Restriction map for
pFN21AE3211
(Circular) (Six-base) MAPSORT of: pFN21AE3211.seq Check: 7891 from: 1 to: 6142
pFN21AE3211 6142 bp
With 182 enzymes: SgfI *
March 28, 2012 12:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3102 3102
Size: 6142
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2751 3952 278
Size: 53 83 186 936 1215 1201 2468
Fragments arranged by size:
2468 1215 1201 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3485 1937
Size: 1548 4594
Acc65I G'GTAC_C
Cuts at: 3207 3463 3207
Size: 256 5886
AclI AA'CG_TT
Cuts at: 1563 4269 4642 1563
Size: 2706 373 3063
Fragments arranged by size:
3063 2706 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2406 3300 4205 5589 5857 1632
Size: 774 894 905 1384 268 1917
Fragments arranged by size:
1917 1384 905 894 774 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5945
AflIII A'CryG_T
Cuts at: 1203 2435 2482 5041 6018 1203
Size: 1232 47 2559 977 1327
Fragments arranged by size:
2559 1327 1232 977 47
AgeI A'CCGG_T
Cuts at: 4973 4973
Size: 6142
AhdI GACnn_n'nnGTC
Cuts at: 4871 4871
Size: 6142
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6142
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6142
AlwNI CAG_nnn'CTG
Cuts at: 2449 2464 2659 2764 2987 3022 3197 3885 5457
Size: 15 195 105 223 35 175 688 1572
Cuts at: 5457 2449
Size: 3134
Fragments arranged by size:
3134 1572 688 223 195 175 105 35
15
ApaLI G'TGCA_C
Cuts at: 4199 5355 4199
Size: 1156 4986
ApoI r'AATT_y
Cuts at: 1493 3451 3677 3829 5938 1493
Size: 1958 226 152 2109 1697
Fragments arranged by size:
2109 1958 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3417 4696 160
Size: 872 2385 1279 1606
Fragments arranged by size:
2385 1606 1279 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
AvaI C'yCGr_G
Cuts at: 1870 1943 2039 2809 2825 3467 1870
Size: 73 96 770 16 642 4545
Fragments arranged by size:
4545 770 642 96 73 16
BamHI G'GATC_C
Cuts at: 2454 3472 3839 2454
Size: 1018 367 4757
Fragments arranged by size:
4757 1018 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2620 3207 3463 4918 618
Size: 325 217 627 833 587 256 1455 1842
Fragments arranged by size:
1842 1455 833 627 587 325 256 217
BanII G_rGCy'C
Cuts at: 1391 2952 3461 1391
Size: 1561 509 4072
Fragments arranged by size:
4072 1561 509
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2342 2743 3382 928
Size: 971 443 401 639 3688
Fragments arranged by size:
3688 971 639 443 401
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2800 2834 4333 4367 1903
Size: 34 863 34 1499 34 3678
Fragments arranged by size:
3678 1499 863 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2042 4034 5244 2042
Size: 1992 1210 2940
Fragments arranged by size:
2940 1992 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6142
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2867 4753 136
Size: 107 122 71 631 605 1195 1886 1525
Fragments arranged by size:
1886 1525 1195 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6137 6137
Size: 6142
BlpI GC'TnA_GC
Cuts at: 3011 3559 3011
Size: 548 5594
Bme1580I G_kGCm'C
Cuts at: 1790 2178 4203 5359 1790
Size: 388 2025 1156 2573
Fragments arranged by size:
2573 2025 1156 388
BmgBI CAC'GTC
Cuts at: 1318 2485 1318
Size: 1167 4975
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2044 3207 3873
Size: 416 53 83 399 24 627 1163 666
Cuts at: 3873 4831 442
Size: 958 1753
Fragments arranged by size:
1753 1163 958 666 627 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6142
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2650 2650
Size: 6142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2117 2288 2487 2577 2663 2684 4802 1239
Size: 878 171 199 90 86 21 2118 2579
Fragments arranged by size:
2579 2118 878 199 171 90 86 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4267 5132 5430 4267
Size: 865 298 4979
Fragments arranged by size:
4979 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4805 882
Size: 617 3306 2219
Fragments arranged by size:
3306 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3933 493
Size: 711 2729 2702
Fragments arranged by size:
2729 2702 711
BsaBI GATnn'nnATC
Cuts at: 6136 6136
Size: 6142
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2010 2748 3066 3949
Size: 53 83 186 936 477 738 318 883
Cuts at: 3949 4331 4966 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 883 738 635 477 382 318
186 83 53
BsaWI w'CCGG_w
Cuts at: 4575 4973 5247 5394 5805 4575
Size: 398 274 147 411 4912
Fragments arranged by size:
4912 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3326 3356 1178
Size: 30 165 30 1923 30 3964
Fragments arranged by size:
3964 1923 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2075 2249 2987 1186
Size: 889 174 738 4341
Fragments arranged by size:
4341 889 738 174
BseYI C'CCAG_C
Cuts at: 2345 3051 5345 5841 2345
Size: 706 2294 496 2646
Fragments arranged by size:
2646 2294 706 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5569
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3592 4353 5381 1540
Size: 275 186 1591 761 1028 2301
Fragments arranged by size:
2301 1591 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2503 2952 4203 4288 5359 2503
Size: 449 1251 85 1071 3286
Fragments arranged by size:
3286 1251 1071 449 85
BsmI GAATG_Cn'
Cuts at: 3653 3746 3653
Size: 93 6049
BsmBI CGTCTCn'nnnn_
Cuts at: 2426 2618 2639 2426
Size: 192 21 5929
Fragments arranged by size:
5929 192 21
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2178 2503 2952 3461 4203 4288 5359
Size: 399 388 325 449 509 742 85 1071
Cuts at: 5359 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 509 449 399 388 325
85
BspEI T'CCGG_A
Cuts at: 5805 5805
Size: 6142
BspHI T'CATG_A
Cuts at: 2139 3000 4031 5761 2139
Size: 861 1031 1730 2520
Fragments arranged by size:
2520 1730 1031 861
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3102 3277 3497 844
Size: 2258 175 220 3489
Fragments arranged by size:
3489 2258 220 175
BsrBI CCG'CTC
Cuts at: 1394 1823 3166 4029 1394
Size: 429 1343 863 3507
Fragments arranged by size:
3507 1343 863 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2232 4637 4811 65
Size: 1183 984 2405 174 1396
Fragments arranged by size:
2405 1396 1183 984 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4786 4973 1700
Size: 110 2976 187 2869
Fragments arranged by size:
2976 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3219 96
Size: 3123 3019
BssHII G'CGCG_C
Cuts at: 1925 2349 1925
Size: 424 5718
BssSI C'ACGA_G
Cuts at: 4202 5214 4202
Size: 1012 5130
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2092 794
Size: 1298 4844
BstBI TT'CG_AA
Cuts at: 3393 4946 3393
Size: 1553 4589
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6142
BstYI r'GATC_y
Cuts at: 1972 2125 2454 3243 3472 3839 4224 4241 5693
Size: 153 329 789 229 367 385 17 1452
Cuts at: 5693 5779 5791 6137 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 789 385 367 346 329 229
153 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2206 2206
Size: 6142
BtgI C'CryG_G
Cuts at: 513 1065 2004 3071 3237 513
Size: 552 939 1067 166 3418
Fragments arranged by size:
3418 1067 939 552 166
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5403
Fragments arranged by size:
5403 683 56
BtsI GCAGTG_nn'
Cuts at: 2845 3658 4452 4472 2845
Size: 813 794 20 4515
Fragments arranged by size:
4515 813 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6142
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6107
DraI TTT'AAA
Cuts at: 3446 3446
Size: 6142
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5149 809
Size: 507 3833 1802
Fragments arranged by size:
3833 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2301 2585 3351
Size: 54 1425 50 275 18 471 284 766
Cuts at: 3351 3589 4478 6107 8
Size: 238 889 1629 43
Fragments arranged by size:
1629 1425 889 766 471 284 275 238
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3589 1537
Size: 275 1777 4090
Fragments arranged by size:
4090 1777 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2382 2531 3276 4072 1353
Size: 1029 149 745 796 3423
Fragments arranged by size:
3423 1029 796 745 149
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4729 5103 5249 387
Size: 1064 138 3140 374 146 1280
Fragments arranged by size:
3140 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2950 2950
Size: 6142
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2117 2288 2406 2487 2577 2663 2684
Size: 393 485 171 118 81 90 86 21
Cuts at: 2684 3300 4205 4802 5589 5857 1239
Size: 616 905 597 787 268 1524
Fragments arranged by size:
1524 905 787 616 597 485 393 268
171 118 90 86 81 21
EcoNI CCTnn'n_nnAGG
Cuts at: 3002 3083 3002
Size: 81 6061
EcoO109I rG'GnC_Cy
Cuts at: 3076 3376 3076
Size: 300 5842
EcoRI G'AATT_C
Cuts at: 3451 3829 3451
Size: 378 5764
FspI TGC'GCA
Cuts at: 4648 4648
Size: 6142
HaeII r_GCGC'y
Cuts at: 1678 3022 5289 1678
Size: 1344 2267 2531
Fragments arranged by size:
2531 2267 1344
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3235 3267 3403 3435 4783
Size: 32 135 32 1663 32 136 32 1348
Cuts at: 4783 4815 4857 4889 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1663 1348 136 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3486 3732 669
Size: 988 281 1548 246 3079
Fragments arranged by size:
3079 1548 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6142
HpaI GTT'AAC
Cuts at: 3732 3732
Size: 6142
KpnI G_GTAC'C
Cuts at: 3211 3467 3211
Size: 256 5886
MfeI C'AATT_G
Cuts at: 3741 3741
Size: 6142
MluI A'CGCG_T
Cuts at: 2435 6018 2435
Size: 3583 2559
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5256 5440 1305
Size: 3951 184 2007
Fragments arranged by size:
3951 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2587 10
Size: 54 1425 343 755 3565
Fragments arranged by size:
3565 1425 755 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3005 4100 4459 4618 518
Size: 648 1839 1095 359 159 2042
Fragments arranged by size:
2042 1839 1095 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2365 3073 3326 3558 4235 5383 5628 1606
Size: 759 708 253 232 677 1148 245 2120
Fragments arranged by size:
2120 1148 759 708 677 253 245 232
NcoI C'CATG_G
Cuts at: 513 1065 2004 3237 513
Size: 552 939 1233 3418
Fragments arranged by size:
3418 1233 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6142
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6142
NotI GC'GGCC_GC
Cuts at: 3589 3589
Size: 6142
NspI r_CATG'y
Cuts at: 1132 1229 2095 3500 5045 1132
Size: 97 866 1405 1545 2229
Fragments arranged by size:
2229 1545 1405 866 97
PasI CC'CwG_GG
Cuts at: 1348 2464 1348
Size: 1116 5026
PciI A'CATG_T
Cuts at: 5041 5041
Size: 6142
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2464 3022 3197 1108
Size: 1356 558 175 4053
Fragments arranged by size:
4053 1356 558 175
PmeI GTTT'AAAC
Cuts at: 3446 3446
Size: 6142
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4211 4243 5724 5756 4211
Size: 32 1481 32 4597
Fragments arranged by size:
4597 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3076 3376 3076
Size: 300 5842
PshAI GACnn'nnGTC
Cuts at: 1613 2771 1613
Size: 1158 4984
PsiI TTA'TAA
Cuts at: 3712 3712
Size: 6142
PstI C_TGCA'G
Cuts at: 830 2856 3264 3494 830
Size: 2026 408 230 3478
Fragments arranged by size:
3478 2026 408 230
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6142
PvuII CAG'CTG
Cuts at: 2365 3326 2365
Size: 961 5181
SacI G_AGCT'C
Cuts at: 2952 2952
Size: 6142
SacII CC_GC'GG
Cuts at: 3074 3074
Size: 6142
SalI G'TCGA_C
Cuts at: 1936 3484 1936
Size: 1548 4594
SapI GCTCTTCn'nnn_
Cuts at: 3276 3276
Size: 6142
SbfI CC_TGCA'GG
Cuts at: 3494 3494
Size: 6142
ScaI AGT'ACT
Cuts at: 4390 6102 4390
Size: 1712 4430
SfcI C'TryA_G
Cuts at: 826 1045 2852 3260 3313 3490 4625 5306 5497
Size: 219 1807 408 53 177 1135 681 191
Cuts at: 5497 826
Size: 1471
Fragments arranged by size:
1807 1471 1135 681 408 219 191 177
53
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6142
SmaI CCC'GGG
Cuts at: 1872 3469 1872
Size: 1597 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4246 5147 5409 820
Size: 197 926 2303 901 262 1553
Fragments arranged by size:
2303 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3933 493
Size: 3440 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6142
SphI G_CATG'C
Cuts at: 1132 3500 1132
Size: 2368 3774
SspI AAT'ATT
Cuts at: 5 52 2190 4066 5
Size: 47 2138 1876 2081
Fragments arranged by size:
2138 2081 1876 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6142
StyI C'CwwG_G
Cuts at: 513 1065 2004 3237 3581 513
Size: 552 939 1233 344 3074
Fragments arranged by size:
3074 1233 939 552 344
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2491 3043 4169 4186 4339 4524 4969
Size: 1255 911 552 1126 17 153 185 445
Cuts at: 4969 325
Size: 1498
Fragments arranged by size:
1498 1255 1126 911 552 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3219 4388 6100
Size: 274 80 33 51 1443 1242 1169 1712
Cuts at: 6100 96
Size: 138
Fragments arranged by size:
1712 1443 1242 1169 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6102 6134 6102
Size: 32 6110
Tth111I GACn'n_nGTC
Cuts at: 1316 2510 1316
Size: 1194 4948
XbaI T'CTAG_A
Cuts at: 3478 3478
Size: 6142
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6142
XmaI C'CCGG_G
Cuts at: 1870 3467 1870
Size: 1597 4545
XmnI GAAnn'nnTTC
Cuts at: 4271 4271
Size: 6142
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2749 3950 276
Size: 53 83 186 936 1215 1201 2468
Fragments arranged by size:
2468 1215 1201 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstXI BstZ17I DraIII EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI