(Circular) (Six-base) MAPSORT of: pFN21AE4059.seq Check: 2540 from: 1 to: 5686 pFN21AE4059 5686 bp With 182 enzymes: SgfI * March 28, 2012 13:13 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2100 2192 2938 2100 Size: 92 746 4848 Fragments arranged by size: 4848 746 92 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3496 278 Size: 53 83 186 936 1960 2468 Fragments arranged by size: 2468 1960 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3029 1937 Size: 1092 4594 Acc65I G'GTAC_C Cuts at: 2119 3007 2119 Size: 888 4798 AclI AA'CG_TT Cuts at: 1563 3813 4186 1563 Size: 2250 373 3063 Fragments arranged by size: 3063 2250 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2102 3749 5133 5401 1632 Size: 470 1647 1384 268 1917 Fragments arranged by size: 1917 1647 1384 470 268 AfeI AGC'GCT Cuts at: 2655 2655 Size: 5686 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5489 AflIII A'CryG_T Cuts at: 1203 2634 4585 5562 1203 Size: 1431 1951 977 1327 Fragments arranged by size: 1951 1431 1327 977 AgeI A'CCGG_T Cuts at: 4517 4517 Size: 5686 AhdI GACnn_n'nnGTC Cuts at: 4415 4415 Size: 5686 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5686 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2213 2817 1184 Size: 1029 604 4053 Fragments arranged by size: 4053 1029 604 AlwNI CAG_nnn'CTG Cuts at: 2743 3429 5001 2743 Size: 686 1572 3428 Fragments arranged by size: 3428 1572 686 ApaI G_GGCC'C Cuts at: 2298 2298 Size: 5686 ApaLI G'TGCA_C Cuts at: 3743 4899 3743 Size: 1156 4530 ApoI r'AATT_y Cuts at: 1493 2995 3221 3373 5482 1493 Size: 1502 226 152 2109 1697 Fragments arranged by size: 2109 1697 1502 226 152 AseI AT'TA_AT Cuts at: 160 1032 4240 160 Size: 872 3208 1606 Fragments arranged by size: 3208 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5686 AvaI C'yCGr_G Cuts at: 1870 1943 3011 1870 Size: 73 1068 4545 Fragments arranged by size: 4545 1068 73 BamHI G'GATC_C Cuts at: 3016 3383 3016 Size: 367 5319 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2119 2287 2578 3007 4462 Size: 325 217 627 332 168 291 429 1455 Cuts at: 4462 618 Size: 1842 Fragments arranged by size: 1842 1455 627 429 332 325 291 217 168 BanII G_rGCy'C Cuts at: 1391 2298 3005 1391 Size: 907 707 4072 Fragments arranged by size: 4072 907 707 BbeI G_GCGC'C Cuts at: 2291 2582 2291 Size: 291 5395 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4715 BbvCI CC'TCA_GC Cuts at: 2574 2574 Size: 5686 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2619 2653 3877 3911 1903 Size: 34 682 34 1224 34 3678 Fragments arranged by size: 3678 1224 682 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3578 4788 3578 Size: 1210 4476 BclI T'GATC_A Cuts at: 1548 2880 1548 Size: 1332 4354 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4297 136 Size: 107 122 71 631 605 2625 1525 Fragments arranged by size: 2625 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5681 5681 Size: 5686 BlpI GC'TnA_GC Cuts at: 3103 3103 Size: 5686 Bme1580I G_kGCm'C Cuts at: 1790 2298 2806 2837 3747 4903 1790 Size: 508 508 31 910 1156 2573 Fragments arranged by size: 2573 1156 910 508 508 31 BmgBI CAC'GTC Cuts at: 1318 2637 1318 Size: 1319 4367 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2363 3417 4375 Size: 416 53 83 399 24 946 1054 958 Cuts at: 4375 442 Size: 1753 Fragments arranged by size: 1753 1054 958 946 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5686 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2096 2564 4346 1239 Size: 857 468 1782 2579 Fragments arranged by size: 2579 1782 857 468 Bpu10I CC'TnA_GC Cuts at: 2574 2849 2574 Size: 275 5411 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2213 2642 3811 4676 4974 2213 Size: 429 1169 865 298 2925 Fragments arranged by size: 2925 1169 865 429 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2519 2594 4349 882 Size: 617 1020 75 1755 2219 Fragments arranged by size: 2219 1755 1020 617 75 BsaAI yAC'GTr Cuts at: 493 1204 3477 493 Size: 711 2273 2702 Fragments arranged by size: 2702 2273 711 BsaBI GATnn'nnATC Cuts at: 5680 5680 Size: 5686 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2288 2579 3493 3875 Size: 53 83 186 936 755 291 914 382 Cuts at: 3875 4510 275 Size: 635 1451 Fragments arranged by size: 1451 936 914 755 635 382 291 186 83 53 BsaWI w'CCGG_w Cuts at: 4119 4517 4791 4938 5349 4119 Size: 398 274 147 411 4456 Fragments arranged by size: 4456 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5461 Fragments arranged by size: 5461 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2084 2318 2561 1186 Size: 898 234 243 4311 Fragments arranged by size: 4311 898 243 234 BseYI C'CCAG_C Cuts at: 2014 2315 2356 2410 2438 2622 4889 5385 2014 Size: 301 41 54 28 184 2267 496 2315 Fragments arranged by size: 2315 2267 496 301 184 54 41 28 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2551 2813 1163 Size: 573 815 262 4036 Fragments arranged by size: 4036 815 573 262 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3136 3897 4925 1540 Size: 275 186 1135 761 1028 2301 Fragments arranged by size: 2301 1135 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3747 3832 4903 3747 Size: 85 1071 4530 Fragments arranged by size: 4530 1071 85 BsmI GAATG_Cn' Cuts at: 3197 3290 3197 Size: 93 5593 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2298 2806 2837 3005 3747 3832 4903 Size: 399 508 508 31 168 742 85 1071 Cuts at: 4903 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 508 508 399 168 85 31 BspEI T'CCGG_A Cuts at: 5349 5349 Size: 5686 BspHI T'CATG_A Cuts at: 3575 5305 3575 Size: 1730 3956 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2100 2192 2415 2724 2910 2938 3041 844 Size: 1256 92 223 309 186 28 103 3489 Fragments arranged by size: 3489 1256 309 223 186 103 92 28 BsrBI CCG'CTC Cuts at: 1394 1823 2047 2781 3573 1394 Size: 429 224 734 792 3507 Fragments arranged by size: 3507 792 734 429 224 BsrDI GCAATG_nn' Cuts at: 65 1248 2667 4181 4355 65 Size: 1183 1419 1514 174 1396 Fragments arranged by size: 1514 1419 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4330 4517 1700 Size: 110 2520 187 2869 Fragments arranged by size: 2869 2520 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5686 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5686 BssSI C'ACGA_G Cuts at: 3746 4758 3746 Size: 1012 4674 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5686 BstBI TT'CG_AA Cuts at: 4490 4490 Size: 5686 BstEII G'GTnAC_C Cuts at: 1184 2500 2707 1184 Size: 1316 207 4163 Fragments arranged by size: 4163 1316 207 BstYI r'GATC_y Cuts at: 1972 2451 2514 3016 3383 3768 3785 5237 5323 Size: 479 63 502 367 385 17 1452 86 Cuts at: 5323 5335 5681 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 502 479 385 367 346 86 63 17 12 Bsu36I CC'TnA_GG Cuts at: 2202 2202 Size: 5686 BtgI C'CryG_G Cuts at: 513 1065 2004 2175 2271 2496 2826 513 Size: 552 939 171 96 225 330 3373 Fragments arranged by size: 3373 939 552 330 225 171 96 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4947 Fragments arranged by size: 4947 683 56 BtsI GCAGTG_nn' Cuts at: 2347 3202 3996 4016 2347 Size: 855 794 20 4017 Fragments arranged by size: 4017 855 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5686 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5651 DraI TTT'AAA Cuts at: 2990 2990 Size: 5686 DraIII CAC_nnn'GTG Cuts at: 2461 2461 Size: 5686 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2639 4693 809 Size: 507 1323 2054 1802 Fragments arranged by size: 2054 1802 1323 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2127 2178 Size: 54 1425 50 275 18 177 120 51 Cuts at: 2178 2478 3133 4022 5651 8 Size: 300 655 889 1629 43 Fragments arranged by size: 1629 1425 889 655 300 275 177 120 54 51 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3133 1537 Size: 275 1321 4090 Fragments arranged by size: 4090 1321 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3616 1353 Size: 2263 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2671 2765 2882 4273 4647 4793 Size: 1064 138 1082 94 117 1391 374 146 Cuts at: 4793 387 Size: 1280 Fragments arranged by size: 1391 1280 1082 1064 374 146 138 117 94 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2096 2102 2564 3749 4346 5133 5401 Size: 393 464 6 462 1185 597 787 268 Cuts at: 5401 1239 Size: 1524 Fragments arranged by size: 1524 1185 787 597 464 462 393 268 6 EcoNI CCTnn'n_nnAGG Cuts at: 2328 2861 2328 Size: 533 5153 EcoO109I rG'GnC_Cy Cuts at: 2294 2321 2866 2294 Size: 27 545 5114 Fragments arranged by size: 5114 545 27 EcoRI G'AATT_C Cuts at: 2995 3373 2995 Size: 378 5308 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2053 2053 Size: 5686 FspI TGC'GCA Cuts at: 4192 4192 Size: 5686 HaeII r_GCGC'y Cuts at: 1678 2291 2582 2657 2815 4833 1678 Size: 613 291 75 158 2018 2531 Fragments arranged by size: 2531 2018 613 291 158 75 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2518 2550 4327 4359 4401 Size: 32 135 32 946 32 1777 32 42 Cuts at: 4401 4433 1373 Size: 32 2626 Fragments arranged by size: 2626 1777 946 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2170 3030 3276 669 Size: 988 281 232 860 246 3079 Fragments arranged by size: 3079 988 860 281 246 232 HindIII A'AGCT_T Cuts at: 748 748 Size: 5686 HpaI GTT'AAC Cuts at: 3276 3276 Size: 5686 KasI G'GCGC_C Cuts at: 2287 2578 2287 Size: 291 5395 KpnI G_GTAC'C Cuts at: 2123 3011 2123 Size: 888 4798 MfeI C'AATT_G Cuts at: 3285 3285 Size: 5686 MluI A'CGCG_T Cuts at: 5562 5562 Size: 5686 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4800 4984 1305 Size: 3495 184 2007 Fragments arranged by size: 3495 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2180 10 Size: 54 1425 343 348 3516 Fragments arranged by size: 3516 1425 348 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3644 4003 4162 518 Size: 648 2478 359 159 2042 Fragments arranged by size: 2478 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2360 2704 3102 3779 4927 5172 1606 Size: 754 344 398 677 1148 245 2120 Fragments arranged by size: 2120 1148 754 677 398 344 245 NarI GG'CG_CC Cuts at: 2288 2579 2288 Size: 291 5395 NcoI C'CATG_G Cuts at: 513 1065 2004 2826 513 Size: 552 939 822 3373 Fragments arranged by size: 3373 939 822 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5686 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5686 NotI GC'GGCC_GC Cuts at: 3133 3133 Size: 5686 NspI r_CATG'y Cuts at: 1132 1229 2630 2723 3044 4589 1132 Size: 97 1401 93 321 1545 2229 Fragments arranged by size: 2229 1545 1401 321 97 93 PasI CC'CwG_GG Cuts at: 1348 2942 1348 Size: 1594 4092 PciI A'CATG_T Cuts at: 4585 4585 Size: 5686 PflMI CCAn_nnn'nTGG Cuts at: 1108 2461 2827 1108 Size: 1353 366 3967 Fragments arranged by size: 3967 1353 366 PmeI GTTT'AAAC Cuts at: 2990 2990 Size: 5686 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3755 3787 5268 5300 3755 Size: 32 1481 32 4141 Fragments arranged by size: 4141 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2866 2866 Size: 5686 PshAI GACnn'nnGTC Cuts at: 1613 2237 1613 Size: 624 5062 PsiI TTA'TAA Cuts at: 3256 3256 Size: 5686 PspOMI G'GGCC_C Cuts at: 2294 2294 Size: 5686 PstI C_TGCA'G Cuts at: 830 2769 2907 3038 830 Size: 1939 138 131 3478 Fragments arranged by size: 3478 1939 138 131 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5686 PvuII CAG'CTG Cuts at: 2360 2360 Size: 5686 SalI G'TCGA_C Cuts at: 1936 3028 1936 Size: 1092 4594 SbfI CC_TGCA'GG Cuts at: 3038 3038 Size: 5686 ScaI AGT'ACT Cuts at: 2564 3934 5646 2564 Size: 1370 1712 2604 Fragments arranged by size: 2604 1712 1370 SfcI C'TryA_G Cuts at: 826 1045 2361 2765 2903 3034 4169 4850 5041 Size: 219 1316 404 138 131 1135 681 191 Cuts at: 5041 826 Size: 1471 Fragments arranged by size: 1471 1316 1135 681 404 219 191 138 131 SfoI GGC'GCC Cuts at: 2289 2580 2289 Size: 291 5395 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5686 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5686 SmaI CCC'GGG Cuts at: 1872 3013 1872 Size: 1141 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2228 2657 3790 4691 4953 820 Size: 197 926 285 429 1133 901 262 1553 Fragments arranged by size: 1553 1133 926 901 429 285 262 197 SnaBI TAC'GTA Cuts at: 493 3477 493 Size: 2984 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5686 SphI G_CATG'C Cuts at: 1132 2630 3044 1132 Size: 1498 414 3774 Fragments arranged by size: 3774 1498 414 SspI AAT'ATT Cuts at: 5 52 3610 5 Size: 47 3558 2081 Fragments arranged by size: 3558 2081 47 StuI AGG'CCT Cuts at: 1517 2798 1517 Size: 1281 4405 StyI C'CwwG_G Cuts at: 513 1065 2004 2826 3125 513 Size: 552 939 822 299 3074 Fragments arranged by size: 3074 939 822 552 299 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2249 2629 3713 3730 3883 4068 4513 Size: 1255 669 380 1084 17 153 185 445 Cuts at: 4513 325 Size: 1498 Fragments arranged by size: 1498 1255 1084 669 445 380 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2562 3932 5644 Size: 274 80 33 51 1443 585 1370 1712 Cuts at: 5644 96 Size: 138 Fragments arranged by size: 1712 1443 1370 585 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2320 2352 5646 5678 2320 Size: 32 3294 32 2328 Fragments arranged by size: 3294 2328 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5686 XbaI T'CTAG_A Cuts at: 3022 3022 Size: 5686 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5686 XmaI C'CCGG_G Cuts at: 1870 3011 1870 Size: 1141 4545 XmnI GAAnn'nnTTC Cuts at: 2105 3815 2105 Size: 1710 3976 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3494 276 Size: 53 83 186 936 1960 2468 Fragments arranged by size: 2468 1960 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI