(Circular) (Six-base) MAPSORT of: pFN21AE9280.seq Check: 2257 from: 1 to: 5833 pFN21AE9280 5833 bp With 182 enzymes: SgfI * December 8, 2014 13:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2873 3643 278 Size: 53 83 186 936 1337 770 2468 Fragments arranged by size: 2468 1337 936 770 186 83 53 AccI GT'mk_AC Cuts at: 1937 3176 1937 Size: 1239 4594 Acc65I G'GTAC_C Cuts at: 3154 3154 Size: 5833 AclI AA'CG_TT Cuts at: 1563 3960 4333 1563 Size: 2397 373 3063 Fragments arranged by size: 3063 2397 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2899 3896 5280 5548 1632 Size: 1267 997 1384 268 1917 Fragments arranged by size: 1917 1384 1267 997 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5636 AflIII A'CryG_T Cuts at: 1203 4732 5709 1203 Size: 3529 977 1327 Fragments arranged by size: 3529 1327 977 AgeI A'CCGG_T Cuts at: 4664 4664 Size: 5833 AhdI GACnn_n'nnGTC Cuts at: 4562 4562 Size: 5833 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5833 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5833 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2552 2584 2988 3020 2552 Size: 32 404 32 5365 Fragments arranged by size: 5365 404 32 32 AlwNI CAG_nnn'CTG Cuts at: 2313 2449 2467 2953 3576 5148 2313 Size: 136 18 486 623 1572 2998 Fragments arranged by size: 2998 1572 623 486 136 18 ApaLI G'TGCA_C Cuts at: 3890 5046 3890 Size: 1156 4677 ApoI r'AATT_y Cuts at: 1493 2387 3142 3368 3520 5629 1493 Size: 894 755 226 152 2109 1697 Fragments arranged by size: 2109 1697 894 755 226 152 AseI AT'TA_AT Cuts at: 160 1032 4387 160 Size: 872 3355 1606 Fragments arranged by size: 3355 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5833 AvaI C'yCGr_G Cuts at: 1870 1943 2038 2672 3158 1870 Size: 73 95 634 486 4545 Fragments arranged by size: 4545 634 486 95 73 BamHI G'GATC_C Cuts at: 2436 3163 3530 2436 Size: 727 367 4739 Fragments arranged by size: 4739 727 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3119 3154 4609 618 Size: 325 217 627 1332 35 1455 1842 Fragments arranged by size: 1842 1455 1332 627 325 217 35 BanII G_rGCy'C Cuts at: 1391 2249 2517 2673 3152 1391 Size: 858 268 156 479 4072 Fragments arranged by size: 4072 858 479 268 156 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4862 BbvCI CC'TCA_GC Cuts at: 2829 2829 Size: 5833 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2741 2775 4024 4058 1903 Size: 34 804 34 1249 34 3678 Fragments arranged by size: 3678 1249 804 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3725 4935 3725 Size: 1210 4623 BclI T'GATC_A Cuts at: 1548 1548 Size: 5833 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2602 4444 136 Size: 107 122 71 631 605 930 1842 1525 Fragments arranged by size: 1842 1525 930 631 605 122 107 71 BglII A'GATC_T Cuts at: 5828 5828 Size: 5833 BlpI GC'TnA_GC Cuts at: 3250 3250 Size: 5833 Bme1580I G_kGCm'C Cuts at: 1790 2152 3124 3894 5050 1790 Size: 362 972 770 1156 2573 Fragments arranged by size: 2573 1156 972 770 362 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5833 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2791 2994 3564 Size: 416 53 83 399 24 1374 203 570 Cuts at: 3564 4522 442 Size: 958 1753 Fragments arranged by size: 1753 1374 958 570 416 399 203 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5833 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2899 2899 Size: 5833 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2471 2687 2930 2982 3005 4493 1239 Size: 917 315 216 243 52 23 1488 2579 Fragments arranged by size: 2579 1488 917 315 243 216 52 23 Bpu10I CC'TnA_GC Cuts at: 2829 2829 Size: 5833 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2102 3958 4823 5121 2102 Size: 1856 865 298 2814 Fragments arranged by size: 2814 1856 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2780 4496 882 Size: 617 1281 1716 2219 Fragments arranged by size: 2219 1716 1281 617 BsaAI yAC'GTr Cuts at: 493 1204 3624 493 Size: 711 2420 2702 Fragments arranged by size: 2702 2420 711 BsaBI GATnn'nnATC Cuts at: 5827 5827 Size: 5833 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2870 3640 4022 4657 Size: 53 83 186 936 1337 770 382 635 Cuts at: 4657 275 Size: 1451 Fragments arranged by size: 1451 1337 936 770 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4266 4664 4938 5085 5496 4266 Size: 398 274 147 411 4603 Fragments arranged by size: 4603 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5608 Fragments arranged by size: 5608 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2108 2195 2198 2369 2409 1186 Size: 922 87 3 171 40 4610 Fragments arranged by size: 4610 922 171 87 40 3 BseYI C'CCAG_C Cuts at: 2901 5036 5532 2901 Size: 2135 496 3202 Fragments arranged by size: 3202 2135 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5260 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2030 3283 4044 5072 1540 Size: 275 186 29 1253 761 1028 2301 Fragments arranged by size: 2301 1253 1028 761 275 186 29 BsiHKAI G_wGCw'C Cuts at: 3894 3979 5050 3894 Size: 85 1071 4677 Fragments arranged by size: 4677 1071 85 BsmI GAATG_Cn' Cuts at: 3344 3437 3344 Size: 93 5740 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2152 2249 2517 2673 3124 3152 3894 Size: 399 362 97 268 156 451 28 742 Cuts at: 3894 3979 5050 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 451 399 362 268 156 97 85 28 BspEI T'CCGG_A Cuts at: 5496 5496 Size: 5833 BspHI T'CATG_A Cuts at: 3722 5452 3722 Size: 1730 4103 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3188 844 Size: 2344 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2140 2966 3720 1394 Size: 429 317 826 754 3507 Fragments arranged by size: 3507 826 754 429 317 BsrDI GCAATG_nn' Cuts at: 65 1248 4328 4502 65 Size: 1183 3080 174 1396 Fragments arranged by size: 3080 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2294 4477 4664 1700 Size: 110 484 2183 187 2869 Fragments arranged by size: 2869 2183 484 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5833 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5833 BssSI C'ACGA_G Cuts at: 3893 4905 3893 Size: 1012 4821 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5833 BstBI TT'CG_AA Cuts at: 4637 4637 Size: 5833 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5833 BstXI CCAn_nnnn'nTGG Cuts at: 2377 2377 Size: 5833 BstYI r'GATC_y Cuts at: 1972 2113 2436 2497 3163 3530 3915 3932 5384 Size: 141 323 61 666 367 385 17 1452 Cuts at: 5384 5470 5482 5828 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 666 385 367 346 323 141 86 61 17 12 Bsu36I CC'TnA_GG Cuts at: 2468 2468 Size: 5833 BtgI C'CryG_G Cuts at: 513 1065 2004 2209 2611 2731 513 Size: 552 939 205 402 120 3615 Fragments arranged by size: 3615 939 552 402 205 120 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2733 487 Size: 683 56 1507 3587 Fragments arranged by size: 3587 1507 683 56 BtsI GCAGTG_nn' Cuts at: 3349 4143 4163 3349 Size: 794 20 5019 Fragments arranged by size: 5019 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5833 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5798 DraI TTT'AAA Cuts at: 3137 3137 Size: 5833 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3020 4840 809 Size: 507 1704 1820 1802 Fragments arranged by size: 1820 1802 1704 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2027 2050 2292 Size: 54 1425 50 275 18 197 23 242 Cuts at: 2292 2603 3037 3087 3280 4169 5798 8 Size: 311 434 50 193 889 1629 43 Fragments arranged by size: 1629 1425 889 434 311 275 242 197 193 54 50 50 43 23 18 EagI C'GGCC_G Cuts at: 1537 1812 2027 3280 1537 Size: 275 215 1253 4090 Fragments arranged by size: 4090 1253 275 215 EarI CTCTTCn'nnn_ Cuts at: 1353 2172 3763 1353 Size: 819 1591 3423 Fragments arranged by size: 3423 1591 819 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 4420 4794 4940 387 Size: 1064 138 435 2396 374 146 1280 Fragments arranged by size: 2396 1280 1064 435 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2471 2687 2899 2930 2982 3005 Size: 393 524 315 216 212 31 52 23 Cuts at: 3005 3896 4493 5280 5548 1239 Size: 891 597 787 268 1524 Fragments arranged by size: 1524 891 787 597 524 393 315 268 216 212 52 31 23 EcoNI CCTnn'n_nnAGG Cuts at: 2177 2471 3110 2177 Size: 294 639 4900 Fragments arranged by size: 4900 639 294 EcoO109I rG'GnC_Cy Cuts at: 2755 2833 2941 2755 Size: 78 108 5647 Fragments arranged by size: 5647 108 78 EcoRI G'AATT_C Cuts at: 2387 3142 3520 2387 Size: 755 378 4700 Fragments arranged by size: 4700 755 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2497 2497 Size: 5833 FspI TGC'GCA Cuts at: 4339 4339 Size: 5833 HaeII r_GCGC'y Cuts at: 1678 2173 4980 1678 Size: 495 2807 2531 Fragments arranged by size: 2807 2531 495 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2546 2565 2578 2597 4474 Size: 32 135 32 974 19 13 19 1877 Cuts at: 4474 4506 4548 4580 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1877 974 135 42 32 32 32 32 19 19 13 HincII GTy'rAC Cuts at: 669 1657 1938 2626 3177 3423 669 Size: 988 281 688 551 246 3079 Fragments arranged by size: 3079 988 688 551 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5833 HpaI GTT'AAC Cuts at: 3423 3423 Size: 5833 KpnI G_GTAC'C Cuts at: 3158 3158 Size: 5833 MfeI C'AATT_G Cuts at: 3432 3432 Size: 5833 MluI A'CGCG_T Cuts at: 5709 5709 Size: 5833 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2821 4947 5131 1305 Size: 1516 2126 184 2007 Fragments arranged by size: 2126 2007 1516 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3039 10 Size: 54 1425 343 1207 2804 Fragments arranged by size: 2804 1425 1207 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2585 3791 4150 4309 518 Size: 648 1419 1206 359 159 2042 Fragments arranged by size: 2042 1419 1206 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2146 2192 2211 2428 3249 3926 5074 5319 Size: 540 46 19 217 821 677 1148 245 Cuts at: 5319 1606 Size: 2120 Fragments arranged by size: 2120 1148 821 677 540 245 217 46 19 NcoI C'CATG_G Cuts at: 513 1065 2004 2731 513 Size: 552 939 727 3615 Fragments arranged by size: 3615 939 727 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5833 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5833 NotI GC'GGCC_GC Cuts at: 3280 3280 Size: 5833 NspI r_CATG'y Cuts at: 1132 1229 2548 2924 3191 4736 1132 Size: 97 1319 376 267 1545 2229 Fragments arranged by size: 2229 1545 1319 376 267 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5833 PciI A'CATG_T Cuts at: 4732 4732 Size: 5833 PflMI CCAn_nnn'nTGG Cuts at: 1108 2953 1108 Size: 1845 3988 PfoI T'CCnGG_A Cuts at: 2253 2253 Size: 5833 PmeI GTTT'AAAC Cuts at: 3137 3137 Size: 5833 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3902 3934 5415 5447 3902 Size: 32 1481 32 4288 Fragments arranged by size: 4288 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2755 2755 Size: 5833 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5833 PsiI TTA'TAA Cuts at: 3403 3403 Size: 5833 PstI C_TGCA'G Cuts at: 830 2445 3099 3185 830 Size: 1615 654 86 3478 Fragments arranged by size: 3478 1615 654 86 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5833 PvuII CAG'CTG Cuts at: 2428 2428 Size: 5833 SacII CC_GC'GG Cuts at: 2212 2212 Size: 5833 SalI G'TCGA_C Cuts at: 1936 3175 1936 Size: 1239 4594 SbfI CC_TGCA'GG Cuts at: 3185 3185 Size: 5833 ScaI AGT'ACT Cuts at: 4081 5793 4081 Size: 1712 4121 SfcI C'TryA_G Cuts at: 826 1045 2441 2501 3095 3181 4316 4997 5188 Size: 219 1396 60 594 86 1135 681 191 Cuts at: 5188 826 Size: 1471 Fragments arranged by size: 1471 1396 1135 681 594 219 191 86 60 SfiI GGCCn_nnn'nGGCC Cuts at: 2602 2602 Size: 5833 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5833 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5833 SmaI CCC'GGG Cuts at: 1872 2040 2674 3160 1872 Size: 168 634 486 4545 Fragments arranged by size: 4545 634 486 168 SmlI C'TyrA_G Cuts at: 820 1017 1943 2117 3937 4838 5100 820 Size: 197 926 174 1820 901 262 1553 Fragments arranged by size: 1820 1553 926 901 262 197 174 SnaBI TAC'GTA Cuts at: 493 3624 493 Size: 3131 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5833 SphI G_CATG'C Cuts at: 1132 2924 3191 1132 Size: 1792 267 3774 Fragments arranged by size: 3774 1792 267 SrfI GCCC'GGGC Cuts at: 2674 2674 Size: 5833 SspI AAT'ATT Cuts at: 5 52 3757 5 Size: 47 3705 2081 Fragments arranged by size: 3705 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5833 StyI C'CwwG_G Cuts at: 513 1065 2004 2731 3272 513 Size: 552 939 727 541 3074 Fragments arranged by size: 3074 939 727 552 541 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2727 3860 3877 4030 4215 4660 325 Size: 1255 1147 1133 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1147 1133 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4079 5791 96 Size: 274 80 33 51 1443 2102 1712 138 Fragments arranged by size: 2102 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5793 5825 5793 Size: 32 5801 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5833 XbaI T'CTAG_A Cuts at: 3169 3169 Size: 5833 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2326 2986 2326 Size: 660 5173 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5833 XmaI C'CCGG_G Cuts at: 1870 2038 2672 3158 1870 Size: 168 634 486 4545 Fragments arranged by size: 4545 634 486 168 XmnI GAAnn'nnTTC Cuts at: 2477 3962 2477 Size: 1485 4348 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2871 3641 276 Size: 53 83 186 936 1337 770 2468 Fragments arranged by size: 2468 1337 936 770 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfoI SwaI