################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI Asp700I AspEI AviII AvrII BbeI BbrPI BbvCI BclI BcnI BfaI BfrI BglII BlnI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmI BspDI BspEI BspLU11I BsrFI BssSI Bst1107I BstEII BstPI BstXI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HapII HincII HindII HindIII HpaI HpaII KasI MaeI MamI MfeI MroI MspI MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI SwaI XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 742 GACGTC GACGT^C Acc65I 1202 GGTACC G^GTACC AocI 200 CCTCAGG CC^T[ACGT]AGG AseI 1411 ATTAAT AT^TAAT AsnI 1411 ATTAAT AT^TAAT Asp718I 1202 GGTACC G^GTACC AspI 502 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 580 TGGCCA TGG^CCA BamHI 449 GGATCC G^GATCC BanII 943 GAGCTC G[GA]GC[CT]^C BcgI 806 CGAGGGTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 26 GTATCC GTATCC(6/5) BglI 856 GCCCTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1005 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1005 GCTGAGC GC^T[ACGT]AGC Bsp1407I 1214 TGTACA T^GTACA BsrBI 1159 GAGCGG CCGCTC(-3/-3) BsrDI 220 GCAATG GCAATG(2/0) BsrGI 1214 TGTACA T^GTACA BssHII 344 GCGCGC G^CGCGC BstAPI 81 GCAGAACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1387 TTCGAA TT^CGAA Bsu36I 200 CCTCAGG CC^T[ACGT]AGG CelII 1005 GCTGAGC GC^T[ACGT]AGC Eco81I 200 CCTCAGG CC^T[ACGT]AGG EcoT14I 1232 CCATGG C^C[AT][AT]GG HaeII 1013 AGCGCC [GA]GCGC^[CT] KpnI 1202 GGTACC GGTAC^C KspI 1066 CCGCGG CCGC^GG MaeIII 1189 GTCAC ^GT[ACGT]AC MluI 430 ACGCGT A^CGCGT MluNI 580 TGGCCA TGG^CCA MscI 580 TGGCCA TGG^CCA MslI 996 CATGAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 1232 CCATGG C^CATGG NspI 86 ACATGC [GA]CATG^[CT] NspV 1387 TTCGAA TT^CGAA PflFI 502 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 1167 GACTC GAGTC(4/5) PshAI 762 GACCTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1411 ATTAAT AT^TAAT SacI 943 GAGCTC GAGCT^C SacII 1066 CCGCGG CCGC^GG SapI 1276 GAAGAGC GCTCTTC(1/4) SfuI 1387 TTCGAA TT^CGAA SspBI 1214 TGTACA T^GTACA SspI 183 AATATT AAT^ATT StyI 1232 CCATGG C^C[AT][AT]GG Tsp45I 1189 GTCAC ^GT[GC]AC Tsp509I 1179 AATT ^AATT Tth111I 502 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AflIII 430 ACGCGT A^C[GA][CT]GT 477 ACACGT AspHI 494 GTGCTC G[AT]GC[AT]^C 943 GAGCTC BanI 615 GGTGCC G^G[CT][GA]CC 1202 GGTACC BsiHKAI 494 GTGCTC G[AT]GC[AT]^C 943 GAGCTC BspHI 134 TCATGA T^CATGA 995 TCATGA BspMI 1088 ACCTGC ACCTGC(4/8) 1281 GCAGGT DraII 1070 GGGACCT [GA]G^G[ACGT]CC[CT] 1370 AGGACCC DsaI 1066 CCGCGG C^C[GA][CT]GG 1232 CCATGG EcoNI 993 CCTCATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1074 CCTCGACCAGG EcoO109I 1070 GGGACCT [GA]G^G[ACGT]CC[CT] 1370 AGGACCC MseI 277 TTAA T^TAA 1412 TTAA PpuMI 1070 GGGACCT [GA]G^G[AT]CC[CT] 1370 AGGACCC PstI 847 CTGCAG CTGCA^G 1255 CTGCAG PvuII 358 CAGCTG CAG^CTG 1319 CAGCTG RcaI 134 TCATGA T^CATGA 995 TCATGA Tru9I 277 TTAA T^TAA 1412 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AvaI AvaII BbsI BbvI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII EaeI EarI EcoRII FauI Fnu4HI FokI HaeIII HgaI HhaI Hin1I HinP1I HinfI HphI ItaI Ksp632I MaeII MboI MboII MflI MnlI MspA1I MvaI MvnI MwoI NdeII NlaIII NlaIV PflMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI TseI TspRI TthHB8I Van91I XhoII