Restriction map for
pFN21AE6742
(Circular) (Six-base) MAPSORT of: pFN21AE6742.seq Check: 3117 from: 1 to: 8986
pFN21AE6742 8986 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2566 5306 6118 2566
Size: 2740 812 5434
Fragments arranged by size:
5434 2740 812
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6796 278
Size: 53 83 186 936 5260 2468
Fragments arranged by size:
5260 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3753 6329 1937
Size: 1816 2576 4594
Fragments arranged by size:
4594 2576 1816
Acc65I G'GTAC_C
Cuts at: 6238 6307 6238
Size: 69 8917
AclI AA'CG_TT
Cuts at: 1563 7113 7486 1563
Size: 5550 373 3063
Fragments arranged by size:
5550 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2102 4355 6209 7049 8433 8701 1632
Size: 470 2253 1854 840 1384 268 1917
Fragments arranged by size:
2253 1917 1854 1384 840 470 268
AfeI AGC'GCT
Cuts at: 2309 5825 2309
Size: 3516 5470
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8789
AflIII A'CryG_T
Cuts at: 1203 2743 3459 4687 4689 5724 7885 8862 1203
Size: 1540 716 1228 2 1035 2161 977 1327
Fragments arranged by size:
2161 1540 1327 1228 1035 977 716 2
AgeI A'CCGG_T
Cuts at: 7817 7817
Size: 8986
AhdI GACnn_n'nnGTC
Cuts at: 6259 7715 6259
Size: 1456 7530
AleI CACnn'nnGTG
Cuts at: 1166 3414 4198 4690 5047 5810 1166
Size: 2248 784 492 357 763 4342
Fragments arranged by size:
4342 2248 784 763 492 357
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4729 5589 5992 1184
Size: 3545 860 403 4178
Fragments arranged by size:
4178 3545 860 403
AlwNI CAG_nnn'CTG
Cuts at: 2414 2727 2815 3376 4532 4996 5577 6729 8301
Size: 313 88 561 1156 464 581 1152 1572
Cuts at: 8301 2414
Size: 3099
Fragments arranged by size:
3099 1572 1156 1152 581 561 464 313
88
ApaI G_GGCC'C
Cuts at: 2273 4086 4372 4945 5391 5550 5922 2273
Size: 1813 286 573 446 159 372 5337
Fragments arranged by size:
5337 1813 573 446 372 286 159
ApaLI G'TGCA_C
Cuts at: 2381 7043 8199 2381
Size: 4662 1156 3168
Fragments arranged by size:
4662 3168 1156
ApoI r'AATT_y
Cuts at: 1493 6295 6521 6673 8782 1493
Size: 4802 226 152 2109 1697
Fragments arranged by size:
4802 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2712 2712
Size: 8986
AseI AT'TA_AT
Cuts at: 160 1032 5991 7540 160
Size: 872 4959 1549 1606
Fragments arranged by size:
4959 1606 1549 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8986
AvaI C'yCGr_G
Cuts at: 1870 1943 2022 2873 3543 4938 5186 5496 5543
Size: 73 79 851 670 1395 248 310 47
Cuts at: 5543 5666 6311 1870
Size: 123 645 4545
Fragments arranged by size:
4545 1395 851 670 645 310 248 123
79 73 47
AvrII C'CTAG_G
Cuts at: 5045 5045
Size: 8986
BamHI G'GATC_C
Cuts at: 2506 4923 6316 6683 2506
Size: 2417 1393 367 4809
Fragments arranged by size:
4809 2417 1393 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2420 2603 2762 2886 3395
Size: 325 217 627 633 183 159 124 509
Cuts at: 3395 3636 4780 5240 5320 5419 5471 6238 6307
Size: 241 1144 460 80 99 52 767 69
Cuts at: 6307 7762 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1144 767 633 627 509 460
325 241 217 183 159 124 99 80
69 52
BanII G_rGCy'C
Cuts at: 1391 2159 2273 2371 3686 4086 4372 4443 4578
Size: 768 114 98 1315 400 286 71 135
Cuts at: 4578 4678 4945 5037 5391 5550 5922 5942 6305
Size: 100 267 92 354 159 372 20 363
Cuts at: 6305 1391
Size: 4072
Fragments arranged by size:
4072 1315 768 400 372 363 354 286
267 159 135 114 100 98 92 71
20
BbeI G_GCGC'C
Cuts at: 2766 3399 5324 2766
Size: 633 1925 6428
Fragments arranged by size:
6428 1925 633
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2108 2136 2272 3329 4343 928
Size: 971 209 28 136 1057 1014 5571
Fragments arranged by size:
5571 1057 1014 971 209 136 28
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3171 3205 7177 7211 1903
Size: 34 50 34 1150 34 3972 34 3678
Fragments arranged by size:
3972 3678 1150 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6878 8088 6878
Size: 1210 7776
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8986
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5171 5533 6210
Size: 107 122 71 631 605 3499 362 677
Cuts at: 6210 7597 136
Size: 1387 1525
Fragments arranged by size:
3499 1525 1387 677 631 605 362 122
107 71
BglII A'GATC_T
Cuts at: 8981 8981
Size: 8986
BlpI GC'TnA_GC
Cuts at: 2459 2816 2825 6403 2459
Size: 357 9 3578 5042
Fragments arranged by size:
5042 3578 357 9
Bme1580I G_kGCm'C
Cuts at: 1790 2273 2385 2425 2688 2889 4086 4372 4859
Size: 483 112 40 263 201 1197 286 487
Cuts at: 4859 4939 4945 5391 5424 5550 5922 7047 8203
Size: 80 6 446 33 126 372 1125 1156
Cuts at: 8203 1790
Size: 2573
Fragments arranged by size:
2573 1197 1156 1125 487 483 446 372
286 263 201 126 112 80 40 33
6
BmgBI CAC'GTC
Cuts at: 1318 5146 1318
Size: 3828 5158
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3421 4249 4978
Size: 416 53 83 399 24 2004 828 729
Cuts at: 4978 6148 6717 7675 442
Size: 1170 569 958 1753
Fragments arranged by size:
2004 1753 1170 958 828 729 569 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8986
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3914 4462 3914
Size: 548 8438
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2270 2573 2641 2720 3272 3353 3887 3935
Size: 1031 303 68 79 552 81 534 48
Cuts at: 3935 3944 4509 4538 4584 5051 5427 5501 5773
Size: 9 565 29 46 467 376 74 272
Cuts at: 5773 7646 1239
Size: 1873 2579
Fragments arranged by size:
2579 1873 1031 565 552 534 467 376
303 272 81 79 74 68 48 46
29 9
Bpu10I CC'TnA_GC
Cuts at: 3560 4044 4572 6195 3560
Size: 484 528 1623 6351
Fragments arranged by size:
6351 1623 528 484
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2173 4662 5615 5714 6121 7111 7976 8274 2173
Size: 2489 953 99 407 990 865 298 2885
Fragments arranged by size:
2885 2489 990 953 865 407 298 99
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4484 7649 882
Size: 617 2985 3165 2219
Fragments arranged by size:
3165 2985 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2746 3666 4690 6777 493
Size: 711 1542 920 1024 2087 2702
Fragments arranged by size:
2702 2087 1542 1024 920 711
BsaBI GATnn'nnATC
Cuts at: 3833 3935 6017 8980 3833
Size: 102 2082 2963 3839
Fragments arranged by size:
3839 2963 2082 102
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2586 2763 3396 5321
Size: 53 83 186 936 1053 177 633 1925
Cuts at: 5321 6793 7175 7810 275
Size: 1472 382 635 1451
Fragments arranged by size:
1925 1472 1451 1053 936 635 633 382
186 177 83 53
BsaWI w'CCGG_w
Cuts at: 6241 7419 7817 8091 8238 8649 6241
Size: 1178 398 274 147 411 6578
Fragments arranged by size:
6578 1178 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3083 3113 3198 3201 3228
Size: 30 165 30 1680 30 85 3 27
Cuts at: 3228 3231 3326 3356 4958 4988 5832 5862 1178
Size: 3 95 30 1602 30 844 30 4302
Fragments arranged by size:
4302 1680 1602 844 165 95 85 30
30 30 30 30 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3044 3222 3373 3941 4007 4979 1186
Size: 1858 178 151 568 66 972 5193
Fragments arranged by size:
5193 1858 972 568 178 151 66
BseYI C'CCAG_C
Cuts at: 2085 2592 2596 2735 3171 3327 3618 3821 4116
Size: 507 4 139 436 156 291 203 295
Cuts at: 4116 4348 4371 4407 4442 4605 5379 5384 5411
Size: 232 23 36 35 163 774 5 27
Cuts at: 5411 5523 6047 8189 8685 2085
Size: 112 524 2142 496 2386
Fragments arranged by size:
2386 2142 774 524 507 496 436 295
291 232 203 163 156 139 112 36
35 27 23 5 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2953 3472 3688 4378 4668 5144 5203
Size: 573 1217 519 216 690 290 476 59
Cuts at: 5203 5585 5664 5785 1163
Size: 382 79 121 4364
Fragments arranged by size:
4364 1217 690 573 519 476 382 290
216 121 79 59
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6436 7197 8225 1540
Size: 275 186 4435 761 1028 2301
Fragments arranged by size:
4435 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2167 2319 2385 2706 2988 3104 4221 4718 5258
Size: 152 66 321 282 116 1117 497 540
Cuts at: 5258 6135 7047 7132 8203 2167
Size: 877 912 85 1071 2950
Fragments arranged by size:
2950 1117 1071 912 877 540 497 321
282 152 116 85 66
BsmI GAATG_Cn'
Cuts at: 6497 6590 6497
Size: 93 8893
BsmBI CGTCTCn'nnnn_
Cuts at: 4726 4726
Size: 8986
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2159 2167 2273 2319 2371 2385 2425
Size: 399 369 8 106 46 52 14 40
Cuts at: 2425 2688 2706 2889 2988 3104 3686 4086 4221
Size: 263 18 183 99 116 582 400 135
Cuts at: 4221 4372 4443 4578 4678 4718 4859 4939 4945
Size: 151 71 135 100 40 141 80 6
Cuts at: 4945 5037 5258 5391 5424 5550 5922 5942 6135
Size: 92 221 133 33 126 372 20 193
Cuts at: 6135 6305 7047 7132 8203 1391
Size: 170 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 582 400 399 372 369
263 221 193 183 170 151 141 135
135 133 126 116 106 100 99 92
85 80 71 52 46 40 40 33
20 18 14 8 6
BspEI T'CCGG_A
Cuts at: 8649 8649
Size: 8986
BspHI T'CATG_A
Cuts at: 3489 6875 8605 3489
Size: 3386 1730 3870
Fragments arranged by size:
3870 3386 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2566 3259 3613 4406 5306 6118 6341 844
Size: 1722 693 354 793 900 812 223 3489
Fragments arranged by size:
3489 1722 900 812 793 693 354 223
BsrBI CCG'CTC
Cuts at: 1394 1823 3120 3198 4297 6873 1394
Size: 429 1297 78 1099 2576 3507
Fragments arranged by size:
3507 2576 1297 1099 429 78
BsrDI GCAATG_nn'
Cuts at: 65 1248 2667 3756 4340 5208 7481 7655 65
Size: 1183 1419 1089 584 868 2273 174 1396
Fragments arranged by size:
2273 1419 1396 1183 1089 868 584 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2571 2777 2805 3909 4827 5286 5359
Size: 110 761 206 28 1104 918 459 73
Cuts at: 5359 5742 5979 7630 7817 1700
Size: 383 237 1651 187 2869
Fragments arranged by size:
2869 1651 1104 918 761 459 383 237
206 187 110 73 28
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8986
BssHII G'CGCG_C
Cuts at: 1925 2712 2840 1925
Size: 787 128 8071
Fragments arranged by size:
8071 787 128
BssSI C'ACGA_G
Cuts at: 5959 7046 8058 5959
Size: 1087 1012 6887
Fragments arranged by size:
6887 1087 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2727 5642 6014 6130 794
Size: 1933 2915 372 116 3650
Fragments arranged by size:
3650 2915 1933 372 116
BstBI TT'CG_AA
Cuts at: 7790 7790
Size: 8986
BstEII G'GTnAC_C
Cuts at: 1184 2567 3217 5677 1184
Size: 1383 650 2460 4493
Fragments arranged by size:
4493 2460 1383 650
BstXI CCAn_nnnn'nTGG
Cuts at: 2681 3647 4200 5049 5809 6040 2681
Size: 966 553 849 760 231 5627
Fragments arranged by size:
5627 966 849 760 553 231
BstYI r'GATC_y
Cuts at: 1972 2506 3930 4923 4968 6316 6683 7068 7085
Size: 534 1424 993 45 1348 367 385 17
Cuts at: 7085 8537 8623 8635 8981 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1424 1348 993 534 385 367
346 86 45 17 12
Bsu36I CC'TnA_GG
Cuts at: 2415 2493 4775 2415
Size: 78 2282 6626
Fragments arranged by size:
6626 2282 78
BtgI C'CryG_G
Cuts at: 513 1065 2265 2861 2880 3674 4049 4652 4945
Size: 552 1200 596 19 794 375 603 293
Cuts at: 4945 6118 513
Size: 1173 3381
Fragments arranged by size:
3381 1200 1173 794 603 596 552 375
293 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8247
Fragments arranged by size:
8247 683 56
BtsI GCAGTG_nn'
Cuts at: 2939 3156 3239 5097 5652 5719 6502 7296 7316
Size: 217 83 1858 555 67 783 794 20
Cuts at: 7316 2939
Size: 4609
Fragments arranged by size:
4609 1858 794 783 555 217 83 67
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8986
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8951
DraI TTT'AAA
Cuts at: 6290 6290
Size: 8986
DraIII CAC_nnn'GTG
Cuts at: 2389 4986 2389
Size: 2597 6389
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2660 7993 809
Size: 507 1344 5333 1802
Fragments arranged by size:
5333 1802 1344 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2049 2262 2512
Size: 54 1425 50 275 18 219 213 250
Cuts at: 2512 3367 4202 4298 5079 5649 5834 6030 6433
Size: 855 835 96 781 570 185 196 403
Cuts at: 6433 7322 8951 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 855 835 781 570 403
275 250 219 213 196 185 96 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6433 1537
Size: 275 4621 4090
Fragments arranged by size:
4621 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3782 4788 5513 5990 6916 1353
Size: 2429 1006 725 477 926 3423
Fragments arranged by size:
3423 2429 1006 926 725 477
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7573 7947 8093 387
Size: 1064 138 5984 374 146 1280
Fragments arranged by size:
5984 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2270 2573 2641 2720 3272 3353
Size: 393 470 168 303 68 79 552 81
Cuts at: 3353 3887 3935 3944 4355 4509 4538 4584 5051
Size: 534 48 9 411 154 29 46 467
Cuts at: 5051 5427 5501 5773 6209 7049 7646 8433 8701
Size: 376 74 272 436 840 597 787 268
Cuts at: 8701 1239
Size: 1524
Fragments arranged by size:
1524 840 787 597 552 534 470 467
436 411 393 376 303 272 268 168
154 81 79 74 68 48 46 29
9
EcoNI CCTnn'n_nnAGG
Cuts at: 2977 3866 3902 4139 4704 5581 5669 2977
Size: 889 36 237 565 877 88 6294
Fragments arranged by size:
6294 889 877 565 237 88 36
EcoO109I rG'GnC_Cy
Cuts at: 2090 2270 2353 2854 3004 3176 3519 3555 3944
Size: 180 83 501 150 172 343 36 389
Cuts at: 3944 4083 4368 4538 5388 5500 5547 5919 6253
Size: 139 285 170 850 112 47 372 334
Cuts at: 6253 2090
Size: 4823
Fragments arranged by size:
4823 850 501 389 372 343 334 285
180 172 170 150 139 112 83 47
36
EcoRI G'AATT_C
Cuts at: 6295 6673 6295
Size: 378 8608
FspI TGC'GCA
Cuts at: 2901 7492 2901
Size: 4591 4395
HaeII r_GCGC'y
Cuts at: 1678 2311 2766 2925 3163 3399 3811 4272 4391
Size: 633 455 159 238 236 412 461 119
Cuts at: 4391 5177 5287 5324 5827 6048 8133 1678
Size: 786 110 37 503 221 2085 2531
Fragments arranged by size:
2531 2085 786 633 503 461 455 412
238 236 221 159 119 110 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3201 3233 3326 3358 3699
Size: 32 135 32 1629 32 93 32 341
Cuts at: 3699 3731 4579 4610 4611 4642 4660 4692 6245
Size: 32 848 31 1 31 18 32 1553
Cuts at: 6245 6277 7627 7659 7701 7733 1373
Size: 32 1350 32 42 32 2626
Fragments arranged by size:
2626 1629 1553 1350 848 341 135 93
42 32 32 32 32 32 32 32
32 32 31 31 18 1
HincII GTy'rAC
Cuts at: 669 1657 1938 6330 6576 669
Size: 988 281 4392 246 3079
Fragments arranged by size:
4392 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8986
HpaI GTT'AAC
Cuts at: 6576 6576
Size: 8986
KasI G'GCGC_C
Cuts at: 2762 3395 5320 2762
Size: 633 1925 6428
Fragments arranged by size:
6428 1925 633
KpnI G_GTAC'C
Cuts at: 6242 6311 6242
Size: 69 8917
MfeI C'AATT_G
Cuts at: 6585 6585
Size: 8986
MluI A'CGCG_T
Cuts at: 8862 8862
Size: 8986
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4956 5757 8100 8284 1305
Size: 3651 801 2343 184 2007
Fragments arranged by size:
3651 2343 2007 801 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2514 4204 5651 6032 10
Size: 54 1425 343 682 1690 1447 381 2964
Fragments arranged by size:
2964 1690 1447 1425 682 381 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3414 4198 4617 4690 5047 5246 5615
Size: 648 2248 784 419 73 357 199 369
Cuts at: 5615 5810 5993 6038 6944 7303 7462 518
Size: 195 183 45 906 359 159 2042
Fragments arranged by size:
2248 2042 906 784 648 419 369 359
357 199 195 183 159 73 45
MspA1I CmG'CkG
Cuts at: 1606 2596 2724 2739 2812 2863 2882 3028 3042
Size: 990 128 15 73 51 19 146 14
Cuts at: 3042 3331 3376 3582 3695 4073 4131 4382 4460
Size: 289 45 206 113 378 58 251 78
Cuts at: 4460 5085 5229 5333 5438 6402 7079 8227 8472
Size: 625 144 104 105 964 677 1148 245
Cuts at: 8472 1606
Size: 2120
Fragments arranged by size:
2120 1148 990 964 677 625 378 289
251 245 206 146 144 128 113 105
104 78 73 58 51 45 19 15
14
NaeI GCC'GGC
Cuts at: 2779 2807 4829 5288 5744 5981 2779
Size: 28 2022 459 456 237 5784
Fragments arranged by size:
5784 2022 459 456 237 28
NarI GG'CG_CC
Cuts at: 2763 3396 5321 2763
Size: 633 1925 6428
Fragments arranged by size:
6428 1925 633
NcoI C'CATG_G
Cuts at: 513 1065 4652 4945 6118 513
Size: 552 3587 293 1173 3381
Fragments arranged by size:
3587 3381 1173 552 293
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8986
NgoMIV G'CCGG_C
Cuts at: 2777 2805 4827 5286 5742 5979 2777
Size: 28 2022 459 456 237 5784
Fragments arranged by size:
5784 2022 459 456 237 28
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8986
NotI GC'GGCC_GC
Cuts at: 6433 6433
Size: 8986
NspI r_CATG'y
Cuts at: 1132 1229 3273 3463 5237 5620 5728 5908 6344
Size: 97 2044 190 1774 383 108 180 436
Cuts at: 6344 7889 1132
Size: 1545 2229
Fragments arranged by size:
2229 2044 1774 1545 436 383 190 180
108 97
PasI CC'CwG_GG
Cuts at: 1348 2362 4321 5627 5922 1348
Size: 1014 1959 1306 295 4412
Fragments arranged by size:
4412 1959 1306 1014 295
PciI A'CATG_T
Cuts at: 3459 5724 7885 3459
Size: 2265 2161 4560
Fragments arranged by size:
4560 2265 2161
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5146 5843 6028 1108
Size: 4038 697 185 4066
Fragments arranged by size:
4066 4038 697 185
PfoI T'CCnGG_A
Cuts at: 3066 3864 3951 4788 5443 6084 6263 3066
Size: 798 87 837 655 641 179 5789
Fragments arranged by size:
5789 837 798 655 641 179 87
PmeI GTTT'AAAC
Cuts at: 6290 6290
Size: 8986
PmlI CAC'GTG
Cuts at: 2746 3666 4690 2746
Size: 920 1024 7042
Fragments arranged by size:
7042 1024 920
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6155 6187 7055 7087 8568 8600 6155
Size: 32 868 32 1481 32 6541
Fragments arranged by size:
6541 1481 868 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2090 2353 2854 3176 3519 3555 3944 6253 2090
Size: 263 501 322 343 36 389 2309 4823
Fragments arranged by size:
4823 2309 501 389 343 322 263 36
PshAI GACnn'nnGTC
Cuts at: 1613 3749 1613
Size: 2136 6850
PsiI TTA'TAA
Cuts at: 6556 6556
Size: 8986
PspOMI G'GGCC_C
Cuts at: 2269 4082 4368 4941 5387 5546 5918 2269
Size: 1813 286 573 446 159 372 5337
Fragments arranged by size:
5337 1813 573 446 372 286 159
PstI C_TGCA'G
Cuts at: 830 2233 2801 3047 3587 4633 4892 5027 5714
Size: 1403 568 246 540 1046 259 135 687
Cuts at: 5714 6338 830
Size: 624 3478
Fragments arranged by size:
3478 1403 1046 687 624 568 540 259
246 135
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8986
PvuII CAG'CTG
Cuts at: 2596 2812 3042 3331 3582 3695 4131 4382 4460
Size: 216 230 289 251 113 436 251 78
Cuts at: 4460 5085 5333 2596
Size: 625 248 6249
Fragments arranged by size:
6249 625 436 289 251 251 248 230
216 113 78
SacII CC_GC'GG
Cuts at: 2864 2883 2864
Size: 19 8967
SalI G'TCGA_C
Cuts at: 1936 6328 1936
Size: 4392 4594
SanDI GG'GwC_CC
Cuts at: 2090 2353 2854 3176 3519 3555 3944 2090
Size: 263 501 322 343 36 389 7132
Fragments arranged by size:
7132 501 389 343 322 263 36
SapI GCTCTTCn'nnn_
Cuts at: 4788 5513 5990 4788
Size: 725 477 7784
Fragments arranged by size:
7784 725 477
SbfI CC_TGCA'GG
Cuts at: 6338 6338
Size: 8986
ScaI AGT'ACT
Cuts at: 6218 7234 8946 6218
Size: 1016 1712 6258
Fragments arranged by size:
6258 1712 1016
SexAI A'CCwGG_T
Cuts at: 2385 2523 2385
Size: 138 8848
SfcI C'TryA_G
Cuts at: 826 1045 2229 2797 2917 3043 3583 4126 4629
Size: 219 1184 568 120 126 540 543 503
Cuts at: 4629 4888 5023 5710 5890 6334 7469 8150 8341
Size: 259 135 687 180 444 1135 681 191
Cuts at: 8341 826
Size: 1471
Fragments arranged by size:
1471 1184 1135 687 681 568 543 540
503 444 259 219 191 180 135 126
120
SfoI GGC'GCC
Cuts at: 2764 3397 5322 2764
Size: 633 1925 6428
Fragments arranged by size:
6428 1925 633
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8986
SgrAI Cr'CCGG_yG
Cuts at: 1700 5286 1700
Size: 3586 5400
SmaI CCC'GGG
Cuts at: 1872 2024 2875 3545 4940 5498 5545 6313 1872
Size: 152 851 670 1395 558 47 768 4545
Fragments arranged by size:
4545 1395 851 768 670 558 152 47
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2152 4677 5594 5693 6100 7090
Size: 197 926 209 2525 917 99 407 990
Cuts at: 7090 7991 8253 820
Size: 901 262 1553
Fragments arranged by size:
2525 1553 990 926 917 901 407 262
209 197 99
SnaBI TAC'GTA
Cuts at: 493 6777 493
Size: 6284 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8986
SphI G_CATG'C
Cuts at: 1132 6344 1132
Size: 5212 3774
SrfI GCCC'GGGC
Cuts at: 4940 5545 4940
Size: 605 8381
SspI AAT'ATT
Cuts at: 5 52 6910 5
Size: 47 6858 2081
Fragments arranged by size:
6858 2081 47
StuI AGG'CCT
Cuts at: 1517 3314 3772 4058 4211 4473 5021 1517
Size: 1797 458 286 153 262 548 5482
Fragments arranged by size:
5482 1797 548 458 286 262 153
StyI C'CwwG_G
Cuts at: 513 1065 2955 3105 3436 4177 4479 4652 4945
Size: 552 1890 150 331 741 302 173 293
Cuts at: 4945 5045 5065 5092 5134 6118 6425 513
Size: 100 20 27 42 984 307 3074
Fragments arranged by size:
3074 1890 984 741 552 331 307 302
293 173 150 100 42 27 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2460 2606 3377 4051 4997 6068 6107
Size: 1255 880 146 771 674 946 1071 39
Cuts at: 6107 7013 7030 7183 7368 7813 325
Size: 906 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1071 946 906 880 771 674
445 185 153 146 39 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3885 4872 6216
Size: 274 80 33 51 1443 1908 987 1344
Cuts at: 6216 7232 8944 96
Size: 1016 1712 138
Fragments arranged by size:
1908 1712 1443 1344 1016 987 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3067 3083 3099 3115 3198 3230 3434 3466 4784
Size: 16 16 16 83 32 204 32 1318
Cuts at: 4784 4816 4970 5002 5832 5864 8946 8978 3067
Size: 32 154 32 830 32 3082 32 3075
Fragments arranged by size:
3082 3075 1318 830 204 154 83 32
32 32 32 32 32 16 16 16
Tth111I GACn'n_nGTC
Cuts at: 1316 2525 1316
Size: 1209 7777
XbaI T'CTAG_A
Cuts at: 6322 6322
Size: 8986
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2986 3647 4056 4153 5809 6025 2986
Size: 661 409 97 1656 216 5947
Fragments arranged by size:
5947 1656 661 409 216 97
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8986
XmaI C'CCGG_G
Cuts at: 1870 2022 2873 3543 4938 5496 5543 6311 1870
Size: 152 851 670 1395 558 47 768 4545
Fragments arranged by size:
4545 1395 851 768 670 558 152 47
XmnI GAAnn'nnTTC
Cuts at: 2346 7115 2346
Size: 4769 4217
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6794 276
Size: 53 83 186 936 5260 2468
Fragments arranged by size:
5260 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI BaeI BbvCI BfrBI BsiWI BstZ17I EcoICRI EcoRV
FalI FseI FspAI NruI NsiI PacI PspXI PsrI
RsrII SacI SfiI SwaI