(Linear) (Six-base) MAPSORT of: KIEE9280.seq Check: 1788 from: 1 to: 1128 KIEE9280.orf With 182 enzymes: SgfI * December 5, 2014 13:29 .. AatII G_ACGT'C Cuts at: 0 868 1128 Size: 868 260 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 894 1128 Size: 894 234 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 547 579 983 1015 1128 Size: 547 32 404 32 113 Fragments arranged by size: 547 404 113 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 308 444 462 948 1128 Size: 308 136 18 486 180 Fragments arranged by size: 486 308 180 136 18 ApoI r'AATT_y Cuts at: 0 382 1128 Size: 382 746 AvaI C'yCGr_G Cuts at: 0 33 667 1128 Size: 33 634 461 Fragments arranged by size: 634 461 33 BamHI G'GATC_C Cuts at: 0 431 1128 Size: 431 697 BanI G'GyrC_C Cuts at: 0 1114 1128 Size: 1114 14 BanII G_rGCy'C Cuts at: 0 244 512 668 1128 Size: 244 268 156 460 Fragments arranged by size: 460 268 244 156 BbvCI CC'TCA_GC Cuts at: 0 824 1128 Size: 824 304 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 736 770 1128 Size: 736 34 358 Fragments arranged by size: 736 358 34 BglI GCCn_nnn'nGGC Cuts at: 0 597 1128 Size: 597 531 Bme1580I G_kGCm'C Cuts at: 0 147 1119 1128 Size: 147 972 9 Fragments arranged by size: 972 147 9 BmrI ACTGGGnnnn_n' Cuts at: 0 786 989 1128 Size: 786 203 139 Fragments arranged by size: 786 203 139 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 894 1128 Size: 894 234 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 151 466 682 925 977 1000 1128 Size: 151 315 216 243 52 23 128 Fragments arranged by size: 315 243 216 151 128 52 23 Bpu10I CC'TnA_GC Cuts at: 0 824 1128 Size: 824 304 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 97 1128 Size: 97 1031 BsaI GGTCTCn'nnnn_ Cuts at: 0 775 1128 Size: 775 353 BsaHI Gr'CG_yC Cuts at: 0 865 1128 Size: 865 263 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 103 190 193 364 404 1128 Size: 103 87 3 171 40 724 Fragments arranged by size: 724 171 103 87 40 3 BseYI C'CCAG_C Cuts at: 0 896 1128 Size: 896 232 BsiEI CG_ry'CG Cuts at: 0 25 1128 Size: 25 1103 Bsp1286I G_dGCh'C Cuts at: 0 147 244 512 668 1119 1128 Size: 147 97 268 156 451 9 Fragments arranged by size: 451 268 156 147 97 9 BsrBI CCG'CTC Cuts at: 0 135 961 1128 Size: 135 826 167 Fragments arranged by size: 826 167 135 BsrFI r'CCGG_y Cuts at: 0 289 1128 Size: 289 839 BstXI CCAn_nnnn'nTGG Cuts at: 0 372 1128 Size: 372 756 BstYI r'GATC_y Cuts at: 0 108 431 492 1128 Size: 108 323 61 636 Fragments arranged by size: 636 323 108 61 Bsu36I CC'TnA_GG Cuts at: 0 463 1128 Size: 463 665 BtgI C'CryG_G Cuts at: 0 204 606 726 1128 Size: 204 402 120 402 Fragments arranged by size: 402 402 204 120 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 728 1128 Size: 728 400 DrdI GACnn_nn'nnGTC Cuts at: 0 1015 1128 Size: 1015 113 EaeI y'GGCC_r Cuts at: 0 22 45 287 598 1032 1082 1128 Size: 22 23 242 311 434 50 46 Fragments arranged by size: 434 311 242 50 46 23 22 EagI C'GGCC_G Cuts at: 0 22 1128 Size: 22 1106 EarI CTCTTCn'nnn_ Cuts at: 0 167 1128 Size: 167 961 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 19 1128 Size: 19 1109 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 151 466 682 894 925 977 1000 1128 Size: 151 315 216 212 31 52 23 128 Fragments arranged by size: 315 216 212 151 128 52 31 23 EcoNI CCTnn'n_nnAGG Cuts at: 0 172 466 1105 1128 Size: 172 294 639 23 Fragments arranged by size: 639 294 172 23 EcoO109I rG'GnC_Cy Cuts at: 0 750 828 936 1128 Size: 750 78 108 192 Fragments arranged by size: 750 192 108 78 EcoRI G'AATT_C Cuts at: 0 382 1128 Size: 382 746 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 492 1128 Size: 492 636 HaeII r_GCGC'y Cuts at: 0 168 1128 Size: 168 960 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 541 560 573 592 1128 Size: 541 19 13 19 536 Fragments arranged by size: 541 536 19 19 13 HincII GTy'rAC Cuts at: 0 621 1128 Size: 621 507 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 816 1128 Size: 816 312 MscI TGG'CCA Cuts at: 0 1034 1128 Size: 1034 94 MslI CAynn'nnrTG Cuts at: 0 580 1128 Size: 580 548 MspA1I CmG'CkG Cuts at: 0 141 187 206 423 1128 Size: 141 46 19 217 705 Fragments arranged by size: 705 217 141 46 19 NcoI C'CATG_G Cuts at: 0 726 1128 Size: 726 402 NspI r_CATG'y Cuts at: 0 543 919 1128 Size: 543 376 209 Fragments arranged by size: 543 376 209 PflMI CCAn_nnn'nTGG Cuts at: 0 948 1128 Size: 948 180 PfoI T'CCnGG_A Cuts at: 0 248 1128 Size: 248 880 PpuMI rG'GwC_Cy Cuts at: 0 750 1128 Size: 750 378 PstI C_TGCA'G Cuts at: 0 440 1094 1128 Size: 440 654 34 Fragments arranged by size: 654 440 34 PvuII CAG'CTG Cuts at: 0 423 1128 Size: 423 705 SacII CC_GC'GG Cuts at: 0 207 1128 Size: 207 921 SfcI C'TryA_G Cuts at: 0 436 496 1090 1128 Size: 436 60 594 38 Fragments arranged by size: 594 436 60 38 SfiI GGCCn_nnn'nGGCC Cuts at: 0 597 1128 Size: 597 531 SmaI CCC'GGG Cuts at: 0 35 669 1128 Size: 35 634 459 Fragments arranged by size: 634 459 35 SmlI C'TyrA_G Cuts at: 0 112 1128 Size: 112 1016 SphI G_CATG'C Cuts at: 0 919 1128 Size: 919 209 SrfI GCCC'GGGC Cuts at: 0 669 1128 Size: 669 459 StyI C'CwwG_G Cuts at: 0 726 1128 Size: 726 402 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 722 1128 Size: 722 406 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 321 981 1128 Size: 321 660 147 Fragments arranged by size: 660 321 147 XmaI C'CCGG_G Cuts at: 0 33 667 1128 Size: 33 634 461 Fragments arranged by size: 634 461 33 XmnI GAAnn'nnTTC Cuts at: 0 472 1128 Size: 472 656 ZraI GAC'GTC Cuts at: 0 866 1128 Size: 866 262 Enzymes that do cut: AatII AcuI AloI AlwNI ApoI AvaI BamHI BanI BanII BbvCI BcgI BglI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BseRI BseYI BsiEI Bsp1286I BsrBI BsrFI BstXI BstYI Bsu36I BtgI BtgZI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII MmeI MscI MslI MspA1I NcoI NspI PflMI PfoI PpuMI PstI PvuII SacII SfcI SfiI SmaI SmlI SphI SrfI StyI TaqII XcmI XmaI XmnI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbsI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BsaAI BsaBI BsaWI BsaXI BsgI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtsI ClaI CspCI DraI DraIII EcoICRI EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfoI SgfI SgrAI SnaBI SpeI SspI StuI SwaI TatI TstI Tth111I XbaI XhoI