(Linear) (Six-base) MAPSORT of: KIEE3994.seq Check: 8124 from: 1 to: 1263 KIEE3994.orf With 182 enzymes: SgfI * March 22, 2012 17:06 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 652 1263 Size: 652 611 AatII G_ACGT'C Cuts at: 0 608 1263 Size: 608 655 Acc65I G'GTAC_C Cuts at: 0 1220 1263 Size: 1220 43 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 820 1144 1234 1263 Size: 820 324 90 29 Fragments arranged by size: 820 324 90 29 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 232 1263 Size: 232 1031 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 622 654 1263 Size: 622 32 609 Fragments arranged by size: 622 609 32 AlwNI CAG_nnn'CTG Cuts at: 0 48 1263 Size: 48 1215 AvaI C'yCGr_G Cuts at: 0 88 1263 Size: 88 1175 BamHI G'GATC_C Cuts at: 0 77 401 1263 Size: 77 324 862 Fragments arranged by size: 862 324 77 BanI G'GyrC_C Cuts at: 0 1220 1263 Size: 1220 43 BanII G_rGCy'C Cuts at: 0 70 1263 Size: 70 1193 BbsI GAAGACnn'nnnn_ Cuts at: 0 299 950 1263 Size: 299 651 313 Fragments arranged by size: 651 313 299 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 937 971 1263 Size: 937 34 292 Fragments arranged by size: 937 292 34 BclI T'GATC_A Cuts at: 0 701 1263 Size: 701 562 BglI GCCn_nnn'nGGC Cuts at: 0 1209 1263 Size: 1209 54 BlpI GC'TnA_GC Cuts at: 0 230 1263 Size: 230 1033 Bme1580I G_kGCm'C Cuts at: 0 769 1263 Size: 769 494 BmrI ACTGGGnnnn_n' Cuts at: 0 110 292 1263 Size: 110 182 971 Fragments arranged by size: 971 182 110 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 187 862 1003 1123 1263 Size: 187 675 141 120 140 Fragments arranged by size: 675 187 141 140 120 Bpu10I CC'TnA_GC Cuts at: 0 443 1228 1263 Size: 443 785 35 Fragments arranged by size: 785 443 35 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 205 1108 1113 1263 Size: 205 903 5 150 Fragments arranged by size: 903 205 150 5 BsaI GGTCTCn'nnnn_ Cuts at: 0 661 1263 Size: 661 602 BsaHI Gr'CG_yC Cuts at: 0 203 605 1263 Size: 203 402 658 Fragments arranged by size: 658 402 203 BsaWI w'CCGG_w Cuts at: 0 20 398 847 1263 Size: 20 378 449 416 Fragments arranged by size: 449 416 378 20 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 473 503 850 880 1228 1258 1263 Size: 473 30 347 30 348 30 5 Fragments arranged by size: 473 348 347 30 30 30 5 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 148 451 703 1263 Size: 148 303 252 560 Fragments arranged by size: 560 303 252 148 BseYI C'CCAG_C Cuts at: 0 234 757 768 1263 Size: 234 523 11 495 Fragments arranged by size: 523 495 234 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 574 799 856 921 1263 Size: 574 225 57 65 342 Fragments arranged by size: 574 342 225 65 57 BsiEI CG_ry'CG Cuts at: 0 254 1263 Size: 254 1009 BsiHKAI G_wGCw'C Cuts at: 0 1238 1263 Size: 1238 25 BsmBI CGTCTCn'nnnn_ Cuts at: 0 586 1263 Size: 586 677 Bsp1286I G_dGCh'C Cuts at: 0 70 769 1238 1263 Size: 70 699 469 25 Fragments arranged by size: 699 469 70 25 BspEI T'CCGG_A Cuts at: 0 20 847 1263 Size: 20 827 416 Fragments arranged by size: 827 416 20 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 652 1263 Size: 652 611 BsrBI CCG'CTC Cuts at: 0 93 1263 Size: 93 1170 BsrFI r'CCGG_y Cuts at: 0 110 1263 Size: 110 1153 BssSI C'ACGA_G Cuts at: 0 406 775 1263 Size: 406 369 488 Fragments arranged by size: 488 406 369 BstEII G'GTnAC_C Cuts at: 0 999 1263 Size: 999 264 BstYI r'GATC_y Cuts at: 0 77 401 557 990 1070 1263 Size: 77 324 156 433 80 193 Fragments arranged by size: 433 324 193 156 80 77 BtgI C'CryG_G Cuts at: 0 671 1165 1247 1263 Size: 671 494 82 16 Fragments arranged by size: 671 494 82 16 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 309 1263 Size: 309 954 DraI TTT'AAA Cuts at: 0 810 1263 Size: 810 453 DraIII CAC_nnn'GTG Cuts at: 0 1050 1263 Size: 1050 213 EaeI y'GGCC_r Cuts at: 0 251 428 536 1142 1263 Size: 251 177 108 606 121 Fragments arranged by size: 606 251 177 121 108 EagI C'GGCC_G Cuts at: 0 251 1263 Size: 251 1012 EarI CTCTTCn'nnn_ Cuts at: 0 796 1263 Size: 796 467 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1078 1263 Size: 1078 185 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 187 820 862 1003 1123 1144 1234 1263 Size: 187 633 42 141 120 21 90 29 Fragments arranged by size: 633 187 141 120 90 42 29 21 EcoO109I rG'GnC_Cy Cuts at: 0 84 1077 1263 Size: 84 993 186 Fragments arranged by size: 993 186 84 EcoRV GAT'ATC Cuts at: 0 1041 1263 Size: 1041 222 FspI TGC'GCA Cuts at: 0 970 1263 Size: 970 293 HaeII r_GCGC'y Cuts at: 0 450 468 1263 Size: 450 18 795 Fragments arranged by size: 795 450 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 473 505 993 1025 1263 Size: 473 32 488 32 238 Fragments arranged by size: 488 473 238 32 32 KpnI G_GTAC'C Cuts at: 0 1224 1263 Size: 1224 39 MscI TGG'CCA Cuts at: 0 430 538 1144 1263 Size: 430 108 606 119 Fragments arranged by size: 606 430 119 108 MspA1I CmG'CkG Cuts at: 0 48 499 1023 1101 1263 Size: 48 451 524 78 162 Fragments arranged by size: 524 451 162 78 48 PasI CC'CwG_GG Cuts at: 0 72 1263 Size: 72 1191 PflMI CCAn_nnn'nTGG Cuts at: 0 48 534 1050 1263 Size: 48 486 516 213 Fragments arranged by size: 516 486 213 48 PfoI T'CCnGG_A Cuts at: 0 80 470 839 986 1263 Size: 80 390 369 147 277 Fragments arranged by size: 390 369 277 147 80 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 906 938 1263 Size: 906 32 325 Fragments arranged by size: 906 325 32 PpuMI rG'GwC_Cy Cuts at: 0 84 1263 Size: 84 1179 PstI C_TGCA'G Cuts at: 0 464 1263 Size: 464 799 PvuII CAG'CTG Cuts at: 0 1023 1101 1263 Size: 1023 78 162 Fragments arranged by size: 1023 162 78 SapI GCTCTTCn'nnn_ Cuts at: 0 796 1263 Size: 796 467 SfcI C'TryA_G Cuts at: 0 460 1263 Size: 460 803 SmlI C'TyrA_G Cuts at: 0 220 1092 1123 1263 Size: 220 872 31 140 Fragments arranged by size: 872 220 140 31 StyI C'CwwG_G Cuts at: 0 419 477 1263 Size: 419 58 786 Fragments arranged by size: 786 419 58 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 802 944 1263 Size: 802 142 319 Fragments arranged by size: 802 319 142 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 865 897 1228 1260 1263 Size: 865 32 331 32 3 Fragments arranged by size: 865 331 32 32 3 Tth111I GACn'n_nGTC Cuts at: 0 298 1263 Size: 298 965 ZraI GAC'GTC Cuts at: 0 606 1263 Size: 606 657 Enzymes that do cut: AarI AatII Acc65I AcuI AlfI AloI AlwNI AvaI BamHI BanI BanII BbsI BcgI BclI BglI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstEII BstYI BtgI BtgZI DraI DraIII EaeI EagI EarI EciI Eco57MI EcoO109I EcoRV FspI HaeII Hin4I KpnI MscI MspA1I PasI PflMI PfoI PpiI PpuMI PstI PvuII SapI SfcI SmlI StyI TaqII TstI Tth111I ZraI Enzymes that do not cut: AccI AclI AfeI AflII AflIII AgeI AhdI AleI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BciVI BfrBI BglII BmgBI BmtI BplI BsaAI BsaBI BsiWI BsmI BspHI BsrDI BsrGI BssHII BstAPI BstBI BstXI BstZ17I Bsu36I BtsI ClaI CspCI DrdI EcoICRI EcoNI EcoRI FalI FseI FspAI HincII HindIII HpaI KasI MfeI MluI MmeI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI XbaI XcmI XhoI XmaI XmnI