(Circular) (Six-base) MAPSORT of: pFN21AB7862.seq Check: 6985 from: 1 to: 6595 pFN21AB7862 6595 bp With 182 enzymes: SgfI * July 6, 2012 17:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4405 278 Size: 53 83 186 936 2869 2468 Fragments arranged by size: 2869 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3938 1937 Size: 2001 4594 Acc65I G'GTAC_C Cuts at: 3916 3916 Size: 6595 AclI AA'CG_TT Cuts at: 1563 4722 5095 1563 Size: 3159 373 3063 Fragments arranged by size: 3159 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3145 4658 6042 6310 1632 Size: 1513 1513 1384 268 1917 Fragments arranged by size: 1917 1513 1513 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6398 AflIII A'CryG_T Cuts at: 1203 2503 3187 3271 3859 5494 6471 1203 Size: 1300 684 84 588 1635 977 1327 Fragments arranged by size: 1635 1327 1300 977 684 588 84 AgeI A'CCGG_T Cuts at: 5426 5426 Size: 6595 AhdI GACnn_n'nnGTC Cuts at: 5324 5324 Size: 6595 AleI CACnn'nnGTG Cuts at: 1166 3204 1166 Size: 2038 4557 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6595 AlwNI CAG_nnn'CTG Cuts at: 2032 2463 4338 5910 2032 Size: 431 1875 1572 2717 Fragments arranged by size: 2717 1875 1572 431 ApaLI G'TGCA_C Cuts at: 4652 5808 4652 Size: 1156 5439 ApoI r'AATT_y Cuts at: 1493 2531 2550 2563 2796 3111 3363 3904 4130 Size: 1038 19 13 233 315 252 541 226 Cuts at: 4130 4282 6391 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1038 541 315 252 233 226 152 19 13 AseI AT'TA_AT Cuts at: 160 1032 5149 160 Size: 872 4117 1606 Fragments arranged by size: 4117 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6595 AvaI C'yCGr_G Cuts at: 1870 1943 2120 3920 1870 Size: 73 177 1800 4545 Fragments arranged by size: 4545 1800 177 73 BamHI G'GATC_C Cuts at: 2152 3925 4292 2152 Size: 1773 367 4455 Fragments arranged by size: 4455 1773 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3916 5371 618 Size: 325 217 627 2129 1455 1842 Fragments arranged by size: 2129 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2121 3914 1391 Size: 730 1793 4072 Fragments arranged by size: 4072 1793 730 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5624 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4786 4820 1903 Size: 34 2849 34 3678 Fragments arranged by size: 3678 2849 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4487 5697 4487 Size: 1210 5385 BclI T'GATC_A Cuts at: 1548 2578 1548 Size: 1030 5565 BfrBI ATG'CAT Cuts at: 3845 3845 Size: 6595 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5206 136 Size: 107 122 71 631 605 3534 1525 Fragments arranged by size: 3534 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3194 6590 3194 Size: 3396 3199 BlpI GC'TnA_GC Cuts at: 4012 4012 Size: 6595 Bme1580I G_kGCm'C Cuts at: 1790 4656 5812 1790 Size: 2866 1156 2573 Fragments arranged by size: 2866 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6595 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2292 2483 2961 Size: 416 53 83 399 24 875 191 478 Cuts at: 2961 3717 4326 5284 442 Size: 756 609 958 1753 Fragments arranged by size: 1753 958 875 756 609 478 416 399 191 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6595 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1999 2120 2210 2826 3141 3645 3813 5255 Size: 760 121 90 616 315 504 168 1442 Cuts at: 5255 1239 Size: 2579 Fragments arranged by size: 2579 1442 760 616 504 315 168 121 90 Bpu10I CC'TnA_GC Cuts at: 2834 2834 Size: 6595 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2424 2569 2847 4720 5585 5883 2424 Size: 145 278 1873 865 298 3136 Fragments arranged by size: 3136 1873 865 298 278 145 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2017 2039 5258 882 Size: 617 518 22 3219 2219 Fragments arranged by size: 3219 2219 617 518 22 BsaAI yAC'GTr Cuts at: 493 1204 4386 493 Size: 711 3182 2702 Fragments arranged by size: 3182 2702 711 BsaBI GATnn'nnATC Cuts at: 6589 6589 Size: 6595 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4402 4784 5419 275 Size: 53 83 186 936 2869 382 635 1451 Fragments arranged by size: 2869 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2055 3035 5028 5426 5700 5847 6258 2055 Size: 980 1993 398 274 147 411 2392 Fragments arranged by size: 2392 1993 980 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2050 2080 2231 2261 1178 Size: 30 165 30 647 30 151 30 5512 Fragments