################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccI AclI AfaI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BamHI BanI BbeI BbrPI BcgI BciVI BclI BfaI BfrI BlnI BsaAI BsaBI BsaI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrGI BsrI BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KasI KpnI MaeI MaeII MamI MfeI MluI MseI MslI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SalI SapI ScaI SexAI SfoI SfuI SnaBI SpeI SphI SspBI SspI SwaI TaqI TfiI Tru9I Tth111I TthHB8I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccIII 164 TCCGGA T^CCGGA AcsI 160 AAATTC [GA]^AATT[CT] AcyI 22 GACGCC G[GA]^CG[CT]C AocI 247 CCTGAGG CC^T[ACGT]AGG ApaI 124 GGGCCC GGGCC^C ApoI 160 AAATTC [GA]^AATT[CT] AspHI 190 GAGCAC G[AT]GC[AT]^C AviII 986 TGCGCA TGC^GCA BbsI 619 GAAGAC GAAGAC(2/6) BbvCI 511 GCTGAGG CCTCAGC(-5/-2) BglII 50 AGATCT A^GATCT BpuAI 619 GAAGAC GAAGAC(2/6) BsaHI 22 GACGCC G[GA]^CG[CT]C BseAI 164 TCCGGA T^CCGGA BsgI 564 CTGCAC GTGCAG(16/14) BsiEI 932 CGGCCG CG[GA][CT]^CG BsiHKAI 190 GAGCAC G[AT]GC[AT]^C BspEI 164 TCCGGA T^CCGGA BstAPI 978 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 200 CCAAGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 247 CCTGAGG CC^T[ACGT]AGG EagI 932 CGGCCG C^GGCCG EclXI 932 CGGCCG C^GGCCG Eco52I 932 CGGCCG C^GGCCG Eco81I 247 CCTGAGG CC^T[ACGT]AGG FspI 986 TGCGCA TGC^GCA HgaI 22 GACGC GACGC(5/10) Hin1I 22 GACGCC G[GA]^CG[CT]C HinfI 325 GAGTC G^A[ACGT]TC KspI 574 CCGCGG CCGC^GG MaeIII 848 GTGAC ^GT[ACGT]AC MroI 164 TCCGGA T^CCGGA NlaIII 364 CATG CATG^ PflMI 531 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 325 GAGTC GAGTC(4/5) PpuMI 1058 AGGACCT [GA]G^G[AT]CC[CT] PvuII 712 CAGCTG CAG^CTG SacII 574 CCGCGG CCGC^GG SbfI 726 CCTGCAGG CCTGCA^GG SfiI 519 GGCCACGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 913 CGCCGGTG C[GA]^CCGG[CT]G SmlI 104 CTCAAG C^T[CT][GA]AG Sse8387I 726 CCTGCAGG CCTGCA^GG Tsp45I 848 GTGAC ^GT[GC]AC Tsp509I 161 AATT ^AATT Van91I 531 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AlwI 372 GATCC GGATC(4/5) 843 GATCC AvaI 383 CCCGGG C^[CT]CG[GA]G 586 CCCGGG BalI 41 TGGCCA TGG^CCA 311 TGGCCA BanII 124 GGGCCC G[GA]GC[CT]^C 1028 GAGCCC BlpI 187 GCTGAGC GC^T[ACGT]AGC 284 GCTCAGC Bpu1102I 187 GCTGAGC GC^T[ACGT]AGC 284 GCTCAGC BsaWI 164 TCCGGA [AT]^CCGG[AT] 458 ACCGGA BseRI 817 CTCCTC GAGGAG(10/8) 514 GAGGAG BsmFI 455 GGGAC GGGAC(10/14) 411 GTCCC BsoBI 383 CCCGGG C^[CT]CG[GA]G 586 CCCGGG BspMI 29 ACCTGC ACCTGC(4/8) 983 ACCTGC BssHII 82 GCGCGC G^CGCGC 1068 GCGCGC CelII 187 GCTGAGC GC^T[ACGT]AGC 284 GCTCAGC DsaI 521 CCACGG C^C[GA][CT]GG 574 CCGCGG HphI 612 GGTGA GGTGA(8/7) 669 GGTGA MluNI 41 TGGCCA TGG^CCA 311 TGGCCA MscI 41 TGGCCA TGG^CCA 311 TGGCCA SfaNI 951 GATGC GCATC(5/9) 1046 GCATC SmaI 383 CCCGGG CCC^GGG 586 CCCGGG StuI 244 AGGCCT AGG^CCT 1074 AGGCCT TspRI 315 CACTG CA[GC]TG[ACGT][ACGT]^ 396 CAGTG XmaCI 383 CCCGGG C^CCGGG 586 CCCGGG XmaI 383 CCCGGG C^CCGGG 586 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AccII AciI AluI AlwNI AvaII BbvI BcnI BglI BmyI BpmI BsaJI BsiYI BslI Bsp1286I BsrFI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI Ksp632I MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIV PstI Sau3AI Sau96I ScrFI SfcI StyI TseI XhoII