################################## # NO CUT ENZYMES ( 157 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI BspDI BspEI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV FauI FbaI FseI FspI HapII HgaI Hin1I HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MflI MluI MroI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI TaqI TfiI Tth111I TthHB8I Van91I XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AciI 357 CCGC CCGC(-3/-1) AflIII 470 ACACGT A^C[GA][CT]GT AlwNI 812 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 890 GGGCCC GGGCC^C BanI 604 GGCACC G^G[CT][GA]CC BanII 890 GGGCCC G[GA]GC[CT]^C BfaI 322 CTAG C^TAG BpmI 53 CTCCAG CTGGAG(16/14) BsgI 151 CTGCAC GTGCAG(16/14) Bsp1407I 824 TGTACA T^GTACA BspHI 398 TCATGA T^CATGA BsrGI 824 TGTACA T^GTACA BstAPI 811 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 230 CCAAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 49 GCGC GCG^C DpnII 319 GATC ^GATC DraIII 652 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoT14I 344 CCATGG C^C[AT][AT]GG EcoT22I 630 ATGCAT ATGCA^T HaeII 48 AGCGCC [GA]GCGC^[CT] HhaI 49 GCGC GCG^C HinP1I 49 GCGC G^CGC HincII 199 GTTGAC GT[CT]^[GA]AC HindII 199 GTTGAC GT[CT]^[GA]AC MaeI 322 CTAG C^TAG MboI 319 GATC ^GATC MseI 211 TTAA T^TAA MspA1I 815 CAGCTG C[AC]G^C[GT]G NcoI 344 CCATGG C^CATGG NdeII 319 GATC ^GATC NsiI 630 ATGCAT ATGCA^T PstI 714 CTGCAG CTGCA^G PvuII 815 CAGCTG CAG^CTG RcaI 398 TCATGA T^CATGA SapI 903 GCTCTTC GCTCTTC(1/4) Sau3AI 319 GATC ^GATC SspBI 824 TGTACA T^GTACA StuI 701 AGGCCT AGG^CCT StyI 344 CCATGG C^C[AT][AT]GG Tru9I 211 TTAA T^TAA TspRI 762 CAGTG CA[GC]TG[ACGT][ACGT]^ XbaI 321 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 25 GAATTT [GA]^AATT[CT] 80 GAATTT AocI 669 CCTGAGG CC^T[ACGT]AGG 867 CCTAAGG ApoI 25 GAATTT [GA]^AATT[CT] 80 GAATTT BmyI 416 GTGCCC G[AGT]GC[ACT]^C 890 GGGCCC Bsp1286I 416 GTGCCC G[AGT]GC[ACT]^C 890 GGGCCC BsrI 112 ACTGG ACTGG(1/-1) 505 ACTGG BssKI 132 CCTGG ^CC[ACGT]GG 336 CCTGG BstF5I 129 CATCC GGATG(2/0) 648 CATCC BstNI 132 CCTGG CC^[AT]GG 336 CCTGG Bsu36I 669 CCTGAGG CC^T[ACGT]AGG 867 CCTAAGG Cac8I 426 GCTTGC GC[ACGT]^[ACGT]GC 660 GCCAGC DdeI 670 CTGAG C^T[ACGT]AG 868 CTAAG DsaI 344 CCATGG C^C[GA][CT]GG 684 CCGTGG Eco81I 669 CCTGAGG CC^T[ACGT]AGG 867 CCTAAGG EcoRII 132 CCTGG ^CC[AT]GG 336 CCTGG FokI 129 CATCC GGATG(9/13) 648 CATCC MaeII 214 ACGT A^CGT 472 ACGT MvaI 132 CCTGG CC^[AT]GG 336 CCTGG PpuMI 440 GGGTCCT [GA]G^G[AT]CC[CT] 751 GGGACCC ScrFI 132 CCTGG CC^[ACGT]GG 336 CCTGG SfaNI 234 GATGC GCATC(5/9) 258 GCATC SfcI 714 CTGCAG C^T[GA][CT]AG 861 CTATAG ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AfaI AluI AvaII BalI BbvI BsaJI BsiYI BslI BsmFI BspMI BsrDI Cfr13I DraII EaeI EarI EcoO109I Fnu4HI HaeIII HinfI HphI ItaI Ksp632I MaeIII MboII MluNI MnlI MscI MslI MwoI NlaIII NlaIV PleI RsaI Sau96I TseI Tsp45I Tsp509I