################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## AatII AccIII AclI AgeI AhdI Aor51HI ApaI AscI AspEI AspI AviII BbeI BlpI Bpu1102I BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspLU11I BsrFI BssHII Bst1107I BstAPI BstBI BstZ17I CelII Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI FseI FspI HincII HindII HpaI KasI KspI MamI MluI MroI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PaeR7I PflFI PinAI PmeI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 70 enzymes) # ################################### Acc65I 439 GGTACC G^GTACC AccI 98 GTAGAC GT^[AC][GT]AC AccII 1307 CGCG CG^CG AcyI 1525 GGCGTC G[GA]^CG[CT]C AflII 498 CTTAAG C^TTAAG AflIII 399 ACGTGT A^C[GA][CT]GT Alw44I 259 GTGCAC G^TGCAC AlwNI 2405 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 2917 CCTGAGG CC^T[ACGT]AGG ApaLI 259 GTGCAC G^TGCAC Asp700I 541 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 439 GGTACC G^GTACC AvaI 2484 CCCGGG C^[CT]CG[GA]G AvrII 90 CCTAGG C^CTAGG BalI 29 TGGCCA TGG^CCA BamHI 2870 GGATCC G^GATCC BanI 439 GGTACC G^G[CT][GA]CC BbrPI 2077 CACGTG CAC^GTG BbvCI 1800 GCTGAGG CCTCAGC(-5/-2) BcgI 2579 GCAGAAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1838 TGATCA T^GATCA BfrI 498 CTTAAG C^TTAAG BlnI 90 CCTAGG C^CTAGG BsaAI 2077 CACGTG [CT]AC^GT[GA] BsaHI 1525 GGCGTC G[GA]^CG[CT]C BsmI 219 GAATGC GAATGC(1/-1) BsoBI 2484 CCCGGG C^[CT]CG[GA]G Bsp1407I 2549 TGTACA T^GTACA BspHI 1467 TCATGA T^CATGA BsrBI 966 CCGCTC CCGCTC(-3/-3) BsrDI 665 GCAATG GCAATG(2/0) BsrGI 2549 TGTACA T^GTACA BssSI 2572 CTCGTG CACGAG(-5/-1) BstEII 1869 GGTCACC G^GT[ACGT]ACC BstPI 1869 GGTCACC G^GT[ACGT]ACC BstUI 1307 CGCG CG^CG Bsu36I 2917 CCTGAGG CC^T[ACGT]AGG DraI 699 TTTAAA TTT^AAA DraIII 2074 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 29 TGGCCA [CT]^GGCC[GA] Eco81I 2917 CCTGAGG CC^T[ACGT]AGG EcoO65I 1869 GGTCACC G^GT[ACGT]ACC EcoRV 901 GATATC GAT^ATC EcoT22I 1594 ATGCAT ATGCA^T FbaI 1838 TGATCA T^GATCA HaeII 2778 AGCGCC [GA]GCGC^[CT] Hin1I 1525 GGCGTC G[GA]^CG[CT]C HindIII 1398 AAGCTT A^AGCTT KpnI 439 GGTACC GGTAC^C MfeI 2264 CAATTG C^AATTG MluNI 29 TGGCCA TGG^CCA MscI 29 TGGCCA TGG^CCA MunI 2264 CAATTG C^AATTG MvnI 1307 CGCG CG^CG NsiI 1594 ATGCAT ATGCA^T PacI 1135 TTAATTAA TTAAT^TAA PflMI 1057 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 2077 CACGTG CAC^GTG PmlI 2077 CACGTG CAC^GTG PshAI 2762 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1467 TCATGA T^CATGA SexAI 2339 ACCTGGT A^CC[AT]GGT SmaI 2484 CCCGGG CCC^GGG SspBI 2549 TGTACA T^GTACA StuI 1499 AGGCCT AGG^CCT Van91I 1057 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 2300 CCACCGACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 2484 CCCGGG C^CCGGG XmaI 2484 CCCGGG C^CCGGG XmnI 541 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AseI 1134 ATTAAT AT^TAAT 1138 ATTAAT AsnI 1134 ATTAAT AT^TAAT 1138 ATTAAT BciVI 759 GGATAC GTATCC(6/5) 782 GGATAC BglI 1324 GCCCGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2822 GCCAAGGAGGC BglII 1213 AGATCT A^GATCT 1937 AGATCT BseRI 2471 CTCCTC GAGGAG(10/8) 1318 GAGGAG BsgI 2071 CTGCAC GTGCAG(16/14) 1481 GTGCAG BspMI 870 GCAGGT ACCTGC(4/8) 1718 GCAGGT CfoI 1306 GCGC GCG^C 2779 GCGC EcoRI 1108 GAATTC G^AATTC 1918 GAATTC HgaI 1526 GCGTC GACGC(5/10) 2224 GCGTC HhaI 1306 GCGC GCG^C 2779 GCGC HinP1I 1306 GCGC G^CGC 2779 GCGC NcoI 1958 CCATGG C^CATGG 2309 CCATGG NspI 1253 ACATGC [GA]CATG^[CT] 1596 GCATGT PpuMI 2722 AGGACCC [GA]G^G[AT]CC[CT] 2945 AGGACCC PshBI 1134 ATTAAT AT^TAAT 1138 ATTAAT SspI 302 AATATT AAT^ATT 1035 AATATT TaqI 2022 TCGA T^CGA 2588 TCGA TthHB8I 2022 TCGA T^CGA 2588 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII