################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AviII AvrII BalI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BfaI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BspDI BspEI BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRII EcoRV FauI FseI FspI HgaI Hin1I HincII HindII HpaI KasI KpnI Ksp632I MaeI MaeII MamI MfeI MluI MluNI MroI MscI MunI MvaI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI TaqI TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccII 129 CGCG CG^CG AocI 405 CCTCAGG CC^T[ACGT]AGG AspI 290 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 115 CCCGAG C^[CT]CG[GA]G AvaII 48 GGACC G^G[AT]CC BamHI 147 GGATCC G^GATCC BanII 112 GAGCCC G[GA]GC[CT]^C BbvI 610 GCAGC GCAGC(8/12) BclI 573 TGATCA T^GATCA BcnI 83 CCGGG CC^[GC]GG BglII 1189 AGATCT A^GATCT BmyI 112 GAGCCC G[AGT]GC[ACT]^C BsmFI 1544 GTCCC GGGAC(10/14) BsmI 1300 GAATGC GAATGC(1/-1) BsoBI 115 CCCGAG C^[CT]CG[GA]G Bsp1286I 112 GAGCCC G[AGT]GC[ACT]^C BspHI 1884 TCATGA T^CATGA BsrDI 1304 GCAATG GCAATG(2/0) BssKI 83 CCGGG ^CC[ACGT]GG BstUI 129 CGCG CG^CG Bsu36I 405 CCTCAGG CC^T[ACGT]AGG Cac8I 121 GCCAGC GC[ACGT]^[ACGT]GC CfoI 125 GCGC GCG^C Cfr13I 48 GGACC G^G[ACGT]CC DraI 1576 TTTAAA TTT^AAA Eco81I 405 CCTCAGG CC^T[ACGT]AGG EcoT22I 1838 ATGCAT ATGCA^T FbaI 573 TGATCA T^GATCA HaeII 124 AGCGCC [GA]GCGC^[CT] HhaI 125 GCGC GCG^C HinP1I 125 GCGC G^CGC KspI 128 CCGCGG CCGC^GG MvnI 129 CGCG CG^CG NciI 83 CCGGG CC^[GC]GG NlaIV 147 GGATCC GG[ACGT]^[ACGT]CC NsiI 1838 ATGCAT ATGCA^T PflFI 290 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC RcaI 1884 TCATGA T^CATGA SacII 128 CCGCGG CCGC^GG Sau96I 48 GGACC G^G[ACGT]CC ScrFI 83 CCGGG CC^[ACGT]GG StuI 1590 AGGCCT AGG^CCT TfiI 24 GATTC G^A[AT]TC TseI 610 GCAGC G^C[AT]GC Tth111I 290 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AfaI 1012 GTAC GT^AC 1684 GTAC AlwNI 22 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG 453 CAGTGACTG BsaI 40 GAGACC GGTCTC(1/5) 6 GGTCTC BsaWI 50 ACCGGA [AT]^CCGG[AT] 1030 ACCGGA BseRI 403 CTCCTC GAGGAG(10/8) 54 GAGGAG Bsp1407I 1011 TGTACA T^GTACA 1683 TGTACA BsrGI 1011 TGTACA T^GTACA 1683 TGTACA BstYI 147 GGATCC [GA]^GATC[CT] 1189 AGATCT EcoRI 1106 GAATTC G^AATTC 1358 GAATTC EcoT14I 268 CCATGG C^C[AT][AT]GG 1071 CCATGG HindIII 860 AAGCTT A^AGCTT 1403 AAGCTT MflI 147 GGATCC [GA]^GATC[CT] 1189 AGATCT MspA1I 12 CAGCGG C[AC]G^C[GT]G 128 CCGCGG MwoI 121 GCCAGCGCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1741 GCCTTTAATGC NcoI 268 CCATGG C^CATGG 1071 CCATGG PleI 920 GACTC GAGTC(4/5) 532 GAGTC RsaI 1012 GTAC GT^AC 1684 GTAC SspBI 1011 TGTACA T^GTACA 1683 TGTACA StyI 268 CCATGG C^C[AT][AT]GG 1071 CCATGG XhoII 147 GGATCC [GA]^GATC[CT] 1189 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AciI AcsI AflIII AluI AlwI ApoI Asp700I BpmI BsaJI BsiYI BslI BsmAI BspLU11I BsrI BstF5I DdeI DpnII DsaI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MseI MslI MspI NdeI NdeII NlaIII NspI Sau3AI SfaNI SmlI Tru9I Tsp45I Tsp509I TspRI XmnI