################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AccI AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AvrII BbeI BbrPI BbvCI BciVI BcnI BfaI BfrI BglII BlnI BsaAI BsaBI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstXI BstZ17I Bsu36I ClaI CpoI DrdI Eam1105I Eco47III Eco81I EcoNI EcoRI EcoT22I FseI HincII HindII HindIII HpaI KasI KspI MaeI MamI MfeI MluI MslI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp509I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 604 GACGTC GACGT^C Acc65I 1220 GGTACC G^GTACC AccII 245 CGCG CG^CG AlwNI 43 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp718I 1220 GGTACC G^GTACC AspHI 1234 GAGCAC G[AT]GC[AT]^C AspI 295 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 88 CCCGAG C^[CT]CG[GA]G AviII 968 TGCGCA TGC^GCA BanI 1220 GGTACC G^G[CT][GA]CC BanII 66 GGGCTC G[GA]GC[CT]^C BcgI 948 CGAGAGCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 701 TGATCA T^GATCA BglI 1203 GCCCGACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 229 GCTGAGC GC^T[ACGT]AGC Bpu1102I 229 GCTGAGC GC^T[ACGT]AGC BsaI 667 GAGACC GGTCTC(1/5) BsiEI 251 CGGCCG CG[GA][CT]^CG BsiHKAI 1234 GAGCAC G[AT]GC[AT]^C BsmBI 580 CGTCTC CGTCTC(1/5) BsoBI 88 CCCGAG C^[CT]CG[GA]G BspMI 643 ACCTGC ACCTGC(4/8) BsrBI 91 GAGCGG CCGCTC(-3/-3) BsrFI 110 ACCGGC [GA]^CCGG[CT] BstEII 999 GGTGACC G^GT[ACGT]ACC BstPI 999 GGTGACC G^GT[ACGT]ACC BstUI 245 CGCG CG^CG CelII 229 GCTGAGC GC^T[ACGT]AGC Cfr10I 110 ACCGGC [GA]^CCGG[CT] DraI 808 TTTAAA TTT^AAA DraIII 1045 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 251 CGGCCG C^GGCCG EarI 801 GAAGAG CTCTTC(1/4) EclXI 251 CGGCCG C^GGCCG Eco52I 251 CGGCCG C^GGCCG EcoO65I 999 GGTGACC G^GT[ACGT]ACC EcoRV 1039 GATATC GAT^ATC FbaI 701 TGATCA T^GATCA FspI 968 TGCGCA TGC^GCA KpnI 1220 GGTACC GGTAC^C Ksp632I 801 GAAGAG CTCTTC(1/4) MvnI 245 CGCG CG^CG PflFI 295 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 83 AGGACCC [GA]G^G[AT]CC[CT] PstI 460 CTGCAG CTGCA^G SapI 801 GAAGAGC GCTCTTC(1/4) SfcI 460 CTGCAG C^T[GA][CT]AG Tth111I 295 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccIII 20 TCCGGA T^CCGGA 847 TCCGGA AcyI 202 GGCGTC G[GA]^CG[CT]C 604 GACGTC AfaI 39 GTAC GT^AC 1221 GTAC BamHI 77 GGATCC G^GATCC 401 GGATCC BbsI 292 GAAGAC GAAGAC(2/6) 943 GAAGAC BpuAI 292 GAAGAC GAAGAC(2/6) 943 GAAGAC BsaHI 202 GGCGTC G[GA]^CG[CT]C 604 GACGTC BseAI 20 TCCGGA T^CCGGA 847 TCCGGA BspEI 20 TCCGGA T^CCGGA 847 TCCGGA BssSI 406 CACGAG CACGAG(-5/-1) 775 CACGAG DraII 83 AGGACCC [GA]G^G[ACGT]CC[CT] 1076 AGGCCCT EcoO109I 83 AGGACCC [GA]G^G[ACGT]CC[CT] 1076 AGGCCCT EcoT14I 419 CCTTGG C^C[AT][AT]GG 477 CCTTGG FauI 1116 CCCGC CCCGC(4/6) 246 GCGGG HaeII 446 AGCGCC [GA]GCGC^[CT] 464 AGCGCC Hin1I 202 GGCGTC G[GA]^CG[CT]C 604 GACGTC MroI 20 TCCGGA T^CCGGA 847 TCCGGA MseI 386 TTAA T^TAA 809 TTAA PvuII 1021 CAGCTG CAG^CTG 1099 CAGCTG RsaI 39 GTAC GT^AC 1221 GTAC StyI 419 CCTTGG C^C[AT][AT]GG 477 CCTTGG TaqI 35 TCGA T^CGA 1037 TCGA TfiI 60 GATTC G^A[AT]TC 143 GAATC Tru9I 386 TTAA T^TAA 809 TTAA TthHB8I 35 TCGA T^CGA 1037 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AciI AluI AlwI AvaII BalI BbvI BmyI BpmI BsaJI BsaWI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EcoRII Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PflMI PleI Sau3AI Sau96I ScrFI SfaNI SmlI TseI Tsp45I TspRI Van91I XhoII