arranged by size: 5512 647 165 151 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2074 2399 1186 Size: 888 325 5382 Fragments arranged by size: 5382 888 325 BseYI C'CCAG_C Cuts at: 2828 5798 6294 2828 Size: 2970 496 3129 Fragments arranged by size: 3129 2970 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6022 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4045 4806 5834 1540 Size: 275 186 2044 761 1028 2301 Fragments arranged by size: 2301 2044 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4656 4741 5812 4656 Size: 85 1071 5439 Fragments arranged by size: 5439 1071 85 BsmI GAATG_Cn' Cuts at: 3311 4106 4199 3311 Size: 795 93 5707 Fragments arranged by size: 5707 795 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2121 3914 4656 4741 5812 1391 Size: 399 331 1793 742 85 1071 2174 Fragments arranged by size: 2174 1793 1071 742 399 331 85 BspEI T'CCGG_A Cuts at: 6258 6258 Size: 6595 BspHI T'CATG_A Cuts at: 3889 4484 6214 3889 Size: 595 1730 4270 Fragments arranged by size: 4270 1730 595 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3950 844 Size: 3106 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4482 1394 Size: 429 2659 3507 Fragments arranged by size: 3507 2659 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3316 5090 5264 65 Size: 1183 2068 1774 174 1396 Fragments arranged by size: 2068 1774 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5239 5426 1700 Size: 110 3429 187 2869 Fragments arranged by size: 3429 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3016 3688 96 Size: 2920 672 3003 Fragments arranged by size: 3003 2920 672 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6595 BssSI C'ACGA_G Cuts at: 4655 5667 4655 Size: 1012 5583 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6595 BstBI TT'CG_AA Cuts at: 5399 5399 Size: 6595 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6595 BstYI r'GATC_y Cuts at: 1972 2152 3194 3925 4292 4677 4694 6146 6232 Size: 180 1042 731 367 385 17 1452 86 Cuts at: 6232 6244 6590 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1042 731 385 367 346 180 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2411 2411 Size: 6595 BtgI C'CryG_G Cuts at: 513 1065 2049 2133 2273 3076 513 Size: 552 984 84 140 803 4032 Fragments arranged by size: 4032 984 803 552 140 84 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5856 Fragments arranged by size: 5856 683 56 BtsI GCAGTG_nn' Cuts at: 2464 4111 4905 4925 2464 Size: 1647 794 20 4134 Fragments arranged by size: 4134 1647 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6595 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3298 3333 529 Size: 35 2734 35 3791 Fragments arranged by size: 3791 2734 35 35 DraI TTT'AAA Cuts at: 3583 3899 3583 Size: 316 6279 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5602 809 Size: 507 4286 1802 Fragments arranged by size: 4286 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4042 4931 6560 Size: 54 1425 50 275 18 2212 889 1629 Cuts at: 6560 8 Size: 43 Fragments arranged by size: 2212 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4042 1537 Size: 275 2230 4090 Fragments arranged by size: 4090 2230 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4525 1353 Size: 3172 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5182 5556 5702 387 Size: 1064 138 3593 374 146 1280 Fragments arranged by size: 3593 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1999 2120 2210 2826 3141 3145 3645 Size: 393 367 121 90 616 315 4 500 Cuts at: 3645 3813 4658 5255 6042 6310 1239 Size: 168 845 597 787 268 1524 Fragments arranged by size: 1524 845 787 616 597 500 393 367 315 268 168 121 90 4 EcoRI G'AATT_C Cuts at: 3111 3363 3904 4282 3111 Size: 252 541 378 5424 Fragments arranged by size: 5424 541 378 252 FspI TGC'GCA Cuts at: 5101 5101 Size: 6595 HaeII r_GCGC'y Cuts at: 1678 2133 5742 1678 Size: 455 3609 2531 Fragments arranged by size: 3609 2531 455 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2231 2263 2687 2719 5236 Size: 32 135 32 659 32 424 32 2517 Cuts at: 5236 5268 5310 5342 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2517 659 424 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3939 4185 669 Size: 988 281 2001 246 3079 Fragments arranged by size: 3079 2001 988 281 246 HindIII A'AGCT_T Cuts at: 748 2865 3408 748 Size: 2117 543 3935 Fragments arranged by size: 3935 2117 543 HpaI GTT'AAC Cuts at: 4185 4185 Size: 6595 KpnI G_GTAC'C Cuts at: 3920 3920 Size: 6595 MfeI C'AATT_G Cuts at: 4194 4194 Size: 6595 MluI A'CGCG_T Cuts at: 6471 6471 Size: 6595 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2235 5709 5893 1305 Size: 930 3474 184 2007 Fragments arranged by size: 3474 2007 930 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4773 Fragments arranged by size: 4773 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2968 3052 3087 3204 3220 3288 3304 Size: 648 1802 84 35 117 16 68 16 Cuts at: 3304 3372 3540 4553 4912 5071 518 Size: 68 168 1013 359 159 2042 Fragments arranged by size: 2042 1802 1013 648 359 168 159 117 84 68 68 35 16 16 MspA1I CmG'CkG Cuts at: 1606 2019 2135 4011 4688 5836 6081 1606 Size: 413 116 1876 677 1148 245 2120 Fragments arranged by size: 2120 1876 1148 677 413 245 116 NcoI C'CATG_G Cuts at: 513 1065 2273 3076 513 Size: 552 1208 803 4032 Fragments arranged by size: 4032 1208 803 552 NdeI CA'TA_TG Cuts at: 387 2965 3049 3217 3301 3525 387 Size: 2578 84 168 84 224 3457 Fragments arranged by size: 3457 2578 224 168 84 84 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6595 NotI GC'GGCC_GC Cuts at: 4042 4042 Size: 6595 NsiI A_TGCA'T Cuts at: 3847 3847 Size: 6595 NspI r_CATG'y Cuts at: 1132 1229 2507 3092 3191 3275 3863 3953 5498 Size: 97 1278 585 99 84 588 90 1545 Cuts at: 5498 1132 Size: 2229 Fragments arranged by size: 2229 1545 1278 588 585 99 97 90 84 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6595 PciI A'CATG_T Cuts at: 2503 3187 3271 3859 5494 2503 Size: 684 84 588 1635 3604 Fragments arranged by size: 3604 1635 684 588 84 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6595 PmeI GTTT'AAAC Cuts at: 3899 3899 Size: 6595 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4664 4696 6177 6209 4664 Size: 32 1481 32 5050 Fragments arranged by size: 5050 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6595 PsiI TTA'TAA Cuts at: 3471 4165 3471 Size: 694 5901 PstI C_TGCA'G Cuts at: 830 3947 830 Size: 3117 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6595 SacII CC_GC'GG Cuts at: 2136 2136 Size: 6595 SalI G'TCGA_C Cuts at: 1936 3937 1936 Size: 2001 4594 SbfI CC_TGCA'GG Cuts at: 3947 3947 Size: 6595 ScaI AGT'ACT Cuts at: 4843 6555 4843 Size: 1712 4883 SfcI C'TryA_G Cuts at: 826 1045 3943 5078 5759 5950 826 Size: 219 2898 1135 681 191 1471 Fragments arranged by size: 2898 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6595 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6595 SmaI CCC'GGG Cuts at: 1872 3922 1872 Size: 2050 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2439 2584 2862 4699 5600 5862 Size: 197 926 496 145 278 1837 901 262 Cuts at: 5862 820 Size: 1553 Fragments arranged by size: 1837 1553 926 901 496 278 262 197 145 SnaBI TAC'GTA Cuts at: 493 4386 493 Size: 3893 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6595 SphI G_CATG'C Cuts at: 1132 3953 1132 Size: 2821 3774 SspI AAT'ATT Cuts at: 5 52 4519 5 Size: 47 4467 2081 Fragments arranged by size: 4467 2081 47 StuI AGG'CCT Cuts at: 1517 3597 1517 Size: 2080 4515 StyI C'CwwG_G Cuts at: 513 1065 2273 3076 4034 513 Size: 552 1208 803 958 3074 Fragments arranged by size: 3074 1208 958 803 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 1999 2842 4622 4639 4792 4977 5422 Size: 1255 419 843 1780 17 153 185 445 Cuts at: 5422 325 Size: 1498 Fragments arranged by size: 1780 1498 1255 843 445 419 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3016 3688 4841 Size: 274 80 33 51 1443 1039 672 1153 Cuts at: 4841 6553 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1153 1039 672 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2050 2082 6555 6587 2050 Size: 32 4473 32 2058 Fragments arranged by size: 4473 2058 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2298 1316 Size: 982 5613 XbaI T'CTAG_A Cuts at: 3931 3931 Size: 6595 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6595 XmaI C'CCGG_G Cuts at: 1870 3920 1870 Size: 2050 4545 XmnI GAAnn'nnTTC Cuts at: 2636 3057 3158 3410 3830 4724 2636 Size: 421 101 252 420 894 4507 Fragments arranged by size: 4507 894 421 420 252 101 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4403 276 Size: 53 83 186 936 2869 2468 Fragments arranged by size: 2869 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI