################################## # NO CUT ENZYMES ( 171 enzymes) # ################################## AatII AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI Asp700I AspEI AspI AvaI AviII AvrII BanII BbeI BbrPI BbvCI BcgI BclI BcnI BfaI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeII HapII HhaI Hin1I HinP1I HincII HindII HpaI HpaII KasI KspI MaeI MaeII MamI MfeI MluI MroI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI TaqI Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### Acc65I 537 GGTACC G^GTACC AseI 220 ATTAAT AT^TAAT AsnI 220 ATTAAT AT^TAAT Asp718I 537 GGTACC G^GTACC AspHI 562 GAGCAC G[AT]GC[AT]^C BamHI 340 GGATCC G^GATCC BanI 537 GGTACC G^G[CT][GA]CC BciVI 669 GGATAC GTATCC(6/5) BglII 404 AGATCT A^GATCT BmyI 562 GAGCAC G[AGT]GC[ACT]^C BsiHKAI 562 GAGCAC G[AT]GC[AT]^C BsiYI 510 CCCAGAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 510 CCCAGAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 676 GTCTC GTCTC(1/5) Bsp1286I 562 GAGCAC G[AGT]GC[ACT]^C Bsp1407I 460 TGTACA T^GTACA BsrGI 460 TGTACA T^GTACA BssKI 337 CCAGG ^CC[ACGT]GG BstNI 337 CCAGG CC^[AT]GG DdeI 678 CTCAG C^T[ACGT]AG DraI 387 TTTAAA TTT^AAA DraII 328 AGGACCT [GA]G^G[ACGT]CC[CT] EarI 256 GAAGAG CTCTTC(1/4) EcoO109I 328 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRII 337 CCAGG ^CC[AT]GG HgaI 525 GACGC GACGC(5/10) HindIII 379 AAGCTT A^AGCTT KpnI 537 GGTACC GGTAC^C Ksp632I 256 GAAGAG CTCTTC(1/4) MvaI 337 CCAGG CC^[AT]GG PpuMI 328 AGGACCT [GA]G^G[AT]CC[CT] PshBI 220 ATTAAT AT^TAAT PstI 467 CTGCAG CTGCA^G ScrFI 337 CCAGG CC^[ACGT]GG SfaNI 753 GCATC GCATC(5/9) SfcI 467 CTGCAG C^T[GA][CT]AG SmlI 295 CTCAAG C^T[CT][GA]AG SspBI 460 TGTACA T^GTACA SspI 217 AATATT AAT^ATT TfiI 501 GAATC G^A[AT]TC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AciI 24 CCGC CCGC(-3/-1) 156 CCGC AlwI 341 GATCC GGATC(4/5) 340 GGATC AvaII 30 GGTCC G^G[AT]CC 329 GGACC BbsI 93 GAAGAC GAAGAC(2/6) 765 GAAGAC BpuAI 93 GAAGAC GAAGAC(2/6) 765 GAAGAC BsmFI 523 GGGAC GGGAC(10/14) 201 GTCCC BstYI 340 GGATCC [GA]^GATC[CT] 404 AGATCT Cac8I 381 GCTTGC GC[ACGT]^[ACGT]GC 445 GCCAGC Cfr13I 30 GGTCC G^G[ACGT]CC 329 GGACC MflI 340 GGATCC [GA]^GATC[CT] 404 AGATCT MwoI 136 GCCTATTCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 472 GCTATCAAGGC NlaIV 340 GGATCC GG[ACGT]^[ACGT]CC 537 GGTACC PleI 315 GACTC GAGTC(4/5) 616 GACTC Sau96I 30 GGTCC G^G[ACGT]CC 329 GGACC XhoII 340 GGATCC [GA]^GATC[CT] 404 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AfaI AluI BalI BbvI BsaJI BsrI DpnII EaeI EcoT14I Fnu4HI HaeIII HinfI HphI ItaI MaeIII MboI MboII MluNI MnlI MscI MseI MspA1I NdeII NlaIII PvuII RsaI Sau3AI StyI Tru9I TseI Tsp509I TspRI