Restriction map for
pFN21AE3108
##################################
# NO CUT ENZYMES ( 145 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BciVI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaWI BseAI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BsrDI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MseI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 487 GACGTC GACGT^C
AcyI 487 GACGTC G[GA]^CG[CT]C
AflIII 449 ACATGT A^C[GA][CT]GT
AluI 739 AGCT AG^CT
AlwI 441 GATCC GGATC(4/5)
AlwNI 544 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
BanII 30 GAGCCC G[GA]GC[CT]^C
BbsI 19 GAAGAC GAAGAC(2/6)
BclI 623 TGATCA T^GATCA
BmyI 30 GAGCCC G[AGT]GC[ACT]^C
BpmI 538 CTGGAG CTGGAG(16/14)
BpuAI 19 GAAGAC GAAGAC(2/6)
BsaHI 487 GACGTC G[GA]^CG[CT]C
BsaI 373 GAGACC GGTCTC(1/5)
BsgI 670 GTGCAG GTGCAG(16/14)
Bsp1286I 30 GAGCCC G[AGT]GC[ACT]^C
BspLU11I 449 ACATGT A^CATGT
BspMI 458 ACCTGC ACCTGC(4/8)
BsrBI 84 GAGCGG CCGCTC(-3/-3)
BsrFI 576 GCCGGC [GA]^CCGG[CT]
BstEII 365 GGTTACC G^GT[ACGT]ACC
BstPI 365 GGTTACC G^GT[ACGT]ACC
BstYI 440 AGATCC [GA]^GATC[CT]
Cfr10I 576 GCCGGC [GA]^CCGG[CT]
DrdI 481 GACTCCGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 377 CCACGG C^C[GA][CT]GG
EcoNI 183 CCTGAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 365 GGTTACC G^GT[ACGT]ACC
EcoT14I 230 CCAAGG C^C[AT][AT]GG
FbaI 623 TGATCA T^GATCA
HgaI 552 GACGC GACGC(5/10)
Hin1I 487 GACGTC G[GA]^CG[CT]C
HincII 108 GTCGAC GT[CT]^[GA]AC
HindII 108 GTCGAC GT[CT]^[GA]AC
MflI 440 AGATCC [GA]^GATC[CT]
NaeI 576 GCCGGC GCC^GGC
NgoMIV 576 GCCGGC G^CCGGC
NspI 449 ACATGT [GA]CATG^[CT]
SalI 108 GTCGAC G^TCGAC
SfcI 429 CTACAG C^T[GA][CT]AG
SmlI 360 CTCAAG C^T[CT][GA]AG
StuI 233 AGGCCT AGG^CCT
StyI 230 CCAAGG C^C[AT][AT]GG
TfiI 546 GATTC G^A[AT]TC
Tsp509I 253 AATT ^AATT
TspRI 93 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 440 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 108 GTCGAC GT^[AC][GT]AC
385 GTCTAC
AccII 36 CGCG CG^CG
632 CGCG
AfaI 249 GTAC GT^AC
558 GTAC
BanI 333 GGTGCC G^G[CT][GA]CC
713 GGCACC
BcnI 444 CCGGG CC^[GC]GG
513 CCGGG
BsiEI 48 CGACCG CG[GA][CT]^CG
110 CGACCG
BsmAI 373 GAGAC GTCTC(1/5)
498 GTCTC
BstUI 36 CGCG CG^CG
632 CGCG
DdeI 184 CTGAG C^T[ACGT]AG
758 CTGAG
DraII 206 AGGTCCT [GA]G^G[ACGT]CC[CT]
533 GGGACCT
EarI 466 CTCTTC CTCTTC(1/4)
503 GAAGAG
EcoO109I 206 AGGTCCT [GA]G^G[ACGT]CC[CT]
533 GGGACCT
HphI 81 GGTGA GGTGA(8/7)
697 GGTGA
Ksp632I 466 CTCTTC CTCTTC(1/4)
503 GAAGAG
MvnI 36 CGCG CG^CG
632 CGCG
NciI 444 CCGGG CC^[GC]GG
513 CCGGG
NlaIII 129 CATG CATG^
450 CATG
PpuMI 206 AGGTCCT [GA]G^G[AT]CC[CT]
533 GGGACCT
RsaI 249 GTAC GT^AC
558 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AvaII BbvI BsaJI BseRI
BsiYI BslI BsmFI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DpnII
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MwoI NdeII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI TaqI TseI
Tsp45I TthHB8I