Restriction map for
pFN21AE4253
(Linear) (Six-base) MAPSORT of: KIEE4253.seq Check: 512 from: 1 to: 3039
KIEE4253.orf
With 182 enzymes: SgfI *
March 22, 2012 17:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 861 3039
Size: 861 2178
AccI GT'mk_AC
Cuts at: 0 99 3039
Size: 99 2940
Acc65I G'GTAC_C
Cuts at: 0 439 3039
Size: 439 2600
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 1394 1650 2009 2049 2620 3039
Size: 194 1200 256 359 40 571 419
Fragments arranged by size:
1200 571 419 359 256 194 40
AflII C'TTAA_G
Cuts at: 0 498 3039
Size: 498 2541
AflIII A'CryG_T
Cuts at: 0 399 3039
Size: 399 2640
AleI CACnn'nnGTG
Cuts at: 0 913 3039
Size: 913 2126
AlwNI CAG_nnn'CTG
Cuts at: 0 2410 3039
Size: 2410 629
ApaLI G'TGCA_C
Cuts at: 0 259 3039
Size: 259 2780
ApoI r'AATT_y
Cuts at: 0 520 542 603 694 1108 1918 2466 2531
Size: 520 22 61 91 414 810 548 65
Cuts at: 2531 3039
Size: 508
Fragments arranged by size:
810 548 520 508 414 91 65 61
22
AseI AT'TA_AT
Cuts at: 0 1135 1139 3039
Size: 1135 4 1900
Fragments arranged by size:
1900 1135 4
AvaI C'yCGr_G
Cuts at: 0 2484 3039
Size: 2484 555
AvrII C'CTAG_G
Cuts at: 0 90 3039
Size: 90 2949
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2486 2519 3039
Size: 2486 33 520
Fragments arranged by size:
2486 520 33
BamHI G'GATC_C
Cuts at: 0 2870 3039
Size: 2870 169
BanI G'GyrC_C
Cuts at: 0 439 3039
Size: 439 2600
BanII G_rGCy'C
Cuts at: 0 358 598 1442 3039
Size: 358 240 844 1597
Fragments arranged by size:
1597 844 358 240
BbsI GAAGACnn'nnnn_
Cuts at: 0 92 1656 3026 3039
Size: 92 1564 1370 13
Fragments arranged by size:
1564 1370 92 13
BbvCI CC'TCA_GC
Cuts at: 0 1801 3039
Size: 1801 1238
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2568 2602 3039
Size: 2568 34 437
Fragments arranged by size:
2568 437 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 753 776 3039
Size: 753 23 2263
Fragments arranged by size:
2263 753 23
BclI T'GATC_A
Cuts at: 0 1838 3039
Size: 1838 1201
BfrBI ATG'CAT
Cuts at: 0 1596 3039
Size: 1596 1443
BglI GCCn_nnn'nGGC
Cuts at: 0 1330 2828 3039
Size: 1330 1498 211
Fragments arranged by size:
1498 1330 211
BglII A'GATC_T
Cuts at: 0 1213 1937 3039
Size: 1213 724 1102
Fragments arranged by size:
1213 1102 724
Bme1580I G_kGCm'C
Cuts at: 0 263 1562 2463 2927 3039
Size: 263 1299 901 464 112
Fragments arranged by size:
1299 901 464 263 112
BmgBI CAC'GTC
Cuts at: 0 103 258 3039
Size: 103 155 2781
Fragments arranged by size:
2781 155 103
BmrI ACTGGGnnnn_n'
Cuts at: 0 1437 2749 3039
Size: 1437 1312 290
Fragments arranged by size:
1437 1312 290
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2943 3039
Size: 2943 96
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2367 2456 2483 3039
Size: 2367 89 27 556
Fragments arranged by size:
2367 556 89 27
Bpu10I CC'TnA_GC
Cuts at: 0 1801 3039
Size: 1801 1238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1624 1714 3039
Size: 1624 90 1325
Fragments arranged by size:
1624 1325 90
BsaI GGTCTCn'nnnn_
Cuts at: 0 1600 2433 2982 3039
Size: 1600 833 549 57
Fragments arranged by size:
1600 833 549 57
BsaAI yAC'GTr
Cuts at: 0 2079 3039
Size: 2079 960
BsaHI Gr'CG_yC
Cuts at: 0 1526 3039
Size: 1526 1513
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1429 1459 3039
Size: 1429 30 1580
Fragments arranged by size:
1580 1429 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1333 2462 3039
Size: 1333 1129 577
Fragments arranged by size:
1333 1129 577
BseYI C'CCAG_C
Cuts at: 0 1708 3039
Size: 1708 1331
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1502 2056 3039
Size: 1502 554 983
Fragments arranged by size:
1502 983 554
BsiHKAI G_wGCw'C
Cuts at: 0 263 1680 2053 3039
Size: 263 1417 373 986
Fragments arranged by size:
1417 986 373 263
BsmI GAATG_Cn'
Cuts at: 0 225 3039
Size: 225 2814
Bsp1286I G_dGCh'C
Cuts at: 0 263 358 598 1442 1562 1680 2053 2463
Size: 263 95 240 844 120 118 373 410
Cuts at: 2463 2927 3039
Size: 464 112
Fragments arranged by size:
844 464 410 373 263 240 120 118
112 95
BspHI T'CATG_A
Cuts at: 0 1467 3039
Size: 1467 1572
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 861 1709 3039
Size: 861 848 1330
Fragments arranged by size:
1330 861 848
BsrBI CCG'CTC
Cuts at: 0 968 3039
Size: 968 2071
BsrDI GCAATG_nn'
Cuts at: 0 672 3039
Size: 672 2367
BsrGI T'GTAC_A
Cuts at: 0 2549 3039
Size: 2549 490
BssSI C'ACGA_G
Cuts at: 0 2572 3039
Size: 2572 467
BstEII G'GTnAC_C
Cuts at: 0 1869 3039
Size: 1869 1170
BstXI CCAn_nnnn'nTGG
Cuts at: 0 181 513 2649 3039
Size: 181 332 2136 390
Fragments arranged by size:
2136 390 332 181
BstYI r'GATC_y
Cuts at: 0 130 1213 1415 1485 1937 2315 2870 3039
Size: 130 1083 202 70 452 378 555 169
Fragments arranged by size:
1083 555 452 378 202 169 130 70
Bsu36I CC'TnA_GG
Cuts at: 0 2918 3039
Size: 2918 121
BtgI C'CryG_G
Cuts at: 0 747 1432 1958 2309 3039
Size: 747 685 526 351 730
Fragments arranged by size:
747 730 685 526 351
BtsI GCAGTG_nn'
Cuts at: 0 2348 2852 2868 3039
Size: 2348 504 16 171
Fragments arranged by size:
2348 504 171 16
DraI TTT'AAA
Cuts at: 0 701 3039
Size: 701 2338
DraIII CAC_nnn'GTG
Cuts at: 0 2079 3039
Size: 2079 960
EaeI y'GGCC_r
Cuts at: 0 29 3039
Size: 29 3010
EarI CTCTTCn'nnn_
Cuts at: 0 213 346 1040 1708 1985 3039
Size: 213 133 694 668 277 1054
Fragments arranged by size:
1054 694 668 277 213 133
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1348 2308 3039
Size: 1348 960 731
Fragments arranged by size:
1348 960 731
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 1394 1650 2009 2049 2367 2456 2483
Size: 194 1200 256 359 40 318 89 27
Cuts at: 2483 2620 3039
Size: 137 419
Fragments arranged by size:
1200 419 359 318 256 194 137 89
40 27
EcoO109I rG'GnC_Cy
Cuts at: 0 532 1689 2723 2830 2946 3039
Size: 532 1157 1034 107 116 93
Fragments arranged by size:
1157 1034 532 116 107 93
EcoRI G'AATT_C
Cuts at: 0 1108 1918 3039
Size: 1108 810 1121
Fragments arranged by size:
1121 1108 810
EcoRV GAT'ATC
Cuts at: 0 903 3039
Size: 903 2136
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 513 701 2625 3039
Size: 513 188 1924 414
Fragments arranged by size:
1924 513 414 188
HaeII r_GCGC'y
Cuts at: 0 2782 3039
Size: 2782 257
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 990 1022 1378 1410 1537 1569 1986 2018
Size: 990 32 356 32 127 32 417 32
Cuts at: 2018 2416 2448 3039
Size: 398 32 591
Fragments arranged by size:
990 591 417 398 356 127 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 1398 3039
Size: 1398 1641
KpnI G_GTAC'C
Cuts at: 0 443 3039
Size: 443 2596
MfeI C'AATT_G
Cuts at: 0 2264 3039
Size: 2264 775
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1724 2224 2369 3039
Size: 1724 500 145 670
Fragments arranged by size:
1724 670 500 145
MscI TGG'CCA
Cuts at: 0 31 3039
Size: 31 3008
MslI CAynn'nnrTG
Cuts at: 0 913 1769 1963 2011 2647 3039
Size: 913 856 194 48 636 392
Fragments arranged by size:
913 856 636 392 194 48
MspA1I CmG'CkG
Cuts at: 0 1987 2367 2373 2416 3039
Size: 1987 380 6 43 623
Fragments arranged by size:
1987 623 380 43 6
NcoI C'CATG_G
Cuts at: 0 1958 2309 3039
Size: 1958 351 730
Fragments arranged by size:
1958 730 351
NsiI A_TGCA'T
Cuts at: 0 1598 3039
Size: 1598 1441
NspI r_CATG'y
Cuts at: 0 1257 1600 3039
Size: 1257 343 1439
Fragments arranged by size:
1439 1257 343
PacI TTA_AT'TAA
Cuts at: 0 1139 3039
Size: 1139 1900
PasI CC'CwG_GG
Cuts at: 0 536 1490 2517 3039
Size: 536 954 1027 522
Fragments arranged by size:
1027 954 536 522
PflMI CCAn_nnn'nTGG
Cuts at: 0 1063 3039
Size: 1063 1976
PmlI CAC'GTG
Cuts at: 0 2079 3039
Size: 2079 960
PpuMI rG'GwC_Cy
Cuts at: 0 2723 2946 3039
Size: 2723 223 93
Fragments arranged by size:
2723 223 93
PshAI GACnn'nnGTC
Cuts at: 0 2766 3039
Size: 2766 273
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 262 294 3039
Size: 262 32 2745
Fragments arranged by size:
2745 262 32
PstI C_TGCA'G
Cuts at: 0 1456 1732 2355 2372 2415 3039
Size: 1456 276 623 17 43 624
Fragments arranged by size:
1456 624 623 276 43 17
PvuII CAG'CTG
Cuts at: 0 1987 2367 2373 2416 3039
Size: 1987 380 6 43 623
Fragments arranged by size:
1987 623 380 43 6
SapI GCTCTTCn'nnn_
Cuts at: 0 213 346 1708 3039
Size: 213 133 1362 1331
Fragments arranged by size:
1362 1331 213 133
SexAI A'CCwGG_T
Cuts at: 0 2339 3039
Size: 2339 700
SfcI C'TryA_G
Cuts at: 0 96 462 1452 1728 2160 2351 2368 2411
Size: 96 366 990 276 432 191 17 43
Cuts at: 2411 3039
Size: 628
Fragments arranged by size:
990 628 432 366 276 191 96 43
17
SmaI CCC'GGG
Cuts at: 0 2486 3039
Size: 2486 553
SmlI C'TyrA_G
Cuts at: 0 498 1603 1693 3039
Size: 498 1105 90 1346
Fragments arranged by size:
1346 1105 498 90
SspI AAT'ATT
Cuts at: 0 304 1037 3039
Size: 304 733 2002
Fragments arranged by size:
2002 733 304
StuI AGG'CCT
Cuts at: 0 1501 3039
Size: 1501 1538
StyI C'CwwG_G
Cuts at: 0 90 807 1958 2309 2447 2618 2823 2885
Size: 90 717 1151 351 138 171 205 62
Cuts at: 2885 3039
Size: 154
Fragments arranged by size:
1151 717 351 205 171 154 138 90
62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 700 1311 2649 3039
Size: 700 611 1338 390
Fragments arranged by size:
1338 700 611 390
TatI w'GTAC_w
Cuts at: 0 1925 2549 3039
Size: 1925 624 490
Fragments arranged by size:
1925 624 490
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1429 1461 2668 2700 3039
Size: 1429 32 1207 32 339
Fragments arranged by size:
1429 1207 339 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2307 3039
Size: 2307 732
XmaI C'CCGG_G
Cuts at: 0 2484 3039
Size: 2484 555
XmnI GAAnn'nnTTC
Cuts at: 0 545 3039
Size: 545 2494
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AflIII AleI AlwNI
ApaLI ApoI AseI AvaI AvrII BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrGI BssSI
BstEII BstXI BstYI Bsu36I BtgI BtsI DraI DraIII
EaeI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HindIII KpnI MfeI MmeI MscI MslI
MspA1I NcoI NsiI NspI PacI PasI PflMI PmlI
PpuMI PshAI PsrI PstI PvuII SapI SexAI SfcI
SmaI SmlI SspI StuI StyI TaqII TatI TstI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AgeI AhdI AlfI AloI ApaI
AscI AsiSI BbeI BlpI BmtI BsaBI BsaWI BsiEI
BsiWI BsmBI BspEI BsrFI BssHII BstAPI BstBI BstZ17I
BtgZI ClaI CspCI DrdI EagI EcoICRI EcoNI FseI
FspI FspAI HincII HpaI KasI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI PciI PfoI PmeI
PpiI PsiI PspOMI PspXI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SwaI Tth111I XbaI XhoI
ZraI