################################## # NO CUT ENZYMES ( 112 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BbeI BbrPI BcgI BfrI BglI BlnI BpmI BsaAI BseAI BsiWI BsmBI BsmI BspDI BspEI BssHII Bst1107I BstAPI BstBI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KasI KpnI MfeI MluI MroI MseI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI Tru9I Tth111I XcmI XmnI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AccII 23 CGCG CG^CG AcyI 135 GGCGTC G[GA]^CG[CT]C AlwNI 610 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 715 GAGCTC G[AT]GC[AT]^C BamHI 768 GGATCC G^GATCC BanI 803 GGCACC G^G[CT][GA]CC BbsI 1018 GTCTTC GAAGAC(2/6) BbvCI 924 GCTGAGG CCTCAGC(-5/-2) BciVI 729 GGATAC GTATCC(6/5) BfaI 196 CTAG C^TAG BlpI 294 GCTCAGC GC^T[ACGT]AGC Bpu1102I 294 GCTCAGC GC^T[ACGT]AGC BpuAI 1018 GTCTTC GAAGAC(2/6) BsaHI 135 GGCGTC G[GA]^CG[CT]C BsaI 1065 GGTCTC GGTCTC(1/5) BsaWI 148 ACCGGA [AT]^CCGG[AT] BsgI 952 GTGCAG GTGCAG(16/14) BsiEI 300 CGACCG CG[GA][CT]^CG BsiHKAI 715 GAGCTC G[AT]GC[AT]^C Bsp1407I 1274 TGTACA T^GTACA BspHI 1052 TCATGA T^CATGA BsrBI 504 CCGCTC CCGCTC(-3/-3) BsrDI 846 CATTGC GCAATG(2/0) BsrFI 1252 ACCGGC [GA]^CCGG[CT] BsrGI 1274 TGTACA T^GTACA BssSI 124 CTCGTG CACGAG(-5/-1) BstEII 12 GGTCACC G^GT[ACGT]ACC BstPI 12 GGTCACC G^GT[ACGT]ACC BstUI 23 CGCG CG^CG BstXI 998 CCAGCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 294 GCTCAGC GC^T[ACGT]AGC Cfr10I 1252 ACCGGC [GA]^CCGG[CT] EarI 580 CTCTTC CTCTTC(1/4) EcoO65I 12 GGTCACC G^GT[ACGT]ACC HgaI 136 GCGTC GACGC(5/10) Hin1I 135 GGCGTC G[GA]^CG[CT]C HinfI 691 GAGTC G^A[ACGT]TC Ksp632I 580 CTCTTC CTCTTC(1/4) KspI 22 CCGCGG CCGC^GG MaeI 196 CTAG C^TAG MaeII 905 ACGT A^CGT MvnI 23 CGCG CG^CG NcoI 1298 CCATGG C^CATGG NdeI 1403 CATATG CA^TATG PaeR7I 1130 CTCGAG C^TCGAG PflMI 811 CCATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 691 GAGTC GAGTC(4/5) PvuII 1234 CAGCTG CAG^CTG RcaI 1052 TCATGA T^CATGA SacI 715 GAGCTC GAGCT^C SacII 22 CCGCGG CCGC^GG SmaI 185 CCCGGG CCC^GGG SspBI 1274 TGTACA T^GTACA TaqI 1131 TCGA T^CGA TthHB8I 1131 TCGA T^CGA Van91I 811 CCATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 195 TCTAGA T^CTAGA XhoI 1130 CTCGAG C^TCGAG XmaCI 185 CCCGGG C^CCGGG XmaI 185 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AflIII 1115 ACATGT A^C[GA][CT]GT 1304 ACATGT BclI 774 TGATCA T^GATCA 1049 TGATCA BglII 317 AGATCT A^GATCT 1283 AGATCT BsaBI 318 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 769 GATCCTGATC BspLU11I 1115 ACATGT A^CATGT 1304 ACATGT BsrI 389 ACTGG ACTGG(1/-1) 394 CCAGT EcoT14I 1241 CCTTGG C^C[AT][AT]GG 1298 CCATGG FbaI 774 TGATCA T^GATCA 1049 TGATCA HaeII 420 GGCGCT [GA]GCGC^[CT] 471 GGCGCT MamI 318 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 769 GATCCTGATC PpuMI 55 AGGACCT [GA]G^G[AT]CC[CT] 683 AGGTCCC SmlI 373 CTCAAG C^T[CT][GA]AG 1130 CTCGAG StyI 1241 CCTTGG C^C[AT][AT]GG 1298 CCATGG Tsp509I 226 AATT ^AATT 1365 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AciI AfaI AluI AlwI AvaI AvaII BalI BanII BbvI BcnI BmyI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TseI Tsp45I TspRI XhoII