################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaBI BsaHI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MroI MseI MspA1I MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI Tru9I Tsp509I TspRI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 262 GTCTAC GT^[AC][GT]AC AfaI 81 GTAC GT^AC AlwI 429 GATCC GGATC(4/5) AocI 204 CCTCAGG CC^T[ACGT]AGG ApaI 209 GGGCCC GGGCC^C AvaI 139 CCCGAG C^[CT]CG[GA]G AvaII 277 GGTCC G^G[AT]CC BalI 89 TGGCCA TGG^CCA BanI 366 GGCACC G^G[CT][GA]CC BanII 209 GGGCCC G[GA]GC[CT]^C BbsI 313 GTCTTC GAAGAC(2/6) BpuAI 313 GTCTTC GAAGAC(2/6) BsaAI 82 TACGTG [CT]AC^GT[GA] BsaI 229 GAGACC GGTCTC(1/5) BsaWI 215 ACCGGA [AT]^CCGG[AT] BsoBI 139 CCCGAG C^[CT]CG[GA]G BsrBI 375 CCGCTC CCGCTC(-3/-3) BsrI 168 CCAGT ACTGG(1/-1) BssSI 319 CTCGTG CACGAG(-5/-1) BstYI 428 AGATCC [GA]^GATC[CT] Bsu36I 204 CCTCAGG CC^T[ACGT]AGG CfoI 410 GCGC GCG^C DpnII 429 GATC ^GATC DraIII 337 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 89 TGGCCA [CT]^GGCC[GA] Eco81I 204 CCTCAGG CC^T[ACGT]AGG EcoT14I 416 CCATGG C^C[AT][AT]GG HhaI 410 GCGC GCG^C HinP1I 410 GCGC G^CGC HphI 18 GGTGA GGTGA(8/7) MaeIII 382 GTGAC ^GT[ACGT]AC MboI 429 GATC ^GATC MflI 428 AGATCC [GA]^GATC[CT] MluNI 89 TGGCCA TGG^CCA MscI 89 TGGCCA TGG^CCA MslI 72 CATCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 416 CCATGG C^CATGG NdeII 429 GATC ^GATC PflMI 9 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 276 GGGTCCT [GA]G^G[AT]CC[CT] RsaI 81 GTAC GT^AC Sau3AI 429 GATC ^GATC SfiI 33 GGCCCTCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC StuI 125 AGGCCT AGG^CCT StyI 416 CCATGG C^C[AT][AT]GG Tsp45I 382 GTGAC ^GT[GC]AC Van91I 9 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 428 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AluI 359 AGCT AG^CT 401 AGCT BcnI 30 CCCGG CC^[GC]GG 39 CCCGG BglI 34 GCCCTCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 412 GCCTCCATGGC BmyI 209 GGGCCC G[AGT]GC[ACT]^C 365 GGGCAC BsmAI 153 GAGAC GTCTC(1/5) 229 GAGAC Bsp1286I 209 GGGCCC G[AGT]GC[ACT]^C 365 GGGCAC DdeI 151 CTGAG C^T[ACGT]AG 205 CTCAG DsaI 341 CCGTGG C^C[GA][CT]GG 416 CCATGG FauI 446 CCCGC CCCGC(4/6) 363 GCGGG MaeII 83 ACGT A^CGT 191 ACGT MboII 120 GAAGA GAAGA(8/7) 314 TCTTC MwoI 34 GCCCTCCCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 412 GCCTCCATGGC NciI 30 CCCGG CC^[GC]GG 39 CCCGG NlaIII 225 CATG CATG^ 417 CATG PstI 54 CTGCAG CTGCA^G 424 CTGCAG SfaNI 145 GATGC GCATC(5/9) 300 GATGC SfcI 54 CTGCAG C^T[GA][CT]AG 424 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AciI BbvI BsaJI BsiYI BslI BssKI BstF5I BstNI Cac8I Cfr13I DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HpaII ItaI MnlI MspI MvaI NlaIV Sau96I ScrFI TseI