(Circular) (Six-base) MAPSORT of: pFN21AE3319.seq Check: 6227 from: 1 to: 7372 pFN21AE3319 7372 bp With 182 enzymes: SgfI * March 28, 2012 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3037 4406 4597 3037 Size: 1369 191 5812 Fragments arranged by size: 5812 1369 191 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5182 278 Size: 53 83 186 936 3646 2468 Fragments arranged by size: 3646 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4715 1937 Size: 2778 4594 Acc65I G'GTAC_C Cuts at: 3214 4693 3214 Size: 1479 5893 AclI AA'CG_TT Cuts at: 1563 5499 5872 1563 Size: 3936 373 3063 Fragments arranged by size: 3936 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2117 2378 2486 2790 3656 3794 4079 4118 Size: 485 261 108 304 866 138 285 39 Cuts at: 4118 4441 5435 6819 7087 1632 Size: 323 994 1384 268 1917 Fragments arranged by size: 1917 1384 994 866 485 323 304 285 268 261 138 108 39 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7175 AflIII A'CryG_T Cuts at: 1203 2391 6271 7248 1203 Size: 1188 3880 977 1327 Fragments arranged by size: 3880 1327 1188 977 AgeI A'CCGG_T Cuts at: 6203 6203 Size: 7372 AhdI GACnn_n'nnGTC Cuts at: 4648 6101 4648 Size: 1453 5919 AleI CACnn'nnGTG Cuts at: 1166 2964 1166 Size: 1798 5574 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7372 AlwNI CAG_nnn'CTG Cuts at: 2170 2173 2449 2531 2689 2722 2878 3226 5115 Size: 3 276 82 158 33 156 348 1889 Cuts at: 5115 6687 2170 Size: 1572 2855 Fragments arranged by size: 2855 1889 1572 348 276 158 156 82 33 3 ApaLI G'TGCA_C Cuts at: 5429 6585 5429 Size: 1156 6216 ApoI r'AATT_y Cuts at: 1493 4681 4907 5059 7168 1493 Size: 3188 226 152 2109 1697 Fragments arranged by size: 3188 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5926 160 Size: 872 4894 1606 Fragments arranged by size: 4894 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7372 AvaI C'yCGr_G Cuts at: 1870 1943 2766 3406 3974 4187 4409 4697 1870 Size: 73 823 640 568 213 222 288 4545 Fragments arranged by size: 4545 823 640 568 288 222 213 73 AvrII C'CTAG_G Cuts at: 3614 3614 Size: 7372 BamHI G'GATC_C Cuts at: 3831 4702 5069 3831 Size: 871 367 6134 Fragments arranged by size: 6134 871 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2017 2319 2405 2591 2785 Size: 325 217 627 230 302 86 186 194 Cuts at: 2785 3214 3369 3858 4166 4693 6148 618 Size: 429 155 489 308 527 1455 1842 Fragments arranged by size: 1842 1455 627 527 489 429 325 308 302 230 217 194 186 155 86 BanII G_rGCy'C Cuts at: 1391 2317 3100 3981 4691 1391 Size: 926 783 881 710 4072 Fragments arranged by size: 4072 926 881 783 710 BbeI G_GCGC'C Cuts at: 2021 2595 3373 2021 Size: 574 778 6020 Fragments arranged by size: 6020 778 574 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3509 3961 928 Size: 971 1610 452 4339 Fragments arranged by size: 4339 1610 971 452 BbvCI CC'TCA_GC Cuts at: 2586 2652 3206 2586 Size: 66 554 6752 Fragments arranged by size: 6752 554 66 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5563 5597 1903 Size: 34 3626 34 3678 Fragments arranged by size: 3678 3626 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2786 5264 6474 2786 Size: 2478 1210 3684 Fragments arranged by size: 3684 2478 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7372 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2154 2862 2915 Size: 107 122 71 631 605 482 708 53 Cuts at: 2915 5983 136 Size: 3068 1525 Fragments arranged by size: 3068 1525 708 631 605 482 122 107 71 53 BglII A'GATC_T Cuts at: 7367 7367 Size: 7372 BlpI GC'TnA_GC Cuts at: 2268 3688 3791 4789 2268 Size: 1420 103 998 4851 Fragments arranged by size: 4851 1420 998 103 Bme1580I G_kGCm'C Cuts at: 1790 3861 4277 4397 5433 6589 1790 Size: 2071 416 120 1036 1156 2573 Fragments arranged by size: 2573 2071 1156 1036 416 120 BmgBI CAC'GTC Cuts at: 1318 3626 1318 Size: 2308 5064 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3477 5103 6061 Size: 416 53 83 399 24 2060 1626 958 Cuts at: 6061 442 Size: 1753 Fragments arranged by size: 2060 1753 1626 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7372 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2216 2522 2744 3110 3128 3857 3883 4370 Size: 977 306 222 366 18 729 26 487 Cuts at: 4370 6032 1239 Size: 1662 2579 Fragments arranged by size: 2579 1662 977 729 487 366 306 222 26 18 Bpu10I CC'TnA_GC Cuts at: 2309 2532 2586 2652 3206 2309 Size: 223 54 66 554 6475 Fragments arranged by size: 6475 554 223 66 54 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3188 4385 5497 6362 6660 3188 Size: 1197 1112 865 298 3900 Fragments arranged by size: 3900 1197 1112 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6035 882 Size: 617 4536 2219 Fragments arranged by size: 4536 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2394 3997 5163 493 Size: 711 1190 1603 1166 2702 Fragments arranged by size: 2702 1603 1190 1166 711 BsaBI GATnn'nnATC Cuts at: 2489 3953 7366 2489 Size: 1464 3413 2495 Fragments arranged by size: 3413 2495 1464 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2018 2592 3370 4329 Size: 53 83 186 936 485 574 778 959 Cuts at: 4329 5179 5561 6196 275 Size: 850 382 635 1451 Fragments arranged by size: 1451 959 936 850 778 635 574 485 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2334 4146 5805 6203 6477 6624 7035 2334 Size: 1812 1659 398 274 147 411 2671 Fragments arranged by size: 2671 1812 1659 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2679 2709 1178 Size: 30 165 30 1276 30 5841 Fragments arranged by size: 5841 1276 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2561 3593 4373 4448 4595 1186 Size: 838 537 1032 780 75 147 3963 Fragments arranged by size: 3963 1032 838 780 537 147 75 BseYI C'CCAG_C Cuts at: 2263 2439 2664 2799 2902 3039 3697 4237 6575 Size: 176 225 135 103 137 658 540 2338 Cuts at: 6575 7071 2263 Size: 496 2564 Fragments arranged by size: 2564 2338 658 540 496 225 176 137 135 103 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2183 2210 2549 3835 4575 1163 Size: 573 447 27 339 1286 740 3960 Fragments arranged by size: 3960 1286 740 573 447 339 27 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2294 2910 4822 5583 6611 1540 Size: 275 186 293 616 1912 761 1028 2301 Fragments arranged by size: 2301 1912 1028 761 616 293 275 186 BsiHKAI G_wGCw'C Cuts at: 3413 3792 5433 5518 6589 3413 Size: 379 1641 85 1071 4196 Fragments arranged by size: 4196 1641 1071 379 85 BsmI GAATG_Cn' Cuts at: 4883 4976 4883 Size: 93 7279 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2317 3100 3413 3792 3861 3981 4277 Size: 399 527 783 313 379 69 120 296 Cuts at: 4277 4397 4691 5433 5518 6589 1391 Size: 120 294 742 85 1071 2174 Fragments arranged by size: 2174 1071 783 742 527 399 379 313 296 294 120 120 85 69 BspEI T'CCGG_A Cuts at: 2334 7035 2334 Size: 4701 2671 BspHI T'CATG_A Cuts at: 3378 3945 5261 6991 3378 Size: 567 1316 1730 3759 Fragments arranged by size: 3759 1730 1316 567 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2130 2839 3037 3393 4406 4545 4597 4727 Size: 1286 709 198 356 1013 139 52 130 Cuts at: 4727 844 Size: 3489 Fragments arranged by size: 3489 1286 1013 709 356 198 139 130 52 BsrBI CCG'CTC Cuts at: 1394 1823 5259 1394 Size: 429 3436 3507 Fragments arranged by size: 3507 3436 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5867 6041 65 Size: 1183 4619 174 1396 Fragments arranged by size: 4619 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6016 6203 1700 Size: 110 4206 187 2869 Fragments arranged by size: 4206 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7372 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7372 BssSI C'ACGA_G Cuts at: 3959 4031 5432 6444 3959 Size: 72 1401 1012 4887 Fragments arranged by size: 4887 1401 1012 72 BstAPI GCAn_nnn'nTGC Cuts at: 794 2170 2173 2449 2563 3226 3787 4480 794 Size: 1376 3 276 114 663 561 693 3686 Fragments arranged by size: 3686 1376 693 663 561 276 114 3 BstBI TT'CG_AA Cuts at: 6176 6176 Size: 7372 BstEII G'GTnAC_C Cuts at: 1184 3241 3938 1184 Size: 2057 697 4618 Fragments arranged by size: 4618 2057 697 BstXI CCAn_nnnn'nTGG Cuts at: 2602 2602 Size: 7372 BstYI r'GATC_y Cuts at: 1972 3831 3954 4702 5069 5454 5471 6923 7009 Size: 1859 123 748 367 385 17 1452 86 Cuts at: 7009 7021 7367 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1859 1452 748 385 367 346 123 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2281 3329 2281 Size: 1048 6324 BtgI C'CryG_G Cuts at: 513 1065 2004 2021 2294 513 Size: 552 939 17 273 5591 Fragments arranged by size: 5591 939 552 273 17 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6633 Fragments arranged by size: 6633 683 56 BtsI GCAGTG_nn' Cuts at: 2843 3452 4608 4888 5682 5702 2843 Size: 609 1156 280 794 20 4513 Fragments arranged by size: 4513 1156 794 609 280 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7372 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7337 DraI TTT'AAA Cuts at: 4676 4676 Size: 7372 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4316 6379 809 Size: 507 3000 2063 1802 Fragments arranged by size: 3000 2063 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2133 2291 2907 Size: 54 1425 50 275 18 303 158 616 Cuts at: 2907 2916 2968 3051 4819 5708 7337 8 Size: 9 52 83 1768 889 1629 43 Fragments arranged by size: 1768 1629 1425 889 616 303 275 158 83 54 52 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2291 2907 4819 1537 Size: 275 479 616 1912 4090 Fragments arranged by size: 4090 1912 616 479 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3513 4293 5302 1353 Size: 2160 780 1009 3423 Fragments arranged by size: 3423 2160 1009 780 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2063 2558 2843 5959 6333 6479 Size: 1064 138 474 495 285 3116 374 146 Cuts at: 6479 387 Size: 1280 Fragments arranged by size: 3116 1280 1064 495 474 374 285 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2117 2216 2378 2486 2522 2744 2790 Size: 393 485 99 162 108 36 222 46 Cuts at: 2790 3110 3128 3656 3794 3857 3883 4079 4118 Size: 320 18 528 138 63 26 196 39 Cuts at: 4118 4370 4441 5435 6032 6819 7087 1239 Size: 252 71 994 597 787 268 1524 Fragments arranged by size: 1524 994 787 597 528 485 393 320 268 252 222 196 162 138 108 99 71 63 46 39 36 26 18 EcoNI CCTnn'n_nnAGG Cuts at: 3560 3560 Size: 7372 EcoO109I rG'GnC_Cy Cuts at: 2285 2514 2528 3721 4054 2285 Size: 229 14 1193 333 5603 Fragments arranged by size: 5603 1193 333 229 14 EcoRI G'AATT_C Cuts at: 4681 5059 4681 Size: 378 6994 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3526 3526 Size: 7372 FspI TGC'GCA Cuts at: 2753 5878 2753 Size: 3125 4247 HaeII r_GCGC'y Cuts at: 1678 2021 2595 2899 3373 4408 6519 1678 Size: 343 574 304 474 1035 2111 2531 Fragments arranged by size: 2531 2111 1035 574 474 343 304 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3557 3589 4634 4666 6013 Size: 32 135 32 1985 32 1045 32 1347 Cuts at: 6013 6045 6087 6119 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1985 1347 1045 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4321 4716 4962 669 Size: 988 281 2383 395 246 3079 Fragments arranged by size: 3079 2383 988 395 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7372 HpaI GTT'AAC Cuts at: 4962 4962 Size: 7372 KasI G'GCGC_C Cuts at: 2017 2591 3369 2017 Size: 574 778 6020 Fragments arranged by size: 6020 778 574 KpnI G_GTAC'C Cuts at: 3218 4697 3218 Size: 1479 5893 MfeI C'AATT_G Cuts at: 4971 4971 Size: 7372 MluI A'CGCG_T Cuts at: 7248 7248 Size: 7372 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2208 4044 6486 6670 1305 Size: 903 1836 2442 184 2007 Fragments arranged by size: 2442 2007 1836 903 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2135 2918 10 Size: 54 1425 343 303 783 4464 Fragments arranged by size: 4464 1425 783 343 303 54 MslI CAynn'nnrTG Cuts at: 518 1166 2964 3963 5330 5689 5848 518 Size: 648 1798 999 1367 359 159 2042 Fragments arranged by size: 2042 1798 1367 999 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2023 2170 2203 2267 2344 2435 2464 2579 Size: 417 147 33 64 77 91 29 115 Cuts at: 2579 2719 2906 3684 4788 5465 6613 6858 1606 Size: 140 187 778 1104 677 1148 245 2120 Fragments arranged by size: 2120 1148 1104 778 677 417 245 187 147 140 115 91 77 64 33 29 NarI GG'CG_CC Cuts at: 2018 2592 3370 2018 Size: 574 778 6020 Fragments arranged by size: 6020 778 574 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5881 Fragments arranged by size: 5881 939 552 NdeI CA'TA_TG Cuts at: 387 3606 387 Size: 3219 4153 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7372 NotI GC'GGCC_GC Cuts at: 2907 4819 2907 Size: 1912 5460 NspI r_CATG'y Cuts at: 1132 1229 2351 4730 6275 1132 Size: 97 1122 2379 1545 2229 Fragments arranged by size: 2379 2229 1545 1122 97 PasI CC'CwG_GG Cuts at: 1348 2252 4087 4283 1348 Size: 904 1835 196 4437 Fragments arranged by size: 4437 1835 904 196 PciI A'CATG_T Cuts at: 6271 6271 Size: 7372 PflMI CCAn_nnn'nTGG Cuts at: 1108 4470 1108 Size: 3362 4010 PfoI T'CCnGG_A Cuts at: 3177 3834 4632 4662 3177 Size: 657 798 30 5887 Fragments arranged by size: 5887 798 657 30 PmeI GTTT'AAAC Cuts at: 4676 4676 Size: 7372 PmlI CAC'GTG Cuts at: 2394 2394 Size: 7372 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4262 4294 4541 4573 5441 5473 6954 6986 4262 Size: 32 247 32 868 32 1481 32 4648 Fragments arranged by size: 4648 1481 868 247 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2285 2514 3721 4054 2285 Size: 229 1207 333 5603 Fragments arranged by size: 5603 1207 333 229 PshAI GACnn'nnGTC Cuts at: 1613 3077 1613 Size: 1464 5908 PsiI TTA'TAA Cuts at: 4942 4942 Size: 7372 PstI C_TGCA'G Cuts at: 830 2850 3285 3401 4023 4724 830 Size: 2020 435 116 622 701 3478 Fragments arranged by size: 3478 2020 701 622 435 116 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7372 PvuII CAG'CTG Cuts at: 2170 2203 2267 2344 2435 2464 2579 2719 2170 Size: 33 64 77 91 29 115 140 6823 Fragments arranged by size: 6823 140 115 91 77 64 33 29 SacII CC_GC'GG Cuts at: 2024 2024 Size: 7372 SalI G'TCGA_C Cuts at: 1936 4714 1936 Size: 2778 4594 SanDI GG'GwC_CC Cuts at: 2514 2514 Size: 7372 SbfI CC_TGCA'GG Cuts at: 4724 4724 Size: 7372 ScaI AGT'ACT Cuts at: 5620 7332 5620 Size: 1712 5660 SfcI C'TryA_G Cuts at: 826 1045 2552 2669 2846 3281 3397 4019 4720 Size: 219 1507 117 177 435 116 622 701 Cuts at: 4720 5855 6536 6727 826 Size: 1135 681 191 1471 Fragments arranged by size: 1507 1471 1135 701 681 622 435 219 191 177 117 116 SfiI GGCCn_nnn'nGGCC Cuts at: 2915 2915 Size: 7372 SfoI GGC'GCC Cuts at: 2019 2593 3371 2019 Size: 574 778 6020 Fragments arranged by size: 6020 778 574 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7372 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7372 SmaI CCC'GGG Cuts at: 1872 4699 1872 Size: 2827 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3167 4364 5476 6377 6639 820 Size: 197 926 1224 1197 1112 901 262 1553 Fragments arranged by size: 1553 1224 1197 1112 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5163 493 Size: 4670 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7372 SphI G_CATG'C Cuts at: 1132 4730 1132 Size: 3598 3774 SspI AAT'ATT Cuts at: 5 52 5296 5 Size: 47 5244 2081 Fragments arranged by size: 5244 2081 47 StuI AGG'CCT Cuts at: 1517 3464 1517 Size: 1947 5425 StyI C'CwwG_G Cuts at: 513 1065 2004 2412 2709 2949 3510 3614 4811 Size: 552 939 408 297 240 561 104 1197 Cuts at: 4811 513 Size: 3074 Fragments arranged by size: 3074 1197 939 561 552 408 297 240 104 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3503 3550 4229 4256 5399 5416 5569 Size: 1255 1923 47 679 27 1143 17 153 Cuts at: 5569 5754 6199 325 Size: 185 445 1498 Fragments arranged by size: 1923 1498 1255 1143 679 445 185 153 47 27 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3816 5618 7330 Size: 274 80 33 51 1443 1839 1802 1712 Cuts at: 7330 96 Size: 138 Fragments arranged by size: 1839 1802 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2835 2867 3185 3217 3583 3610 3615 3642 7332 Size: 32 318 32 366 27 5 27 3690 Cuts at: 7332 7364 2835 Size: 32 2843 Fragments arranged by size: 3690 2843 366 318 32 32 32 27 27 5 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7372 XbaI T'CTAG_A Cuts at: 4708 4708 Size: 7372 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2191 2992 2191 Size: 801 6571 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7372 XmaI C'CCGG_G Cuts at: 1870 4697 1870 Size: 2827 4545 XmnI GAAnn'nnTTC Cuts at: 5501 5501 Size: 7372 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5180 276 Size: 53 83 186 936 3646 2468 Fragments arranged by size: 3646 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SapI SexAI SrfI SwaI