(Linear) (Six-base) MAPSORT of: KIEE6461.seq Check: 8757 from: 1 to: 1425 KIEE6461.orf With 182 enzymes: SgfI * March 16, 2015 11:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 25 44 945 1425 Size: 25 19 901 480 Fragments arranged by size: 901 480 25 19 AflIII A'CryG_T Cuts at: 0 1115 1304 1425 Size: 1115 189 121 Fragments arranged by size: 1115 189 121 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 352 1425 Size: 352 1073 AlwNI CAG_nnn'CTG Cuts at: 0 615 1425 Size: 615 810 AvaI C'yCGr_G Cuts at: 0 7 51 185 361 1130 1425 Size: 7 44 134 176 769 295 Fragments arranged by size: 769 295 176 134 44 7 BamHI G'GATC_C Cuts at: 0 768 1425 Size: 768 657 BanI G'GyrC_C Cuts at: 0 803 1425 Size: 803 622 BanII G_rGCy'C Cuts at: 0 7 71 195 490 719 1086 1204 1318 Size: 7 64 124 295 229 367 118 114 Cuts at: 1318 1425 Size: 107 Fragments arranged by size: 367 295 229 124 118 114 107 64 7 BbsI GAAGACnn'nnnn_ Cuts at: 0 1011 1425 Size: 1011 414 BbvCI CC'TCA_GC Cuts at: 0 925 1425 Size: 925 500 BciVI GTATCCnnnnn_n' Cuts at: 0 723 1425 Size: 723 702 BclI T'GATC_A Cuts at: 0 774 1049 1425 Size: 774 275 376 Fragments arranged by size: 774 376 275 BglII A'GATC_T Cuts at: 0 317 1283 1425 Size: 317 966 142 Fragments arranged by size: 966 317 142 BlpI GC'TnA_GC Cuts at: 0 295 1425 Size: 295 1130 Bme1580I G_kGCm'C Cuts at: 0 602 806 898 1425 Size: 602 204 92 527 Fragments arranged by size: 602 527 204 92 BmgBI CAC'GTC Cuts at: 0 906 1425 Size: 906 519 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 950 1105 1425 Size: 950 155 320 Fragments arranged by size: 950 320 155 Bpu10I CC'TnA_GC Cuts at: 0 925 1425 Size: 925 500 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 358 1425 Size: 358 1067 BsaI GGTCTCn'nnnn_ Cuts at: 0 1071 1425 Size: 1071 354 BsaBI GATnn'nnATC Cuts at: 0 322 773 1425 Size: 322 451 652 Fragments arranged by size: 652 451 322 BsaHI Gr'CG_yC Cuts at: 0 136 1425 Size: 136 1289 BsaWI w'CCGG_w Cuts at: 0 148 1425 Size: 148 1277 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 391 421 1425 Size: 391 30 1004 Fragments arranged by size: 1004 391 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 112 147 480 1078 1194 1323 1425 Size: 112 35 333 598 116 129 102 Fragments arranged by size: 598 333 129 116 112 102 35 BseYI C'CCAG_C Cuts at: 0 285 339 474 483 537 595 706 1425 Size: 285 54 135 9 54 58 111 719 Fragments arranged by size: 719 285 135 111 58 54 54 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 973 1425 Size: 973 452 BsiEI CG_ry'CG Cuts at: 0 303 1425 Size: 303 1122 BsiHKAI G_wGCw'C Cuts at: 0 719 1425 Size: 719 706 Bsp1286I G_dGCh'C Cuts at: 0 7 71 195 490 602 719 806 898 Size: 7 64 124 295 112 117 87 92 Cuts at: 898 1086 1204 1318 1425 Size: 188 118 114 107 Fragments arranged by size: 295 188 124 118 117 114 112 107 92 87 64 7 BspHI T'CATG_A Cuts at: 0 1052 1425 Size: 1052 373 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 25 44 945 1425 Size: 25 19 901 480 Fragments arranged by size: 901 480 25 19 BsrBI CCG'CTC Cuts at: 0 506 1425 Size: 506 919 BsrDI GCAATG_nn' Cuts at: 0 845 1425 Size: 845 580 BsrFI r'CCGG_y Cuts at: 0 1252 1425 Size: 1252 173 BsrGI T'GTAC_A Cuts at: 0 1274 1425 Size: 1274 151 BssSI C'ACGA_G Cuts at: 0 124 1425 Size: 124 1301 BstEII G'GTnAC_C Cuts at: 0 12 1425 Size: 12 1413 BstXI CCAn_nnnn'nTGG Cuts at: 0 1005 1425 Size: 1005 420 BstYI r'GATC_y Cuts at: 0 317 768 1283 1425 Size: 317 451 515 142 Fragments arranged by size: 515 451 317 142 BtgI C'CryG_G Cuts at: 0 22 725 1298 1425 Size: 22 703 573 127 Fragments arranged by size: 703 573 127 22 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 164 1425 Size: 164 1261 BtsI GCAGTG_nn' Cuts at: 0 803 1425 Size: 803 622 EaeI y'GGCC_r Cuts at: 0 391 698 1352 1425 Size: 391 307 654 73 Fragments arranged by size: 654 391 307 73 EarI CTCTTCn'nnn_ Cuts at: 0 586 1425 Size: 586 839 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 264 438 1425 Size: 264 174 987 Fragments arranged by size: 987 264 174 EcoICRI GAG'CTC Cuts at: 0 717 1425 Size: 717 708 EcoO109I rG'GnC_Cy Cuts at: 0 56 612 684 1425 Size: 56 556 72 741 Fragments arranged by size: 741 556 72 56 HaeII r_GCGC'y Cuts at: 0 424 475 1425 Size: 424 51 950 Fragments arranged by size: 950 424 51 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 721 753 1425 Size: 721 32 672 Fragments arranged by size: 721 672 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 185 761 922 1425 Size: 185 576 161 503 Fragments arranged by size: 576 503 185 161 MscI TGG'CCA Cuts at: 0 393 700 1354 1425 Size: 393 307 654 71 Fragments arranged by size: 654 393 307 71 MslI CAynn'nnrTG Cuts at: 0 328 1045 1051 1303 1309 1425 Size: 328 717 6 252 6 116 Fragments arranged by size: 717 328 252 116 6 6 MspA1I CmG'CkG Cuts at: 0 24 1028 1236 1425 Size: 24 1004 208 189 Fragments arranged by size: 1004 208 189 24 NcoI C'CATG_G Cuts at: 0 1298 1425 Size: 1298 127 NdeI CA'TA_TG Cuts at: 0 1404 1425 Size: 1404 21 NspI r_CATG'y Cuts at: 0 333 1119 1308 1425 Size: 333 786 189 117 Fragments arranged by size: 786 333 189 117 PciI A'CATG_T Cuts at: 0 1115 1304 1425 Size: 1115 189 121 Fragments arranged by size: 1115 189 121 PflMI CCAn_nnn'nTGG Cuts at: 0 817 1425 Size: 817 608 PfoI T'CCnGG_A Cuts at: 0 816 1425 Size: 816 609 PpuMI rG'GwC_Cy Cuts at: 0 56 684 1425 Size: 56 628 741 Fragments arranged by size: 741 628 56 PspXI vC'TCGA_Gb Cuts at: 0 1130 1425 Size: 1130 295 PstI C_TGCA'G Cuts at: 0 634 978 1235 1425 Size: 634 344 257 190 Fragments arranged by size: 634 344 257 190 PvuII CAG'CTG Cuts at: 0 1236 1425 Size: 1236 189 SacI G_AGCT'C Cuts at: 0 719 1425 Size: 719 706 SacII CC_GC'GG Cuts at: 0 25 1425 Size: 25 1400 SfcI C'TryA_G Cuts at: 0 630 663 974 1231 1425 Size: 630 33 311 257 194 Fragments arranged by size: 630 311 257 194 33 SmaI CCC'GGG Cuts at: 0 187 1425 Size: 187 1238 SmlI C'TyrA_G Cuts at: 0 373 1130 1425 Size: 373 757 295 Fragments arranged by size: 757 373 295 StyI C'CwwG_G Cuts at: 0 1241 1298 1425 Size: 1241 57 127 Fragments arranged by size: 1241 127 57 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 853 997 1425 Size: 853 144 428 Fragments arranged by size: 853 428 144 TatI w'GTAC_w Cuts at: 0 1274 1425 Size: 1274 151 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 753 785 1425 Size: 753 32 640 Fragments arranged by size: 753 640 32 XbaI T'CTAG_A Cuts at: 0 195 1425 Size: 195 1230 XhoI C'TCGA_G Cuts at: 0 1130 1425 Size: 1130 295 XmaI C'CCGG_G Cuts at: 0 185 1425 Size: 185 1240 Enzymes that do cut: AarI AflIII AlfI AlwNI AvaI BamHI BanI BanII BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmgBI BplI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstEII BstXI BstYI BtgI BtgZI BtsI EaeI EarI EciI EcoICRI EcoO109I HaeII Hin4I MmeI MscI MslI MspA1I NcoI NdeI NspI PciI PflMI PfoI PpuMI PspXI PstI PvuII SacI SacII SfcI SmaI SmlI StyI TaqII TatI TstI XbaI XhoI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI BcgI BfrBI BglI BmrI BmtI BpmI BsaAI BsiWI BsmI BsmBI BspEI BssHII BstAPI BstBI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PasI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XcmI XmnI ZraI