################################## # NO CUT ENZYMES ( 170 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BbrPI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HincII HindII HindIII HpaI HphI KpnI KspI MaeI MaeII MaeIII MamI MfeI MflI MluI MroI MseI MslI MunI MvnI NaeI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacII SalI SapI SbfI ScaI SexAI SfaNI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI SwaI TaqI Tru9I Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AlwNI 193 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 136 GGGCCC GGGCC^C AspHI 352 GAGCTC G[AT]GC[AT]^C BalI 128 TGGCCA TGG^CCA BanI 46 GGCGCC G^G[CT][GA]CC BbeI 46 GGCGCC GGCGC^C BbsI 318 GAAGAC GAAGAC(2/6) BbvCI 346 GCTGAGG CCTCAGC(-5/-2) BglI 121 GCCGACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 107 CTGGAG CTGGAG(16/14) BpuAI 318 GAAGAC GAAGAC(2/6) BseRI 349 GAGGAG GAGGAG(10/8) BsiEI 399 CGGTCG CG[GA][CT]^CG BsiHKAI 352 GAGCTC G[AT]GC[AT]^C BsrFI 292 ACCGGC [GA]^CCGG[CT] BsrI 63 ACTGG ACTGG(1/-1) CfoI 47 GCGC GCG^C Cfr10I 292 ACCGGC [GA]^CCGG[CT] DpnII 21 GATC ^GATC DraIII 148 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 128 TGGCCA [CT]^GGCC[GA] EcoNI 342 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 310 GCGGG CCCGC(4/6) HaeII 46 GGCGCC [GA]GCGC^[CT] HapII 293 CCGG C^CGG HgaI 85 GACGC GACGC(5/10) HhaI 47 GCGC GCG^C HinP1I 47 GCGC G^CGC HinfI 254 GATTC G^A[ACGT]TC HpaII 293 CCGG C^CGG KasI 46 GGCGCC G^GCGCC MboI 21 GATC ^GATC MluNI 128 TGGCCA TGG^CCA MscI 128 TGGCCA TGG^CCA MspI 293 CCGG C^CGG NarI 46 GGCGCC GG^CGCC NdeII 21 GATC ^GATC NspI 240 GCATGC [GA]CATG^[CT] SacI 352 GAGCTC GAGCT^C Sau3AI 21 GATC ^GATC SfiI 120 GGCCGACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 46 GGCGCC GGC^GCC SphI 240 GCATGC GCATG^C StuI 7 AGGCCT AGG^CCT TfiI 254 GATTC G^A[AT]TC TspRI 282 CAGTG CA[GC]TG[ACGT][ACGT]^ XcmI 80 CCACAGACGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AcyI 46 GGCGCC G[GA]^CG[CT]C 85 GACGCC BsaHI 46 GGCGCC G[GA]^CG[CT]C 85 GACGCC DdeI 175 CTGAG C^T[ACGT]AG 347 CTGAG DsaI 89 CCATGG C^C[GA][CT]GG 131 CCATGG EarI 31 CTCTTC CTCTTC(1/4) 71 GAAGAG EcoT14I 89 CCATGG C^C[AT][AT]GG 131 CCATGG Hin1I 46 GGCGCC G[GA]^CG[CT]C 85 GACGCC Ksp632I 31 CTCTTC CTCTTC(1/4) 71 GAAGAG MspA1I 40 CCGCTG C[AC]G^C[GT]G 324 CAGCGG NcoI 89 CCATGG C^CATGG 131 CCATGG PstI 220 CTGCAG CTGCA^G 249 CTGCAG StyI 89 CCATGG C^C[AT][AT]GG 131 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AciI AluI BanII BbvI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI Cac8I Cfr13I DraII EcoO109I EcoRII Fnu4HI HaeIII ItaI MboII MnlI MvaI MwoI NlaIII NlaIV Sau96I ScrFI SfcI TseI