(Circular) (Six-base) MAPSORT of: pFN21AE5814.seq Check: 6566 from: 1 to: 7444 pFN21AE5814 7444 bp With 182 enzymes: SgfI * December 8, 2014 13:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3646 3656 3646 Size: 10 7434 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4296 5254 278 Size: 53 83 186 936 2760 958 2468 Fragments arranged by size: 2760 2468 958 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4787 1937 Size: 2850 4594 Acc65I G'GTAC_C Cuts at: 4765 4765 Size: 7444 AclI AA'CG_TT Cuts at: 1563 5571 5944 1563 Size: 4008 373 3063 Fragments arranged by size: 4008 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2414 3306 3846 5507 6891 7159 1632 Size: 782 892 540 1661 1384 268 1917 Fragments arranged by size: 1917 1661 1384 892 782 540 268 AflII C'TTAA_G Cuts at: 820 1017 3578 820 Size: 197 2561 4686 Fragments arranged by size: 4686 2561 197 AflIII A'CryG_T Cuts at: 1203 3246 6343 7320 1203 Size: 2043 3097 977 1327 Fragments arranged by size: 3097 2043 1327 977 AgeI A'CCGG_T Cuts at: 4550 6275 4550 Size: 1725 5719 AhdI GACnn_n'nnGTC Cuts at: 3820 4263 6173 3820 Size: 443 1910 5091 Fragments arranged by size: 5091 1910 443 AleI CACnn'nnGTG Cuts at: 1166 2190 4530 1166 Size: 1024 2340 4080 Fragments arranged by size: 4080 2340 1024 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2635 4194 1184 Size: 1451 1559 4434 Fragments arranged by size: 4434 1559 1451 AlwNI CAG_nnn'CTG Cuts at: 5187 6759 5187 Size: 1572 5872 ApaI G_GGCC'C Cuts at: 4164 4164 Size: 7444 ApaLI G'TGCA_C Cuts at: 2177 4179 5501 6657 2177 Size: 2002 1322 1156 2964 Fragments arranged by size: 2964 2002 1322 1156 ApoI r'AATT_y Cuts at: 1493 2784 3563 4012 4116 4472 4753 4979 5131 Size: 1291 779 449 104 356 281 226 152 Cuts at: 5131 7240 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1291 779 449 356 281 226 152 104 AseI AT'TA_AT Cuts at: 160 1032 2040 4092 5998 160 Size: 872 1008 2052 1906 1606 Fragments arranged by size: 2052 1906 1606 1008 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7444 AvaI C'yCGr_G Cuts at: 1870 1943 4059 4359 4769 1870 Size: 73 2116 300 410 4545 Fragments arranged by size: 4545 2116 410 300 73 BamHI G'GATC_C Cuts at: 3899 4735 4774 5141 3899 Size: 836 39 367 6202 Fragments arranged by size: 6202 836 367 39 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4765 6220 618 Size: 325 217 627 2978 1455 1842 Fragments arranged by size: 2978 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2990 3260 3470 4164 4515 4763 1391 Size: 1599 270 210 694 351 248 4072 Fragments arranged by size: 4072 1599 694 351 270 248 210 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6473 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5635 5669 1903 Size: 34 3698 34 3678 Fragments arranged by size: 3698 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5336 6546 5336 Size: 1210 6234 BclI T'GATC_A Cuts at: 1548 3424 3783 1548 Size: 1876 359 5209 Fragments arranged by size: 5209 1876 359 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6055 136 Size: 107 122 71 631 605 4383 1525 Fragments arranged by size: 4383 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7439 7439 Size: 7444 BlpI GC'TnA_GC Cuts at: 4861 4861 Size: 7444 Bme1580I G_kGCm'C Cuts at: 1790 2181 4164 4183 4449 5505 6661 1790 Size: 391 1983 19 266 1056 1156 2573 Fragments arranged by size: 2573 1983 1156 1056 391 266 19 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7444 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5175 6133 442 Size: 416 53 83 399 24 3758 958 1753 Fragments arranged by size: 3758 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7444 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3778 3778 Size: 7444 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3381 4400 6104 1239 Size: 2142 1019 1704 2579 Fragments arranged by size: 2579 2142 1704 1019 Bpu10I CC'TnA_GC Cuts at: 3657 3657 Size: 7444 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3255 5569 6434 6732 3255 Size: 2314 865 298 3967 Fragments arranged by size: 3967 2314 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4497 4632 6107 882 Size: 617 2998 135 1475 2219 Fragments arranged by size: 2998 2219 1475 617 135 BsaAI yAC'GTr Cuts at: 493 1204 2021 3249 4105 5235 493 Size: 711 817 1228 856 1130 2702 Fragments arranged by size: 2702 1228 1130 856 817 711 BsaBI GATnn'nnATC Cuts at: 3076 3539 3737 7438 3076 Size: 463 198 3701 3082 Fragments arranged by size: 3701 3082 463 198 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3480 4293 5251 5633 Size: 53 83 186 936 1947 813 958 382 Cuts at: 5633 6268 275 Size: 635 1451 Fragments arranged by size: 1947 1451 958 936 813 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3874 4550 5877 6275 6549 6696 7107 3874 Size: 676 1327 398 274 147 411 4211 Fragments arranged by size: 4211 1327 676 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7219 Fragments arranged by size: 7219 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3600 3805 4447 1186 Size: 2414 205 642 4183 Fragments arranged by size: 4183 2414 642 205 BseYI C'CCAG_C Cuts at: 3058 6647 7143 3058 Size: 3589 496 3359 Fragments arranged by size: 3589 3359 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2651 2669 4324 4679 1163 Size: 573 915 18 1655 355 3928 Fragments arranged by size: 3928 1655 915 573 355 18 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4894 5655 6683 1540 Size: 275 186 2893 761 1028 2301 Fragments arranged by size: 2893 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2181 2916 3090 4183 5505 5590 6661 2181 Size: 735 174 1093 1322 85 1071 2964 Fragments arranged by size: 2964 1322 1093 1071 735 174 85 BsmI GAATG_Cn' Cuts at: 3651 4955 5048 3651 Size: 1304 93 6047 Fragments arranged by size: 6047 1304 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2181 2916 2990 3090 3260 3470 4164 Size: 399 391 735 74 100 170 210 694 Cuts at: 4164 4183 4449 4515 4763 5505 5590 6661 1391 Size: 19 266 66 248 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 735 694 399 391 266 248 210 170 100 85 74 66 19 BspEI T'CCGG_A Cuts at: 3874 7107 3874 Size: 3233 4211 BspHI T'CATG_A Cuts at: 4296 5333 7063 4296 Size: 1037 1730 4677 Fragments arranged by size: 4677 1730 1037 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2432 3646 3656 4183 4608 4799 844 Size: 1588 1214 10 527 425 191 3489 Fragments arranged by size: 3489 1588 1214 527 425 191 10 BsrBI CCG'CTC Cuts at: 1394 1823 3804 5331 1394 Size: 429 1981 1527 3507 Fragments arranged by size: 3507 1981 1527 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5939 6113 65 Size: 1183 4691 174 1396 Fragments arranged by size: 4691 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4550 6088 6275 1700 Size: 110 2740 1538 187 2869 Fragments arranged by size: 2869 2740 1538 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7444 BssHII G'CGCG_C Cuts at: 1925 4645 1925 Size: 2720 4724 BssSI C'ACGA_G Cuts at: 5504 6516 5504 Size: 1012 6432 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7444 BstBI TT'CG_AA Cuts at: 6248 6248 Size: 7444 BstEII G'GTnAC_C Cuts at: 1184 3454 1184 Size: 2270 5174 BstYI r'GATC_y Cuts at: 1972 2680 3071 3899 4282 4735 4774 5141 5526 Size: 708 391 828 383 453 39 367 385 Cuts at: 5526 5543 6995 7081 7093 7439 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 828 708 453 391 385 383 367 346 86 39 17 12 Bsu36I CC'TnA_GG Cuts at: 2949 2949 Size: 7444 BtgI C'CryG_G Cuts at: 513 1065 2238 2718 3903 4303 4438 513 Size: 552 1173 480 1185 400 135 3519 Fragments arranged by size: 3519 1185 1173 552 480 400 135 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4132 4330 487 Size: 683 56 2906 198 3601 Fragments arranged by size: 3601 2906 683 198 56 BtsI GCAGTG_nn' Cuts at: 2351 2448 4525 4537 4960 5754 5774 2351 Size: 97 2077 12 423 794 20 4021 Fragments arranged by size: 4021 2077 794 423 97 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7444 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7409 DraI TTT'AAA Cuts at: 2134 4748 2134 Size: 2614 4830 DraIII CAC_nnn'GTG Cuts at: 3454 4405 4444 3454 Size: 951 39 6454 Fragments arranged by size: 6454 951 39 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3361 6451 809 Size: 507 2045 3090 1802 Fragments arranged by size: 3090 2045 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2155 3694 3984 Size: 54 1425 50 275 18 325 1539 290 Cuts at: 3984 4277 4891 5780 7409 8 Size: 293 614 889 1629 43 Fragments arranged by size: 1629 1539 1425 889 614 325 293 290 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4891 1537 Size: 275 3079 4090 Fragments arranged by size: 4090 3079 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3190 5374 1353 Size: 1837 2184 3423 Fragments arranged by size: 3423 2184 1837 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4270 6031 6405 6551 387 Size: 1064 138 2681 1761 374 146 1280 Fragments arranged by size: 2681 1761 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2414 3306 3381 3846 4400 5507 6104 Size: 393 782 892 75 465 554 1107 597 Cuts at: 6104 6891 7159 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1107 892 787 782 597 554 465 393 268 75 EcoO109I rG'GnC_Cy Cuts at: 3922 4160 4161 3922 Size: 238 1 7205 Fragments arranged by size: 7205 238 1 EcoRI G'AATT_C Cuts at: 4753 5131 4753 Size: 378 7066 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4715 4715 Size: 7444 FspI TGC'GCA Cuts at: 5950 5950 Size: 7444 HaeII r_GCGC'y Cuts at: 1678 6591 1678 Size: 4913 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2073 2105 2219 2251 3308 Size: 32 135 32 501 32 114 32 1057 Cuts at: 3308 3340 3806 3838 4249 4281 4442 4474 6085 Size: 32 466 32 411 32 161 32 1611 Cuts at: 6085 6117 6159 6191 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1611 1057 501 466 411 161 135 114 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2421 4233 4788 5034 669 Size: 988 281 483 1812 555 246 3079 Fragments arranged by size: 3079 1812 988 555 483 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7444 HpaI GTT'AAC Cuts at: 2421 5034 2421 Size: 2613 4831 KpnI G_GTAC'C Cuts at: 4769 4769 Size: 7444 MfeI C'AATT_G Cuts at: 5043 5043 Size: 7444 MluI A'CGCG_T Cuts at: 7320 7320 Size: 7444 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4485 6558 6742 1305 Size: 3180 2073 184 2007 Fragments arranged by size: 3180 2073 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2157 3986 4279 10 Size: 54 1425 343 325 1829 293 3175 Fragments arranged by size: 3175 1829 1425 343 325 293 54 MslI CAynn'nnrTG Cuts at: 518 1166 2190 3717 4176 4226 4530 4655 5402 Size: 648 1024 1527 459 50 304 125 747 Cuts at: 5402 5761 5920 518 Size: 359 159 2042 Fragments arranged by size: 2042 1527 1024 747 648 459 359 304 159 125 50 MspA1I CmG'CkG Cuts at: 1606 2720 4860 5537 6685 6930 1606 Size: 1114 2140 677 1148 245 2120 Fragments arranged by size: 2140 2120 1148 1114 677 245 NcoI C'CATG_G Cuts at: 513 1065 3903 513 Size: 552 2838 4054 Fragments arranged by size: 4054 2838 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7444 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7444 NotI GC'GGCC_GC Cuts at: 4891 4891 Size: 7444 NspI r_CATG'y Cuts at: 1132 1229 2869 4802 6347 1132 Size: 97 1640 1933 1545 2229 Fragments arranged by size: 2229 1933 1640 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7444 PciI A'CATG_T Cuts at: 6343 6343 Size: 7444 PflMI CCAn_nnn'nTGG Cuts at: 1108 4732 1108 Size: 3624 3820 PmeI GTTT'AAAC Cuts at: 4748 4748 Size: 7444 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5513 5545 7026 7058 5513 Size: 32 1481 32 5899 Fragments arranged by size: 5899 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7444 PsiI TTA'TAA Cuts at: 5014 5014 Size: 7444 PspOMI G'GGCC_C Cuts at: 4160 4160 Size: 7444 PstI C_TGCA'G Cuts at: 830 2358 3193 4532 4796 830 Size: 1528 835 1339 264 3478 Fragments arranged by size: 3478 1528 1339 835 264 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7444 SacII CC_GC'GG Cuts at: 2721 2721 Size: 7444 SalI G'TCGA_C Cuts at: 1936 4786 1936 Size: 2850 4594 SbfI CC_TGCA'GG Cuts at: 4796 4796 Size: 7444 ScaI AGT'ACT Cuts at: 2975 3491 4274 5692 7404 2975 Size: 516 783 1418 1712 3015 Fragments arranged by size: 3015 1712 1418 783 516 SfcI C'TryA_G Cuts at: 826 1045 2354 3189 3964 4528 4792 5927 6608 Size: 219 1309 835 775 564 264 1135 681 Cuts at: 6608 6799 826 Size: 191 1471 Fragments arranged by size: 1471 1309 1135 835 775 681 564 264 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7444 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7444 SmaI CCC'GGG Cuts at: 1872 4061 4771 1872 Size: 2189 710 4545 Fragments arranged by size: 4545 2189 710 SmlI C'TyrA_G Cuts at: 820 1017 1943 3270 3578 5548 6449 6711 820 Size: 197 926 1327 308 1970 901 262 1553 Fragments arranged by size: 1970 1553 1327 926 901 308 262 197 SnaBI TAC'GTA Cuts at: 493 5235 493 Size: 4742 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7444 SphI G_CATG'C Cuts at: 1132 4802 1132 Size: 3670 3774 SspI AAT'ATT Cuts at: 5 52 2210 3023 5368 5 Size: 47 2158 813 2345 2081 Fragments arranged by size: 2345 2158 2081 813 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7444 StyI C'CwwG_G Cuts at: 513 1065 2765 3903 4883 513 Size: 552 1700 1138 980 3074 Fragments arranged by size: 3074 1700 1138 980 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2018 2180 2758 4397 5471 5488 5641 Size: 1255 438 162 578 1639 1074 17 153 Cuts at: 5641 5826 6271 325 Size: 185 445 1498 Fragments arranged by size: 1639 1498 1255 1074 578 445 438 185 162 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2973 3489 4143 Size: 274 80 33 51 1443 996 516 654 Cuts at: 4143 4272 4674 5690 7402 96 Size: 129 402 1016 1712 138 Fragments arranged by size: 1712 1443 1016 996 654 516 402 274 138 129 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2890 2922 3637 3669 7404 7436 2890 Size: 32 715 32 3735 32 2898 Fragments arranged by size: 3735 2898 715 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7444 XbaI T'CTAG_A Cuts at: 4780 4780 Size: 7444 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2111 4612 2111 Size: 2501 4943 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7444 XmaI C'CCGG_G Cuts at: 1870 4059 4769 1870 Size: 2189 710 4545 Fragments arranged by size: 4545 2189 710 XmnI GAAnn'nnTTC Cuts at: 2713 2804 4695 5573 2713 Size: 91 1891 878 4584 Fragments arranged by size: 4584 1891 878 91 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4294 5252 276 Size: 53 83 186 936 2760 958 2468 Fragments arranged by size: 2760 2468 958 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI