################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BclI BcnI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BssHII BstAPI BstBI BstEII BstPI BstUI BstYI Bsu36I CelII CfoI ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI Ksp632I KspI MaeI MfeI MflI MluI MroI MseI MspA1I MunI MvnI NarI NciI NdeI NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspI StuI SwaI Tru9I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccI 556 GTATAC GT^[AC][GT]AC AflIII 552 ACACGT A^C[GA][CT]GT AlwI 396 GATCC GGATC(4/5) AlwNI 511 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG AspI 217 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC BglI 130 GCCACTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 44 CTGGAG CTGGAG(16/14) BsaAI 553 CACGTA [CT]AC^GT[GA] BsaBI 382 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmAI 47 GAGAC GTCTC(1/5) Bsp1407I 444 TGTACA T^GTACA BsrFI 66 GCCGGC [GA]^CCGG[CT] BsrGI 444 TGTACA T^GTACA BsrI 633 CCAGT ACTGG(1/-1) BssSI 99 CACGAG CACGAG(-5/-1) Bst1107I 556 GTATAC GTA^TAC BstF5I 381 GGATG GGATG(2/0) BstZ17I 556 GTATAC GTA^TAC Cfr10I 66 GCCGGC [GA]^CCGG[CT] DdeI 246 CTCAG C^T[ACGT]AG DraII 512 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO109I 512 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoRV 374 GATATC GAT^ATC EcoT14I 363 CCATGG C^C[AT][AT]GG EcoT22I 58 ATGCAT ATGCA^T FauI 70 GCGGG CCCGC(4/6) FokI 381 GGATG GGATG(9/13) HapII 67 CCGG C^CGG HincII 598 GTCAAC GT[CT]^[GA]AC HindII 598 GTCAAC GT[CT]^[GA]AC HpaII 67 CCGG C^CGG MaeIII 520 GTGAC ^GT[ACGT]AC MamI 382 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspI 67 CCGG C^CGG MwoI 130 GCCACTCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NaeI 66 GCCGGC GCC^GGC NcoI 363 CCATGG C^CATGG NgoMIV 66 GCCGGC G^CCGGC NsiI 58 ATGCAT ATGCA^T NspI 60 GCATGT [GA]CATG^[CT] PflFI 217 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC SbfI 591 CCTGCAGG CCTGCA^GG SfaNI 318 GCATC GCATC(5/9) SmlI 661 CTTGAG C^T[CT][GA]AG Sse8387I 591 CCTGCAGG CCTGCA^GG SspBI 444 TGTACA T^GTACA StyI 363 CCATGG C^C[AT][AT]GG TaqI 467 TCGA T^CGA Tsp45I 520 GTGAC ^GT[GC]AC TspRI 524 CAGTG CA[GC]TG[ACGT][ACGT]^ Tth111I 217 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 467 TCGA T^CGA XcmI 125 CCACAGCCACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AciI 105 CCGC CCGC(-3/-1) 70 GCGG AluI 109 AGCT AG^CT 482 AGCT AvaII 3 GGACC G^G[AT]CC 95 GGACC BalI 80 TGGCCA TGG^CCA 161 TGGCCA BanII 72 GGGCTC G[GA]GC[CT]^C 275 GGGCTC BbsI 250 GAAGAC GAAGAC(2/6) 151 GTCTTC BciVI 624 GGATAC GTATCC(6/5) 263 GTATCC BmyI 72 GGGCTC G[AGT]GC[ACT]^C 275 GGGCTC BpuAI 250 GAAGAC GAAGAC(2/6) 151 GTCTTC BsmFI 94 GGGAC GGGAC(10/14) 223 GTCCC BsmI 539 GAATGC GAATGC(1/-1) 356 GCATTC Bsp1286I 72 GGGCTC G[AGT]GC[ACT]^C 275 GGGCTC BspMI 590 ACCTGC ACCTGC(4/8) 594 GCAGGT DpnII 396 GATC ^GATC 469 GATC DsaI 77 CCGTGG C^C[GA][CT]GG 363 CCATGG EaeI 80 TGGCCA [CT]^GGCC[GA] 161 TGGCCA MaeII 221 ACGT A^CGT 554 ACGT MboI 396 GATC ^GATC 469 GATC MluNI 80 TGGCCA TGG^CCA 161 TGGCCA MscI 80 TGGCCA TGG^CCA 161 TGGCCA NdeII 396 GATC ^GATC 469 GATC Sau3AI 396 GATC ^GATC 469 GATC TfiI 387 GAATC G^A[AT]TC 564 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AfaI BbvI BsaJI BsiYI BslI BssKI BstNI BstXI Cac8I Cfr13I EcoRII Fnu4HI HaeIII HinfI HphI ItaI MboII MnlI MslI MvaI NlaIII NlaIV PleI PstI RsaI Sau96I ScrFI SfcI TseI Tsp509I