Restriction map for
pFN21AE3994
(Circular) (Six-base) MAPSORT of: pFN21AE3994.seq Check: 2499 from: 1 to: 5968
pFN21AE3994 5968 bp
With 182 enzymes: SgfI *
March 28, 2012 11:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2657 2657
Size: 5968
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2613 3778 278
Size: 53 83 186 936 1077 1165 2468
Fragments arranged by size:
2468 1165 1077 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3311 1937
Size: 1374 4594
Acc65I G'GTAC_C
Cuts at: 3225 3289 3225
Size: 64 5904
AclI AA'CG_TT
Cuts at: 1563 4095 4468 1563
Size: 2532 373 3063
Fragments arranged by size:
3063 2532 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2825 3149 3239 4031 5415 5683 1632
Size: 1193 324 90 792 1384 268 1917
Fragments arranged by size:
1917 1384 1193 792 324 268 90
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5771
AflIII A'CryG_T
Cuts at: 1203 4867 5844 1203
Size: 3664 977 1327
Fragments arranged by size:
3664 1327 977
AgeI A'CCGG_T
Cuts at: 4799 4799
Size: 5968
AhdI GACnn_n'nnGTC
Cuts at: 4697 4697
Size: 5968
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5968
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2237 1184
Size: 1053 4915
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2627 2659 2627
Size: 32 5936
AlwNI CAG_nnn'CTG
Cuts at: 2053 3711 5283 2053
Size: 1658 1572 2738
Fragments arranged by size:
2738 1658 1572
ApaLI G'TGCA_C
Cuts at: 4025 5181 4025
Size: 1156 4812
ApoI r'AATT_y
Cuts at: 1493 3277 3503 3655 5764 1493
Size: 1784 226 152 2109 1697
Fragments arranged by size:
2109 1784 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4522 160
Size: 872 3490 1606
Fragments arranged by size:
3490 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5968
AvaI C'yCGr_G
Cuts at: 1870 1943 2093 3293 1870
Size: 73 150 1200 4545
Fragments arranged by size:
4545 1200 150 73
BamHI G'GATC_C
Cuts at: 2082 2406 3298 3665 2082
Size: 324 892 367 4385
Fragments arranged by size:
4385 892 367 324
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3225 3289 4744 618
Size: 325 217 627 1438 64 1455 1842
Fragments arranged by size:
1842 1455 1438 627 325 217 64
BanII G_rGCy'C
Cuts at: 1391 2075 3287 1391
Size: 684 1212 4072
Fragments arranged by size:
4072 1212 684
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2304 2955 928
Size: 971 405 651 3941
Fragments arranged by size:
3941 971 651 405
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2942 2976 4159 4193 1903
Size: 34 50 34 921 34 1183 34 3678
Fragments arranged by size:
3678 1183 921 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3860 5070 3860
Size: 1210 4758
BclI T'GATC_A
Cuts at: 1548 2706 1548
Size: 1158 4810
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3214 4579 136
Size: 107 122 71 631 605 1542 1365 1525
Fragments arranged by size:
1542 1525 1365 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5963 5963
Size: 5968
BlpI GC'TnA_GC
Cuts at: 2235 3385 2235
Size: 1150 4818
Bme1580I G_kGCm'C
Cuts at: 1790 2774 4029 5185 1790
Size: 984 1255 1156 2573
Fragments arranged by size:
2573 1255 1156 984
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5968
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2115 2297 3699
Size: 416 53 83 399 24 698 182 1402
Cuts at: 3699 4657 442
Size: 958 1753
Fragments arranged by size:
1753 1402 958 698 416 399 182 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5968
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2192 2867 3008 3128 4628 1239
Size: 953 675 141 120 1500 2579
Fragments arranged by size:
2579 1500 953 675 141 120
Bpu10I CC'TnA_GC
Cuts at: 2448 3233 2448
Size: 785 5183
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2210 3113 3118 4093 4958 5256 2210
Size: 903 5 975 865 298 2922
Fragments arranged by size:
2922 975 903 865 298 5
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2666 4631 882
Size: 617 1167 1965 2219
Fragments arranged by size:
2219 1965 1167 617
BsaAI yAC'GTr
Cuts at: 493 1204 3759 493
Size: 711 2555 2702
Fragments arranged by size:
2702 2555 711
BsaBI GATnn'nnATC
Cuts at: 5962 5962
Size: 5968
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2208 2610 3775 4157
Size: 53 83 186 936 675 402 1165 382
Cuts at: 4157 4792 275
Size: 635 1451
Fragments arranged by size:
1451 1165 936 675 635 402 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2025 2403 2852 4401 4799 5073 5220 5631 2025
Size: 378 449 1549 398 274 147 411 2362
Fragments arranged by size:
2362 1549 449 411 398 378 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2478 2508 2855 2885 3233
Size: 30 165 30 1075 30 347 30 348
Cuts at: 3233 3263 1178
Size: 30 3883
Fragments arranged by size:
3883 1075 348 347 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2153 2456 2708 1186
Size: 967 303 252 4446
Fragments arranged by size:
4446 967 303 252
BseYI C'CCAG_C
Cuts at: 2239 2762 2773 5171 5667 2239
Size: 523 11 2398 496 2540
Fragments arranged by size:
2540 2398 523 496 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2000 2579 2804 2861 2926 1163
Size: 573 264 579 225 57 65 4205
Fragments arranged by size:
4205 579 573 264 225 65 57
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2259 3418 4179 5207 1540
Size: 275 186 258 1159 761 1028 2301
Fragments arranged by size:
2301 1159 1028 761 275 258 186
BsiHKAI G_wGCw'C
Cuts at: 3243 4029 4114 5185 3243
Size: 786 85 1071 4026
Fragments arranged by size:
4026 1071 786 85
BsmI GAATG_Cn'
Cuts at: 3479 3572 3479
Size: 93 5875
BsmBI CGTCTCn'nnnn_
Cuts at: 2591 2591
Size: 5968
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2075 2774 3243 3287 4029 4114 5185
Size: 399 285 699 469 44 742 85 1071
Cuts at: 5185 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 699 469 399 285 85
44
BspEI T'CCGG_A
Cuts at: 2025 2852 5631 2025
Size: 827 2779 2362
Fragments arranged by size:
2779 2362 827
BspHI T'CATG_A
Cuts at: 3857 5587 3857
Size: 1730 4238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2657 3323 844
Size: 1813 666 3489
Fragments arranged by size:
3489 1813 666
BsrBI CCG'CTC
Cuts at: 1394 1823 2098 3855 1394
Size: 429 275 1757 3507
Fragments arranged by size:
3507 1757 429 275
BsrDI GCAATG_nn'
Cuts at: 65 1248 4463 4637 65
Size: 1183 3215 174 1396
Fragments arranged by size:
3215 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2115 4612 4799 1700
Size: 110 305 2497 187 2869
Fragments arranged by size:
2869 2497 305 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5968
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5968
BssSI C'ACGA_G
Cuts at: 2411 2780 4028 5040 2411
Size: 369 1248 1012 3339
Fragments arranged by size:
3339 1248 1012 369
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5968
BstBI TT'CG_AA
Cuts at: 4772 4772
Size: 5968
BstEII G'GTnAC_C
Cuts at: 1184 3004 1184
Size: 1820 4148
BstYI r'GATC_y
Cuts at: 1972 2082 2406 2562 2995 3075 3298 3665 4050
Size: 110 324 156 433 80 223 367 385
Cuts at: 4050 4067 5519 5605 5617 5963 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 433 385 367 346 324 223
156 110 86 80 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2676 3170 3252 513
Size: 552 1611 494 82 3229
Fragments arranged by size:
3229 1611 552 494 82
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2314 487
Size: 683 56 1088 4141
Fragments arranged by size:
4141 1088 683 56
BtsI GCAGTG_nn'
Cuts at: 3484 4278 4298 3484
Size: 794 20 5154
Fragments arranged by size:
5154 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5968
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5933
DraI TTT'AAA
Cuts at: 2815 3272 2815
Size: 457 5511
DraIII CAC_nnn'GTG
Cuts at: 3055 3055
Size: 5968
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4975 809
Size: 507 3659 1802
Fragments arranged by size:
3659 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2256 2433 2541
Size: 54 1425 50 275 18 426 177 108
Cuts at: 2541 3147 3415 4304 5933 8
Size: 606 268 889 1629 43
Fragments arranged by size:
1629 1425 889 606 426 275 268 177
108 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2256 3415 1537
Size: 275 444 1159 4090
Fragments arranged by size:
4090 1159 444 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2801 3898 1353
Size: 1448 1097 3423
Fragments arranged by size:
3423 1448 1097
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3083 4555 4929 5075 387
Size: 1064 138 1494 1472 374 146 1280
Fragments arranged by size:
1494 1472 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2192 2825 2867 3008 3128 3149 3239
Size: 393 560 633 42 141 120 21 90
Cuts at: 3239 4031 4628 5415 5683 1239
Size: 792 597 787 268 1524
Fragments arranged by size:
1524 792 787 633 597 560 393 268
141 120 90 42 21
EcoO109I rG'GnC_Cy
Cuts at: 2089 3082 2089
Size: 993 4975
EcoRI G'AATT_C
Cuts at: 3277 3655 3277
Size: 378 5590
EcoRV GAT'ATC
Cuts at: 3046 3046
Size: 5968
FspI TGC'GCA
Cuts at: 2975 4474 2975
Size: 1499 4469
HaeII r_GCGC'y
Cuts at: 1678 2455 2473 5115 1678
Size: 777 18 2642 2531
Fragments arranged by size:
2642 2531 777 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2478 2510 2998 3030 4609
Size: 32 135 32 906 32 488 32 1579
Cuts at: 4609 4641 4683 4715 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1579 906 488 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3312 3558 669
Size: 988 281 1374 246 3079
Fragments arranged by size:
3079 1374 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5968
HpaI GTT'AAC
Cuts at: 3558 3558
Size: 5968
KpnI G_GTAC'C
Cuts at: 3229 3293 3229
Size: 64 5904
MfeI C'AATT_G
Cuts at: 3567 3567
Size: 5968
MluI A'CGCG_T
Cuts at: 5844 5844
Size: 5968
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5082 5266 1305
Size: 3777 184 2007
Fragments arranged by size:
3777 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2435 2543 3149 10
Size: 54 1425 343 603 108 606 2829
Fragments arranged by size:
2829 1425 606 603 343 108 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3926 4285 4444 518
Size: 648 2760 359 159 2042
Fragments arranged by size:
2760 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2053 2504 3028 3106 3384 4061 5209 5454
Size: 447 451 524 78 278 677 1148 245
Cuts at: 5454 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 524 451 447 278 245
78
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5416
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5968
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5968
NotI GC'GGCC_GC
Cuts at: 3415 3415
Size: 5968
NspI r_CATG'y
Cuts at: 1132 1229 3326 4871 1132
Size: 97 2097 1545 2229
Fragments arranged by size:
2229 2097 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2077 1348
Size: 729 5239
PciI A'CATG_T
Cuts at: 4867 4867
Size: 5968
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2053 2539 3055 1108
Size: 945 486 516 4021
Fragments arranged by size:
4021 945 516 486
PfoI T'CCnGG_A
Cuts at: 2085 2475 2844 2991 2085
Size: 390 369 147 5062
Fragments arranged by size:
5062 390 369 147
PmeI GTTT'AAAC
Cuts at: 3272 3272
Size: 5968
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2911 2943 4037 4069 5550 5582 2911
Size: 32 1094 32 1481 32 3297
Fragments arranged by size:
3297 1481 1094 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2089 2089
Size: 5968
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5968
PsiI TTA'TAA
Cuts at: 3538 3538
Size: 5968
PstI C_TGCA'G
Cuts at: 830 2469 3320 830
Size: 1639 851 3478
Fragments arranged by size:
3478 1639 851
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5968
PvuII CAG'CTG
Cuts at: 3028 3106 3028
Size: 78 5890
SalI G'TCGA_C
Cuts at: 1936 3310 1936
Size: 1374 4594
SapI GCTCTTCn'nnn_
Cuts at: 2801 2801
Size: 5968
SbfI CC_TGCA'GG
Cuts at: 3320 3320
Size: 5968
ScaI AGT'ACT
Cuts at: 4216 5928 4216
Size: 1712 4256
SfcI C'TryA_G
Cuts at: 826 1045 2465 3316 4451 5132 5323 826
Size: 219 1420 851 1135 681 191 1471
Fragments arranged by size:
1471 1420 1135 851 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5968
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5968
SmaI CCC'GGG
Cuts at: 1872 3295 1872
Size: 1423 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2225 3097 3128 4072 4973 5235
Size: 197 926 282 872 31 944 901 262
Cuts at: 5235 820
Size: 1553
Fragments arranged by size:
1553 944 926 901 872 282 262 197
31
SnaBI TAC'GTA
Cuts at: 493 3759 493
Size: 3266 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5968
SphI G_CATG'C
Cuts at: 1132 3326 1132
Size: 2194 3774
SspI AAT'ATT
Cuts at: 5 52 3892 5
Size: 47 3840 2081
Fragments arranged by size:
3840 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5968
StyI C'CwwG_G
Cuts at: 513 1065 2424 2482 3407 513
Size: 552 1359 58 925 3074
Fragments arranged by size:
3074 1359 925 552 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2807 2949 3995 4012 4165 4350 4795
Size: 1255 1227 142 1046 17 153 185 445
Cuts at: 4795 325
Size: 1498
Fragments arranged by size:
1498 1255 1227 1046 445 185 153 142
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4214 5926 96
Size: 274 80 33 51 1443 2237 1712 138
Fragments arranged by size:
2237 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2870 2902 3233 3265 5928 5960 2870
Size: 32 331 32 2663 32 2878
Fragments arranged by size:
2878 2663 331 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2303 1316
Size: 987 4981
XbaI T'CTAG_A
Cuts at: 3304 3304
Size: 5968
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5968
XmaI C'CCGG_G
Cuts at: 1870 3293 1870
Size: 1423 4545
XmnI GAAnn'nnTTC
Cuts at: 4097 4097
Size: 5968
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2611 3776 276
Size: 53 83 186 936 1077 1165 2468
Fragments arranged by size:
2468 1165 1077 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI