(Linear) (Six-base) MAPSORT of: KIBB7862.seq Check: 9637 from: 1 to: 1890 KIBB7862.orf With 182 enzymes: SgfI * July 3, 2012 16:30 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1140 1890 Size: 1140 750 AflIII A'CryG_T Cuts at: 0 498 1182 1266 1854 1890 Size: 498 684 84 588 36 Fragments arranged by size: 684 588 498 84 36 AleI CACnn'nnGTG Cuts at: 0 1199 1890 Size: 1199 691 AlwNI CAG_nnn'CTG Cuts at: 0 27 458 1890 Size: 27 431 1432 Fragments arranged by size: 1432 431 27 ApoI r'AATT_y Cuts at: 0 526 545 558 791 1106 1358 1890 Size: 526 19 13 233 315 252 532 Fragments arranged by size: 532 526 315 252 233 19 13 AvaI C'yCGr_G Cuts at: 0 115 1890 Size: 115 1775 BamHI G'GATC_C Cuts at: 0 147 1890 Size: 147 1743 BanII G_rGCy'C Cuts at: 0 116 1890 Size: 116 1774 BclI T'GATC_A Cuts at: 0 573 1890 Size: 573 1317 BfrBI ATG'CAT Cuts at: 0 1840 1890 Size: 1840 50 BglII A'GATC_T Cuts at: 0 1189 1890 Size: 1189 701 BmrI ACTGGGnnnn_n' Cuts at: 0 287 478 956 1712 1890 Size: 287 191 478 756 178 Fragments arranged by size: 756 478 287 191 178 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 115 205 821 1136 1640 1808 1890 Size: 115 90 616 315 504 168 82 Fragments arranged by size: 616 504 315 168 115 90 82 Bpu10I CC'TnA_GC Cuts at: 0 829 1890 Size: 829 1061 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 419 564 842 1890 Size: 419 145 278 1048 Fragments arranged by size: 1048 419 278 145 BsaI GGTCTCn'nnnn_ Cuts at: 0 12 34 1890 Size: 12 22 1856 Fragments arranged by size: 1856 22 12 BsaWI w'CCGG_w Cuts at: 0 50 1030 1890 Size: 50 980 860 Fragments arranged by size: 980 860 50 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 45 75 226 256 1890 Size: 45 30 151 30 1634 Fragments arranged by size: 1634 151 45 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 69 394 1890 Size: 69 325 1496 Fragments arranged by size: 1496 325 69 BseYI C'CCAG_C Cuts at: 0 823 1890 Size: 823 1067 BsmI GAATG_Cn' Cuts at: 0 1306 1890 Size: 1306 584 Bsp1286I G_dGCh'C Cuts at: 0 116 1890 Size: 116 1774 BspHI T'CATG_A Cuts at: 0 1884 1890 Size: 1884 6 BsrDI GCAATG_nn' Cuts at: 0 1311 1890 Size: 1311 579 BsrGI T'GTAC_A Cuts at: 0 1011 1683 1890 Size: 1011 672 207 Fragments arranged by size: 1011 672 207 BstYI r'GATC_y Cuts at: 0 147 1189 1890 Size: 147 1042 701 Fragments arranged by size: 1042 701 147 Bsu36I CC'TnA_GG Cuts at: 0 406 1890 Size: 406 1484 BtgI C'CryG_G Cuts at: 0 44 128 268 1071 1890 Size: 44 84 140 803 819 Fragments arranged by size: 819 803 140 84 44 BtsI GCAGTG_nn' Cuts at: 0 459 1890 Size: 459 1431 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1293 1328 1890 Size: 1293 35 562 Fragments arranged by size: 1293 562 35 DraI TTT'AAA Cuts at: 0 1578 1890 Size: 1578 312 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 115 205 821 1136 1140 1640 1808 1890 Size: 115 90 616 315 4 500 168 82 Fragments arranged by size: 616 500 315 168 115 90 82 4 EcoRI G'AATT_C Cuts at: 0 1106 1358 1890 Size: 1106 252 532 Fragments arranged by size: 1106 532 252 HaeII r_GCGC'y Cuts at: 0 128 1890 Size: 128 1762 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 226 258 682 714 1890 Size: 226 32 424 32 1176 Fragments arranged by size: 1176 424 226 32 32 HindIII A'AGCT_T Cuts at: 0 860 1403 1890 Size: 860 543 487 Fragments arranged by size: 860 543 487 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 230 1890 Size: 230 1660 MslI CAynn'nnrTG Cuts at: 0 963 1047 1082 1199 1215 1283 1299 1367 Size: 963 84 35 117 16 68 16 68 Cuts at: 1367 1535 1890 Size: 168 355 Fragments arranged by size: 963 355 168 117 84 68 68 35 16 16 MspA1I CmG'CkG Cuts at: 0 14 130 1890 Size: 14 116 1760 Fragments arranged by size: 1760 116 14 NcoI C'CATG_G Cuts at: 0 268 1071 1890 Size: 268 803 819 Fragments arranged by size: 819 803 268 NdeI CA'TA_TG Cuts at: 0 960 1044 1212 1296 1520 1890 Size: 960 84 168 84 224 370 Fragments arranged by size: 960 370 224 168 84 84 NsiI A_TGCA'T Cuts at: 0 1842 1890 Size: 1842 48 NspI r_CATG'y Cuts at: 0 502 1087 1186 1270 1858 1890 Size: 502 585 99 84 588 32 Fragments arranged by size: 588 585 502 99 84 32 PciI A'CATG_T Cuts at: 0 498 1182 1266 1854 1890 Size: 498 684 84 588 36 Fragments arranged by size: 684 588 498 84 36 PsiI TTA'TAA Cuts at: 0 1466 1890 Size: 1466 424 SacII CC_GC'GG Cuts at: 0 131 1890 Size: 131 1759 SmlI C'TyrA_G Cuts at: 0 434 579 857 1890 Size: 434 145 278 1033 Fragments arranged by size: 1033 434 278 145 StuI AGG'CCT Cuts at: 0 1592 1890 Size: 1592 298 StyI C'CwwG_G Cuts at: 0 268 1071 1890 Size: 268 803 819 Fragments arranged by size: 819 803 268 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 837 1890 Size: 837 1053 TatI w'GTAC_w Cuts at: 0 1011 1683 1890 Size: 1011 672 207 Fragments arranged by size: 1011 672 207 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 45 77 1890 Size: 45 32 1813 Fragments arranged by size: 1813 45 32 Tth111I GACn'n_nGTC Cuts at: 0 293 1890 Size: 293 1597 XmnI GAAnn'nnTTC Cuts at: 0 631 1052 1153 1405 1825 1890 Size: 631 421 101 252 420 65 Fragments arranged by size: 631 421 420 252 101 65 Enzymes that do cut: AcuI AflIII AleI AlwNI ApoI AvaI BamHI BanII BclI BfrBI BglII BmrI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI BsmI Bsp1286I BspHI BsrDI BsrGI BstYI Bsu36I BtgI BtsI CspCI DraI Eco57MI EcoRI HaeII Hin4I HindIII MmeI MslI MspA1I NcoI NdeI NsiI NspI PciI PsiI SacII SmlI StuI StyI TaqII TatI TstI Tth111I XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BanI BbeI BbsI BbvCI BcgI BciVI BglI BlpI Bme1580I BmgBI BmtI BplI BsaAI BsaBI BsaHI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI ClaI DraIII DrdI EaeI EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HincII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NgoMIV NheI NotI NruI PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI XbaI XcmI XhoI XmaI ZraI