################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## Acc65I AccI AclI AlwNI Aor51HI AscI Asp718I AspI AviII AvrII BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BglI BglII BlnI BlpI Bpu1102I BpuAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspLU11I BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstXI BstZ17I CelII ClaI CpoI EagI EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I FseI FspI HaeII HindIII KasI KpnI MfeI MluI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RsrII SacI SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AatII 2287 GACGTC GACGT^C AccIII 1869 TCCGGA T^CCGGA AflII 1573 CTTAAG C^TTAAG AflIII 1241 ACACGT A^C[GA][CT]GT AgeI 2545 ACCGGT A^CCGGT AocI 943 CCTCAGG CC^T[ACGT]AGG ApaI 2155 GGGCCC GGGCC^C BfrI 1573 CTTAAG C^TTAAG BseAI 1869 TCCGGA T^CCGGA BsmI 1646 GCATTC GAATGC(1/-1) BspEI 1869 TCCGGA T^CCGGA BspHI 2291 TCATGA T^CATGA BsrBI 1797 CCGCTC CCGCTC(-3/-3) BsrFI 2545 ACCGGT [GA]^CCGG[CT] BssHII 2640 GCGCGC G^CGCGC BstEII 1449 GGTGACC G^GT[ACGT]ACC BstPI 1449 GGTGACC G^GT[ACGT]ACC Bsu36I 943 CCTCAGG CC^T[ACGT]AGG Cfr10I 2545 ACCGGT [GA]^CCGG[CT] DraI 127 TTTAAA TTT^AAA DrdI 1350 GACCCAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1179 CTCTTC CTCTTC(1/4) Eco81I 943 CCTCAGG CC^T[ACGT]AGG EcoO65I 1449 GGTGACC G^GT[ACGT]ACC HpaI 414 GTTAAC GTT^AAC Ksp632I 1179 CTCTTC CTCTTC(1/4) KspI 713 CCGCGG CCGC^GG MroI 1869 TCCGGA T^CCGGA MspA1I 713 CCGCGG C[AC]G^C[GT]G NcoI 1898 CCATGG C^CATGG NspI 860 ACATGC [GA]CATG^[CT] PflMI 2721 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 2545 ACCGGT A^CCGGT RcaI 2291 TCATGA T^CATGA SacII 713 CCGCGG CCGC^GG SmaI 2054 CCCGGG CCC^GGG Van91I 2721 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 2054 CCCGGG C^CCGGG XmaI 2054 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcyI 1474 GACGCC G[GA]^CG[CT]C 2287 GACGTC AhdI 1810 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2253 GACCAAATGTC Alw44I 172 GTGCAC G^TGCAC 2174 GTGCAC ApaLI 172 GTGCAC G^TGCAC 2174 GTGCAC AseI 34 ATTAAT AT^TAAT 2086 ATTAAT AsnI 34 ATTAAT AT^TAAT 2086 ATTAAT AspEI 1810 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2253 GACCAAATGTC AvaI 2054 CCCGGG C^[CT]CG[GA]G 2354 CCCGAG AvaII 635 GGACC G^G[AT]CC 2252 GGACC BamHI 1894 GGATCC G^GATCC 2730 GGATCC BclI 1419 TGATCA T^GATCA 1778 TGATCA BpmI 1391 CTCCAG CTGGAG(16/14) 2410 CTCCAG BsaHI 1474 GACGCC G[GA]^CG[CT]C 2287 GACGTC BsaI 2633 GAGACC GGTCTC(1/5) 2486 GGTCTC BsaWI 1869 TCCGGA [AT]^CCGG[AT] 2545 ACCGGT BsoBI 2054 CCCGGG C^[CT]CG[GA]G 2354 CCCGAG Eam1105I 1810 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2253 GACCAAATGTC EcoT14I 760 CCTTGG C^C[AT][AT]GG 1898 CCATGG FbaI 1419 TGATCA T^GATCA 1778 TGATCA HgaI 1474 GACGC GACGC(5/10) 448 GCGTC Hin1I 1474 GACGCC G[GA]^CG[CT]C 2287 GACGTC HincII 414 GTTAAC GT[CT]^[GA]AC 2226 GTTGAC HindII 414 GTTAAC GT[CT]^[GA]AC 2226 GTTGAC PshBI 34 ATTAAT AT^TAAT 2086 ATTAAT SfaNI 1596 GATGC GCATC(5/9) 95 GCATC SmlI 1265 CTCAAG C^T[CT][GA]AG 1573 CTTAAG SspI 203 AATATT AAT^ATT 1016 AATATT StyI 760 CCTTGG C^C[AT][AT]GG 1898 CCATGG XcmI 99 CCACTTTTTAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2600 CCATTTACCCACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 93 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI Asp700I AspHI BalI BanII BbvI BcnI BfaI BmyI BsaAI BsaBI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScaI ScrFI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI