(Circular) (Six-base) MAPSORT of: pFN21AB9711.seq Check: 736 from: 1 to: 5806 pFN21AB9711 5806 bp With 182 enzymes: SgfI * January 30, 2014 17:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3096 3096 Size: 5806 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3616 278 Size: 53 83 186 936 2080 2468 Fragments arranged by size: 2468 2080 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3149 1937 Size: 1212 4594 Acc65I G'GTAC_C Cuts at: 3127 3127 Size: 5806 AclI AA'CG_TT Cuts at: 1563 3933 4306 1563 Size: 2370 373 3063 Fragments arranged by size: 3063 2370 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2075 2827 3869 5253 5521 1632 Size: 443 752 1042 1384 268 1917 Fragments arranged by size: 1917 1384 1042 752 443 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5609 AflIII A'CryG_T Cuts at: 1203 4705 5682 1203 Size: 3502 977 1327 Fragments arranged by size: 3502 1327 977 AgeI A'CCGG_T Cuts at: 4637 4637 Size: 5806 AhdI GACnn_n'nnGTC Cuts at: 4535 4535 Size: 5806 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5806 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2792 2888 3008 1184 Size: 1608 96 120 3982 Fragments arranged by size: 3982 1608 120 96 AlwNI CAG_nnn'CTG Cuts at: 2239 2527 2596 3549 5121 2239 Size: 288 69 953 1572 2924 Fragments arranged by size: 2924 1572 953 288 69 ApaI G_GGCC'C Cuts at: 2351 2351 Size: 5806 ApaLI G'TGCA_C Cuts at: 2502 3863 5019 2502 Size: 1361 1156 3289 Fragments arranged by size: 3289 1361 1156 ApoI r'AATT_y Cuts at: 1493 3115 3341 3493 5602 1493 Size: 1622 226 152 2109 1697 Fragments arranged by size: 2109 1697 1622 226 152 AseI AT'TA_AT Cuts at: 160 1032 4360 160 Size: 872 3328 1606 Fragments arranged by size: 3328 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5806 AvaI C'yCGr_G Cuts at: 1870 1943 2138 2651 3131 1870 Size: 73 195 513 480 4545 Fragments arranged by size: 4545 513 480 195 73 BamHI G'GATC_C Cuts at: 2637 3136 3503 2637 Size: 499 367 4940 Fragments arranged by size: 4940 499 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2059 2546 2970 3127 4582 Size: 325 217 627 272 487 424 157 1455 Cuts at: 4582 618 Size: 1842 Fragments arranged by size: 1842 1455 627 487 424 325 272 217 157 BanII G_rGCy'C Cuts at: 1391 2015 2285 2351 3125 1391 Size: 624 270 66 774 4072 Fragments arranged by size: 4072 774 624 270 66 BbeI G_GCGC'C Cuts at: 2063 2063 Size: 5806 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4835 BbvCI CC'TCA_GC Cuts at: 2028 2028 Size: 5806 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2119 2153 3997 4031 1903 Size: 34 182 34 1844 34 3678 Fragments arranged by size: 3678 1844 182 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3698 4908 3698 Size: 1210 4596 BclI T'GATC_A Cuts at: 1548 2268 1548 Size: 720 5086 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2724 2754 4417 Size: 107 122 71 631 605 1052 30 1663 Cuts at: 4417 136 Size: 1525 Fragments arranged by size: 1663 1525 1052 631 605 122 107 71 30 BglII A'GATC_T Cuts at: 5801 5801 Size: 5806 BlpI GC'TnA_GC Cuts at: 3223 3223 Size: 5806 Bme1580I G_kGCm'C Cuts at: 1790 2351 2506 2652 3867 5023 1790 Size: 561 155 146 1215 1156 2573 Fragments arranged by size: 2573 1215 1156 561 155 146 BmgBI CAC'GTC Cuts at: 1318 2322 1318 Size: 1004 4802 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3537 4495 442 Size: 416 53 83 399 24 2120 958 1753 Fragments arranged by size: 2120 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5806 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2549 4466 1239 Size: 1310 1917 2579 Fragments arranged by size: 2579 1917 1310 Bpu10I CC'TnA_GC Cuts at: 2028 2028 Size: 5806 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2027 3931 4796 5094 2027 Size: 1904 865 298 2739 Fragments arranged by size: 2739 1904 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4469 882 Size: 617 2970 2219 Fragments arranged by size: 2970 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2383 2507 2872 3597 493 Size: 711 1179 124 365 725 2702 Fragments arranged by size: 2702 1179 725 711 365 124 BsaBI GATnn'nnATC Cuts at: 5800 5800 Size: 5806 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2060 3613 3995 4630 Size: 53 83 186 936 527 1553 382 635 Cuts at: 4630 275 Size: 1451 Fragments arranged by size: 1553 1451 936 635 527 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2475 4239 4637 4911 5058 5469 2475 Size: 1764 398 274 147 411 2812 Fragments arranged by size: 2812 1764 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5581 Fragments arranged by size: 5581 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2675 1186 Size: 1489 4317 BseYI C'CCAG_C Cuts at: 2981 5009 5505 2981 Size: 2028 496 3282 Fragments arranged by size: 3282 2028 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2087 2252 2864 1163 Size: 573 351 165 612 4105 Fragments arranged by size: 4105 612 573 351 165 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2122 2362 2978 3256 4017 5045 Size: 275 186 121 240 616 278 761 1028 Cuts at: 5045 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 616 278 275 240 186 121 BsiHKAI G_wGCw'C Cuts at: 2022 2043 2139 2285 2382 2506 3867 3952 5023 Size: 21 96 146 97 124 1361 85 1071 Cuts at: 5023 2022 Size: 2805 Fragments arranged by size: 2805 1361 1071 146 124 97 96 85 21 BsmI GAATG_Cn' Cuts at: 3317 3410 3317 Size: 93 5713 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2022 2043 2139 2285 2351 2382 Size: 399 225 7 21 96 146 66 31 Cuts at: 2382 2506 2652 3125 3867 3952 5023 1391 Size: 124 146 473 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 473 399 225 146 146 124 96 85 66 31 21 7 BspEI T'CCGG_A Cuts at: 5469 5469 Size: 5806 BspHI T'CATG_A Cuts at: 3695 5425 3695 Size: 1730 4076 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2784 3096 3161 844 Size: 1940 312 65 3489 Fragments arranged by size: 3489 1940 312 65 BsrBI CCG'CTC Cuts at: 1394 1823 3693 1394 Size: 429 1870 3507 Fragments arranged by size: 3507 1870 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4301 4475 65 Size: 1183 3053 174 1396 Fragments arranged by size: 3053 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2357 2939 2973 3091 4450 4637 1700 Size: 110 547 582 34 118 1359 187 2869 Fragments arranged by size: 2869 1359 582 547 187 118 110 34 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5806 BssHII G'CGCG_C Cuts at: 1925 2947 2964 1925 Size: 1022 17 4767 Fragments arranged by size: 4767 1022 17 BssSI C'ACGA_G Cuts at: 3866 4878 3866 Size: 1012 4794 BstAPI GCAn_nnn'nTGC Cuts at: 794 2736 3088 794 Size: 1942 352 3512 Fragments arranged by size: 3512 1942 352 BstBI TT'CG_AA Cuts at: 4610 4610 Size: 5806 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5806 BstXI CCAn_nnnn'nTGG Cuts at: 3103 3103 Size: 5806 BstYI r'GATC_y Cuts at: 1972 2637 3136 3503 3888 3905 5357 5443 5455 Size: 665 499 367 385 17 1452 86 12 Cuts at: 5455 5801 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 665 499 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2721 2752 3096 513 Size: 552 1656 31 344 3223 Fragments arranged by size: 3223 1656 552 344 31 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2257 2749 487 Size: 683 56 1031 492 3544 Fragments arranged by size: 3544 1031 683 492 56 BtsI GCAGTG_nn' Cuts at: 3322 4116 4136 3322 Size: 794 20 4992 Fragments arranged by size: 4992 794 20 ClaI AT'CG_AT Cuts at: 1554 2454 1554 Size: 900 4906 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5771 DraI TTT'AAA Cuts at: 3110 3110 Size: 5806 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4813 809 Size: 507 3497 1802 Fragments arranged by size: 3497 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2076 2119 2359 Size: 54 1425 50 275 18 246 43 240 Cuts at: 2359 2937 2975 3093 3253 4142 5771 8 Size: 578 38 118 160 889 1629 43 Fragments arranged by size: 1629 1425 889 578 275 246 240 160 118 54 50 43 43 38 18 EagI C'GGCC_G Cuts at: 1537 1812 2119 2359 2975 3253 1537 Size: 275 307 240 616 278 4090 Fragments arranged by size: 4090 616 307 278 275 240 EarI CTCTTCn'nnn_ Cuts at: 1353 2156 2954 3736 1353 Size: 803 798 782 3423 Fragments arranged by size: 3423 803 798 782 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2717 4393 4767 4913 387 Size: 1064 138 1128 1676 374 146 1280 Fragments arranged by size: 1676 1280 1128 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2283 2283 Size: 5806 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 2549 2827 3869 4466 5253 5521 Size: 393 443 474 278 1042 597 787 268 Cuts at: 5521 1239 Size: 1524 Fragments arranged by size: 1524 1042 787 597 474 443 393 278 268 EcoO109I rG'GnC_Cy Cuts at: 2032 2299 2348 2032 Size: 267 49 5490 Fragments arranged by size: 5490 267 49 EcoRI G'AATT_C Cuts at: 3115 3493 3115 Size: 378 5428 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2206 2206 Size: 5806 FseI GG_CCGG'CC Cuts at: 2943 2943 Size: 5806 FspI TGC'GCA Cuts at: 2792 2864 4312 2792 Size: 72 1448 4286 Fragments arranged by size: 4286 1448 72 FspAI rTGC'GCAy Cuts at: 2792 2792 Size: 5806 HaeII r_GCGC'y Cuts at: 1678 2063 2075 2896 2925 4953 1678 Size: 385 12 821 29 2028 2531 Fragments arranged by size: 2531 2028 821 385 29 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2260 2292 2727 2759 4447 Size: 32 135 32 688 32 435 32 1688 Cuts at: 4447 4479 4521 4553 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1688 688 435 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3150 3396 669 Size: 988 281 1212 246 3079 Fragments arranged by size: 3079 1212 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5806 HpaI GTT'AAC Cuts at: 3396 3396 Size: 5806 KasI G'GCGC_C Cuts at: 2059 2059 Size: 5806 KpnI G_GTAC'C Cuts at: 3131 3131 Size: 5806 MfeI C'AATT_G Cuts at: 3405 3405 Size: 5806 MluI A'CGCG_T Cuts at: 5682 5682 Size: 5806 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2118 4920 5104 1305 Size: 813 2802 184 2007 Fragments arranged by size: 2802 2007 813 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3984 Fragments arranged by size: 3984 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2573 3764 4123 4282 518 Size: 648 1407 1191 359 159 2042 Fragments arranged by size: 2042 1407 1191 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2124 2723 2754 2935 3222 3899 5047 5292 Size: 518 599 31 181 287 677 1148 245 Cuts at: 5292 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 599 518 287 245 181 31 NaeI GCC'GGC Cuts at: 2941 3093 2941 Size: 152 5654 NarI GG'CG_CC Cuts at: 2060 2060 Size: 5806 NcoI C'CATG_G Cuts at: 513 1065 3096 513 Size: 552 2031 3223 Fragments arranged by size: 3223 2031 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5806 NgoMIV G'CCGG_C Cuts at: 2939 3091 2939 Size: 152 5654 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5806 NotI GC'GGCC_GC Cuts at: 3253 3253 Size: 5806 NspI r_CATG'y Cuts at: 1132 1229 2734 2791 3164 4709 1132 Size: 97 1505 57 373 1545 2229 Fragments arranged by size: 2229 1545 1505 373 97 57 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5806 PciI A'CATG_T Cuts at: 4705 4705 Size: 5806 PflMI CCAn_nnn'nTGG Cuts at: 1108 2322 2803 1108 Size: 1214 481 4111 Fragments arranged by size: 4111 1214 481 PmeI GTTT'AAAC Cuts at: 3110 3110 Size: 5806 PmlI CAC'GTG Cuts at: 2383 2872 2383 Size: 489 5317 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3875 3907 5388 5420 3875 Size: 32 1481 32 4261 Fragments arranged by size: 4261 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5806 PsiI TTA'TAA Cuts at: 3376 3376 Size: 5806 PspOMI G'GGCC_C Cuts at: 2347 2347 Size: 5806 PstI C_TGCA'G Cuts at: 830 2997 3158 830 Size: 2167 161 3478 Fragments arranged by size: 3478 2167 161 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5806 PvuII CAG'CTG Cuts at: 2935 2935 Size: 5806 SacI G_AGCT'C Cuts at: 2285 2285 Size: 5806 SacII CC_GC'GG Cuts at: 2724 2755 2724 Size: 31 5775 SalI G'TCGA_C Cuts at: 1936 3148 1936 Size: 1212 4594 SapI GCTCTTCn'nnn_ Cuts at: 2156 2954 2156 Size: 798 5008 SbfI CC_TGCA'GG Cuts at: 3158 3158 Size: 5806 ScaI AGT'ACT Cuts at: 4054 5766 4054 Size: 1712 4094 SfcI C'TryA_G Cuts at: 826 1045 2993 3154 4289 4970 5161 826 Size: 219 1948 161 1135 681 191 1471 Fragments arranged by size: 1948 1471 1135 681 219 191 161 SfiI GGCCn_nnn'nGGCC Cuts at: 2724 2724 Size: 5806 SfoI GGC'GCC Cuts at: 2061 2061 Size: 5806 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5806 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5806 SmaI CCC'GGG Cuts at: 1872 2653 3133 1872 Size: 781 480 4545 Fragments arranged by size: 4545 781 480 SmlI C'TyrA_G Cuts at: 820 1017 1943 2042 3910 4811 5073 820 Size: 197 926 99 1868 901 262 1553 Fragments arranged by size: 1868 1553 926 901 262 197 99 SnaBI TAC'GTA Cuts at: 493 3597 493 Size: 3104 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5806 SphI G_CATG'C Cuts at: 1132 2734 3164 1132 Size: 1602 430 3774 Fragments arranged by size: 3774 1602 430 SrfI GCCC'GGGC Cuts at: 2653 2653 Size: 5806 SspI AAT'ATT Cuts at: 5 52 3730 5 Size: 47 3678 2081 Fragments arranged by size: 3678 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5806 StyI C'CwwG_G Cuts at: 513 1065 3096 3245 513 Size: 552 2031 149 3074 Fragments arranged by size: 3074 2031 552 149 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3833 3850 4003 4188 4633 325 Size: 1255 2253 17 153 185 445 1498 Fragments arranged by size: 2253 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4052 5764 96 Size: 274 80 33 51 1443 2075 1712 138 Fragments arranged by size: 2075 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2629 2661 5766 5798 2629 Size: 32 3105 32 2637 Fragments arranged by size: 3105 2637 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5806 XbaI T'CTAG_A Cuts at: 3142 3142 Size: 5806 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5806 XmaI C'CCGG_G Cuts at: 1870 2651 3131 1870 Size: 781 480 4545 Fragments arranged by size: 4545 781 480 XmnI GAAnn'nnTTC Cuts at: 3935 3935 Size: 5806 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3614 276 Size: 53 83 186 936 2080 2468 Fragments arranged by size: 2468 2080 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SanDI SexAI SwaI XcmI