################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## AatII AccIII AclI AcsI AflII AgeI ApoI BbvCI BciVI BclI BfrI BglII BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BsrGI Bst1107I BstBI BstZ17I ClaI CpoI DraI EagI EclXI Eco52I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI Psp1406I PvuI RsrII SacI SalI SbfI SfiI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tsp509I XbaI XhoI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### Acc65I 4233 GGTACC G^GTACC AccI 1747 GTCTAC GT^[AC][GT]AC AhdI 4249 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 376 GTGCAC G^TGCAC ApaLI 376 GTGCAC G^TGCAC AscI 706 GGCGCGCC GG^CGCGCC AseI 3985 ATTAAT AT^TAAT AsnI 3985 ATTAAT AT^TAAT Asp700I 337 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 4233 GGTACC G^GTACC AspEI 4249 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 517 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 894 TGCGCA TGC^GCA AvrII 3040 CCTAGG C^CTAGG BcgI 1177 CGAAGTCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 3040 CCTAGG C^CTAGG BsaI 2485 GAGACC GGTCTC(1/5) BsaWI 4236 ACCGGA [AT]^CCGG[AT] BsmBI 2715 CGTCTC CGTCTC(1/5) BspHI 1484 TCATGA T^CATGA BssSI 3954 CACGAG CACGAG(-5/-1) DrdI 649 GACCTGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 4249 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FspI 894 TGCGCA TGC^GCA KpnI 4233 GGTACC GGTAC^C PflFI 517 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 1740 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3985 ATTAAT AT^TAAT RcaI 1484 TCATGA T^CATGA ScaI 4211 AGTACT AGT^ACT SgrAI 3280 CGCCGGCG C[GA]^CCGG[CT]G TfiI 50 GAATC G^A[AT]TC Tth111I 517 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 337 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### Aor51HI 302 AGCGCT AGC^GCT 3818 AGCGCT BamHI 501 GGATCC G^GATCC 2918 GGATCC BspLU11I 1454 ACATGT A^CATGT 3719 ACATGT BssHII 707 GCGCGC G^CGCGC 835 GCGCGC DraIII 379 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG 2976 CACCCAGTG Eco47III 302 AGCGCT AGC^GCT 3818 AGCGCT KspI 856 CCGCGG CCGC^GG 875 CCGCGG MseI 166 TTAA T^TAA 3986 TTAA SacII 856 CCGCGG CCGC^GG 875 CCGCGG SexAI 380 ACCTGGT A^CC[AT]GGT 518 ACCTGGT Tru9I 166 TTAA T^TAA 3986 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 143 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BalI BanI BanII BbeI BbrPI BbsI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI StuI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI