(Linear) (Six-base) MAPSORT of: KIEE3092.seq  Check: 5024  from: 1  to: 786



KIEE3092.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:13  ..

Acc65I G'GTAC_C

Cuts at:      0     537     786 
   Size:        537     249
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     676     786 
   Size:        112     564     110

  Fragments arranged by size: 

                 564     112     110
 
AseI AT'TA_AT

Cuts at:      0     221     786 
   Size:        221     565
 
BamHI G'GATC_C

Cuts at:      0     340     786 
   Size:        340     446
 
BanI G'GyrC_C

Cuts at:      0     537     786 
   Size:        537     249
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     100     772     786 
   Size:        100     672      14

  Fragments arranged by size: 

                 672     100      14
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     663     786 
   Size:        663     123
 
BglII A'GATC_T

Cuts at:      0     404     786 
   Size:        404     382
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     280     786 
   Size:        280     506
 
BsiHKAI G_wGCw'C

Cuts at:      0     566     786 
   Size:        566     220
 
Bsp1286I G_dGCh'C

Cuts at:      0     566     786 
   Size:        566     220
 
BsrGI T'GTAC_A

Cuts at:      0     460     786 
   Size:        460     326
 
BstYI r'GATC_y

Cuts at:      0     340     404     786 
   Size:        340      64     382

  Fragments arranged by size: 

                 382     340      64
 
BtsI GCAGTG_nn'

Cuts at:      0     767     786 
   Size:        767      19
 
DraI TTT'AAA

Cuts at:      0     389     786 
   Size:        389     397
 
EaeI y'GGCC_r

Cuts at:      0     117     443     587     722     786 
   Size:        117     326     144     135      64

  Fragments arranged by size: 

                 326     144     135     117      64
 
EarI CTCTTCn'nnn_

Cuts at:      0     251     786 
   Size:        251     535
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     145     786 
   Size:        145     641
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     676     786 
   Size:        112     564     110

  Fragments arranged by size: 

                 564     112     110
 
EcoO109I rG'GnC_Cy

Cuts at:      0     329     786 
   Size:        329     457
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     276     308     661     693     786 
   Size:        276      32     353      32      93

  Fragments arranged by size: 

                 353     276      93      32      32
 
HindIII A'AGCT_T

Cuts at:      0     379     786 
   Size:        379     407
 
KpnI G_GTAC'C

Cuts at:      0     541     786 
   Size:        541     245
 
MscI TGG'CCA

Cuts at:      0     119     445     589     786 
   Size:        119     326     144     197

  Fragments arranged by size: 

                 326     197     144     119
 
MspA1I CmG'CkG

Cuts at:      0     145     426     466     786 
   Size:        145     281      40     320

  Fragments arranged by size: 

                 320     281     145      40
 
PpuMI rG'GwC_Cy

Cuts at:      0     329     786 
   Size:        329     457
 
PstI C_TGCA'G

Cuts at:      0     471     786 
   Size:        471     315
 
PvuII CAG'CTG

Cuts at:      0     145     426     466     786 
   Size:        145     281      40     320

  Fragments arranged by size: 

                 320     281     145      40
 
SfcI C'TryA_G

Cuts at:      0     467     786 
   Size:        467     319
 
SmlI C'TyrA_G

Cuts at:      0     295     786 
   Size:        295     491
 
SspI AAT'ATT

Cuts at:      0     219     786 
   Size:        219     567
 
StyI C'CwwG_G

Cuts at:      0       5     230     665     780     786 
   Size:          5     225     435     115       6

  Fragments arranged by size: 

                 435     225     115       6       5
 
TatI w'GTAC_w

Cuts at:      0     460     786 
   Size:        460     326
 

 Enzymes that do cut:

   Acc65I     AcuI     AseI    BamHI     BanI     BbsI    BciVI    BglII
    BpuEI  BsiHKAI Bsp1286I    BsrGI    BstYI     BtsI     DraI     EaeI
     EarI     EciI  Eco57MI EcoO109I    Hin4I  HindIII     KpnI     MscI
   MspA1I    PpuMI     PstI    PvuII     SfcI     SmlI     SspI     StyI
     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI    AsiSI     AvaI    AvrII     BaeI    BanII     BbeI    BbvCI
     BcgI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI   DraIII     DrdI     EagI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
     HpaI     KasI     MfeI     MluI     MmeI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI