(Linear) (Six-base) MAPSORT of: KIEE5540.seq Check: 688 from: 1 to: 1083 KIEE5540.orf With 182 enzymes: SgfI * January 29, 2014 12:04 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 898 1083 Size: 898 185 AlwNI CAG_nnn'CTG Cuts at: 0 39 118 232 360 717 789 984 1062 Size: 39 79 114 128 357 72 195 78 Cuts at: 1062 1083 Size: 21 Fragments arranged by size: 357 195 128 114 79 78 72 39 21 ApaI G_GGCC'C Cuts at: 0 128 1083 Size: 128 955 ApoI r'AATT_y Cuts at: 0 160 1083 Size: 160 923 AvaI C'yCGr_G Cuts at: 0 383 586 1083 Size: 383 203 497 Fragments arranged by size: 497 383 203 BanII G_rGCy'C Cuts at: 0 128 1032 1083 Size: 128 904 51 Fragments arranged by size: 904 128 51 BbsI GAAGACnn'nnnn_ Cuts at: 0 626 1083 Size: 626 457 BbvCI CC'TCA_GC Cuts at: 0 512 1083 Size: 512 571 BglI GCCn_nnn'nGGC Cuts at: 0 235 508 526 588 637 1083 Size: 235 273 18 62 49 446 Fragments arranged by size: 446 273 235 62 49 18 BglII A'GATC_T Cuts at: 0 50 1083 Size: 50 1033 BlpI GC'TnA_GC Cuts at: 0 188 285 1083 Size: 188 97 798 Fragments arranged by size: 798 188 97 Bme1580I G_kGCm'C Cuts at: 0 128 1083 Size: 128 955 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 206 739 811 1083 Size: 206 533 72 272 Fragments arranged by size: 533 272 206 72 Bpu10I CC'TnA_GC Cuts at: 0 512 1083 Size: 512 571 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 89 1083 Size: 89 994 BsaHI Gr'CG_yC Cuts at: 0 23 1083 Size: 23 1060 BsaWI w'CCGG_w Cuts at: 0 164 458 1083 Size: 164 294 625 Fragments arranged by size: 625 294 164 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 529 808 1083 Size: 529 279 275 Fragments arranged by size: 529 279 275 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 549 1083 Size: 549 534 BsiEI CG_ry'CG Cuts at: 0 935 1083 Size: 935 148 BsiHKAI G_wGCw'C Cuts at: 0 194 1083 Size: 194 889 Bsp1286I G_dGCh'C Cuts at: 0 128 194 1032 1083 Size: 128 66 838 51 Fragments arranged by size: 838 128 66 51 BspEI T'CCGG_A Cuts at: 0 164 1083 Size: 164 919 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 38 992 1083 Size: 38 954 91 Fragments arranged by size: 954 91 38 BsrFI r'CCGG_y Cuts at: 0 627 707 743 914 934 968 1083 Size: 627 80 36 171 20 34 115 Fragments arranged by size: 627 171 115 80 36 34 20 BssHII G'CGCG_C Cuts at: 0 82 1068 1083 Size: 82 986 15 Fragments arranged by size: 986 82 15 BstAPI GCAn_nnn'nTGC Cuts at: 0 984 1083 Size: 984 99 BstXI CCAn_nnnn'nTGG Cuts at: 0 207 1083 Size: 207 876 BstYI r'GATC_y Cuts at: 0 50 371 842 1083 Size: 50 321 471 241 Fragments arranged by size: 471 321 241 50 Bsu36I CC'TnA_GG Cuts at: 0 248 1083 Size: 248 835 BtgI C'CryG_G Cuts at: 0 521 574 1083 Size: 521 53 509 Fragments arranged by size: 521 509 53 BtsI GCAGTG_nn' Cuts at: 0 402 1083 Size: 402 681 EaeI y'GGCC_r Cuts at: 0 41 311 629 762 932 1083 Size: 41 270 318 133 170 151 Fragments arranged by size: 318 270 170 151 133 41 EagI C'GGCC_G Cuts at: 0 932 1083 Size: 932 151 EarI CTCTTCn'nnn_ Cuts at: 0 170 460 652 1083 Size: 170 290 192 431 Fragments arranged by size: 431 290 192 170 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 453 844 1083 Size: 453 391 239 Fragments arranged by size: 453 391 239 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 206 739 811 898 1083 Size: 206 533 72 87 185 Fragments arranged by size: 533 206 185 87 72 EcoNI CCTnn'n_nnAGG Cuts at: 0 823 865 1054 1083 Size: 823 42 189 29 Fragments arranged by size: 823 189 42 29 EcoO109I rG'GnC_Cy Cuts at: 0 124 125 560 873 1059 1083 Size: 124 1 435 313 186 24 Fragments arranged by size: 435 313 186 124 24 1 FspI TGC'GCA Cuts at: 0 988 1083 Size: 988 95 HaeII r_GCGC'y Cuts at: 0 726 915 963 1017 1083 Size: 726 189 48 54 66 Fragments arranged by size: 726 189 66 54 48 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 310 342 1083 Size: 310 32 741 Fragments arranged by size: 741 310 32 MscI TGG'CCA Cuts at: 0 43 313 1083 Size: 43 270 770 Fragments arranged by size: 770 270 43 MspA1I CmG'CkG Cuts at: 0 576 690 714 906 1083 Size: 576 114 24 192 177 Fragments arranged by size: 576 192 177 114 24 NaeI GCC'GGC Cuts at: 0 629 745 936 1083 Size: 629 116 191 147 Fragments arranged by size: 629 191 147 116 NgoMIV G'CCGG_C Cuts at: 0 627 743 934 1083 Size: 627 116 191 149 Fragments arranged by size: 627 191 149 116 PflMI CCAn_nnn'nTGG Cuts at: 0 537 1083 Size: 537 546 PfoI T'CCnGG_A Cuts at: 0 374 1083 Size: 374 709 PpuMI rG'GwC_Cy Cuts at: 0 1059 1083 Size: 1059 24 PspOMI G'GGCC_C Cuts at: 0 124 1083 Size: 124 959 PstI C_TGCA'G Cuts at: 0 35 123 303 731 869 1083 Size: 35 88 180 428 138 214 Fragments arranged by size: 428 214 180 138 88 35 PvuII CAG'CTG Cuts at: 0 714 1083 Size: 714 369 SacII CC_GC'GG Cuts at: 0 577 1083 Size: 577 506 SbfI CC_TGCA'GG Cuts at: 0 731 1083 Size: 731 352 SfcI C'TryA_G Cuts at: 0 31 119 299 727 865 1083 Size: 31 88 180 428 138 218 Fragments arranged by size: 428 218 180 138 88 31 SfiI GGCCn_nnn'nGGCC Cuts at: 0 526 1083 Size: 526 557 SgrAI Cr'CCGG_yG Cuts at: 0 914 1083 Size: 914 169 SmaI CCC'GGG Cuts at: 0 385 588 1083 Size: 385 203 495 Fragments arranged by size: 495 385 203 SmlI C'TyrA_G Cuts at: 0 104 1083 Size: 104 979 SrfI GCCC'GGGC Cuts at: 0 588 1083 Size: 588 495 StuI AGG'CCT Cuts at: 0 246 1076 1083 Size: 246 830 7 Fragments arranged by size: 830 246 7 StyI C'CwwG_G Cuts at: 0 17 206 401 422 1083 Size: 17 189 195 21 661 Fragments arranged by size: 661 195 189 21 17 XmaI C'CCGG_G Cuts at: 0 383 586 1083 Size: 383 203 497 Fragments arranged by size: 497 383 203 Enzymes that do cut: AcuI AlwNI ApaI ApoI AvaI BanII BbsI BbvCI BglI BglII BlpI Bme1580I BpmI Bpu10I BpuEI BsaHI BsaWI BseRI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrFI BssHII BstAPI BstXI BstYI Bsu36I BtgI BtsI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I MscI MspA1I NaeI NgoMIV PflMI PfoI PpuMI PspOMI PstI PvuII SacII SbfI SfcI SfiI SgrAI SmaI SmlI SrfI StuI StyI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI BfrBI BmgBI BmrI BmtI BplI BsaI BsaAI BsaBI BsaXI BseYI BsiWI BsmI BsmBI BspHI BsrBI BsrDI BsrGI BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MslI NarI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI ScaI SexAI SfoI SgfI SnaBI SpeI SphI SspI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI