(Circular) (Six-base) MAPSORT of: pFN21AE5281.seq Check: 9313 from: 1 to: 5458 pFN21AE5281 5458 bp With 182 enzymes: SgfI * January 30, 2014 17:16 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2329 2329 Size: 5458 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3268 278 Size: 53 83 186 936 1732 2468 Fragments arranged by size: 2468 1732 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2801 1937 Size: 864 4594 Acc65I G'GTAC_C Cuts at: 2779 2779 Size: 5458 AclI AA'CG_TT Cuts at: 1563 3585 3958 1563 Size: 2022 373 3063 Fragments arranged by size: 3063 2022 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2134 2302 3521 4905 5173 1632 Size: 502 168 1219 1384 268 1917 Fragments arranged by size: 1917 1384 1219 502 268 168 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5261 AflIII A'CryG_T Cuts at: 1203 4357 5334 1203 Size: 3154 977 1327 Fragments arranged by size: 3154 1327 977 AgeI A'CCGG_T Cuts at: 4289 4289 Size: 5458 AhdI GACnn_n'nnGTC Cuts at: 4187 4187 Size: 5458 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5458 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5458 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2679 2711 2679 Size: 32 5426 AlwNI CAG_nnn'CTG Cuts at: 2653 3201 4773 2653 Size: 548 1572 3338 Fragments arranged by size: 3338 1572 548 ApaI G_GGCC'C Cuts at: 2110 2110 Size: 5458 ApaLI G'TGCA_C Cuts at: 3515 4671 3515 Size: 1156 4302 ApoI r'AATT_y Cuts at: 1493 2767 2993 3145 5254 1493 Size: 1274 226 152 2109 1697 Fragments arranged by size: 2109 1697 1274 226 152 AseI AT'TA_AT Cuts at: 160 1032 4012 160 Size: 872 2980 1606 Fragments arranged by size: 2980 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5458 AvaI C'yCGr_G Cuts at: 1870 1943 2103 2607 2783 1870 Size: 73 160 504 176 4545 Fragments arranged by size: 4545 504 176 160 73 BamHI G'GATC_C Cuts at: 2788 3155 2788 Size: 367 5091 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2779 4234 618 Size: 325 217 627 992 1455 1842 Fragments arranged by size: 1842 1455 992 627 325 217 BanII G_rGCy'C Cuts at: 1391 2110 2130 2170 2744 2777 1391 Size: 719 20 40 574 33 4072 Fragments arranged by size: 4072 719 574 40 33 20 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4487 BbvCI CC'TCA_GC Cuts at: 2131 2355 2131 Size: 224 5234 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3649 3683 1903 Size: 34 1712 34 3678 Fragments arranged by size: 3678 1712 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3350 4560 3350 Size: 1210 4248 BclI T'GATC_A Cuts at: 1548 1548 Size: 5458 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2099 2739 4069 Size: 107 122 71 631 605 427 640 1330 Cuts at: 4069 136 Size: 1525 Fragments arranged by size: 1525 1330 640 631 605 427 122 107 71 BglII A'GATC_T Cuts at: 5453 5453 Size: 5458 BlpI GC'TnA_GC Cuts at: 2875 2875 Size: 5458 Bme1580I G_kGCm'C Cuts at: 1790 2110 3519 4675 1790 Size: 320 1409 1156 2573 Fragments arranged by size: 2573 1409 1156 320 BmgBI CAC'GTC Cuts at: 1318 2037 1318 Size: 719 4739 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3189 4147 442 Size: 416 53 83 399 24 1772 958 1753 Fragments arranged by size: 1772 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5458 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2138 2733 4118 1239 Size: 899 595 1385 2579 Fragments arranged by size: 2579 1385 899 595 Bpu10I CC'TnA_GC Cuts at: 2060 2131 2198 2277 2355 2060 Size: 71 67 79 78 5163 Fragments arranged by size: 5163 79 78 71 67 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3583 4448 4746 3583 Size: 865 298 4295 Fragments arranged by size: 4295 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4121 882 Size: 617 2622 2219 Fragments arranged by size: 2622 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3249 493 Size: 711 2045 2702 Fragments arranged by size: 2702 2045 711 BsaBI GATnn'nnATC Cuts at: 5452 5452 Size: 5458 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3265 3647 4282 275 Size: 53 83 186 936 1732 382 635 1451 Fragments arranged by size: 1732 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3891 4289 4563 4710 5121 3891 Size: 398 274 147 411 4228 Fragments arranged by size: 4228 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2679 2709 1178 Size: 30 165 30 1276 30 3927 Fragments arranged by size: 3927 1276 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5458 BseYI C'CCAG_C Cuts at: 4661 5157 4661 Size: 496 4962 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2314 1163 Size: 573 578 4307 Fragments arranged by size: 4307 578 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2908 3669 4697 1540 Size: 275 186 907 761 1028 2301 Fragments arranged by size: 2301 1028 907 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3519 3604 4675 3519 Size: 85 1071 4302 Fragments arranged by size: 4302 1071 85 BsmI GAATG_Cn' Cuts at: 2969 3062 2969 Size: 93 5365 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2110 2130 2170 2744 2777 3519 3604 Size: 399 320 20 40 574 33 742 85 Cuts at: 3604 4675 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 574 399 320 85 40 33 20 BspEI T'CCGG_A Cuts at: 5121 5121 Size: 5458 BspHI T'CATG_A Cuts at: 3347 5077 3347 Size: 1730 3728 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2329 2813 844 Size: 1485 484 3489 Fragments arranged by size: 3489 1485 484 BsrBI CCG'CTC Cuts at: 1394 1823 3345 1394 Size: 429 1522 3507 Fragments arranged by size: 3507 1522 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3953 4127 65 Size: 1183 2705 174 1396 Fragments arranged by size: 2705 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2013 4102 4289 1700 Size: 110 203 2089 187 2869 Fragments arranged by size: 2869 2089 203 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5458 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5458 BssSI C'ACGA_G Cuts at: 3518 4530 3518 Size: 1012 4446 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5458 BstBI TT'CG_AA Cuts at: 4262 4262 Size: 5458 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5458 BstYI r'GATC_y Cuts at: 1972 2788 3155 3540 3557 5009 5095 5107 5453 Size: 816 367 385 17 1452 86 12 346 Cuts at: 5453 1972 Size: 1977 Fragments arranged by size: 1977 1452 816 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2077 2366 2077 Size: 289 5169 BtgI C'CryG_G Cuts at: 513 1065 2004 2110 2203 2661 513 Size: 552 939 106 93 458 3310 Fragments arranged by size: 3310 939 552 458 106 93 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2278 487 Size: 683 56 1052 3667 Fragments arranged by size: 3667 1052 683 56 BtsI GCAGTG_nn' Cuts at: 2406 2974 3768 3788 2406 Size: 568 794 20 4076 Fragments arranged by size: 4076 794 568 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5458 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5423 DraI TTT'AAA Cuts at: 2762 2762 Size: 5458 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2663 4465 809 Size: 507 1347 1802 1802 Fragments arranged by size: 1802 1802 1347 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2905 3794 5423 Size: 54 1425 50 275 18 1075 889 1629 Cuts at: 5423 8 Size: 43 Fragments arranged by size: 1629 1425 1075 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2905 1537 Size: 275 1093 4090 Fragments arranged by size: 4090 1093 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3388 1353 Size: 2035 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2057 4045 4419 4565 387 Size: 1064 138 468 1988 374 146 1280 Fragments arranged by size: 1988 1280 1064 468 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2134 2138 2302 2733 3521 4118 4905 Size: 393 502 4 164 431 788 597 787 Cuts at: 4905 5173 1239 Size: 268 1524 Fragments arranged by size: 1524 788 787 597 502 431 393 268 164 4 EcoO109I rG'GnC_Cy Cuts at: 2443 2603 2443 Size: 160 5298 EcoRI G'AATT_C Cuts at: 2767 3145 2767 Size: 378 5080 FspI TGC'GCA Cuts at: 3964 3964 Size: 5458 HaeII r_GCGC'y Cuts at: 1678 4605 1678 Size: 2927 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4099 4131 4173 4205 1373 Size: 32 135 32 2527 32 42 32 2626 Fragments arranged by size: 2626 2527 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2802 3048 669 Size: 988 281 864 246 3079 Fragments arranged by size: 3079 988 864 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5458 HpaI GTT'AAC Cuts at: 3048 3048 Size: 5458 KpnI G_GTAC'C Cuts at: 2783 2783 Size: 5458 MfeI C'AATT_G Cuts at: 3057 3057 Size: 5458 MluI A'CGCG_T Cuts at: 5334 5334 Size: 5458 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2325 2433 4572 4756 1305 Size: 1020 108 2139 184 2007 Fragments arranged by size: 2139 2007 1020 184 108 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3636 Fragments arranged by size: 3636 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3416 3775 3934 518 Size: 648 2250 359 159 2042 Fragments arranged by size: 2250 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2874 3551 4699 4944 1606 Size: 1268 677 1148 245 2120 Fragments arranged by size: 2120 1268 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2110 513 Size: 552 939 106 3861 Fragments arranged by size: 3861 939 552 106 NdeI CA'TA_TG Cuts at: 387 2547 387 Size: 2160 3298 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5458 NotI GC'GGCC_GC Cuts at: 2905 2905 Size: 5458 NspI r_CATG'y Cuts at: 1132 1229 2623 2816 4361 1132 Size: 97 1394 193 1545 2229 Fragments arranged by size: 2229 1545 1394 193 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5458 PciI A'CATG_T Cuts at: 4357 4357 Size: 5458 PflMI CCAn_nnn'nTGG Cuts at: 1108 2116 1108 Size: 1008 4450 PfoI T'CCnGG_A Cuts at: 2268 2523 2268 Size: 255 5203 PmeI GTTT'AAAC Cuts at: 2762 2762 Size: 5458 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2679 2711 3527 3559 5040 5072 2679 Size: 32 816 32 1481 32 3065 Fragments arranged by size: 3065 1481 816 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5458 PsiI TTA'TAA Cuts at: 2310 3028 2310 Size: 718 4740 PspOMI G'GGCC_C Cuts at: 2106 2106 Size: 5458 PstI C_TGCA'G Cuts at: 830 2022 2247 2810 830 Size: 1192 225 563 3478 Fragments arranged by size: 3478 1192 563 225 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5458 SalI G'TCGA_C Cuts at: 1936 2800 1936 Size: 864 4594 SbfI CC_TGCA'GG Cuts at: 2810 2810 Size: 5458 ScaI AGT'ACT Cuts at: 3706 5418 3706 Size: 1712 3746 SfcI C'TryA_G Cuts at: 826 1045 2018 2243 2283 2806 3941 4622 4813 Size: 219 973 225 40 523 1135 681 191 Cuts at: 4813 826 Size: 1471 Fragments arranged by size: 1471 1135 973 681 523 225 219 191 40 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5458 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5458 SmaI CCC'GGG Cuts at: 1872 2105 2785 1872 Size: 233 680 4545 Fragments arranged by size: 4545 680 233 SmlI C'TyrA_G Cuts at: 820 1017 1943 3562 4463 4725 820 Size: 197 926 1619 901 262 1553 Fragments arranged by size: 1619 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3249 493 Size: 2756 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5458 SphI G_CATG'C Cuts at: 1132 2623 2816 1132 Size: 1491 193 3774 Fragments arranged by size: 3774 1491 193 SrfI GCCC'GGGC Cuts at: 2105 2105 Size: 5458 SspI AAT'ATT Cuts at: 5 52 3382 5 Size: 47 3330 2081 Fragments arranged by size: 3330 2081 47 StuI AGG'CCT Cuts at: 1517 2087 2531 1517 Size: 570 444 4444 Fragments arranged by size: 4444 570 444 StyI C'CwwG_G Cuts at: 513 1065 2004 2097 2110 2446 2897 513 Size: 552 939 93 13 336 451 3074 Fragments arranged by size: 3074 939 552 451 336 93 13 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2433 3485 3502 3655 3840 4285 325 Size: 1255 853 1052 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1052 853 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3704 5416 96 Size: 274 80 33 51 1443 1727 1712 138 Fragments arranged by size: 1727 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5418 5450 5418 Size: 32 5426 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5458 XbaI T'CTAG_A Cuts at: 2794 2794 Size: 5458 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5458 XmaI C'CCGG_G Cuts at: 1870 2103 2783 1870 Size: 233 680 4545 Fragments arranged by size: 4545 680 233 XmnI GAAnn'nnTTC Cuts at: 3587 3587 Size: 5458 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3266 276 Size: 53 83 186 936 1732 2468 Fragments arranged by size: 2468 1732 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SwaI XcmI