(Circular) (Six-base) MAPSORT of: pFN21AE6461.seq Check: 6125 from: 1 to: 6130 pFN21AE6461 6130 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2030 2049 2950 2030 Size: 19 901 5210 Fragments arranged by size: 5210 901 19 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3940 278 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3473 1937 Size: 1536 4594 Acc65I G'GTAC_C Cuts at: 3451 3451 Size: 6130 AclI AA'CG_TT Cuts at: 1563 4257 4630 1563 Size: 2694 373 3063 Fragments arranged by size: 3063 2694 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4193 5577 5845 1632 Size: 2561 1384 268 1917 Fragments arranged by size: 2561 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5933 AflIII A'CryG_T Cuts at: 1203 3120 3309 5029 6006 1203 Size: 1917 189 1720 977 1327 Fragments arranged by size: 1917 1720 1327 977 189 AgeI A'CCGG_T Cuts at: 4961 4961 Size: 6130 AhdI GACnn_n'nnGTC Cuts at: 4859 4859 Size: 6130 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6130 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2357 1184 Size: 1173 4957 AlwNI CAG_nnn'CTG Cuts at: 2620 3873 5445 2620 Size: 1253 1572 3305 Fragments arranged by size: 3305 1572 1253 ApaLI G'TGCA_C Cuts at: 4187 5343 4187 Size: 1156 4974 ApoI r'AATT_y Cuts at: 1493 3439 3665 3817 5926 1493 Size: 1946 226 152 2109 1697 Fragments arranged by size: 2109 1946 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4684 160 Size: 872 3652 1606 Fragments arranged by size: 3652 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 AvaI C'yCGr_G Cuts at: 1870 1943 2012 2056 2190 2366 3135 3455 1870 Size: 73 69 44 134 176 769 320 4545 Fragments arranged by size: 4545 769 320 176 134 73 69 44 BamHI G'GATC_C Cuts at: 2773 3460 3827 2773 Size: 687 367 5076 Fragments arranged by size: 5076 687 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2808 3451 4906 618 Size: 325 217 627 1021 643 1455 1842 Fragments arranged by size: 1842 1455 1021 643 627 325 217 BanII G_rGCy'C Cuts at: 1391 2012 2076 2200 2495 2724 3091 3209 3323 Size: 621 64 124 295 229 367 118 114 Cuts at: 3323 3449 1391 Size: 126 4072 Fragments arranged by size: 4072 621 367 295 229 126 124 118 114 64 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3016 928 Size: 971 1117 4042 Fragments arranged by size: 4042 1117 971 BbvCI CC'TCA_GC Cuts at: 2930 2930 Size: 6130 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4321 4355 1903 Size: 34 2384 34 3678 Fragments arranged by size: 3678 2384 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2728 4022 5232 2728 Size: 1294 1210 3626 Fragments arranged by size: 3626 1294 1210 BclI T'GATC_A Cuts at: 1548 2779 3054 1548 Size: 1231 275 4624 Fragments arranged by size: 4624 1231 275 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4741 136 Size: 107 122 71 631 605 3069 1525 Fragments arranged by size: 3069 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2322 3288 6125 2322 Size: 966 2837 2327 Fragments arranged by size: 2837 2327 966 BlpI GC'TnA_GC Cuts at: 2300 3547 2300 Size: 1247 4883 Bme1580I G_kGCm'C Cuts at: 1790 2607 2811 2903 4191 5347 1790 Size: 817 204 92 1288 1156 2573 Fragments arranged by size: 2573 1288 1156 817 204 92 BmgBI CAC'GTC Cuts at: 1318 2911 1318 Size: 1593 4537 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3861 4819 442 Size: 416 53 83 399 24 2444 958 1753 Fragments arranged by size: 2444 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6130 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2955 3110 2955 Size: 155 5975 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4790 1239 Size: 3551 2579 Bpu10I CC'TnA_GC Cuts at: 2930 2930 Size: 6130 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2363 4255 5120 5418 2363 Size: 1892 865 298 3075 Fragments arranged by size: 3075 1892 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3076 4793 882 Size: 617 1577 1717 2219 Fragments arranged by size: 2219 1717 1577 617 BsaAI yAC'GTr Cuts at: 493 1204 3921 493 Size: 711 2717 2702 Fragments arranged by size: 2717 2702 711 BsaBI GATnn'nnATC Cuts at: 2327 2778 6124 2327 Size: 451 3346 2333 Fragments arranged by size: 3346 2333 451 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2141 3937 4319 4954 Size: 53 83 186 936 608 1796 382 635 Cuts at: 4954 275 Size: 1451 Fragments arranged by size: 1796 1451 936 635 608 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2153 4563 4961 5235 5382 5793 2153 Size: 2410 398 274 147 411 2490 Fragments arranged by size: 2490 2410 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2396 2426 1178 Size: 30 165 30 993 30 4882 Fragments arranged by size: 4882 993 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2117 2152 2485 3083 3199 3328 1186 Size: 931 35 333 598 116 129 3988 Fragments arranged by size: 3988 931 598 333 129 116 35 BseYI C'CCAG_C Cuts at: 2290 2344 2479 2488 2542 2600 2711 5333 5829 Size: 54 135 9 54 58 111 2622 496 Cuts at: 5829 2290 Size: 2591 Fragments arranged by size: 2622 2591 496 135 111 58 54 54 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2978 1163 Size: 573 1242 4315 Fragments arranged by size: 4315 1242 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2308 3580 4341 5369 1540 Size: 275 186 307 1272 761 1028 2301 Fragments arranged by size: 2301 1272 1028 761 307 275 186 BsiHKAI G_wGCw'C Cuts at: 2724 4191 4276 5347 2724 Size: 1467 85 1071 3507 Fragments arranged by size: 3507 1467 1071 85 BsmI GAATG_Cn' Cuts at: 3641 3734 3641 Size: 93 6037 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2076 2200 2495 2607 2724 2811 Size: 399 222 64 124 295 112 117 87 Cuts at: 2811 2903 3091 3209 3323 3449 4191 4276 5347 Size: 92 188 118 114 126 742 85 1071 Cuts at: 5347 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 295 222 188 126 124 118 117 114 112 92 87 85 64 BspEI T'CCGG_A Cuts at: 5793 5793 Size: 6130 BspHI T'CATG_A Cuts at: 3057 4019 5749 3057 Size: 962 1730 3438 Fragments arranged by size: 3438 1730 962 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2030 2049 2950 3485 844 Size: 1186 19 901 535 3489 Fragments arranged by size: 3489 1186 901 535 19 BsrBI CCG'CTC Cuts at: 1394 1823 2511 4017 1394 Size: 429 688 1506 3507 Fragments arranged by size: 3507 1506 688 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2850 4625 4799 65 Size: 1183 1602 1775 174 1396 Fragments arranged by size: 1775 1602 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3257 4774 4961 1700 Size: 110 1447 1517 187 2869 Fragments arranged by size: 2869 1517 1447 187 110 BsrGI T'GTAC_A Cuts at: 96 3279 96 Size: 3183 2947 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6130 BssSI C'ACGA_G Cuts at: 2129 4190 5202 2129 Size: 2061 1012 3057 Fragments arranged by size: 3057 2061 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6130 BstBI TT'CG_AA Cuts at: 4934 4934 Size: 6130 BstEII G'GTnAC_C Cuts at: 1184 2017 1184 Size: 833 5297 BstXI CCAn_nnnn'nTGG Cuts at: 3010 3010 Size: 6130 BstYI r'GATC_y Cuts at: 1972 2322 2773 3288 3460 3827 4212 4229 5681 Size: 350 451 515 172 367 385 17 1452 Cuts at: 5681 5767 5779 6125 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 515 451 385 367 350 346 172 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2027 2730 3303 513 Size: 552 962 703 573 3340 Fragments arranged by size: 3340 962 703 573 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2169 487 Size: 683 56 943 4448 Fragments arranged by size: 4448 943 683 56 BtsI GCAGTG_nn' Cuts at: 2808 3646 4440 4460 2808 Size: 838 794 20 4478 Fragments arranged by size: 4478 838 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6130 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6095 DraI TTT'AAA Cuts at: 3434 3434 Size: 6130 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5137 809 Size: 507 3821 1802 Fragments arranged by size: 3821 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2396 2703 3357 Size: 54 1425 50 275 18 566 307 654 Cuts at: 3357 3577 4466 6095 8 Size: 220 889 1629 43 Fragments arranged by size: 1629 1425 889 654 566 307 275 220 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3577 1537 Size: 275 1765 4090 Fragments arranged by size: 4090 1765 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2591 4060 1353 Size: 1238 1469 3423 Fragments arranged by size: 3423 1469 1238 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2269 2443 4717 5091 5237 387 Size: 1064 138 680 174 2274 374 146 1280 Fragments arranged by size: 2274 1280 1064 680 374 174 146 138 EcoICRI GAG'CTC Cuts at: 2722 2722 Size: 6130 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4193 4790 5577 5845 1239 Size: 393 2561 597 787 268 1524 Fragments arranged by size: 2561 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2061 2617 2689 2061 Size: 556 72 5502 Fragments arranged by size: 5502 556 72 EcoRI G'AATT_C Cuts at: 3439 3817 3439 Size: 378 5752 FspI TGC'GCA Cuts at: 4636 4636 Size: 6130 HaeII r_GCGC'y Cuts at: 1678 2429 2480 5277 1678 Size: 751 51 2797 2531 Fragments arranged by size: 2797 2531 751 51 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2726 2758 4771 4803 4845 Size: 32 135 32 1154 32 2013 32 42 Cuts at: 4845 4877 1373 Size: 32 2626 Fragments arranged by size: 2626 2013 1154 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3474 3720 669 Size: 988 281 1536 246 3079 Fragments arranged by size: 3079 1536 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6130 HpaI GTT'AAC Cuts at: 3720 3720 Size: 6130 KpnI G_GTAC'C Cuts at: 3455 3455 Size: 6130 MfeI C'AATT_G Cuts at: 3729 3729 Size: 6130 MluI A'CGCG_T Cuts at: 6006 6006 Size: 6130 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2190 2766 2927 5244 5428 1305 Size: 885 576 161 2317 184 2007 Fragments arranged by size: 2317 2007 885 576 184 161 MscI TGG'CCA Cuts at: 10 64 1489 1832 2398 2705 3359 10 Size: 54 1425 343 566 307 654 2781 Fragments arranged by size: 2781 1425 654 566 343 307 54 MslI CAynn'nnrTG Cuts at: 518 1166 2333 3050 3056 3308 3314 4088 4447 Size: 648 1167 717 6 252 6 774 359 Cuts at: 4447 4606 518 Size: 159 2042 Fragments arranged by size: 2042 1167 774 717 648 359 252 159 6 6 MspA1I CmG'CkG Cuts at: 1606 2029 3033 3241 3546 4223 5371 5616 1606 Size: 423 1004 208 305 677 1148 245 2120 Fragments arranged by size: 2120 1148 1004 677 423 305 245 208 NcoI C'CATG_G Cuts at: 513 1065 3303 513 Size: 552 2238 3340 Fragments arranged by size: 3340 2238 552 NdeI CA'TA_TG Cuts at: 387 3409 387 Size: 3022 3108 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6130 NotI GC'GGCC_GC Cuts at: 3577 3577 Size: 6130 NspI r_CATG'y Cuts at: 1132 1229 2338 3124 3313 3488 5033 1132 Size: 97 1109 786 189 175 1545 2229 Fragments arranged by size: 2229 1545 1109 786 189 175 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6130 PciI A'CATG_T Cuts at: 3120 3309 5029 3120 Size: 189 1720 4221 Fragments arranged by size: 4221 1720 189 PflMI CCAn_nnn'nTGG Cuts at: 1108 2822 1108 Size: 1714 4416 PfoI T'CCnGG_A Cuts at: 2821 2821 Size: 6130 PmeI GTTT'AAAC Cuts at: 3434 3434 Size: 6130 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4199 4231 5712 5744 4199 Size: 32 1481 32 4585 Fragments arranged by size: 4585 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2061 2689 2061 Size: 628 5502 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6130 PsiI TTA'TAA Cuts at: 3700 3700 Size: 6130 PspXI vC'TCGA_Gb Cuts at: 3135 3135 Size: 6130 PstI C_TGCA'G Cuts at: 830 2639 2983 3240 3482 830 Size: 1809 344 257 242 3478 Fragments arranged by size: 3478 1809 344 257 242 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6130 PvuII CAG'CTG Cuts at: 3241 3241 Size: 6130 SacI G_AGCT'C Cuts at: 2724 2724 Size: 6130 SacII CC_GC'GG Cuts at: 2030 2030 Size: 6130 SalI G'TCGA_C Cuts at: 1936 3472 1936 Size: 1536 4594 SbfI CC_TGCA'GG Cuts at: 3482 3482 Size: 6130 ScaI AGT'ACT Cuts at: 4378 6090 4378 Size: 1712 4418 SfcI C'TryA_G Cuts at: 826 1045 2635 2668 2979 3236 3478 4613 5294 Size: 219 1590 33 311 257 242 1135 681 Cuts at: 5294 5485 826 Size: 191 1471 Fragments arranged by size: 1590 1471 1135 681 311 257 242 219 191 33 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6130 SmaI CCC'GGG Cuts at: 1872 2192 3457 1872 Size: 320 1265 4545 Fragments arranged by size: 4545 1265 320 SmlI C'TyrA_G Cuts at: 820 1017 1943 2378 3135 4234 5135 5397 820 Size: 197 926 435 757 1099 901 262 1553 Fragments arranged by size: 1553 1099 926 901 757 435 262 197 SnaBI TAC'GTA Cuts at: 493 3921 493 Size: 3428 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6130 SphI G_CATG'C Cuts at: 1132 3488 1132 Size: 2356 3774 SspI AAT'ATT Cuts at: 5 52 4054 5 Size: 47 4002 2081 Fragments arranged by size: 4002 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6130 StyI C'CwwG_G Cuts at: 513 1065 3246 3303 3569 513 Size: 552 2181 57 266 3074 Fragments arranged by size: 3074 2181 552 266 57 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2858 3002 4157 4174 4327 4512 4957 Size: 1255 1278 144 1155 17 153 185 445 Cuts at: 4957 325 Size: 1498 Fragments arranged by size: 1498 1278 1255 1155 445 185 153 144 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3279 4376 6088 Size: 274 80 33 51 1443 1302 1097 1712 Cuts at: 6088 96 Size: 138 Fragments arranged by size: 1712 1443 1302 1097 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2758 2790 6090 6122 2758 Size: 32 3300 32 2766 Fragments arranged by size: 3300 2766 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6130 XbaI T'CTAG_A Cuts at: 2200 3466 2200 Size: 1266 4864 XhoI C'TCGA_G Cuts at: 1943 3135 1943 Size: 1192 4938 XmaI C'CCGG_G Cuts at: 1870 2190 3455 1870 Size: 320 1265 4545 Fragments arranged by size: 4545 1265 320 XmnI GAAnn'nnTTC Cuts at: 4259 4259 Size: 6130 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3938 276 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI