Restriction map for
pFN21AE3108
(Circular) (Six-base) MAPSORT of: pFN21AE3108.seq Check: 8209 from: 1 to: 5536
pFN21AE3108 5536 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2472 2472
Size: 5536
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2496 3346 278
Size: 53 83 186 936 960 850 2468
Fragments arranged by size:
2468 960 936 850 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2114 2391 2879 1937
Size: 177 277 488 4594
Fragments arranged by size:
4594 488 277 177
Acc65I G'GTAC_C
Cuts at: 2857 2857
Size: 5536
AclI AA'CG_TT
Cuts at: 1563 3663 4036 1563
Size: 2100 373 3063
Fragments arranged by size:
3063 2100 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2327 3599 4983 5251 1632
Size: 695 1272 1384 268 1917
Fragments arranged by size:
1917 1384 1272 695 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5339
AflIII A'CryG_T
Cuts at: 1203 2454 4435 5412 1203
Size: 1251 1981 977 1327
Fragments arranged by size:
1981 1327 1251 977
AgeI A'CCGG_T
Cuts at: 4367 4367
Size: 5536
AhdI GACnn_n'nnGTC
Cuts at: 4265 4265
Size: 5536
AleI CACnn'nnGTG
Cuts at: 1166 2573 1166
Size: 1407 4129
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5536
AlwNI CAG_nnn'CTG
Cuts at: 2554 3279 4851 2554
Size: 725 1572 3239
Fragments arranged by size:
3239 1572 725
ApaLI G'TGCA_C
Cuts at: 3593 4749 3593
Size: 1156 4380
ApoI r'AATT_y
Cuts at: 1493 2845 3071 3223 5332 1493
Size: 1352 226 152 2109 1697
Fragments arranged by size:
2109 1697 1352 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4090 160
Size: 872 3058 1606
Fragments arranged by size:
3058 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5536
AvaI C'yCGr_G
Cuts at: 1870 1943 2861 1870
Size: 73 918 4545
Fragments arranged by size:
4545 918 73
BamHI G'GATC_C
Cuts at: 2866 3233 2866
Size: 367 5169
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2338 2718 2857 4312 618
Size: 325 217 627 551 380 139 1455 1842
Fragments arranged by size:
1842 1455 627 551 380 325 217 139
BanII G_rGCy'C
Cuts at: 1391 2039 2855 1391
Size: 648 816 4072
Fragments arranged by size:
4072 816 648
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2031 928
Size: 971 132 4433
Fragments arranged by size:
4433 971 132
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3727 3761 1903
Size: 34 1790 34 3678
Fragments arranged by size:
3678 1790 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3428 4638 3428
Size: 1210 4326
BclI T'GATC_A
Cuts at: 1548 2628 1548
Size: 1080 4456
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4147 136
Size: 107 122 71 631 605 2475 1525
Fragments arranged by size:
2475 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5531 5531
Size: 5536
BlpI GC'TnA_GC
Cuts at: 2953 2953
Size: 5536
Bme1580I G_kGCm'C
Cuts at: 1790 3597 4753 1790
Size: 1807 1156 2573
Fragments arranged by size:
2573 1807 1156
BmgBI CAC'GTC
Cuts at: 1318 2404 2524 1318
Size: 1086 120 4330
Fragments arranged by size:
4330 1086 120
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3267 4225 442
Size: 416 53 83 399 24 1850 958 1753
Fragments arranged by size:
1850 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5536
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2564 4196 1239
Size: 1325 1632 2579
Fragments arranged by size:
2579 1632 1325
Bpu10I CC'TnA_GC
Cuts at: 2189 2189
Size: 5536
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2350 3661 4526 4824 2350
Size: 1311 865 298 3062
Fragments arranged by size:
3062 1311 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2372 4199 882
Size: 617 873 1827 2219
Fragments arranged by size:
2219 1827 873 617
BsaAI yAC'GTr
Cuts at: 493 1204 3327 493
Size: 711 2123 2702
Fragments arranged by size:
2702 2123 711
BsaBI GATnn'nnATC
Cuts at: 5530 5530
Size: 5536
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2493 3343 3725 4360
Size: 53 83 186 936 960 850 382 635
Cuts at: 4360 275
Size: 1451
Fragments arranged by size:
1451 960 936 850 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3969 4367 4641 4788 5199 3969
Size: 398 274 147 411 4306
Fragments arranged by size:
4306 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2471 2501 1178
Size: 30 165 30 1068 30 4213
Fragments arranged by size:
4213 1068 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2606 2655 2739 1186
Size: 1420 49 84 3983
Fragments arranged by size:
3983 1420 84 49
BseYI C'CCAG_C
Cuts at: 2143 4739 5235 2143
Size: 2596 496 2444
Fragments arranged by size:
2596 2444 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2696 1163
Size: 573 960 4003
Fragments arranged by size:
4003 960 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2056 2118 2986 3747 4775 1540
Size: 275 186 55 62 868 761 1028 2301
Fragments arranged by size:
2301 1028 868 761 275 186 62 55
BsiHKAI G_wGCw'C
Cuts at: 3597 3682 4753 3597
Size: 85 1071 4380
Fragments arranged by size:
4380 1071 85
BsmI GAATG_Cn'
Cuts at: 3047 3140 3047
Size: 93 5443
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2039 2855 3597 3682 4753 1391
Size: 399 249 816 742 85 1071 2174
Fragments arranged by size:
2174 1071 816 742 399 249 85
BspEI T'CCGG_A
Cuts at: 5199 5199
Size: 5536
BspHI T'CATG_A
Cuts at: 3425 5155 3425
Size: 1730 3806
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2472 2891 844
Size: 1628 419 3489
Fragments arranged by size:
3489 1628 419
BsrBI CCG'CTC
Cuts at: 1394 1823 2091 3423 1394
Size: 429 268 1332 3507
Fragments arranged by size:
3507 1332 429 268
BsrDI GCAATG_nn'
Cuts at: 65 1248 4031 4205 65
Size: 1183 2783 174 1396
Fragments arranged by size:
2783 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2581 4180 4367 1700
Size: 110 771 1599 187 2869
Fragments arranged by size:
2869 1599 771 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5536
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5536
BssSI C'ACGA_G
Cuts at: 3596 4608 3596
Size: 1012 4524
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5536
BstBI TT'CG_AA
Cuts at: 4340 4340
Size: 5536
BstEII G'GTnAC_C
Cuts at: 1184 2370 1184
Size: 1186 4350
BstYI r'GATC_y
Cuts at: 1972 2445 2866 3233 3618 3635 5087 5173 5185
Size: 473 421 367 385 17 1452 86 12
Cuts at: 5185 5531 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 473 421 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2382 513
Size: 552 939 378 3667
Fragments arranged by size:
3667 939 552 378
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4797
Fragments arranged by size:
4797 683 56
BtsI GCAGTG_nn'
Cuts at: 2097 3052 3846 3866 2097
Size: 955 794 20 3767
Fragments arranged by size:
3767 955 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5536
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5501
DraI TTT'AAA
Cuts at: 2840 2840
Size: 5536
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2492 4543 809
Size: 507 1176 2051 1802
Fragments arranged by size:
2051 1802 1176 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2983 3872 5501
Size: 54 1425 50 275 18 1153 889 1629
Cuts at: 5501 8
Size: 43
Fragments arranged by size:
1629 1425 1153 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2983 1537
Size: 275 1171 4090
Fragments arranged by size:
4090 1171 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2477 2503 3466 1353
Size: 1124 26 963 3423
Fragments arranged by size:
3423 1124 963 26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2755 4123 4497 4643 387
Size: 1064 138 1166 1368 374 146 1280
Fragments arranged by size:
1368 1280 1166 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2327 2564 3599 4196 4983 5251 1239
Size: 393 695 237 1035 597 787 268 1524
Fragments arranged by size:
1524 1035 787 695 597 393 268 237
EcoNI CCTnn'n_nnAGG
Cuts at: 2192 2192
Size: 5536
EcoO109I rG'GnC_Cy
Cuts at: 2212 2539 2212
Size: 327 5209
EcoRI G'AATT_C
Cuts at: 2845 3223 2845
Size: 378 5158
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2602 2773 2602
Size: 171 5365
FspI TGC'GCA
Cuts at: 4042 4042
Size: 5536
HaeII r_GCGC'y
Cuts at: 1678 4683 1678
Size: 3005 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2471 2503 4177 4209 4251
Size: 32 135 32 899 32 1674 32 42
Cuts at: 4251 4283 1373
Size: 32 2626
Fragments arranged by size:
2626 1674 899 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2115 2880 3126 669
Size: 988 281 177 765 246 3079
Fragments arranged by size:
3079 988 765 281 246 177
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5536
HpaI GTT'AAC
Cuts at: 3126 3126
Size: 5536
KpnI G_GTAC'C
Cuts at: 2861 2861
Size: 5536
MfeI C'AATT_G
Cuts at: 3135 3135
Size: 5536
MluI A'CGCG_T
Cuts at: 5412 5412
Size: 5536
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2514 4650 4834 1305
Size: 1209 2136 184 2007
Fragments arranged by size:
2136 2007 1209 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3714
Fragments arranged by size:
3714 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2271 2426 2573 3494 3853 4012 518
Size: 648 1105 155 147 921 359 159 2042
Fragments arranged by size:
2042 1105 921 648 359 159 155 147
MspA1I CmG'CkG
Cuts at: 1606 2068 2106 2167 2173 2952 3629 4777 5022
Size: 462 38 61 6 779 677 1148 245
Cuts at: 5022 1606
Size: 2120
Fragments arranged by size:
2120 1148 779 677 462 245 61 38
6
NaeI GCC'GGC
Cuts at: 2583 2583
Size: 5536
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4045
Fragments arranged by size:
4045 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5536
NgoMIV G'CCGG_C
Cuts at: 2581 2581
Size: 5536
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5536
NotI GC'GGCC_GC
Cuts at: 2983 2983
Size: 5536
NspI r_CATG'y
Cuts at: 1132 1229 2458 2894 4439 1132
Size: 97 1229 436 1545 2229
Fragments arranged by size:
2229 1545 1229 436 97
PasI CC'CwG_GG
Cuts at: 1348 2780 1348
Size: 1432 4104
PciI A'CATG_T
Cuts at: 2454 4435 2454
Size: 1981 3555
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5536
PfoI T'CCnGG_A
Cuts at: 2448 2517 2685 2448
Size: 69 168 5299
Fragments arranged by size:
5299 168 69
PmeI GTTT'AAAC
Cuts at: 2840 2840
Size: 5536
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3605 3637 5118 5150 3605
Size: 32 1481 32 3991
Fragments arranged by size:
3991 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2212 2539 2212
Size: 327 5209
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5536
PsiI TTA'TAA
Cuts at: 3106 3106
Size: 5536
PstI C_TGCA'G
Cuts at: 830 2888 830
Size: 2058 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5536
SalI G'TCGA_C
Cuts at: 1936 2113 2878 1936
Size: 177 765 4594
Fragments arranged by size:
4594 765 177
SbfI CC_TGCA'GG
Cuts at: 2888 2888
Size: 5536
ScaI AGT'ACT
Cuts at: 3784 5496 3784
Size: 1712 3824
SfcI C'TryA_G
Cuts at: 826 1045 2434 2884 4019 4700 4891 826
Size: 219 1389 450 1135 681 191 1471
Fragments arranged by size:
1471 1389 1135 681 450 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5536
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5536
SmaI CCC'GGG
Cuts at: 1872 2863 1872
Size: 991 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2365 3640 4541 4803 820
Size: 197 926 422 1275 901 262 1553
Fragments arranged by size:
1553 1275 926 901 422 262 197
SnaBI TAC'GTA
Cuts at: 493 3327 493
Size: 2834 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5536
SphI G_CATG'C
Cuts at: 1132 2894 1132
Size: 1762 3774
SspI AAT'ATT
Cuts at: 5 52 3460 5
Size: 47 3408 2081
Fragments arranged by size:
3408 2081 47
StuI AGG'CCT
Cuts at: 1517 2240 1517
Size: 723 4813
StyI C'CwwG_G
Cuts at: 513 1065 2004 2235 2975 513
Size: 552 939 231 740 3074
Fragments arranged by size:
3074 939 740 552 231
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3563 3580 3733 3918 4363 325
Size: 1255 1983 17 153 185 445 1498
Fragments arranged by size:
1983 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2253 2562 3782
Size: 274 80 33 51 1443 276 309 1220
Cuts at: 3782 5494 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1220 309 276 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5496 5528 5496
Size: 32 5504
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5536
XbaI T'CTAG_A
Cuts at: 2872 2872
Size: 5536
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5536
XmaI C'CCGG_G
Cuts at: 1870 2861 1870
Size: 991 4545
XmnI GAAnn'nnTTC
Cuts at: 3665 3665
Size: 5536
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2494 3344 276
Size: 53 83 186 936 960 850 2468
Fragments arranged by size:
2468 960 936 850 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI