################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI AspEI AspHI AspI AviII AvrII BalI BanII BbrPI BbvCI BciVI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaWI BseAI BsgI BsiHKAI BsiWI BsmBI BsmFI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI DsaI Eam1105I Eco47III Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI FspI HincII HindII HindIII HpaI KspI MaeI MaeIII MamI MfeI MluI MluNI MroI MscI MseI MslI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp45I Tsp509I Tth111I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 77 GGTACC G^GTACC Asp700I 198 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 77 GGTACC G^GTACC AvaI 22 CCCGGG C^[CT]CG[GA]G AvaII 279 GGACC G^G[AT]CC BamHI 452 GGATCC G^GATCC BbsI 307 GTCTTC GAAGAC(2/6) BcgI 371 GCAACGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 191 TGATCA T^GATCA BpmI 502 CTGGAG CTGGAG(16/14) BpuAI 307 GTCTTC GAAGAC(2/6) BsaI 496 GAGACC GGTCTC(1/5) BseRI 481 GAGGAG GAGGAG(10/8) BsmI 198 GAATGC GAATGC(1/-1) BsoBI 22 CCCGGG C^[CT]CG[GA]G BspHI 194 TCATGA T^CATGA BsrI 240 CCAGT ACTGG(1/-1) BstXI 359 CCACGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 452 GGATCC [GA]^GATC[CT] Cfr13I 279 GGACC G^G[ACGT]CC DdeI 9 CTCAG C^T[ACGT]AG EaeI 176 CGGCCG [CT]^GGCC[GA] EagI 176 CGGCCG C^GGCCG EclXI 176 CGGCCG C^GGCCG Eco52I 176 CGGCCG C^GGCCG EcoNI 443 CCTATGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 191 TGATCA T^GATCA HgaI 28 GCGTC GACGC(5/10) HinfI 407 GACTC G^A[ACGT]TC HphI 152 TCACC GGTGA(8/7) KpnI 77 GGTACC GGTAC^C MaeII 70 ACGT A^CGT MflI 452 GGATCC [GA]^GATC[CT] MspA1I 81 CCGCTG C[AC]G^C[GT]G NaeI 178 GCCGGC GCC^GGC NgoMIV 178 GCCGGC G^CCGGC PleI 407 GACTC GAGTC(4/5) RcaI 194 TCATGA T^CATGA Sau96I 279 GGACC G^G[ACGT]CC SmaI 22 CCCGGG CCC^GGG TaqI 248 TCGA T^CGA TspRI 263 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 248 TCGA T^CGA XhoII 452 GGATCC [GA]^GATC[CT] XmaCI 22 CCCGGG C^CCGGG XmaI 22 CCCGGG C^CCGGG XmnI 198 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AfaI 78 GTAC GT^AC 402 GTAC AlwI 453 GATCC GGATC(4/5) 452 GGATC BbeI 18 GGCGCC GGCGC^C 225 GGCGCC BcnI 22 CCCGG CC^[GC]GG 23 CCGGG BsiEI 176 CGGCCG CG[GA][CT]^CG 326 CGGTCG BsmAI 496 GAGAC GTCTC(1/5) 118 GTCTC BsrFI 178 GCCGGC [GA]^CCGG[CT] 281 ACCGGC Cfr10I 178 GCCGGC [GA]^CCGG[CT] 281 ACCGGC EarI 139 CTCTTC CTCTTC(1/4) 58 GAAGAG FauI 49 CCCGC CCCGC(4/6) 317 GCGGG HaeII 18 GGCGCC [GA]GCGC^[CT] 225 GGCGCC HaeIII 136 GGCC GG^CC 177 GGCC KasI 18 GGCGCC G^GCGCC 225 GGCGCC Ksp632I 139 CTCTTC CTCTTC(1/4) 58 GAAGAG NarI 18 GGCGCC GG^CGCC 225 GGCGCC NciI 22 CCCGG CC^[GC]GG 23 CCGGG NlaIII 66 CATG CATG^ 195 CATG RsaI 78 GTAC GT^AC 402 GTAC SfaNI 433 GATGC GCATC(5/9) 149 GCATC SfoI 18 GGCGCC GGC^GCC 225 GGCGCC ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AccII AciI AcyI AluI BanI BbvI BsaHI BsaJI BsiYI BslI BssKI BstF5I BstNI BstUI Cac8I CfoI DpnII EcoRII Fnu4HI FokI HapII HhaI Hin1I HinP1I HpaII ItaI MboI MboII MnlI MspI MvaI MvnI MwoI NdeII NlaIV Sau3AI ScrFI TseI