(Linear) (Six-base) MAPSORT of: KIEE5281.seq Check: 4513 from: 1 to: 753 KIEE5281.orf With 182 enzymes: SgfI * January 29, 2014 11:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 324 753 Size: 324 429 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 129 297 753 Size: 129 168 456 Fragments arranged by size: 456 168 129 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 674 706 753 Size: 674 32 47 Fragments arranged by size: 674 47 32 AlwNI CAG_nnn'CTG Cuts at: 0 648 753 Size: 648 105 ApaI G_GGCC'C Cuts at: 0 105 753 Size: 105 648 AvaI C'yCGr_G Cuts at: 0 98 602 753 Size: 98 504 151 Fragments arranged by size: 504 151 98 BanII G_rGCy'C Cuts at: 0 105 125 165 739 753 Size: 105 20 40 574 14 Fragments arranged by size: 574 105 40 20 14 BbvCI CC'TCA_GC Cuts at: 0 126 350 753 Size: 126 224 403 Fragments arranged by size: 403 224 126 BglI GCCn_nnn'nGGC Cuts at: 0 94 734 753 Size: 94 640 19 Fragments arranged by size: 640 94 19 Bme1580I G_kGCm'C Cuts at: 0 105 753 Size: 105 648 BmgBI CAC'GTC Cuts at: 0 32 753 Size: 32 721 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 133 728 753 Size: 133 595 25 Fragments arranged by size: 595 133 25 Bpu10I CC'TnA_GC Cuts at: 0 55 126 193 272 350 753 Size: 55 71 67 79 78 403 Fragments arranged by size: 403 79 78 71 67 55 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 674 704 753 Size: 674 30 49 Fragments arranged by size: 674 49 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 309 753 Size: 309 444 Bsp1286I G_dGCh'C Cuts at: 0 105 125 165 739 753 Size: 105 20 40 574 14 Fragments arranged by size: 574 105 40 20 14 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 324 753 Size: 324 429 BsrFI r'CCGG_y Cuts at: 0 8 753 Size: 8 745 Bsu36I CC'TnA_GG Cuts at: 0 72 361 753 Size: 72 289 392 Fragments arranged by size: 392 289 72 BtgI C'CryG_G Cuts at: 0 105 198 656 753 Size: 105 93 458 97 Fragments arranged by size: 458 105 97 93 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 273 753 Size: 273 480 BtsI GCAGTG_nn' Cuts at: 0 401 753 Size: 401 352 DrdI GACnn_nn'nnGTC Cuts at: 0 658 753 Size: 658 95 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 52 753 Size: 52 701 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 129 133 297 728 753 Size: 129 4 164 431 25 Fragments arranged by size: 431 164 129 25 4 EcoO109I rG'GnC_Cy Cuts at: 0 438 598 753 Size: 438 160 155 Fragments arranged by size: 438 160 155 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 320 428 753 Size: 320 108 325 Fragments arranged by size: 325 320 108 NcoI C'CATG_G Cuts at: 0 105 753 Size: 105 648 NdeI CA'TA_TG Cuts at: 0 542 753 Size: 542 211 NspI r_CATG'y Cuts at: 0 618 753 Size: 618 135 PflMI CCAn_nnn'nTGG Cuts at: 0 111 753 Size: 111 642 PfoI T'CCnGG_A Cuts at: 0 263 518 753 Size: 263 255 235 Fragments arranged by size: 263 255 235 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 674 706 753 Size: 674 32 47 Fragments arranged by size: 674 47 32 PsiI TTA'TAA Cuts at: 0 305 753 Size: 305 448 PspOMI G'GGCC_C Cuts at: 0 101 753 Size: 101 652 PstI C_TGCA'G Cuts at: 0 17 242 753 Size: 17 225 511 Fragments arranged by size: 511 225 17 SfcI C'TryA_G Cuts at: 0 13 238 278 753 Size: 13 225 40 475 Fragments arranged by size: 475 225 40 13 SmaI CCC'GGG Cuts at: 0 100 753 Size: 100 653 SphI G_CATG'C Cuts at: 0 618 753 Size: 618 135 SrfI GCCC'GGGC Cuts at: 0 100 753 Size: 100 653 StuI AGG'CCT Cuts at: 0 82 526 753 Size: 82 444 227 Fragments arranged by size: 444 227 82 StyI C'CwwG_G Cuts at: 0 92 105 441 753 Size: 92 13 336 312 Fragments arranged by size: 336 312 92 13 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 428 753 Size: 428 325 XmaI C'CCGG_G Cuts at: 0 98 753 Size: 98 655 Enzymes that do cut: AarI AcuI AloI AlwNI ApaI AvaI BanII BbvCI BglI Bme1580I BmgBI BpmI Bpu10I BsaXI BsgI Bsp1286I BspMI BsrFI Bsu36I BtgI BtgZI BtsI DrdI EciI Eco57MI EcoO109I MmeI NcoI NdeI NspI PflMI PfoI PpiI PsiI PspOMI PstI SfcI SmaI SphI SrfI StuI StyI TaqII XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I ClaI CspCI DraI DraIII EaeI EagI EarI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MscI MslI MspA1I NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpuMI PshAI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SspI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI