################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BalI BbsI BciVI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BseAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I HincII HindII HindIII HpaI KpnI MamI MfeI MluI MluNI MroI MscI MseI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SexAI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp45I Tsp509I TspRI Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AcyI 54 GGCGCC G[GA]^CG[CT]C Alw44I 497 GTGCAC G^TGCAC ApaI 342 GGGCCC GGGCC^C ApaLI 497 GTGCAC G^TGCAC BamHI 632 GGATCC G^GATCC BbeI 54 GGCGCC GGCGC^C BbvCI 22 GCTGAGG CCTCAGC(-5/-2) BcgI 125 GCAACGTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 263 TGATCA T^GATCA BfaI 652 CTAG C^TAG BpmI 523 CTGGAG CTGGAG(16/14) BsaHI 54 GGCGCC G[GA]^CG[CT]C BsaWI 470 ACCGGA [AT]^CCGG[AT] BseRI 655 GAGGAG GAGGAG(10/8) BsmFI 60 GGGAC GGGAC(10/14) BspDI 448 ATCGAT AT^CGAT BstYI 632 GGATCC [GA]^GATC[CT] ClaI 448 ATCGAT AT^CGAT EcoT14I 1091 CCATGG C^C[AT][AT]GG FbaI 263 TGATCA T^GATCA FseI 933 GGCCGGCC GGCCGG^CC HgaI 734 GCGTC GACGC(5/10) Hin1I 54 GGCGCC G[GA]^CG[CT]C HinfI 700 GACTC G^A[ACGT]TC KasI 54 GGCGCC G^GCGCC MaeI 652 CTAG C^TAG MaeIII 503 GTAAC ^GT[ACGT]AC MflI 632 GGATCC [GA]^GATC[CT] MslI 564 CATTGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 54 GGCGCC GG^CGCC NcoI 1091 CCATGG C^CATGG PleI 700 GACTC GAGTC(4/5) PstI 988 CTGCAG CTGCA^G PvuII 928 CAGCTG CAG^CTG SacI 276 GAGCTC GAGCT^C SfcI 988 CTGCAG C^T[GA][CT]AG SfiI 712 GGCCCCGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 54 GGCGCC GGC^GCC SmaI 646 CCCGGG CCC^GGG SmlI 37 CTCAAG C^T[CT][GA]AG SphI 725 GCATGC GCATG^C StyI 1091 CCATGG C^C[AT][AT]GG XhoII 632 GGATCC [GA]^GATC[CT] XmaCI 646 CCCGGG C^CCGGG XmaI 646 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AfaI 111 GTAC GT^AC 603 GTAC AvaI 133 CTCGGG C^[CT]CG[GA]G 646 CCCGGG AviII 785 TGCGCA TGC^GCA 857 TGCGCA BbrPI 376 CACGTG CAC^GTG 865 CACGTG BglI 713 GCCCCGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 743 GCCACCGCGGC BsoBI 133 CTCGGG C^[CT]CG[GA]G 646 CCCGGG BspMI 770 ACCTGC ACCTGC(4/8) 1082 ACCTGC BsrI 63 ACTGG ACTGG(1/-1) 843 CCAGT BssHII 942 GCGCGC G^CGCGC 959 GCGCGC BstAPI 725 GCATGCGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1077 GCACCACCTGC DdeI 23 CTGAG C^T[ACGT]AG 272 CTCAG EarI 145 CTCTTC CTCTTC(1/4) 954 GAAGAG FspI 785 TGCGCA TGC^GCA 857 TGCGCA Ksp632I 145 CTCTTC CTCTTC(1/4) 954 GAAGAG KspI 716 CCGCGG CCGC^GG 747 CCGCGG NaeI 934 GCCGGC GCC^GGC 1086 GCCGGC NgoMIV 934 GCCGGC G^CCGGC 1086 GCCGGC NspI 725 GCATGC [GA]CATG^[CT] 782 ACATGC PflMI 311 CCAAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 792 CCACACGCTGG PmaCI 376 CACGTG CAC^GTG 865 CACGTG PmlI 376 CACGTG CAC^GTG 865 CACGTG RsaI 111 GTAC GT^AC 603 GTAC SacII 716 CCGCGG CCGC^GG 747 CCGCGG SapI 954 GAAGAGC GCTCTTC(1/4) 144 GCTCTTC TaqI 149 TCGA T^CGA 449 TCGA TthHB8I 149 TCGA T^CGA 449 TCGA Van91I 311 CCAAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 792 CCACACGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AccII AciI AluI AlwI AlwNI AspHI AvaII BanI BanII BbvI BcnI BmyI BsaAI BsaJI BsgI BsiEI BsiHKAI BsiYI BslI Bsp1286I BsrFI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TseI