################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII CfoI ClaI CpoI DraI DraIII EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MaeIII MamI MboII MfeI MflI MluI MluNI MroI MscI MseI MslI MspA1I MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI SwaI Tru9I Tsp45I Tth111I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AfaI 673 GTAC GT^AC AlwNI 643 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 101 GGGCCC GGGCC^C BsgI 324 CTGCAC GTGCAG(16/14) BsmAI 387 GAGAC GTCTC(1/5) BspMI 333 GCAGGT ACCTGC(4/8) BsrFI 8 ACCGGC [GA]^CCGG[CT] Cfr10I 8 ACCGGC [GA]^CCGG[CT] DpnII 399 GATC ^GATC DrdI 652 GACCCCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC HinfI 667 GATTC G^A[ACGT]TC MaeII 31 ACGT A^CGT MboI 399 GATC ^GATC NcoI 105 CCATGG C^CATGG NdeI 541 CATATG CA^TATG NdeII 399 GATC ^GATC NspI 614 GCATGC [GA]CATG^[CT] PflMI 105 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsaI 673 GTAC GT^AC Sau3AI 399 GATC ^GATC SmaI 98 CCCGGG CCC^GGG SphI 614 GCATGC GCATG^C TaqI 461 TCGA T^CGA TfiI 667 GATTC G^A[AT]TC Tsp509I 577 AATT ^AATT TthHB8I 461 TCGA T^CGA Van91I 105 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 98 CCCGGG C^CCGGG XmaI 98 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AocI 71 CCTTAGG CC^T[ACGT]AGG 360 CCTGAGG AvaI 98 CCCGGG C^[CT]CG[GA]G 602 CTCGGG BbvCI 125 CCTCAGC CCTCAGC(-5/-2) 349 GCTGAGG BcnI 98 CCCGG CC^[GC]GG 99 CCGGG BfaI 421 CTAG C^TAG 730 CTAG BglI 88 GCCACCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 728 GCCTAGAGGGC BpmI 743 CTCCAG CTGGAG(16/14) 112 CTGGAG BsoBI 98 CCCGGG C^[CT]CG[GA]G 602 CTCGGG Bsu36I 71 CCTTAGG CC^T[ACGT]AGG 360 CCTGAGG DraII 437 GGGGCCT [GA]G^G[ACGT]CC[CT] 597 GGGGCCT Eco81I 71 CCTTAGG CC^T[ACGT]AGG 360 CCTGAGG EcoO109I 437 GGGGCCT [GA]G^G[ACGT]CC[CT] 597 GGGGCCT MaeI 421 CTAG C^TAG 730 CTAG NciI 98 CCCGG CC^[GC]GG 99 CCGGG PstI 13 CTGCAG CTGCA^G 238 CTGCAG SfaNI 268 GATGC GCATC(5/9) 428 GCATC StuI 80 AGGCCT AGG^CCT 524 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI AluI AvaII BanII BbvI BmyI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DsaI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HpaII ItaI MnlI MspI MvaI MwoI NlaIII NlaIV Sau96I ScrFI SfcI StyI TseI TspRI