: 2567 2567 Size: 5911 AseI AT'TA_AT Cuts at: 160 1032 4465 160 Size: 872 3433 1606 Fragments arranged by size: 3433 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5911 AvaI C'yCGr_G Cuts at: 1870 1943 2034 2878 3064 3236 1870 Size: 73 91 844 186 172 4545 Fragments arranged by size: 4545 844 186 172 91 73 BamHI G'GATC_C Cuts at: 3241 3608 3241 Size: 367 5544 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3232 4687 618 Size: 325 217 627 1445 1455 1842 Fragments arranged by size: 1842 1455 1445 627 325 217 BanII G_rGCy'C Cuts at: 1391 2030 2822 2887 3073 3084 3230 1391 Size: 639 792 65 186 11 146 4072 Fragments arranged by size: 4072 792 639 186 146 65 11 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4940 BbvCI CC'TCA_GC Cuts at: 2778 2778 Size: 5911 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3146 3180 4102 4136 1903 Size: 34 1209 34 922 34 3678 Fragments arranged by size: 3678 1209 922 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3803 5013 3803 Size: 1210 4701 BclI T'GATC_A Cuts at: 1548 1548 Size: 5911 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2304 2922 Size: 107 122 71 631 605 337 295 618 Cuts at: 2922 3003 4522 136 Size: 81 1519 1525 Fragments arranged by size: 1525 1519 631 618 605 337 295 122 107 81 71 BglII A'GATC_T Cuts at: 5906 5906 Size: 5911 BlpI GC'TnA_GC Cuts at: 3328 3328 Size: 5911 Bme1580I G_kGCm'C Cuts at: 1790 2030 2822 3063 3084 3972 5128 1790 Size: 240 792 241 21 888 1156 2573 Fragments arranged by size: 2573 1156 888 792 241 240 21 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5911 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3111 3642 4600 Size: 416 53 83 399 24 1694 531 958 Cuts at: 4600 442 Size: 1753 Fragments arranged by size: 1753 1694 958 531 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2066 1056 Size: 1010 4901 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2605 2605 Size: 5911 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2546 2924 3007 4571 1239 Size: 1307 378 83 1564 2579 Fragments arranged by size: 2579 1564 1307 378 83 Bpu10I CC'TnA_GC Cuts at: 2778 2778 Size: 5911 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2135 2171 4036 4901 5199 2135 Size: 36 1865 865 298 2847 Fragments arranged by size: 2847 1865 865 298 36 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2788 4574 882 Size: 617 1289 1786 2219 Fragments arranged by size: 2219 1786 1289 617 BsaAI yAC'GTr Cuts at: 493 1204 2575 3702 493 Size: 711 1371 1127 2702 Fragments arranged by size: 2702 1371 1127 711 BsaBI GATnn'nnATC Cuts at: 5905 5905 Size: 5911 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2673 3718 4100 4735 Size: 53 83 186 936 1140 1045 382 635 Cuts at: 4735 275 Size: 1451 Fragments arranged by size: 1451 1140 1045 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4344 4742 5016 5163 5574 4344 Size: 398 274 147 411 4681 Fragments arranged by size: 4681 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5686 Fragments arranged by size: 5686 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3008 1186 Size: 1822 4089 BseYI C'CCAG_C Cuts at: 2023 2302 2688 3009 5114 5610 2023 Size: 279 386 321 2105 496 2324 Fragments arranged by size: 2324 2105 496 386 321 279 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2212 2342 2642 1163 Size: 573 476 130 300 4432 Fragments arranged by size: 4432 573 476 300 130 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2498 2680 3361 4122 5150 1540 Size: 275 186 497 182 681 761 1028 2301 Fragments arranged by size: 2301 1028 761 681 497 275 186 182 BsiHKAI G_wGCw'C Cuts at: 3972 4057 5128 3972 Size: 85 1071 4755 Fragments arranged by size: 4755 1071 85 BsmI GAATG_Cn' Cuts at: 3422 3515 3422 Size: 93 5818 BsmBI CGTCTCn'nnnn_ Cuts at: 2522 2591 2522 Size: 69 5842 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2030 2822 2887 3063 3073 3084 3230 Size: 399 240 792 65 176 10 11 146 Cuts at: 3230 3972 4057 5128 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 792 742 399 240 176 146 85 65 11 10 BspEI T'CCGG_A Cuts at: 5574 5574 Size: 5911 BspHI T'CATG_A Cuts at: 3800 5530 3800 Size: 1730 4181 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2064 2097 2311 2364 2614 3266 844 Size: 1220 33 214 53 250 652 3489 Fragments arranged by size: 3489 1220 652 250 214 53 33 BsrBI CCG'CTC Cuts at: 1394 1823 3798 1394 Size: 429 1975 3507 Fragments arranged by size: 3507 1975 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4406 4580 65 Size: 1183 3158 174 1396 Fragments arranged by size: 3158 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2430 2563 2675 2679 2871 4555 4742 Size: 110 620 133 112 4 192 1684 187 Cuts at: 4742 1700 Size: 2869 Fragments arranged by size: 2869 1684 620 192 187 133 112 110 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5911 BssHII G'CGCG_C Cuts at: 1925 2012 2567 1925 Size: 87 555 5269 Fragments arranged by size: 5269 555 87 BssSI C'ACGA_G Cuts at: 2435 3971 4983 2435 Size: 1536 1012 3363 Fragments arranged by size: 3363 1536 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2199 2326 794 Size: 1405 127 4379 Fragments arranged by size: 4379 1405 127 BstBI TT'CG_AA Cuts at: 4715 4715 Size: 5911 BstEII G'GTnAC_C Cuts at: 1184 3149 1184 Size: 1965 3946 BstXI CCAn_nnnn'nTGG Cuts at: 2323 2524 2323 Size: 201 5710 BstYI r'GATC_y Cuts at: 1972 2100 3241 3608 3993 4010 5462 5548 5560 Size: 128 1141 367 385 17 1452 86 12 Cuts at: 5560 5906 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1141 385 367 346 128 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2397 2484 2722 3132 513 Size: 552 939 393 87 238 410 3292 Fragments arranged by size: 3292 939 552 410 393 238 87 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5172 Fragments arranged by size: 5172 683 56 BtsI GCAGTG_nn' Cuts at: 2066 2359 3427 4221 4241 2066 Size: 293 1068 794 20 3736 Fragments arranged by size: 3736 1068 794 293 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5911 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5876 DraI TTT'AAA Cuts at: 3215 3215 Size: 5911 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2780 4918 809 Size: 507 1464 2138 1802 Fragments arranged by size: 2138 1802 1464 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2214 2394 2487 Size: 54 1425 50 275 18 384 180 93 Cuts at: 2487 2495 2677 2823 3358 4247 5876 8 Size: 8 182 146 535 889 1629 43 Fragments arranged by size: 1629 1425 889 535 384 275 182 180 146 93 54 50 43 18 8 EagI C'GGCC_G Cuts at: 1537 1812 2495 2677 3358 1537 Size: 275 683 182 681 4090 Fragments arranged by size: 4090 683 681 275 182 EarI CTCTTCn'nnn_ Cuts at: 1353 2531 2651 3841 1353 Size: 1178 120 1190 3423 Fragments arranged by size: 3423 1190 1178 120 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2099 2462 2825 4498 4872 5018 Size: 1064 138 510 363 363 1673 374 146 Cuts at: 5018 387 Size: 1280 Fragments arranged by size: 1673 1280 1064 510 374 363 363 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2212 2546 2555 2684 2924 3007 3974 Size: 393 580 334 9 129 240 83 967 Cuts at: 3974 4571 5358 5626 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 967 787 597 580 393 334 268 240 129 83 9 EcoNI CCTnn'n_nnAGG Cuts at: 2420 2420 Size: 5911 EcoO109I rG'GnC_Cy Cuts at: 3081 3081 Size: 5911 EcoRI G'AATT_C Cuts at: 3220 3598 3220 Size: 378 5533 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2278 2278 Size: 5911 FseI GG_CCGG'CC Cuts at: 2875 2875 Size: 5911 FspI TGC'GCA Cuts at: 4417 4417 Size: 5911 HaeII r_GCGC'y Cuts at: 1678 2224 2275 2544 2689 2719 5058 1678 Size: 546 51 269 145 30 2339 2531 Fragments arranged by size: 2531 2339 546 269 145 51 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4552 4584 4626 4658 1373 Size: 32 135 32 2980 32 42 32 2626 Fragments arranged by size: 2980 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3255 3501 669 Size: 988 281 1317 246 3079 Fragments arranged by size: 3079 1317 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5911 HpaI GTT'AAC Cuts at: 3501 3501 Size: 5911 KpnI G_GTAC'C Cuts at: 3236 3236 Size: 5911 MfeI C'AATT_G Cuts at: 3510 3510 Size: 5911 MluI A'CGCG_T Cuts at: 5787 5787 Size: 5911 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2718 5025 5209 1305 Size: 1413 2307 184 2007 Fragments arranged by size: 2307 2007 1413 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2396 10 Size: 54 1425 343 564 3525 Fragments arranged by size: 3525 1425 564 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2321 2615 3095 3869 4228 4387 518 Size: 648 1155 294 480 774 359 159 2042 Fragments arranged by size: 2042 1155 774 648 480 359 294 159 MspA1I CmG'CkG Cuts at: 1606 2107 2302 2392 2524 2724 3144 3327 4004 Size: 501 195 90 132 200 420 183 677 Cuts at: 4004 5152 5397 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 501 420 245 200 195 183 132 90 NaeI GCC'GGC Cuts at: 2432 2565 2677 2681 2873 2432 Size: 133 112 4 192 5470 Fragments arranged by size: 5470 192 133 112 4 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4420 Fragments arranged by size: 4420 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5911 NgoMIV G'CCGG_C Cuts at: 2430 2563 2675 2679 2871 2430 Size: 133 112 4 192 5470 Fragments arranged by size: 5470 192 133 112 4 NheI G'CTAG_C Cuts at: 1052 2062 1052 Size: 1010 4901 NotI GC'GGCC_GC Cuts at: 2495 3358 2495 Size: 863 5048 NspI r_CATG'y Cuts at: 1132 1229 2614 3269 4814 1132 Size: 97 1385 655 1545 2229 Fragments arranged by size: 2229 1545 1385 655 97 PasI CC'CwG_GG Cuts at: 1348 2887 1348 Size: 1539 4372 PciI A'CATG_T Cuts at: 4810 4810 Size: 5911 PflMI CCAn_nnn'nTGG Cuts at: 1108 2866 1108 Size: 1758 4153 PfoI T'CCnGG_A Cuts at: 2133 2133 Size: 5911 PmeI GTTT'AAAC Cuts at: 3215 3215 Size: 5911 PmlI CAC'GTG Cuts at: 2575 2575 Size: 5911 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3980 4012 5493 5525 3980 Size: 32 1481 32 4366 Fragments arranged by size: 4366 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5911 PsiI TTA'TAA Cuts at: 3481 3481 Size: 5911 PspOMI G'GGCC_C Cuts at: 2026 2818 3080 2026 Size: 792 262 4857 Fragments arranged by size: 4857 792 262 PstI C_TGCA'G Cuts at: 830 3263 830 Size: 2433 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5911 PvuII CAG'CTG Cuts at: 2302 2392 2524 2302 Size: 90 132 5689 Fragments arranged by size: 5689 132 90 SacII CC_GC'GG Cuts at: 2725 2725 Size: 5911 SalI G'TCGA_C Cuts at: 1936 3253 1936 Size: 1317 4594 SapI GCTCTTCn'nnn_ Cuts at: 2531 2531 Size: 5911 SbfI CC_TGCA'GG Cuts at: 3263 3263 Size: 5911 ScaI AGT'ACT Cuts at: 4159 5871 4159 Size: 1712 4199 SfcI C'TryA_G Cuts at: 826 1045 3259 4394 5075 5266 826 Size: 219 2214 1135 681 191 1471 Fragments arranged by size: 2214 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2922 2922 Size: 5911 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5911 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5911 SmaI CCC'GGG Cuts at: 1872 2036 3238 1872 Size: 164 1202 4545 Fragments arranged by size: 4545 1202 164 SmlI C'TyrA_G Cuts at: 820 1017 1943 2150 2186 4015 4916 5178 820 Size: 197 926 207 36 1829 901 262 1553 Fragments arranged by size: 1829 1553 926 901 262 207 197 36 SnaBI TAC'GTA Cuts at: 493 3702 493 Size: 3209 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5911 SphI G_CATG'C Cuts at: 1132 3269 1132 Size: 2137 3774 SspI AAT'ATT Cuts at: 5 52 3835 5 Size: 47 3783 2081 Fragments arranged by size: 3783 2081 47 StuI AGG'CCT Cuts at: 1517 2864 2916 1517 Size: 1347 52 4512 Fragments arranged by size: 4512 1347 52 StyI C'CwwG_G Cuts at: 513 1065 2004 2649 2805 2865 3350 513 Size: 552 939 645 156 60 485 3074 Fragments arranged by size: 3074 939 645 552 485 156 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2873 3103 3938 3955 4108 4293 4738 Size: 1255 1293 230 835 17 153 185 445 Cuts at: 4738 325 Size: 1498 Fragments arranged by size: 1498 1293 1255 835 445 230 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4157 5869 96 Size: 274 80 33 51 1443 2180 1712 138 Fragments arranged by size: 2180 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5871 5903 5871 Size: 32 5879 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5911 XbaI T'CTAG_A Cuts at: 3247 3247 Size: 5911 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2863 3097 2863 Size: 234 5677 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5911 XmaI C'CCGG_G Cuts at: 1870 2034 3236 1870 Size: 164 1202 4545 Fragments arranged by size: 4545 1202 164 XmnI GAAnn'nnTTC Cuts at: 4040 4040 Size: 5911 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3719 276 Size: 53 83 186 936 2185 2468 Fragments arranged by size: 2468 2185 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FspAI KasI NarI NruI NsiI PacI PpuMI PspXI PsrI RsrII SacI SanDI SexAI SfoI SrfI SwaI