################################## # NO CUT ENZYMES ( 178 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MflI MluI MluNI MscI MslI MspA1I MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccIII 113 TCCGGA T^CCGGA AlwI 131 GGATC GGATC(4/5) AlwNI 266 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 310 CCTCAGG CC^T[ACGT]AGG AspHI 46 GAGCTC G[AT]GC[AT]^C BanII 46 GAGCTC G[GA]GC[CT]^C BmyI 46 GAGCTC G[AGT]GC[ACT]^C BsaWI 113 TCCGGA [AT]^CCGG[AT] BseAI 113 TCCGGA T^CCGGA BsiHKAI 46 GAGCTC G[AT]GC[AT]^C Bsp1286I 46 GAGCTC G[AGT]GC[ACT]^C BspEI 113 TCCGGA T^CCGGA BspMI 119 ACCTGC ACCTGC(4/8) BsrBI 154 GAGCGG CCGCTC(-3/-3) Bsu36I 310 CCTCAGG CC^T[ACGT]AGG Cac8I 185 GCGTGC GC[ACGT]^[ACGT]GC CfoI 7 GCGC GCG^C Cfr13I 302 GGGCC G^G[ACGT]CC DpnII 132 GATC ^GATC Eco81I 310 CCTCAGG CC^T[ACGT]AGG EcoRI 197 GAATTC G^AATTC EcoT14I 245 CCTTGG C^C[AT][AT]GG HapII 114 CCGG C^CGG HhaI 7 GCGC GCG^C HinP1I 7 GCGC G^CGC HpaII 114 CCGG C^CGG HphI 391 GGTGA GGTGA(8/7) MaeIII 79 GTCAC ^GT[ACGT]AC MboI 132 GATC ^GATC MboII 138 GAAGA GAAGA(8/7) MroI 113 TCCGGA T^CCGGA MspI 114 CCGG C^CGG NdeII 132 GATC ^GATC NlaIII 418 CATG CATG^ SacI 46 GAGCTC GAGCT^C Sau3AI 132 GATC ^GATC Sau96I 302 GGGCC G^G[ACGT]CC ScaI 424 AGTACT AGT^ACT SfaNI 217 GATGC GCATC(5/9) StyI 245 CCTTGG C^C[AT][AT]GG TaqI 75 TCGA T^CGA Tsp45I 79 GTCAC ^GT[GC]AC TthHB8I 75 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AcsI 141 GAATTT [GA]^AATT[CT] 197 GAATTC AfaI 242 GTAC GT^AC 425 GTAC ApoI 141 GAATTT [GA]^AATT[CT] 197 GAATTC BseRI 308 CTCCTC GAGGAG(10/8) 428 CTCCTC BsiYI 359 CCACACTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 382 CCCACTCCAGG BslI 359 CCACACTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 382 CCCACTCCAGG BsmAI 179 GAGAC GTCTC(1/5) 378 GTCTC BsrI 224 ACTGG ACTGG(1/-1) 234 ACTGG DdeI 311 CTCAG C^T[ACGT]AG 364 CTGAG EaeI 157 CGGCCA [CT]^GGCC[GA] 190 CGGCCA HinfI 77 GAGTC G^A[ACGT]TC 376 GAGTC MseI 65 TTAA T^TAA 212 TTAA MwoI 147 GCTAAAAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 274 GCCCTTGCTGC NlaIV 99 GGAACC GG[ACGT]^[ACGT]CC 116 GGAACC PleI 77 GAGTC GAGTC(4/5) 376 GAGTC RsaI 242 GTAC GT^AC 425 GTAC Tru9I 65 TTAA T^TAA 212 TTAA Tsp509I 142 AATT ^AATT 198 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 19 enzymes) # ######################################### AciI AluI BbvI BpmI BsaJI BsmFI BssKI BstF5I BstNI EcoRII Fnu4HI FokI HaeIII ItaI MnlI MvaI ScrFI TseI TspRI