(Linear) (Six-base) MAPSORT of: KIEE3211.seq Check: 6167 from: 1 to: 1437 KIEE3211.orf With 182 enzymes: SgfI * March 22, 2012 16:21 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1097 1437 Size: 1097 340 AatII G_ACGT'C Cuts at: 0 746 1437 Size: 746 691 Acc65I G'GTAC_C Cuts at: 0 1202 1437 Size: 1202 235 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 401 1295 1437 Size: 401 894 142 Fragments arranged by size: 894 401 142 AflIII A'CryG_T Cuts at: 0 430 477 1437 Size: 430 47 960 Fragments arranged by size: 960 430 47 AlwNI CAG_nnn'CTG Cuts at: 0 444 459 654 759 982 1017 1192 1437 Size: 444 15 195 105 223 35 175 245 Fragments arranged by size: 444 245 223 195 175 105 35 15 AseI AT'TA_AT Cuts at: 0 1412 1437 Size: 1412 25 AvaI C'yCGr_G Cuts at: 0 34 804 820 1437 Size: 34 770 16 617 Fragments arranged by size: 770 617 34 16 BamHI G'GATC_C Cuts at: 0 449 1437 Size: 449 988 BanI G'GyrC_C Cuts at: 0 615 1202 1437 Size: 615 587 235 Fragments arranged by size: 615 587 235 BanII G_rGCy'C Cuts at: 0 947 1437 Size: 947 490 BbsI GAAGACnn'nnnn_ Cuts at: 0 337 738 1377 1437 Size: 337 401 639 60 Fragments arranged by size: 639 401 337 60 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 795 829 1437 Size: 795 34 608 Fragments arranged by size: 795 608 34 BciVI GTATCCnnnnn_n' Cuts at: 0 37 1437 Size: 37 1400 BglI GCCn_nnn'nGGC Cuts at: 0 862 1437 Size: 862 575 BlpI GC'TnA_GC Cuts at: 0 1006 1437 Size: 1006 431 Bme1580I G_kGCm'C Cuts at: 0 173 1437 Size: 173 1264 BmgBI CAC'GTC Cuts at: 0 480 1437 Size: 480 957 BmrI ACTGGGnnnn_n' Cuts at: 0 39 1202 1437 Size: 39 1163 235 Fragments arranged by size: 1163 235 39 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 645 1437 Size: 645 792 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 112 283 482 572 658 679 1437 Size: 112 171 199 90 86 21 758 Fragments arranged by size: 758 199 171 112 90 86 21 BsaHI Gr'CG_yC Cuts at: 0 5 743 1061 1437 Size: 5 738 318 376 Fragments arranged by size: 738 376 318 5 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1321 1351 1437 Size: 1321 30 86 Fragments arranged by size: 1321 86 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 70 244 982 1437 Size: 70 174 738 455 Fragments arranged by size: 738 455 174 70 BseYI C'CCAG_C Cuts at: 0 340 1046 1437 Size: 340 706 391 Fragments arranged by size: 706 391 340 BsiHKAI G_wGCw'C Cuts at: 0 498 947 1437 Size: 498 449 490 Fragments arranged by size: 498 490 449 BsmBI CGTCTCn'nnnn_ Cuts at: 0 421 613 634 1437 Size: 421 192 21 803 Fragments arranged by size: 803 421 192 21 Bsp1286I G_dGCh'C Cuts at: 0 173 498 947 1437 Size: 173 325 449 490 Fragments arranged by size: 490 449 325 173 BspHI T'CATG_A Cuts at: 0 134 995 1437 Size: 134 861 442 Fragments arranged by size: 861 442 134 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1097 1272 1437 Size: 1097 175 165 Fragments arranged by size: 1097 175 165 BsrBI CCG'CTC Cuts at: 0 1161 1437 Size: 1161 276 BsrDI GCAATG_nn' Cuts at: 0 227 1437 Size: 227 1210 BsrGI T'GTAC_A Cuts at: 0 1214 1437 Size: 1214 223 BssHII G'CGCG_C Cuts at: 0 344 1437 Size: 344 1093 BstAPI GCAn_nnn'nTGC Cuts at: 0 87 1437 Size: 87 1350 BstBI TT'CG_AA Cuts at: 0 1388 1437 Size: 1388 49 BstYI r'GATC_y Cuts at: 0 120 449 1238 1437 Size: 120 329 789 199 Fragments arranged by size: 789 329 199 120 Bsu36I CC'TnA_GG Cuts at: 0 201 1437 Size: 201 1236 BtgI C'CryG_G Cuts at: 0 1066 1232 1437 Size: 1066 166 205 Fragments arranged by size: 1066 205 166 BtsI GCAGTG_nn' Cuts at: 0 840 1437 Size: 840 597 EaeI y'GGCC_r Cuts at: 0 296 580 1346 1437 Size: 296 284 766 91 Fragments arranged by size: 766 296 284 91 EarI CTCTTCn'nnn_ Cuts at: 0 377 526 1271 1437 Size: 377 149 745 166 Fragments arranged by size: 745 377 166 149 EcoICRI GAG'CTC Cuts at: 0 945 1437 Size: 945 492 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 112 283 401 482 572 658 679 1295 Size: 112 171 118 81 90 86 21 616 Cuts at: 1295 1437 Size: 142 Fragments arranged by size: 616 171 142 118 112 90 86 81 21 EcoNI CCTnn'n_nnAGG Cuts at: 0 997 1078 1437 Size: 997 81 359 Fragments arranged by size: 997 359 81 EcoO109I rG'GnC_Cy Cuts at: 0 1071 1371 1437 Size: 1071 300 66 Fragments arranged by size: 1071 300 66 HaeII r_GCGC'y Cuts at: 0 1017 1437 Size: 1017 420 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1230 1262 1398 1430 1437 Size: 1230 32 136 32 7 Fragments arranged by size: 1230 136 32 32 7 KpnI G_GTAC'C Cuts at: 0 1206 1437 Size: 1206 231 MluI A'CGCG_T Cuts at: 0 430 1437 Size: 430 1007 MscI TGG'CCA Cuts at: 0 582 1437 Size: 582 855 MslI CAynn'nnrTG Cuts at: 0 1000 1437 Size: 1000 437 MspA1I CmG'CkG Cuts at: 0 360 1068 1321 1437 Size: 360 708 253 116 Fragments arranged by size: 708 360 253 116 NcoI C'CATG_G Cuts at: 0 1232 1437 Size: 1232 205 NspI r_CATG'y Cuts at: 0 90 1437 Size: 90 1347 PasI CC'CwG_GG Cuts at: 0 459 1437 Size: 459 978 PflMI CCAn_nnn'nTGG Cuts at: 0 459 1017 1192 1437 Size: 459 558 175 245 Fragments arranged by size: 558 459 245 175 PpuMI rG'GwC_Cy Cuts at: 0 1071 1371 1437 Size: 1071 300 66 Fragments arranged by size: 1071 300 66 PshAI GACnn'nnGTC Cuts at: 0 766 1437 Size: 766 671 PstI C_TGCA'G Cuts at: 0 851 1259 1437 Size: 851 408 178 Fragments arranged by size: 851 408 178 PvuII CAG'CTG Cuts at: 0 360 1321 1437 Size: 360 961 116 Fragments arranged by size: 961 360 116 SacI G_AGCT'C Cuts at: 0 947 1437 Size: 947 490 SacII CC_GC'GG Cuts at: 0 1069 1437 Size: 1069 368 SapI GCTCTTCn'nnn_ Cuts at: 0 1271 1437 Size: 1271 166 SfcI C'TryA_G Cuts at: 0 847 1255 1308 1437 Size: 847 408 53 129 Fragments arranged by size: 847 408 129 53 SspI AAT'ATT Cuts at: 0 185 1437 Size: 185 1252 StyI C'CwwG_G Cuts at: 0 1232 1437 Size: 1232 205 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 486 1038 1437 Size: 486 552 399 Fragments arranged by size: 552 486 399 TatI w'GTAC_w Cuts at: 0 1214 1437 Size: 1214 223 Tth111I GACn'n_nGTC Cuts at: 0 505 1437 Size: 505 932 ZraI GAC'GTC Cuts at: 0 744 1437 Size: 744 693 Enzymes that do cut: AarI AatII Acc65I AcuI AflIII AlwNI AseI AvaI BamHI BanI BanII BbsI BcgI BciVI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI BsaHI BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstYI Bsu36I BtgI BtsI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KpnI MluI MscI MslI MspA1I NcoI NspI PasI PflMI PpuMI PshAI PstI PvuII SacI SacII SapI SfcI SspI StyI TaqII TatI Tth111I ZraI Enzymes that do not cut: AccI AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AsiSI AvrII BaeI BbeI BbvCI BclI BfrBI BglII BmtI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsgI BsiEI BsiWI BsmI BspEI BsrFI BssSI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI MfeI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PfoI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI SwaI TstI XbaI XcmI XhoI XmaI XmnI