Restriction map for
pFN21AE9280
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# NO CUT ENZYMES ( 127 enzymes) #
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Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AviII
AvrII BbeI BbrPI BbsI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaWI BseAI BsgI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI DraIII Eam1105I Eco47III
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KasI KpnI
MaeI MamI MfeI MluI MroI
MseI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI TaqI Tru9I Tth111I TthHB8I
XbaI XhoI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 864 GACGTC GACGT^C
AcsI 382 GAATTC [GA]^AATT[CT]
AcyI 864 GACGTC G[GA]^CG[CT]C
AocI 462 CCTGAGG CC^T[ACGT]AGG
ApoI 382 GAATTC [GA]^AATT[CT]
Asp700I 468 GAAGGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1032 TGGCCA TGG^CCA
BamHI 431 GGATCC G^GATCC
BanI 1114 GGTGCC G^G[CT][GA]CC
BbvCI 823 GCTGAGG CCTCAGC(-5/-2)
BcgI 747 GCAGGACCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 591 GCCCACCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 864 GACGTC G[GA]^CG[CT]C
BsaI 769 GGTCTC GGTCTC(1/5)
BsiEI 22 CGGCCG CG[GA][CT]^CG
BsmAI 770 GTCTC GTCTC(1/5)
BsmFI 862 GGGAC GGGAC(10/14)
BsrFI 289 GCCGGT [GA]^CCGG[CT]
BstXI 365 CCAAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 462 CCTGAGG CC^T[ACGT]AGG
Cfr10I 289 GCCGGT [GA]^CCGG[CT]
DrdI 1009 GACCTGTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 22 CGGCCG C^GGCCG
EarI 172 GAAGAG CTCTTC(1/4)
EclXI 22 CGGCCG C^GGCCG
Eco52I 22 CGGCCG C^GGCCG
Eco81I 462 CCTGAGG CC^T[ACGT]AGG
EcoRI 382 GAATTC G^AATTC
EcoT14I 726 CCATGG C^C[AT][AT]GG
HaeII 164 AGCGCC [GA]GCGC^[CT]
HgaI 543 GCGTC GACGC(5/10)
Hin1I 864 GACGTC G[GA]^CG[CT]C
HincII 619 GTCAAC GT[CT]^[GA]AC
HindII 619 GTCAAC GT[CT]^[GA]AC
HinfI 556 GAATC G^A[ACGT]TC
Ksp632I 172 GAAGAG CTCTTC(1/4)
KspI 204 CCGCGG CCGC^GG
MaeIII 679 GTGAC ^GT[ACGT]AC
MluNI 1032 TGGCCA TGG^CCA
MscI 1032 TGGCCA TGG^CCA
MslI 576 CATCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 726 CCATGG C^CATGG
PflMI 942 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 749 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 421 CAGCTG CAG^CTG
SacII 204 CCGCGG CCGC^GG
SfiI 590 GGCCCACCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 112 CTCAAG C^T[CT][GA]AG
SphI 915 GCATGC GCATG^C
StyI 726 CCATGG C^C[AT][AT]GG
TfiI 556 GAATC G^A[AT]TC
Tsp45I 679 GTGAC ^GT[GC]AC
Tsp509I 383 AATT ^AATT
Van91I 942 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 468 GAAGGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 11 enzymes) #
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AvaI 33 CCCGGG C^[CT]CG[GA]G
667 CCCGGG
AvaII 201 GGACC G^G[AT]CC
750 GGACC
BsoBI 33 CCCGGG C^[CT]CG[GA]G
667 CCCGGG
BsrBI 133 GAGCGG CCGCTC(-3/-3)
959 GAGCGG
MaeII 865 ACGT A^CGT
1016 ACGT
NspI 539 ACATGC [GA]CATG^[CT]
915 GCATGC
PstI 436 CTGCAG CTGCA^G
1090 CTGCAG
SmaI 33 CCCGGG CCC^GGG
667 CCCGGG
XcmI 314 CCATCTTCTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
974 CCAACCTGGAGGTGG
XmaCI 33 CCCGGG C^CCGGG
667 CCCGGG
XmaI 33 CCCGGG C^CCGGG
667 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoNI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MboI MboII
MflI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TseI TspRI XhoII