(Circular) (Six-base) MAPSORT of: pFN21AE5445.seq Check: 7393 from: 1 to: 5653 pFN21AE5445 5653 bp With 182 enzymes: SgfI * January 30, 2014 17:20 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3463 278 Size: 53 83 186 936 1927 2468 Fragments arranged by size: 2468 1927 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2996 1937 Size: 1059 4594 Acc65I G'GTAC_C Cuts at: 2974 2974 Size: 5653 AclI AA'CG_TT Cuts at: 1563 3780 4153 1563 Size: 2217 373 3063 Fragments arranged by size: 3063 2217 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2077 3716 5100 5368 1632 Size: 445 1639 1384 268 1917 Fragments arranged by size: 1917 1639 1384 445 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5456 AflIII A'CryG_T Cuts at: 1203 2534 4552 5529 1203 Size: 1331 2018 977 1327 Fragments arranged by size: 2018 1331 1327 977 AgeI A'CCGG_T Cuts at: 4484 4484 Size: 5653 AhdI GACnn_n'nnGTC Cuts at: 4382 4382 Size: 5653 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5653 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5653 AlwNI CAG_nnn'CTG Cuts at: 3396 4968 3396 Size: 1572 4081 ApaLI G'TGCA_C Cuts at: 2427 3710 4866 2427 Size: 1283 1156 3214 Fragments arranged by size: 3214 1283 1156 ApoI r'AATT_y Cuts at: 1493 2541 2962 3188 3340 5449 1493 Size: 1048 421 226 152 2109 1697 Fragments arranged by size: 2109 1697 1048 421 226 152 AseI AT'TA_AT Cuts at: 160 1032 4207 160 Size: 872 3175 1606 Fragments arranged by size: 3175 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 AvaI C'yCGr_G Cuts at: 1870 1943 2978 1870 Size: 73 1035 4545 Fragments arranged by size: 4545 1035 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2291 2324 2291 Size: 33 5620 BamHI G'GATC_C Cuts at: 2983 3350 2983 Size: 367 5286 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2726 2974 4429 618 Size: 325 217 627 939 248 1455 1842 Fragments arranged by size: 1842 1455 939 627 325 248 217 BanII G_rGCy'C Cuts at: 1391 2330 2972 1391 Size: 939 642 4072 Fragments arranged by size: 4072 939 642 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2083 928 Size: 971 184 4498 Fragments arranged by size: 4498 971 184 BbvCI CC'TCA_GC Cuts at: 2689 2689 Size: 5653 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3844 3878 1903 Size: 34 50 34 1823 34 3678 Fragments arranged by size: 3678 1823 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3545 4755 3545 Size: 1210 4443 BclI T'GATC_A Cuts at: 1548 2145 1548 Size: 597 5056 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2357 4264 136 Size: 107 122 71 631 605 685 1907 1525 Fragments arranged by size: 1907 1525 685 631 605 122 107 71 BglII A'GATC_T Cuts at: 5648 5648 Size: 5653 BlpI GC'TnA_GC Cuts at: 3070 3070 Size: 5653 Bme1580I G_kGCm'C Cuts at: 1790 2431 2731 3714 4870 1790 Size: 641 300 983 1156 2573 Fragments arranged by size: 2573 1156 983 641 300 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5653 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2053 3384 4342 Size: 416 53 83 399 24 636 1331 958 Cuts at: 4342 442 Size: 1753 Fragments arranged by size: 1753 1331 958 636 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5653 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4313 1239 Size: 3074 2579 Bpu10I CC'TnA_GC Cuts at: 2689 2689 Size: 5653 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3778 4643 4941 3778 Size: 865 298 4490 Fragments arranged by size: 4490 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2449 4316 882 Size: 617 950 1867 2219 Fragments arranged by size: 2219 1867 950 617 BsaAI yAC'GTr Cuts at: 493 1204 2535 3444 493 Size: 711 1331 909 2702 Fragments arranged by size: 2702 1331 909 711 BsaBI GATnn'nnATC Cuts at: 2150 5647 2150 Size: 3497 2156 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3460 3842 4477 275 Size: 53 83 186 936 1927 382 635 1451 Fragments arranged by size: 1927 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4086 4484 4758 4905 5316 4086 Size: 398 274 147 411 4423 Fragments arranged by size: 4423 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5428 Fragments arranged by size: 5428 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2606 1186 Size: 1420 4233 BseYI C'CCAG_C Cuts at: 2914 4856 5352 2914 Size: 1942 496 3215 Fragments arranged by size: 3215 1942 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5080 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3103 3864 4892 1540 Size: 275 186 1102 761 1028 2301 Fragments arranged by size: 2301 1102 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2346 2431 3714 3799 4870 2346 Size: 85 1283 85 1071 3129 Fragments arranged by size: 3129 1283 1071 85 85 BsmI GAATG_Cn' Cuts at: 2610 3164 3257 2610 Size: 554 93 5006 Fragments arranged by size: 5006 554 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2330 2346 2431 2731 2972 3714 3799 Size: 399 540 16 85 300 241 742 85 Cuts at: 3799 4870 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 540 399 300 241 85 85 16 BspEI T'CCGG_A Cuts at: 5316 5316 Size: 5653 BspHI T'CATG_A Cuts at: 2409 2583 3542 5272 2409 Size: 174 959 1730 2790 Fragments arranged by size: 2790 1730 959 174 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3008 844 Size: 2164 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2471 3540 1394 Size: 429 648 1069 3507 Fragments arranged by size: 3507 1069 648 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2927 4148 4322 65 Size: 1183 1679 1221 174 1396 Fragments arranged by size: 1679 1396 1221 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4297 4484 1700 Size: 110 2487 187 2869 Fragments arranged by size: 2869 2487 187 110 BsrGI T'GTAC_A Cuts at: 96 2299 96 Size: 2203 3450 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5653 BssSI C'ACGA_G Cuts at: 3713 4725 3713 Size: 1012 4641 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5653 BstBI TT'CG_AA Cuts at: 4457 4457 Size: 5653 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5653 BstXI CCAn_nnnn'nTGG Cuts at: 2599 2599 Size: 5653 BstYI r'GATC_y Cuts at: 1972 2983 3350 3735 3752 5204 5290 5302 5648 Size: 1011 367 385 17 1452 86 12 346 Cuts at: 5648 1972 Size: 1977 Fragments arranged by size: 1977 1452 1011 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2322 513 Size: 552 1257 3844 Fragments arranged by size: 3844 1257 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4914 Fragments arranged by size: 4914 683 56 BtsI GCAGTG_nn' Cuts at: 2576 3169 3963 3983 2576 Size: 593 794 20 4246 Fragments arranged by size: 4246 794 593 20 ClaI AT'CG_AT Cuts at: 1554 2523 1554 Size: 969 4684 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5618 DraI TTT'AAA Cuts at: 2957 2957 Size: 5653 DraIII CAC_nnn'GTG Cuts at: 2337 2337 Size: 5653 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4660 809 Size: 507 3344 1802 Fragments arranged by size: 3344 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2349 2376 3100 Size: 54 1425 50 275 18 519 27 724 Cuts at: 3100 3989 5618 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 724 519 275 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 3100 1537 Size: 275 1288 4090 Fragments arranged by size: 4090 1288 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2090 3583 1353 Size: 737 1493 3423 Fragments arranged by size: 3423 1493 737 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4240 4614 4760 387 Size: 1064 138 2651 374 146 1280 Fragments arranged by size: 2651 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2077 3716 4313 5100 5368 1239 Size: 393 445 1639 597 787 268 1524 Fragments arranged by size: 1639 1524 787 597 445 393 268 EcoRI G'AATT_C Cuts at: 2962 3340 2962 Size: 378 5275 FspI TGC'GCA Cuts at: 4159 4159 Size: 5653 HaeII r_GCGC'y Cuts at: 1678 4800 1678 Size: 3122 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2388 2420 4294 4326 4368 Size: 32 135 32 816 32 1874 32 42 Cuts at: 4368 4400 1373 Size: 32 2626 Fragments arranged by size: 2626 1874 816 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2997 3243 669 Size: 988 281 1059 246 3079 Fragments arranged by size: 3079 1059 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5653 HpaI GTT'AAC Cuts at: 3243 3243 Size: 5653 KpnI G_GTAC'C Cuts at: 2978 2978 Size: 5653 MfeI C'AATT_G Cuts at: 3252 3252 Size: 5653 MluI A'CGCG_T Cuts at: 5529 5529 Size: 5653 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4767 4951 1305 Size: 3462 184 2007 Fragments arranged by size: 3462 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2378 10 Size: 54 1425 343 546 3285 Fragments arranged by size: 3285 1425 546 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2408 2594 2600 3611 3970 4129 518 Size: 648 1242 186 6 1011 359 159 2042 Fragments arranged by size: 2042 1242 1011 648 359 186 159 6 MspA1I CmG'CkG Cuts at: 1606 2392 3069 3746 4894 5139 1606 Size: 786 677 677 1148 245 2120 Fragments arranged by size: 2120 1148 786 677 677 245 NcoI C'CATG_G Cuts at: 513 1065 2322 513 Size: 552 1257 3844 Fragments arranged by size: 3844 1257 552 NdeI CA'TA_TG Cuts at: 387 2362 387 Size: 1975 3678 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5653 NotI GC'GGCC_GC Cuts at: 3100 3100 Size: 5653 NspI r_CATG'y Cuts at: 1132 1229 3011 4556 1132 Size: 97 1782 1545 2229 Fragments arranged by size: 2229 1782 1545 97 PasI CC'CwG_GG Cuts at: 1348 2066 1348 Size: 718 4935 PciI A'CATG_T Cuts at: 4552 4552 Size: 5653 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5653 PmeI GTTT'AAAC Cuts at: 2957 2957 Size: 5653 PmlI CAC'GTG Cuts at: 2535 2535 Size: 5653 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3722 3754 5235 5267 3722 Size: 32 1481 32 4108 Fragments arranged by size: 4108 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5653 PsiI TTA'TAA Cuts at: 3223 3223 Size: 5653 PstI C_TGCA'G Cuts at: 830 2499 3005 830 Size: 1669 506 3478 Fragments arranged by size: 3478 1669 506 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5653 SalI G'TCGA_C Cuts at: 1936 2995 1936 Size: 1059 4594 SbfI CC_TGCA'GG Cuts at: 3005 3005 Size: 5653 ScaI AGT'ACT Cuts at: 3901 5613 3901 Size: 1712 3941 SfcI C'TryA_G Cuts at: 826 1045 2495 3001 4136 4817 5008 826 Size: 219 1450 506 1135 681 191 1471 Fragments arranged by size: 1471 1450 1135 681 506 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5653 SmaI CCC'GGG Cuts at: 1872 2980 1872 Size: 1108 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3757 4658 4920 820 Size: 197 926 1814 901 262 1553 Fragments arranged by size: 1814 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3444 493 Size: 2951 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5653 SphI G_CATG'C Cuts at: 1132 3011 1132 Size: 1879 3774 SspI AAT'ATT Cuts at: 5 52 3577 5 Size: 47 3525 2081 Fragments arranged by size: 3525 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5653 StyI C'CwwG_G Cuts at: 513 1065 2191 2322 3092 513 Size: 552 1126 131 770 3074 Fragments arranged by size: 3074 1126 770 552 131 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2280 2440 3680 3697 3850 4035 4480 Size: 1255 700 160 1240 17 153 185 445 Cuts at: 4480 325 Size: 1498 Fragments arranged by size: 1498 1255 1240 700 445 185 160 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 2299 3899 Size: 274 80 33 51 1443 204 118 1600 Cuts at: 3899 5611 96 Size: 1712 138 Fragments arranged by size: 1712 1600 1443 274 204 138 118 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5613 5645 5613 Size: 32 5621 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5653 XbaI T'CTAG_A Cuts at: 2989 2989 Size: 5653 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2599 2599 Size: 5653 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5653 XmaI C'CCGG_G Cuts at: 1870 2978 1870 Size: 1108 4545 XmnI GAAnn'nnTTC Cuts at: 2079 3782 2079 Size: 1703 3950 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3461 276 Size: 53 83 186 936 1927 2468 Fragments arranged by size: 2468 1927 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI