(Linear) (Six-base) MAPSORT of: KIEE5445.seq Check: 695 from: 1 to: 948 KIEE5445.orf With 182 enzymes: SgfI * January 29, 2014 12:01 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 72 948 Size: 72 876 AflIII A'CryG_T Cuts at: 0 529 948 Size: 529 419 ApaLI G'TGCA_C Cuts at: 0 422 948 Size: 422 526 ApoI r'AATT_y Cuts at: 0 536 948 Size: 536 412 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 286 319 948 Size: 286 33 629 Fragments arranged by size: 629 286 33 BanI G'GyrC_C Cuts at: 0 721 948 Size: 721 227 BanII G_rGCy'C Cuts at: 0 325 948 Size: 325 623 BbsI GAAGACnn'nnnn_ Cuts at: 0 78 948 Size: 78 870 BbvCI CC'TCA_GC Cuts at: 0 684 948 Size: 684 264 BclI T'GATC_A Cuts at: 0 140 948 Size: 140 808 BglI GCCn_nnn'nGGC Cuts at: 0 352 948 Size: 352 596 Bme1580I G_kGCm'C Cuts at: 0 426 726 948 Size: 426 300 222 Fragments arranged by size: 426 300 222 BmrI ACTGGGnnnn_n' Cuts at: 0 48 948 Size: 48 900 Bpu10I CC'TnA_GC Cuts at: 0 684 948 Size: 684 264 BsaI GGTCTCn'nnnn_ Cuts at: 0 444 948 Size: 444 504 BsaAI yAC'GTr Cuts at: 0 530 948 Size: 530 418 BsaBI GATnn'nnATC Cuts at: 0 145 948 Size: 145 803 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 601 948 Size: 601 347 BseYI C'CCAG_C Cuts at: 0 909 948 Size: 909 39 BsiHKAI G_wGCw'C Cuts at: 0 341 426 948 Size: 341 85 522 Fragments arranged by size: 522 341 85 BsmI GAATG_Cn' Cuts at: 0 605 948 Size: 605 343 Bsp1286I G_dGCh'C Cuts at: 0 325 341 426 726 948 Size: 325 16 85 300 222 Fragments arranged by size: 325 300 222 85 16 BspHI T'CATG_A Cuts at: 0 404 578 948 Size: 404 174 370 Fragments arranged by size: 404 370 174 BsrBI CCG'CTC Cuts at: 0 466 948 Size: 466 482 BsrDI GCAATG_nn' Cuts at: 0 922 948 Size: 922 26 BsrGI T'GTAC_A Cuts at: 0 294 948 Size: 294 654 BstXI CCAn_nnnn'nTGG Cuts at: 0 594 948 Size: 594 354 BtgI C'CryG_G Cuts at: 0 317 948 Size: 317 631 BtsI GCAGTG_nn' Cuts at: 0 571 948 Size: 571 377 ClaI AT'CG_AT Cuts at: 0 518 948 Size: 518 430 DraIII CAC_nnn'GTG Cuts at: 0 332 948 Size: 332 616 EaeI y'GGCC_r Cuts at: 0 344 371 948 Size: 344 27 577 Fragments arranged by size: 577 344 27 EarI CTCTTCn'nnn_ Cuts at: 0 85 948 Size: 85 863 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 72 948 Size: 72 876 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 383 415 948 Size: 383 32 533 Fragments arranged by size: 533 383 32 MscI TGG'CCA Cuts at: 0 373 948 Size: 373 575 MslI CAynn'nnrTG Cuts at: 0 403 589 595 948 Size: 403 186 6 353 Fragments arranged by size: 403 353 186 6 MspA1I CmG'CkG Cuts at: 0 387 948 Size: 387 561 NcoI C'CATG_G Cuts at: 0 317 948 Size: 317 631 NdeI CA'TA_TG Cuts at: 0 357 948 Size: 357 591 PasI CC'CwG_GG Cuts at: 0 61 948 Size: 61 887 PmlI CAC'GTG Cuts at: 0 530 948 Size: 530 418 PstI C_TGCA'G Cuts at: 0 494 948 Size: 494 454 SfcI C'TryA_G Cuts at: 0 490 948 Size: 490 458 StyI C'CwwG_G Cuts at: 0 186 317 948 Size: 186 131 631 Fragments arranged by size: 631 186 131 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 275 435 948 Size: 275 160 513 Fragments arranged by size: 513 275 160 TatI w'GTAC_w Cuts at: 0 176 294 948 Size: 176 118 654 Fragments arranged by size: 654 176 118 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 594 948 Size: 594 354 XmnI GAAnn'nnTTC Cuts at: 0 74 948 Size: 74 874 Enzymes that do cut: AcuI AflIII ApaLI ApoI BaeI BanI BanII BbsI BbvCI BclI BglI Bme1580I BmrI Bpu10I BsaI BsaAI BsaBI BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI BsrBI BsrDI BsrGI BstXI BtgI BtsI ClaI DraIII EaeI EarI Eco57MI Hin4I MscI MslI MspA1I NcoI NdeI PasI PmlI PstI SfcI StyI TaqII TatI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaI AscI AseI AsiSI AvaI AvrII BamHI BbeI BcgI BciVI BfrBI BglII BlpI BmgBI BmtI BplI BpmI BpuEI BsaHI BsaWI BsaXI BsgI BsiEI BsiWI BsmBI BspEI BspMI BsrFI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI CspCI DraI DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XhoI XmaI ZraI